Chloroplast simple sequence repeat (cpSSR) markers in Citrus were developed and successfully used to analyze chloroplast genome inheritance of Citrus somatic hybrids. Twenty-two previously reported cpSSR primer pairs ...Chloroplast simple sequence repeat (cpSSR) markers in Citrus were developed and successfully used to analyze chloroplast genome inheritance of Citrus somatic hybrids. Twenty-two previously reported cpSSR primer pairs from pine (Pinus thunbergii Parl.), rice (Otyza sativa L.) and tobacco (Nicotiana tabacum L.) were tested in Citrus, nine of which could amplify intensive PCR products by agarose gel electrophoresis. Chloroplast genome inheritance of Citrus somatic hybrids from nine fusions was then analyzed, and five of the nine pre-screened primer pairs showed polymorphisms by polyacrylamide gel electrophoresis. The results revealed the random inheritance nature of chloroplast genome in all analyzed Citrus somatic hybrids, which was in agreement with previous reports based on RFLP or CAPS analyses. It was also shown that cpSSR is a more efficient tool in chloroplast genome analyses of somatic hybrids in higher plants, compared with the conventional RFLP or CAPS analyses.展开更多
Oryza sativa and Oryza latifolia belong to the AA and CCDD genomes of Oryza, respectively. In this study, amphiploids were obtained from the tube seedlings of O. sativa × O. latifolia F1 hybrids by treatment with...Oryza sativa and Oryza latifolia belong to the AA and CCDD genomes of Oryza, respectively. In this study, amphiploids were obtained from the tube seedlings of O. sativa × O. latifolia F1 hybrids by treatment with colchicine, an agent for chromosome doubling. Subse- quently, amphiploids were investigated using the methods of morphology, genomic in situ hybridization, and molec- ular markers. Amphiploids were characterized by a shorter plant height, larger diameter of stem, longer and wider leaves, darker leaf color, decreased spikelets per panicle and panicle length, and larger spikelets and anthers than the original F1 hybrid. Based on the mitotic metaphase chro- mosome number of the investigated root tips, the somatic chromosome number of the amphiploid is 2n = 72. Additionally, the amphiploid is an allohexaploid, and its genomic constitution is AACCDD by genomic in situ hybridization analysis. Finally, the amphiploids were identified to be true using 37 polymorphic markers at the DNA level.展开更多
文摘Chloroplast simple sequence repeat (cpSSR) markers in Citrus were developed and successfully used to analyze chloroplast genome inheritance of Citrus somatic hybrids. Twenty-two previously reported cpSSR primer pairs from pine (Pinus thunbergii Parl.), rice (Otyza sativa L.) and tobacco (Nicotiana tabacum L.) were tested in Citrus, nine of which could amplify intensive PCR products by agarose gel electrophoresis. Chloroplast genome inheritance of Citrus somatic hybrids from nine fusions was then analyzed, and five of the nine pre-screened primer pairs showed polymorphisms by polyacrylamide gel electrophoresis. The results revealed the random inheritance nature of chloroplast genome in all analyzed Citrus somatic hybrids, which was in agreement with previous reports based on RFLP or CAPS analyses. It was also shown that cpSSR is a more efficient tool in chloroplast genome analyses of somatic hybrids in higher plants, compared with the conventional RFLP or CAPS analyses.
基金supported by the National Natural Science Foundation of China (31571624, 31071382)the National Basic Research Program of China (2010CB125904, 2013CBA01405)+1 种基金the key Natural Science Project in University of Jiangsu Province (15KJA210004)the Priority Academic Program Development of Jiangsu Higher Education Institutions
文摘Oryza sativa and Oryza latifolia belong to the AA and CCDD genomes of Oryza, respectively. In this study, amphiploids were obtained from the tube seedlings of O. sativa × O. latifolia F1 hybrids by treatment with colchicine, an agent for chromosome doubling. Subse- quently, amphiploids were investigated using the methods of morphology, genomic in situ hybridization, and molec- ular markers. Amphiploids were characterized by a shorter plant height, larger diameter of stem, longer and wider leaves, darker leaf color, decreased spikelets per panicle and panicle length, and larger spikelets and anthers than the original F1 hybrid. Based on the mitotic metaphase chro- mosome number of the investigated root tips, the somatic chromosome number of the amphiploid is 2n = 72. Additionally, the amphiploid is an allohexaploid, and its genomic constitution is AACCDD by genomic in situ hybridization analysis. Finally, the amphiploids were identified to be true using 37 polymorphic markers at the DNA level.
