量子高科“水源基因” 发现肌肤不老“水”能量

肌肤如何才能逃离时间的魔掌？让细纹、松弛的肌肤恢复如婴儿般水润？量子高科“水源基因”生物因子系列美容品，“让专业美容变得更快速、更方便、更简单”，10秒钟即可奉献最科学、专业的美容调养，其中，应用Biotablet^TM技术的生物活性冻千闪释片更成为爱美女性的“便携式美容院”。

Transfer of Lysine-rich Protein Gene into Rice and Production of Fertile Transgenic Plants

Lysine-rich protein gene (lys) was cloned from Psophocarpus tetragonolobus (L.) DC. A plant expression plasmid was constructed and lys gene was under the control of maize ubiquitin promoter which is the highest efficient monocotyledon promoter. The plasmid was introduced into rice embryogenic calli by microprojectile bombardment. The regenerated fertile plants were obtained by effective selection for hygromycin B resistance. Genomic PCR and Southern blotting analyses showed that the lys gene has been integrated into rice genome. Simultaneously, the results of GUS histochemical assay demonstrated that gus report gene is also expressed in leaves, stems and roots of the transgenic rice plants. Data analysis showed that lysine content in most of the 11 transgenic plants is differently improved, and in one of them increased by 16.04%.

Effects of Exogenous Abscisic Acid and Water Stress on the Growth Response of Subterranean Clover of Different Genotypes

The response of subterranean clover ( Trifolium subterraneum L.) to different abscisic acid (ABA) concentrations (10 -6 , 10 -5 , 10 -4 mol/L) in nutrient solution and to water stress were studied in growth room. Six cultivars of contrasting yield capacity were compared. Plants remained growing in Hoagland solution or pots until at least four full_developed leaves appeared. The ABA was then applied and the fresh weight, leaf number and length of the largest root were measured at 1, 4, 7 and 11 d after ABA treatment. The above parameters were also measured at 15 d under water stress. In all the tested genotypes ABA caused similar reduction in these growth parameters, as well as a significant decrease of leaf water potential which was dependent on ABA concentration. The average growth reduction after 11 d under 10 -4 mol/L ABA coincided with the range of these crops under water stress in pot experiments. On average of the different genotypes, leaf number, area of full_developed leaf and the dry weight per plant decreased by about 50% whereas the root/shoot ratio increased by 80%.The genotype variation and ranking for this treatment were rather similar to the same genotypes in pot experiments. The genotypes, Clare, Nuba and Seaton Park, showed the best results under both control and ABA treated conditions and water stress conditions. The similarity between the response to ABA in nutrient solution and to water stress opens the possibility to use this approach as a way to quantify the drought resistance of subterranean clover genotypes.

Cloning and Expression Analysis of a Human Putative Tumor Suppressor Gene Homologue from Rice

A gene homologous to the human Putative tumor suppressor gone QM, designated OSQM1, was isolated from rice (Oryza sativa L.) genomic DNA library using through homology screening. It contained 4 exons and 3 introns, encoding a protein of 219 amino acids with 46 basic amino acids, leading to a high isoelectric point of 11.02. Homology search showed that this gene existed in eukaryotes and highly conserved throughout eukaryotes, suggesting an essential role of this gene. Northern Not analysis showed that it was expressed in various rice organs, but at lower level in developing flower and callus tissue than in other vegetative organs. Its expression levels in roots and leaves were influenced by different environmental factors.

Perspectives:Gene expression in fisheries management

Functional genes and gene expression have been connected to physiological traits linked to effective production and broodstock selection in aquaculture, selective implications of commercial fish harvest, and adaptive changes reflected in non-commercial fish populations subject to human disturbance and climate change. Gene mapping using single nucleotide polymorphisms （SNPs） to identify functional genes, gene expression （analogue microarrays and real-time PCR）, and digital sequencing technologies looking at RNA transcripts present new concepts and opportunities in support of effective and sustainable fisheries. Genomic tools have been rapidly growing in aquaculture research addressing aspects of fish health, toxicology, and early development. Genomic technologies linking effects in functional genes involved in growth, maturation and life history development have been tied to selection resulting from harvest practices. Incorporating new and ever-increasing knowledge of fish gehomes is opening a different perspective on local adaptation that will prove invaluable in wild fish conservation and management. Conservation of fish stocks is rapidly incorporating research on critical adaptive responses directed at the effects of human dis- turbance and climate change through gene expression studies. Genomic studies of fish populations can be generally grouped into three broad categories： l） evolutionary genomics and biodiversity; 2） adaptive physiological responses to a changing environment; and 3） adaptive behavioral genomics and life history diversity. We review current genomic research in fisheries focusing on those that use microarrays to explore differences in gene expression among phenotypes and within or across populations, information that is critically important to the conservation of fish and their relationship to humans [Current Zoology 56 （1）： 157-174, 2010].

Molecular breeding of hybrid rice

This paper describes in a general way the objectives, contents and methods of molecular breeding, and the application of molecular breeding in hybrid rice.

Knockdown of OsHox33, a member of the class III homeodomain-leucine zipper gene family, accelerates leaf senescence in rice

The class III homeodomain-leucine zipper(HD-Zip III)gene family plays important roles in plant growth and development,including regulation of apical embryo patterning,embryonic shoot meristem formation,leaf polarity,vascular development,and meristem function,with a particularly crucial function in leaf development.Although HD-Zip III members are highly conserved in land plants,previous studies,such as genetic analyses based on multiple mutants in Arabidopsis and other plants,suggest that various HD-Zip III family genes have evolved with distinct functions and pleiotropic effects on plant growth and development.In this study,we analyzed a HD-Zip III member,OsHox33,and demonstrated that it plays an important role in age-dependent leaf senescence in rice.We constructed two specific RNAi vectors derived from the 5′-end region and 3′-UTR of OsHox33 to knockdown its expression.Transgenic plants harboring either RNAi construct displayed similar phenotypes of precocious leaf senescence symptoms,suggesting that knockdown of OsHox33 accelerates leaf senescence in rice.pOsHox33::GUS fusion expression and RT-PCR revealed that OsHox33 is highly expressed in young organs,especially in young meristems such as shoot apical meristems,intercalary meristems,and young callus.In addition,real-time PCR indicated that OsHox33 was more highly expressed in young leaves than in old leaves.To further investigate OsHox33 function,we analyzed chloroplast ultrastructure in different-aged leaves of RNAi plants,and found that OsHox33 knockdown accelerated chloroplast degradation,which is consistent with RNAi phenotypes.Finally,real-time PCR studies showed that OsHox33 can regulate the expression of GS1 and GS2,two senescence-associated genes.Taken together,the work presented here provides new insights into the function of HD-Zip III members in plants.