AIM: To investigate the effect of Bu-Zhong-Yi-Qi-Tang (Decoction for Reinforcing Middle Jiao and Replenishing Qi) on deficiency of N-glycan/nitric oxide (NO) and islet damage induced by injecting two medium doses of s...AIM: To investigate the effect of Bu-Zhong-Yi-Qi-Tang (Decoction for Reinforcing Middle Jiao and Replenishing Qi) on deficiency of N-glycan/nitric oxide (NO) and islet damage induced by injecting two medium doses of streptozotocin (STZ). METHODS: Diabetes was induced by intraperitoneal injection of STZ at 55 mg/kg on day 1 and day 8. Islet damage was evaluated using a scoring system. Nitrite, nitrate, α-mannosidase and amylase activities were measured by colorimetry. N-glycan patterns of amylase were determined with lectin [ConA, pisum sativum agglutinin (PSA), peanut agglutinin (PNA), and lens culinaris agglutinin (LCA)] affinity precipitation method. RESULTS: Severe islet necrosis and mild islet atrophy were observed in diabetic rats. The number and size ofislets, the activities of α-mannosidase, amylase and nitrite were decreased, while the binding of PNA and LCA to amylase was increased. All of which were improved after treatment with Bu-Zhong-Yi-Qi-Tang. Islet damage was significantly correlated with nitrite, nitrate, α-mannosidase, amylase and the binding of LCA, PNA, and PSA to amylase.CONCLUSION: STZ-induced islet damage is related to N-glycan deficiency in proteins by blocking α-mannosidase activity and no deficiency, accumulation of unfolded proteins, and endoplasmic reticulum stress and activation of cellular signals, all of which are improved after treatment with Bu-Zhong-YiQi-Tang.展开更多
In this paper, the structural and single-particle motive properties of a two-dimensional dusty plasmas are investigated numerically by molecular dynamics simulation within the framework of a dissipative Yukawa model. ...In this paper, the structural and single-particle motive properties of a two-dimensional dusty plasmas are investigated numerically by molecular dynamics simulation within the framework of a dissipative Yukawa model. The pair correlation function, the mean square displacement, the static structure factor, and the bond angle correlation function characterizing the structural properties, and the velocity autocorrelation function with Fourier spectrum function characterizing the single-particle motion have been calculated for different values of coupling constant r and viscous damping constant vf. The results show that the system will coagulate quickly with increasing viscous damping constant and coupling constant, and the critical value of friction parameter decreases with increasing the coupling constant in the system.展开更多
基金Supported by The Project of Guangdong Science and Technology
文摘AIM: To investigate the effect of Bu-Zhong-Yi-Qi-Tang (Decoction for Reinforcing Middle Jiao and Replenishing Qi) on deficiency of N-glycan/nitric oxide (NO) and islet damage induced by injecting two medium doses of streptozotocin (STZ). METHODS: Diabetes was induced by intraperitoneal injection of STZ at 55 mg/kg on day 1 and day 8. Islet damage was evaluated using a scoring system. Nitrite, nitrate, α-mannosidase and amylase activities were measured by colorimetry. N-glycan patterns of amylase were determined with lectin [ConA, pisum sativum agglutinin (PSA), peanut agglutinin (PNA), and lens culinaris agglutinin (LCA)] affinity precipitation method. RESULTS: Severe islet necrosis and mild islet atrophy were observed in diabetic rats. The number and size ofislets, the activities of α-mannosidase, amylase and nitrite were decreased, while the binding of PNA and LCA to amylase was increased. All of which were improved after treatment with Bu-Zhong-Yi-Qi-Tang. Islet damage was significantly correlated with nitrite, nitrate, α-mannosidase, amylase and the binding of LCA, PNA, and PSA to amylase.CONCLUSION: STZ-induced islet damage is related to N-glycan deficiency in proteins by blocking α-mannosidase activity and no deficiency, accumulation of unfolded proteins, and endoplasmic reticulum stress and activation of cellular signals, all of which are improved after treatment with Bu-Zhong-YiQi-Tang.
基金The project supported by National Natural Science Foundation of China under Grant Nos. 10547121 and 10175036
文摘In this paper, the structural and single-particle motive properties of a two-dimensional dusty plasmas are investigated numerically by molecular dynamics simulation within the framework of a dissipative Yukawa model. The pair correlation function, the mean square displacement, the static structure factor, and the bond angle correlation function characterizing the structural properties, and the velocity autocorrelation function with Fourier spectrum function characterizing the single-particle motion have been calculated for different values of coupling constant r and viscous damping constant vf. The results show that the system will coagulate quickly with increasing viscous damping constant and coupling constant, and the critical value of friction parameter decreases with increasing the coupling constant in the system.
