AIM:Acute hepatitis may seldom have a fulminant course. In the treatment of this medical emergency,potential liver support measure must provide immediate and sufficient assistance to the hepatic function.The goal of o...AIM:Acute hepatitis may seldom have a fulminant course. In the treatment of this medical emergency,potential liver support measure must provide immediate and sufficient assistance to the hepatic function.The goal of our study was to study the adequacy of hepatocyte transplantation (HCTx)in two different anatomical sites,splenic parenchyma and peritoneal cavity,in a rat model of reversible acute hepatitis induced by carbon tetrachloride(CCl_4). METHODS:After CCl_4 intoxication,84 male Wistar rats used as recipients were divided in to four experimental groups accordingly to their treatment:Group A(n=-24):intrasplenic transplantation of 10×10~6 isolated hepatocytes,Group B(n=24): intraperitoneal transplantation of 20×10~6 isolated hepatocytes attached on plastic microcarriers,Group C(n=-18):intrasplenic injection of 1 mL normal saline(sham-operated controls), Group D(n=-18):intraperitoneal injection of 2.5 mL normal saline(sham-operated controls).Survival,liver function tests (LFT)and histology were studied in all four groups,on d 2, 5 and 10 post-HCTx. RESULTS:The ten-day survival(and mean survival)in the 4 groups was 72.2%(8.1±3.1),33.3%(5.4±3.4),0% (3.1±1.3)and 33.3%(5.4±3.6)in groups A,B,C,D, respectively(P_(AB0<0.05,P_(AC)<0.05,P_(BD)=NS).In the final survivors,LFT(except alkaline phosphatase)and hepatic histology returned to normal,independently of their previous therapy.Viable hepatocytes were identified within splenic parenchyma(in group A on d 2)and both in the native liver and the fatty tissue of abdominal wall(in group B on d 5). CONCLUSION:A significantly better survival of the intrasplenically transplanted animals has been demonstrated. Intraperitoneal hepatocytes failed to promptly engraft.A different timing between liver injury and intraperitoneal HCTx may give better results and merits further investigation.展开更多
To evaluate isotopic tracers at natural abundances by providing basic isotope data of the hydrological investigations and assessing the impacts of different factors on the water cycle, a total of 197 water samples wer...To evaluate isotopic tracers at natural abundances by providing basic isotope data of the hydrological investigations and assessing the impacts of different factors on the water cycle, a total of 197 water samples were collected from the Laohugou Glacial catchment in the Shule River basin northwestern China during the 2013 ablation seasons and analyzed their H- and O-isotope composition. The results showed that the isotopic composition of precipitation in the Qilianshan Station in the Laohugou Glacial catchment was remarkable variability. Correspondingly, a higher slope of δ180-δD diagram, with an average of 8.74, is obtained based on the precipitation samples collected on the Glacier No.la, mainly attributed to the lower temperature on the glacier surface. Because of percolation and elution, the bottom of the firn the isotopic composition at is nearly steady. The 6180 /altitude gradients for precipitation and melt water were -o.37%o/100 m and -o.34%o/100 m, respectively Exposed to the air and influenced by strong ablation and evaporation, the isotopic values and the 6180 vs 6D diagram of the glacial surface ice show no altitudinal effect, indicating that glacier ice has the similar origins with the firn. The variation of isotopic composition in the melt water, varying from -l0.7‰ to -16.9‰ （8180） and from -61.1%o to -122.1%o （6D） indicates the recharging of snowmelt and glacial ice melt water produced at different altitudes. With a mean value of -13.3‰ for 8180 and -89.7‰ for 8D, the isotopic composition of the stream water is much closer to the melt water, indicating that stream water is mainly recharged by the ablation water. Our results of the stable isotopic compositions in natural water in the Laohugou Glacial catchment indicate the fractionations and the smoothing fluctuations of the stable isotopes during evaporation, infiltration and mixture.展开更多
文摘AIM:Acute hepatitis may seldom have a fulminant course. In the treatment of this medical emergency,potential liver support measure must provide immediate and sufficient assistance to the hepatic function.The goal of our study was to study the adequacy of hepatocyte transplantation (HCTx)in two different anatomical sites,splenic parenchyma and peritoneal cavity,in a rat model of reversible acute hepatitis induced by carbon tetrachloride(CCl_4). METHODS:After CCl_4 intoxication,84 male Wistar rats used as recipients were divided in to four experimental groups accordingly to their treatment:Group A(n=-24):intrasplenic transplantation of 10×10~6 isolated hepatocytes,Group B(n=24): intraperitoneal transplantation of 20×10~6 isolated hepatocytes attached on plastic microcarriers,Group C(n=-18):intrasplenic injection of 1 mL normal saline(sham-operated controls), Group D(n=-18):intraperitoneal injection of 2.5 mL normal saline(sham-operated controls).Survival,liver function tests (LFT)and histology were studied in all four groups,on d 2, 5 and 10 post-HCTx. RESULTS:The ten-day survival(and mean survival)in the 4 groups was 72.2%(8.1±3.1),33.3%(5.4±3.4),0% (3.1±1.3)and 33.3%(5.4±3.6)in groups A,B,C,D, respectively(P_(AB0<0.05,P_(AC)<0.05,P_(BD)=NS).In the final survivors,LFT(except alkaline phosphatase)and hepatic histology returned to normal,independently of their previous therapy.Viable hepatocytes were identified within splenic parenchyma(in group A on d 2)and both in the native liver and the fatty tissue of abdominal wall(in group B on d 5). CONCLUSION:A significantly better survival of the intrasplenically transplanted animals has been demonstrated. Intraperitoneal hepatocytes failed to promptly engraft.A different timing between liver injury and intraperitoneal HCTx may give better results and merits further investigation.
基金the projects of National Major Scientific Research Project (2013CBA01806)National Natural Science Foundation of China (Grant Nos. 41271085,41130641)open fund project of State Key Laboratory of Cryospheric Science (SKLCS-OP2013-05)
文摘To evaluate isotopic tracers at natural abundances by providing basic isotope data of the hydrological investigations and assessing the impacts of different factors on the water cycle, a total of 197 water samples were collected from the Laohugou Glacial catchment in the Shule River basin northwestern China during the 2013 ablation seasons and analyzed their H- and O-isotope composition. The results showed that the isotopic composition of precipitation in the Qilianshan Station in the Laohugou Glacial catchment was remarkable variability. Correspondingly, a higher slope of δ180-δD diagram, with an average of 8.74, is obtained based on the precipitation samples collected on the Glacier No.la, mainly attributed to the lower temperature on the glacier surface. Because of percolation and elution, the bottom of the firn the isotopic composition at is nearly steady. The 6180 /altitude gradients for precipitation and melt water were -o.37%o/100 m and -o.34%o/100 m, respectively Exposed to the air and influenced by strong ablation and evaporation, the isotopic values and the 6180 vs 6D diagram of the glacial surface ice show no altitudinal effect, indicating that glacier ice has the similar origins with the firn. The variation of isotopic composition in the melt water, varying from -l0.7‰ to -16.9‰ （8180） and from -61.1%o to -122.1%o （6D） indicates the recharging of snowmelt and glacial ice melt water produced at different altitudes. With a mean value of -13.3‰ for 8180 and -89.7‰ for 8D, the isotopic composition of the stream water is much closer to the melt water, indicating that stream water is mainly recharged by the ablation water. Our results of the stable isotopic compositions in natural water in the Laohugou Glacial catchment indicate the fractionations and the smoothing fluctuations of the stable isotopes during evaporation, infiltration and mixture.
