电影《Never Let Me Go》无论从叙事、节奏、镜头还是配乐都具有非常地道的英伦风格,克制甚至俭省,端丽进而内敛,讲述一个带有大英帝国暮气的人类未来科幻故事。主人公凯西以年轻美丽的容颜和嗓音注视着、叙述着这一切,语调缓慢犹如古...电影《Never Let Me Go》无论从叙事、节奏、镜头还是配乐都具有非常地道的英伦风格,克制甚至俭省,端丽进而内敛,讲述一个带有大英帝国暮气的人类未来科幻故事。主人公凯西以年轻美丽的容颜和嗓音注视着、叙述着这一切,语调缓慢犹如古希腊哲人阿纳克西曼德吐出悲剧箴言。展开更多
目的探讨食管鳞癌组织中microRNA let-7a-3甲基化状态与血浆中类胰岛素样生长因子2(Insulin like growth factor 2,IGF-Ⅱ)表达的相关性。方法采用甲基化特异性PCR法(Methylation specific PCR,qMSP)检测83例食管癌及相对应的癌旁正常...目的探讨食管鳞癌组织中microRNA let-7a-3甲基化状态与血浆中类胰岛素样生长因子2(Insulin like growth factor 2,IGF-Ⅱ)表达的相关性。方法采用甲基化特异性PCR法(Methylation specific PCR,qMSP)检测83例食管癌及相对应的癌旁正常组织中let-7a-3甲基化状态,采用酶联免疫吸附试验(Enzyme linked immunosorbent assay,ELISA)检测血浆中IGF-Ⅱ的表达水平。结果83例食管鳞癌患者癌组织中的microRNA let-7a-3甲基化程度显著高于癌旁正常组织(P<0.001)。83例食管鳞癌患者血浆中IGF-Ⅱ的表达水平与let-7a-3基因的甲基化程度总体上呈正相关,具有统计学意义(r=0.600,P<0.001)。结论microRNA let-7a-3可能通过对下游分子的甲基化调控参与食管鳞癌的发生发展,这对了解食管鳞癌形成的机制具有重要意义,可为食管鳞癌的诊断和预后提供依据。展开更多
电影Never Let Me Go的中文意思是《不要让我走》,由当代英国文坛最受欢迎的日裔作家石黑一雄的同名小说《不要让我走》改编而成。整部电影平淡而朴实,没有什么大的波澜起伏,给人的感觉就是非常地道的英伦风格。与其说电影讲述的是一个...电影Never Let Me Go的中文意思是《不要让我走》,由当代英国文坛最受欢迎的日裔作家石黑一雄的同名小说《不要让我走》改编而成。整部电影平淡而朴实,没有什么大的波澜起伏,给人的感觉就是非常地道的英伦风格。与其说电影讲述的是一个科幻故事,不如说其讲述的是一个虚幻故事,因为克隆技术在当今时代已不能算是科幻,而电影叙述的内容也不可能出现在当今社会的现实生活中。展开更多
The literary background of Kazuo Ishiguro’s novel Never Let Me Go sets in a highly developed scientific and technological world where clones are created by human and“educated”in the Hailsham School for the sake of...The literary background of Kazuo Ishiguro’s novel Never Let Me Go sets in a highly developed scientific and technological world where clones are created by human and“educated”in the Hailsham School for the sake of“donating”their vital organs to human three or four times before death.By depicting miserable fate of clones,the novel conveys deep concerns on the consequences of abusing technology in modern society.Ever since the burgeoning development of life science and artificial intelligence in the 1990s,the trend of posthumanism/transhumanism has emerged.Posthumanism promotes approaches to enhancing human body condition and organism,and extending human lifespan to augment human capacity and well-being by the advancement of technology.In literary field,contemplation on the relationship between human beings and technology has become a major concern of posthumanism.Putting Never Let Me Go in the context of posthumanism,this essay probes into the significant themes of the novel from three aspects:(1)clones’torments in the existential predicaments imposed by human;(2)human’s violation to the principles of bioethics causing the existential predicaments of alienated clones;(3)appealing to human to ruminate on the relationship between scientific technology and ethics.展开更多
With the announcement of the sheep clone Dolly as the breakthrough in the biotechnology in news media around the turn of the twenty-first century, the rising issue of human clones in its development and the controvers...With the announcement of the sheep clone Dolly as the breakthrough in the biotechnology in news media around the turn of the twenty-first century, the rising issue of human clones in its development and the controversially bioethical issues ensued, Kazuo Ishiguro in Never Let Me Go （2005） focuses his attention, in the area of cell therapy, on how human clones, since produced, lead their model lives and face their deaths, in order that his readers may better understand the meanings of life and death, and that they may stay in a far closer relationship with their family and friends than ever. In this essay, I examine, in two worlds, the normals＇ and the clones＇, paralleling each other, the true meanings of being human and their lives through the perspective of Jacques Derrida＇s deconstruction; and I argue that Ishiguro misspeaks to his readers the true meanings of life and death especially through the clones＇ perspective and brings them to his readers＇ hearts further realistically. In Derrida＇s nature-culture structurality of the clones, it is Kathy H. who comes as center into which the other clones come as freeplay in the structurality of the real world, where it is normals who come as center into which clones come as freeplay under the structurality of power in the institutions where the clones＇ culture comes as center into which Miss Emily＇s ruling comes as freeplay by the structurality of authorship where the author comes as center into which the novel comes as freeplay.展开更多
应用型高校交响管乐团已经在目前的普通高校里斯通见惯,因为在小学、中学甚至到高中都在普及音乐素质教育,从全国来看绝大多数小学、中学和高中都有自己的管乐团甚至交响乐团。它的存在不光能增加学校的素质教育质量,同时还对学生的音...应用型高校交响管乐团已经在目前的普通高校里斯通见惯,因为在小学、中学甚至到高中都在普及音乐素质教育,从全国来看绝大多数小学、中学和高中都有自己的管乐团甚至交响乐团。它的存在不光能增加学校的素质教育质量,同时还对学生的音乐培养有一定推进作用。对于应用型高校交响管乐团来说,它不光能演奏进行管乐同时还能演奏电影音乐等的交响性质的作品。它在演奏时虽然没有弦乐的部分,但通过重新配器,用别的旋律性管乐声部代替弦乐,同样也能产生不错的效果。《冰雪奇缘》是一部优秀的动画片,它是在《安徒生童话》的基础上改编而成的,故事讲的是公主安娜和喜爱冒险的山民克里斯托弗以及一只驯鹿、会说话的雪人一起踏上北上冒险的路程,而他们冒险之旅的目的是为了解除安娜的姐姐——冷酷的白雪皇后给她下的——让她的王国陷入永恒寒冬的诅咒。除了冒险之旅外,影片也将为安娜设置一段纠缠于山民克里斯托弗和潇洒王子汉斯之间的三角恋情,但故事将更多集中在安娜和她的姐姐冰雪皇后艾莎之间的关系。该片一经上映就横扫国际票房,据最新数据显示,迪士尼动画电影《冰雪奇缘》目前全球票房已经累积至10.72亿美元（北美3.98亿,海外6.74亿）,已经正式超越皮克斯的《玩具总动员3》（10.63亿美元）,成为电影史上最卖座的动画电影,同时也是迪士尼公司历史上第一部10亿美元级别的动画电影。其中电影插曲《Let it go》更是一炮而红,不仅被世界上许多大牌歌手演唱过,同时还被翻译成不同国家的语言进行演绎,甚至还被译成了交响乐团、交响管乐团和室内乐团的谱子等。它的知名度和被观众喜爱的程度可见一斑。对于交响管乐团来说,如何演绎一首电影音乐作品,不光光是按曲目要求演绎好,同时还要身临其境地感受他的剧情变化,这样才能给听众带来原汁原味的电影音乐作品。展开更多
Kazuo Ishiguro,a renowned Japanese-British writer,is the winner of the Novel Prize for Literature.The novel Never Let Me Go is a science fiction about human cloning and tells the tragic experience of cloning groups.Ka...Kazuo Ishiguro,a renowned Japanese-British writer,is the winner of the Novel Prize for Literature.The novel Never Let Me Go is a science fiction about human cloning and tells the tragic experience of cloning groups.Kathy,a narrator and protagonist in the story,wrote memories of individual clones and collective clones trauma.She not only witnessed various individual traumas of herself and her friends,but also expressed the cultural trauma of the clones through writing.From the perspective of trauma theory,it can be found that traumatic memories can vent and relieve personal pain,construct collective cultural trauma,and then convey individual’s responsibility for the collective disaster.展开更多
Japanese British writer Kazuo Ishiguro is one of the leading writers in contemporary British literature.He has always been committed to creating works with universal significance.Responsibility and destiny are themes ...Japanese British writer Kazuo Ishiguro is one of the leading writers in contemporary British literature.He has always been committed to creating works with universal significance.Responsibility and destiny are themes that run through his works.His novel Never Let Me Go tells a story of a group of clones growing up in the Hailsham,who are given the mission to donate organs at birth.So,there is no doubt that they will inevitably end their lives in the process of donating organs to human beings again and again.The tragic life of clones is determined by the motivation of human to create them.展开更多
The United States and China have signed an agreement to facilitate Chinese group leisure travel to the United States. This agreement provides the necessary framework to permit group leisure travel from China to th... The United States and China have signed an agreement to facilitate Chinese group leisure travel to the United States. This agreement provides the necessary framework to permit group leisure travel from China to the United States. U.S. companies can now enter into business relationships with Chinese travel agencies to organize and market travel packages for group leisure travel to the United States. It also attracts more and more Chinese to go to America, as more and more convenience and comforts are coming up during the travel.……展开更多
With the guidance of Erikson’s identity theory,the article analyzes the clones’identity exploration through role identification in Never Let Me Go.It interprets the clones’puzzlement about their identity,and the pr...With the guidance of Erikson’s identity theory,the article analyzes the clones’identity exploration through role identification in Never Let Me Go.It interprets the clones’puzzlement about their identity,and the process of their identity quest as well as role identification.Through the specific analysis,it is concluded that the clones,represented by Kathy,Tommy and Ruth,have gained self-certainty and social identity by realizing the role identity as a“carer”and a“donor”,in the meantime,they have constructed their identity as social persons with souls like ordinary people.Furthermore,the findings shows that Never Let Me Go is actually a microcosm of human’s quest for identity.Ishiguro aims to express his meditation on human life in the novel:human life is a process of seeking self-identity and social roles,and then fulfilling the obligations of the roles,which confirms Ishiguro’s internationalism that he attempts to convey his contemplation on human existence through his works.展开更多
Gonitis is a common disorder in the middle-aged and the old people, frequently encountered in the acupuncture department. The following is a brief summary of the 37 cases treated in 1997-1999 when I was dispatched as ...Gonitis is a common disorder in the middle-aged and the old people, frequently encountered in the acupuncture department. The following is a brief summary of the 37 cases treated in 1997-1999 when I was dispatched as a member of foreign aid to work in Malta.展开更多
[目的]研究微小RNA let-7a(microRNA let-7a,miR-let-7a)对脂磷壁酸(Lipoteichoic acid,LTA)诱导的炎性奶牛乳腺上皮细胞(MAC-T)的增殖和凋亡作用。[方法]通过实时荧光定量PCR(Quantitative real time polymerase chain reaction,qRT-P...[目的]研究微小RNA let-7a(microRNA let-7a,miR-let-7a)对脂磷壁酸(Lipoteichoic acid,LTA)诱导的炎性奶牛乳腺上皮细胞(MAC-T)的增殖和凋亡作用。[方法]通过实时荧光定量PCR(Quantitative real time polymerase chain reaction,qRT-PCR)、实时细胞分析仪和流式细胞术检测过表达和抑制表达miR-let-7a在炎性MAC-T中的影响。[结果]qRT-PCR检测结果显示,转染miR-let-7a模拟物会使炎性MAC-T细胞中miR-let-7a的表达量极显著上调(P<0.001),转染miR-let-7a抑制剂会使细胞中miR-let-7a的表达量极显著下降(P<0.001);RTCA结果显示,转染miR-let-7a模拟物能明显促进MAC-T细胞增殖,转染miR-let-7a抑制物会使MAC-T细胞的增殖受到抑制;流式细胞术结果显示,转染miR-let-7a模拟物的MAC-T细胞的凋亡率显著降低(P<0.01);而转染miR-let-7a抑制剂则与之相反,MAC-T细胞的凋亡率显著升高(P<0.01)。即过表达miR-let-7a可以促进MAC-T细胞增殖,并降低细胞的凋亡率。而抑制表达miR-let-7a的作用与之相反,抑制表达miR-let-7a使MAC-T细胞的增殖下降,细胞的凋亡率增大。[结论]miR-let-7a是一种抑制炎症调节因子,其过表达可以缓解奶牛乳腺炎,为阐明奶牛乳腺炎的分子调控机制奠定基础。展开更多
文摘电影Never Let Me Go的中文意思是《不要让我走》,由当代英国文坛最受欢迎的日裔作家石黑一雄的同名小说《不要让我走》改编而成。整部电影平淡而朴实,没有什么大的波澜起伏,给人的感觉就是非常地道的英伦风格。与其说电影讲述的是一个科幻故事,不如说其讲述的是一个虚幻故事,因为克隆技术在当今时代已不能算是科幻,而电影叙述的内容也不可能出现在当今社会的现实生活中。
文摘The literary background of Kazuo Ishiguro’s novel Never Let Me Go sets in a highly developed scientific and technological world where clones are created by human and“educated”in the Hailsham School for the sake of“donating”their vital organs to human three or four times before death.By depicting miserable fate of clones,the novel conveys deep concerns on the consequences of abusing technology in modern society.Ever since the burgeoning development of life science and artificial intelligence in the 1990s,the trend of posthumanism/transhumanism has emerged.Posthumanism promotes approaches to enhancing human body condition and organism,and extending human lifespan to augment human capacity and well-being by the advancement of technology.In literary field,contemplation on the relationship between human beings and technology has become a major concern of posthumanism.Putting Never Let Me Go in the context of posthumanism,this essay probes into the significant themes of the novel from three aspects:(1)clones’torments in the existential predicaments imposed by human;(2)human’s violation to the principles of bioethics causing the existential predicaments of alienated clones;(3)appealing to human to ruminate on the relationship between scientific technology and ethics.
文摘With the announcement of the sheep clone Dolly as the breakthrough in the biotechnology in news media around the turn of the twenty-first century, the rising issue of human clones in its development and the controversially bioethical issues ensued, Kazuo Ishiguro in Never Let Me Go （2005） focuses his attention, in the area of cell therapy, on how human clones, since produced, lead their model lives and face their deaths, in order that his readers may better understand the meanings of life and death, and that they may stay in a far closer relationship with their family and friends than ever. In this essay, I examine, in two worlds, the normals＇ and the clones＇, paralleling each other, the true meanings of being human and their lives through the perspective of Jacques Derrida＇s deconstruction; and I argue that Ishiguro misspeaks to his readers the true meanings of life and death especially through the clones＇ perspective and brings them to his readers＇ hearts further realistically. In Derrida＇s nature-culture structurality of the clones, it is Kathy H. who comes as center into which the other clones come as freeplay in the structurality of the real world, where it is normals who come as center into which clones come as freeplay under the structurality of power in the institutions where the clones＇ culture comes as center into which Miss Emily＇s ruling comes as freeplay by the structurality of authorship where the author comes as center into which the novel comes as freeplay.
文摘应用型高校交响管乐团已经在目前的普通高校里斯通见惯,因为在小学、中学甚至到高中都在普及音乐素质教育,从全国来看绝大多数小学、中学和高中都有自己的管乐团甚至交响乐团。它的存在不光能增加学校的素质教育质量,同时还对学生的音乐培养有一定推进作用。对于应用型高校交响管乐团来说,它不光能演奏进行管乐同时还能演奏电影音乐等的交响性质的作品。它在演奏时虽然没有弦乐的部分,但通过重新配器,用别的旋律性管乐声部代替弦乐,同样也能产生不错的效果。《冰雪奇缘》是一部优秀的动画片,它是在《安徒生童话》的基础上改编而成的,故事讲的是公主安娜和喜爱冒险的山民克里斯托弗以及一只驯鹿、会说话的雪人一起踏上北上冒险的路程,而他们冒险之旅的目的是为了解除安娜的姐姐——冷酷的白雪皇后给她下的——让她的王国陷入永恒寒冬的诅咒。除了冒险之旅外,影片也将为安娜设置一段纠缠于山民克里斯托弗和潇洒王子汉斯之间的三角恋情,但故事将更多集中在安娜和她的姐姐冰雪皇后艾莎之间的关系。该片一经上映就横扫国际票房,据最新数据显示,迪士尼动画电影《冰雪奇缘》目前全球票房已经累积至10.72亿美元（北美3.98亿,海外6.74亿）,已经正式超越皮克斯的《玩具总动员3》（10.63亿美元）,成为电影史上最卖座的动画电影,同时也是迪士尼公司历史上第一部10亿美元级别的动画电影。其中电影插曲《Let it go》更是一炮而红,不仅被世界上许多大牌歌手演唱过,同时还被翻译成不同国家的语言进行演绎,甚至还被译成了交响乐团、交响管乐团和室内乐团的谱子等。它的知名度和被观众喜爱的程度可见一斑。对于交响管乐团来说,如何演绎一首电影音乐作品,不光光是按曲目要求演绎好,同时还要身临其境地感受他的剧情变化,这样才能给听众带来原汁原味的电影音乐作品。
文摘Kazuo Ishiguro,a renowned Japanese-British writer,is the winner of the Novel Prize for Literature.The novel Never Let Me Go is a science fiction about human cloning and tells the tragic experience of cloning groups.Kathy,a narrator and protagonist in the story,wrote memories of individual clones and collective clones trauma.She not only witnessed various individual traumas of herself and her friends,but also expressed the cultural trauma of the clones through writing.From the perspective of trauma theory,it can be found that traumatic memories can vent and relieve personal pain,construct collective cultural trauma,and then convey individual’s responsibility for the collective disaster.
文摘Japanese British writer Kazuo Ishiguro is one of the leading writers in contemporary British literature.He has always been committed to creating works with universal significance.Responsibility and destiny are themes that run through his works.His novel Never Let Me Go tells a story of a group of clones growing up in the Hailsham,who are given the mission to donate organs at birth.So,there is no doubt that they will inevitably end their lives in the process of donating organs to human beings again and again.The tragic life of clones is determined by the motivation of human to create them.
文摘 The United States and China have signed an agreement to facilitate Chinese group leisure travel to the United States. This agreement provides the necessary framework to permit group leisure travel from China to the United States. U.S. companies can now enter into business relationships with Chinese travel agencies to organize and market travel packages for group leisure travel to the United States. It also attracts more and more Chinese to go to America, as more and more convenience and comforts are coming up during the travel.……
文摘With the guidance of Erikson’s identity theory,the article analyzes the clones’identity exploration through role identification in Never Let Me Go.It interprets the clones’puzzlement about their identity,and the process of their identity quest as well as role identification.Through the specific analysis,it is concluded that the clones,represented by Kathy,Tommy and Ruth,have gained self-certainty and social identity by realizing the role identity as a“carer”and a“donor”,in the meantime,they have constructed their identity as social persons with souls like ordinary people.Furthermore,the findings shows that Never Let Me Go is actually a microcosm of human’s quest for identity.Ishiguro aims to express his meditation on human life in the novel:human life is a process of seeking self-identity and social roles,and then fulfilling the obligations of the roles,which confirms Ishiguro’s internationalism that he attempts to convey his contemplation on human existence through his works.
文摘Gonitis is a common disorder in the middle-aged and the old people, frequently encountered in the acupuncture department. The following is a brief summary of the 37 cases treated in 1997-1999 when I was dispatched as a member of foreign aid to work in Malta.
文摘[目的]研究微小RNA let-7a(microRNA let-7a,miR-let-7a)对脂磷壁酸(Lipoteichoic acid,LTA)诱导的炎性奶牛乳腺上皮细胞(MAC-T)的增殖和凋亡作用。[方法]通过实时荧光定量PCR(Quantitative real time polymerase chain reaction,qRT-PCR)、实时细胞分析仪和流式细胞术检测过表达和抑制表达miR-let-7a在炎性MAC-T中的影响。[结果]qRT-PCR检测结果显示,转染miR-let-7a模拟物会使炎性MAC-T细胞中miR-let-7a的表达量极显著上调(P<0.001),转染miR-let-7a抑制剂会使细胞中miR-let-7a的表达量极显著下降(P<0.001);RTCA结果显示,转染miR-let-7a模拟物能明显促进MAC-T细胞增殖,转染miR-let-7a抑制物会使MAC-T细胞的增殖受到抑制;流式细胞术结果显示,转染miR-let-7a模拟物的MAC-T细胞的凋亡率显著降低(P<0.01);而转染miR-let-7a抑制剂则与之相反,MAC-T细胞的凋亡率显著升高(P<0.01)。即过表达miR-let-7a可以促进MAC-T细胞增殖,并降低细胞的凋亡率。而抑制表达miR-let-7a的作用与之相反,抑制表达miR-let-7a使MAC-T细胞的增殖下降,细胞的凋亡率增大。[结论]miR-let-7a是一种抑制炎症调节因子,其过表达可以缓解奶牛乳腺炎,为阐明奶牛乳腺炎的分子调控机制奠定基础。
