Two Households——One Family

I was nine when my parents told me they were separating.Even my sister Meghan, then seven and my brother Patrick, then foursensed that something was wrong between our parents. At fisrt, Momand Dad tried not to argue in front of us, but we saw that they hadtalked to each other less-and they got mad ot us for everything.

One Family Under Two Systems

People usually stop working and start preparing for the festival a week before it arrives.Almost every day has its own special job:mak-ing paper-cuts,buying new clothes,cleaning house,and most impor-tant,preparing veritable mountains of food for visiting friends and rela-tives.According to one Chinese wri-ter,Spring Festival is the most im-portant event in Chinese people's lives:Ordinarily they are the most parsimonious of people,reluctant to waste even a cent,but for Spring Festival,they spend money like mil-lionaires,especially on food.

Tanshinone ⅡA improves Alzheimer’s disease via RNA nuclearenriched abundant transcript 1/microRNA-291a-3p/member RAS oncogene family Rab22a axis

BACKGROUND Alzheimer’s disease(AD)is a neurodegenerative condition characterized by oxidative stress and neuroinflammation.Tanshinone ⅡA(Tan-ⅡA),a bioactive compound isolated from Salvia miltiorrhiza plants,has shown potential neuroprotective effects;however,the mechanisms underlying such a function remain unclear.AIM To investigate potential Tan-ⅡA neuroprotective effects in AD and to elucidate their underlying mechanisms.METHODS Hematoxylin and eosin staining was utilized to analyze structural brain tissue morphology.To assess changes in oxidative stress and neuroinflammation,we performed enzyme-linked immunosorbent assay and western blotting.Additionally,the effect of Tan-ⅡA on AD cell models was evaluated in vitro using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.Genetic changes related to the long non-coding RNA(lncRNA)nuclear-enriched abundant transcript 1(NEAT1)/microRNA(miRNA,miR)-291a-3p/member RAS oncogene family Rab22a axis were assessed through reverse transcription quantitative polymerase chain reaction.RESULTS In vivo,Tan-ⅡA treatment improved neuronal morphology and attenuated oxidative stress and neuroinflammation in the brain tissue of AD mice.In vitro experiments showed that Tan-ⅡA dose-dependently ameliorated the amyloid-beta 1-42-induced reduction of neural stem cell viability,apoptosis,oxidative stress,and neuroinflammation.In this process,the lncRNA NEAT1-a potential therapeutic target-is highly expressed in AD mice and downregulated via Tan-ⅡA treatment.Mechanistically,NEAT1 promotes the transcription and translation of Rab22a via miR-291a-3p,which activates nuclear factor kappa-B(NF-κB)signaling,leading to activation of the pro-apoptotic B-cell lymphoma 2-associated X protein and inhibition of the anti-apoptotic B-cell lymphoma 2 protein,which exacerbates AD.Tan-ⅡA intervention effectively blocked this process by inhibiting the NEAT1/miR-291a-3p/Rab22a axis and NF-κB signaling.CONCLUSION This study demonstrates that Tan-ⅡA exerts neuroprotective effects in AD by modulating the NEAT1/miR-291a-3p/Rab22a/NF-κB signaling pathway,serving as a foundation for the development of innovative approaches for AD therapy.

Moving from fear to realization: Family engagement in tuberculosis prevention in children living in tuberculosis Sundanese households in Indonesia

Objective: Children living in tuberculosis households need protection.However,families need to be engaged to prevent tuberculosis transmission to children.This study aimed to analyze the details of "moving from fear to realization," which is one category of a substantive theory of family engagement in tuberculosis prevention in children.Methods: Fourteen family caregivers participated as key informants after their families met the criteria.The participants were recruited at a lung clinic in West Java,Indonesia and followed up at their homes.The data were gathered using in-depth interviews and analyzed following the coding procedures of the Straussian grounded theory.Findings: "Moving from fear to realization" was the main category that emerged from the experiences of the Sundanese families to begin engaging in tuberculosis prevention in children living in tuberculosis households.It was composed of four sub-categories,namely,fear of tuberculosis,seeking information,following God's will,and awareness of tuberculosis transmission risks.Conclusions: This study showed how a family moves from fear to the realization of tuberculosis transmission risks.Its findings are useful for nurses to provide optimum care in order to prevent tuberculosis transmission to children with household contact by providing both education and counseling for families.

Molecular Composition and Evolution of the Chalcone Synthase (CHS) Gene Family in Five Species of Camellia (Theaceae)

The molecular composition and evolution of the chalcone synthase (CHS) gene family from five species in Camellia (Theaceae) are explored in this study. Sixteen CHS exon 2 from four Camellia species were amplified from total DNA by PCR method. Three sequences of the fifth species in Camellia and two sequences of Glycine max as the designated outgroups were obtained from GenBank. Our results indicated that CHS gene family in Camellia was differentiated to three subfamilies (A, B, C) during the evolutionary history with six groups (A1, A2, A3, BI, B2, C). Among them, only group A2 was possessed by all five species in this study. However, the other five groups were detected only in some species of the plants studied. All members of CHS gene family in this study had high sequence similarity, more than 90% among the members in the same subfamily and more than 78% among different subfamilies at nucleotide level., According to the estimated components of amino acids, the function of CHS genes in Camellia had been diverged. The nucleotide substitutions of the different groups were not identical. Based on phylogenetic analyse inferred from sequences of CHS genes and their deduced amino acid sequences, we concluded that the CHS genes with new function in this genus were evolved either by mutations on several important sites or by accumulation of the mutations after the gene duplication. A further analysis showed that the diversification of CHS genes in Camellia still occurred recently, and the evolutionary models were different to some extant among different species. So we assumed that the different evolutionary models resulted from the impacts of variable environmental elements after the events of speciation.

INVARIANT SUBSPACES AND GENERALIZED FUNCTIONAL SEPARABLE SOLUTIONS TO THE TWO-COMPONENT b-FAMILY SYSTEM

Invariant subspace method is exploited to obtain exact solutions of the two- component b-family system. It is shown that the two-component b-family system admits the generalized functional separable solutions. Furthermore, blow up and behavior of those exact solutions are also investigated.

Estimation of the Number of Collapsed Houses Damaged by Typhoon Based on Principal Components Analysis and Support Vector Machine

The evaluation model was established to estimate the number of houses collapsed during typhoon disaster for Zhejiang Province.The factor leading to disaster,the environment fostering disaster and the exposure of buildings were processed by Principal Component Analysis.The key factor was extracted to support input of vector machine model and to build an evaluation model;the historical fitting result kept in line with the fact.In the real evaluation of two typhoons landed in Zhejiang Province in 2008 and 2009,the coincidence of evaluating result and actual value proved the feasibility of this model.

NONEMPTY INTERSECTION THEOREMS AND SYSTEM OF GENERALIZED VECTOR EQUILIBRIUM PROBLEMS IN PRODUCT G-CONVEX SPACES

By using an existence theorems of maximal elements for a family of set-valued mappings in G-convex spaces due to the author, some new nonempty intersection theorems for a family of set-valued mappings were established in noncompact product G-convex spaces. As applications, some equilibrium existence theorems for a system of generalized vector equilibrium problems were proved in noncompact product G-convex spaces. These theorems unify, improve and generalize some important known results in literature.

TWO CULTURES UNITE IN ONE FAMILY

While some deep-rooted antagonism between the Japanese and Chinese cultures lingers,the younger generation seems to be shrugging off politics

Molecular Clone, Expression, and Prediction of Construction and Function to Key Genes of Interleukin Family of Porcine

This research was to clone, express, and analyze the structure and function of major molecules of porcine interleukin family. Genes of porcine interleukin family were cloned by RT-PCR from stimulated porcine PBMC by LPS and PHA, and then expressed in E. coli, and the structure and function of these molecules were predicted by ExPASY. The results showed that genes of IL-4, IL-6, and IL-18 were successfully cloned and expressed. Furthermore, the expression products of recombinant IL-4 and IL-6 both have multiple biological activities. By analyzing these genes with the NCBI/GenBank data, the homologies of the nucleotide acid sequence are 99.25, 99.21, and 100%, respectively, and have great species differences when compared with other animal species. The results of the prediction showed that all these molecules contain several phosphorylation, glycosylation, protein kinase, and signal transduction bonding sites in secondary structure, and all are compact globularity protein in space configuration. These characteristics of structure are the basis for their multiple biological functions. The genes, structure and function of key molecular of porcine interleukin family were successfully cloned, expressed, and analyzed in this paper.

CHIP regulates bone mass by targeting multiple TRAF family members in bone marrow stromal cells

Carboxyl terminus of Hsp70-interacting protein（CHIP or STUB1） is an E3 ligase and regulates the stability of several proteins which are involved in different cellular functions. Our previous studies demonstrated that Chip deficient mice display bone loss phenotype due to increased osteoclast formation through enhancing TRAF6 activity in osteoclasts. In this study we provide novel evidence about the function of CHIP. We found that osteoblast differentiation and bone formation were also decreased in Chip KO mice. In bone marrow stromal（BMS） cells derived from Chip^-/- mice, expression of a panel of osteoblast marker genes was significantly decreased. ALP activity and mineralized bone matrix formation were also reduced in Chip-deficient BMS cells. We also found that in addition to the regulation of TRAF6, CHIP also inhibits TNFα-induced NF-κB signaling through promoting TRAF2 and TRAF5 degradation. Specific deletion of Chip in BMS cells downregulated expression of osteoblast marker genes which could be reversed by the addition of NF-κB inhibitor. These results demonstrate that the osteopenic phenotype observed in Chip^-/- mice was due to the combination of increased osteoclast formation and decreased osteoblast differentiation. Taken together, our findings indicate a significant role of CHIP in bone remodeling.

NONEMPTY INTERSECTION THEOREMS AND SYSTEM OF GENERALIZED VECTOR EQUILIBRIUM PROBLEMS IN PRODUCT G-CONVEX SPACES

By using an existence theorems of maximal elements for a family of set-valued mappings in G-convex spaces due to the author, some new nonempty intersection theorems for a family of set-valued mappings were established in noncompact product G-convex spaces. As applications, some equilibrium existence theorems for a system of generalized vector equilibrium problems were proved in noncompact product G-convex spaces. These theorems unify, improve and generalize some important known results in literature.

A Family of the Exponential Attractors and the Inertial Manifolds for a Class of Generalized Kirchhoff Equations

In this paper, we studied a family of the exponential attractors and the inertial manifolds for a class of generalized Kirchhoff-type equations with strong dissipation term. After making appropriate assumptions for Kirchhoff stress term and nonlinear term, the existence of exponential attractor is obtained by proving the discrete squeezing property of the equation, then according to Hadamard’s graph transformation method, the spectral interval condition is proved to be true, therefore, the existence of a family of the inertial manifolds for the equation is obtained.

Improving Access to Quality Care in Family Planning:WHO’s Four Cornerstones of Evidence-based Guidance

The four cornerstones of guidance in technique service of family planning are established by WHO based on high quality evidences. They have been updated according to the appearing new evidences, and the consensuses were reached by the international experts in this field. The four documents include Medical Eligibility Criteria for Contraceptive Use, Selected Practice Recommendations for Contraceptive Use, Decision-making Tool for Family Planning Clients and Providers and The Global Handbook for Family Planning Providers. The first two documents mainly face to the policymakers and programme managers and were treated as the important references for creating the local guideline. The other two documents were developed for the front-line health-care and family planning providers at different levels, which include plenty of essential technical information to help providers improve their ability in service delivery and counselling. China paid great attention to the introduction and application of WHO guidelines. As soon as the newer editions of these documents were available, the Chinese version would be followed. WHO guidelines have been primarily adapted with the newly issued national guideline, The Clinical Practical Skill Guidelines-Family Planning Part, which was established by China Medical Association. At the same time, the WHO guidelines have been introduced to some of the clinicians and family planning providers at different levels. In the future, more special training courses will be introduced to the township level based on the needs of grass-root providers.

CDH13 Genetic Polymorphisms, Adiponectin and Ischemic Stroke： a Chinese Family-based Sib-pair Study

Objective To understand the relationships between CDH13（T-cadherin） genetic polymorphisms, adiponectin levels and ischemic stroke, and possible interactions between CDH13 polymorphisms and other risk factors. Methods We recruited 342 Chinese ischemic stroke sib pairs. We genotyped rs4783244 and rs7193788 on CDH13 using time-of-flight mass spectrometry genotyping technology and measured total and high-molecular weight（HMW） adiponectin levels. We investigated associations between SNPs and ischemic stroke, and interactions between SNPs and other risk factors using multi-level mixed-effects regression model. Results In individuals without ischemic stroke, CDH13 rs4783244 was associated with total adiponectin levels（per T： Coef =-0.257, P = 0.001）. CDH13 rs7193788 was associated with total adiponectin levels（per A： Coef =-0.221, P = 0.001） and HMW adiponectin levels（per A： Coef =-0.163, P = 0.003）. rs7193788 was significantly associated with ischemic stroke（GA/AA vs. GG： OR = 1.55, 95% CI： 1.07 to 2.24, P = 0.020） after Bonferroni correction（α = 0.025）. There was an interaction between rs7193788 and diabetes（P = 0.036）. Compared to diabetes-free individuals with rs7193788 GG genotype, diabetes patients with rs7193788 GA/AA genotypes had higher risks for ischemic stroke（OR = 2.64, 95% CI： 1.58-4.40, P 〈 0.001）. Conclusion CDH13 genetic polymorphisms are associated with adiponectin levels and ischemic stroke. An interaction is found between CDH13 SNP and diabetes for ischemic stroke.

Prevalence of Induced Abortion among Female Students in Selected Tertiary Learning Institutions in Gaborone City, Botswana

Induced abortion signifies that pregnancy has been tempered with. Abortion is the removal of an embryo or fetus weighing less than 500 grams from its mother. It can either be spontaneous (unprompted) or induced. Abortion remains one of the leading causes of maternal deaths in developing countries with induced abortion being the key cause. In 2014 from January to August, 9 abortion related deaths occurred in Botswana. There are several reasons female students in tertiary institutions resort to seeking induced abortion which include the socio-demographics like age and socio-economic factors like financial instability. Parental fears, unintended pregnancy and pressure from the partner also force females to terminate pregnancy. Induced abortion has claimed many women’s lives especially in developing countries with very restrictive abortion laws like Botswana as women do not access safe abortion care services. The study intends to establish the determinants of induced abortion among female students in selected tertiary learning institutions in Gaborone City, Botswana. It concentrated primarily on determining the prevalence of induced abortion. This was a descriptive cross-sectional study using both quantitative and qualitative data collection techniques. Systematic random sampling technique was used to come up with 278 participants. Prior to commencement of data collection, all ethical and logistical prerequisites were satisfied. Informed consent was solicited from all the study participants and the purpose of the study was fully explained. A self-administered questionnaire was used to collect data. Microsoft Excel spread sheet was used to code, clean, and enter the data, which was then exported for analysis to the statistical package for social sciences version 24. Frequency distribution tables, percentages graphs, and pie charts were used to illustrate the descriptive information. The study revealed that prevalence of induced abortion among female students in Gaborone City is 7.9%. They even suggested that induced abortion be liberalized. Age was the only socio-demographic factor associated with induced abortion with p = 0.047 using Chi Square test χ2 = 7.609, df = 3. The study findings concluded that female students resort to induced abortion as a result of pressure from both parents and male partners especially when the pregnancy is unintended. The study recommends that the abortion law in Botswana be made liberal to ease access to safe abortion care services. It also recommends that contraceptive commodities should consistently be available in public health facilities.

The Family of Exponential Attractors and Inertial Manifolds for a Generalized Nonlinear Kirchhoff Equations

In this paper, we study the long-time behavior of a class of generalized nonlinear Kichhoff equation under the condition of n dimension. Firstly, the Lipschitz property and squeezing property of the nonlinear semigroup related to the initial-boundary value problem are proved, and then the existence of its exponential attractor is obtained. By extending the space E0 to Ek, a family of the exponential attractors of the initial-boundary value problem is obtained. In the second part, we consider the long-time behavior for a system of generalized Kirchhoff type with strong damping terms. Using the Hadamard graph transformation method, we obtain the existence of a family of the inertial manifolds while such equations satisfy the spectrum interval condition.

Weak influence of natural vegetation in urban green spaces compared to agricultural ecosystems on House Martin populations:Insights from nationwide citizen science data in the Czech Republic

The House Martin(Delichon urbicum)is a common farmland bird species in the European landscape,yet its population numbers are currently in decline.However,it is not yet sufficiently explained why this long-term decline occurs.To fill this gap in our knowledge,we investigated how land cover composition affects the abundance of House Martins on the landscape scale by using nationwide citizen science data.Utilizing a generalised linear mixed-effect model(GLMM),we evaluated 12,094 records from the Czech Republic spanning 2009-2017.Our analysis underscores the significance of land cover type in shaping House Martin abundance.More specifically,our results indicate that within agricultural land covers“naturally managed arable lands”exhibited significant positive effect,while forests,orchards,and vineyards were deemed less favourable for House Martin populations.Within urban land covers,we found a clear distinction in the impact on House Martin populations,with a positive effect observed in urban infrastructure,development areas,and post-industrial sites(i.e.,UrbanAreas),while an indifferent impact was noted within urban green spaces and landscaped areas(i.e.,GreenUrban).Notably,our findings suggest that the simple spatial,age,and species structure typical of forests in Europe,and similarly,the uniform structure of parks and gardens,may be responsible for the decline in the abundance of the House Martin.We advocate for the preservation or enhancement of urban greenery,expansion of natural vegetation in rural areas and adoption of ecological management practices in orchards and vineyards to mitigate further declines in House Martin populations.

Microstructure and properties of 35 kg large aluminum alloy flywheel housing components formed by squeeze casting with local pressure compensation

The squeeze casting method with local pressure compensation was proposed to form a flywheel housing component with a weight of 35 kg.The numerical simulation,microstructure observation and phase characterization were performed,and the influence of local pressure compensation on feeding of thick-wall position,microstructure and mechanical properties of the formed components were discussed.Results show that the molten metal keeps a good fluidity and the filling is complete during the filling process.Although the solidification at thick-wall positions of the mounting ports is slow,the local pressure compensation effectively realizes the local forced feeding,significantly eliminating the shrinkage cavity defects.In the microstructure of AlSi9Mg alloy,α-Al primarily consists of fragmented dendrites and rosette grains,while eutectic Si predominantly comprises needles and short rods.The impact of local pressure compensation on strength is relatively minimal,yet its influence on elongation is considerable.Following local pressure compensation,the average elongation at the compensated areas is 9.18%,which represents a 44.90%higher than that before compensation.The average tensile strength is 209.1 MPa,and the average yield strength is 100.6 MPa.The local pressure compensation can significantly reduce or even eliminate the internal defects in the 35 kg large-weight components formed by squeeze casting.

A Family of Exponential Attractors and Inertial Manifolds for a Class of Higher Order Kirchhoff Equations

In this paper, the global dynamics of a class of higher order nonlinear Kirchhoff equations under n-dimensional conditions is studied. Firstly, the Lipschitz property and squeezing property of the nonlinear semigroup associated with the initial boundary value problem are proved, and the existence of a family of exponential attractors is obtained. Then, by constructing the corresponding graph norm, the condition of a spectral interval is established when N is sufficiently large. Finally, the existence of the family of inertial manifolds is obtained.