Eight Zhes Decoction ameliorates the lipid dysfunction of nonalcoholic fatty liver disease using integrated lipidomics, network pharmacology and pharmacokinetics

Nonalcoholic fatty liver disease(NAFLD)has developed into the most common chronic liver disease and can lead to liver cancer.Our laboratory previously developed a novel prescription for NAFLD,“Eight Zhes Decoction”(EZD),which has shown good curative effects in clinical practice.However,the pharmacodynamic material basis and mechanism have not yet been revealed.A strategy integrating lipidomics,network pharmacology and pharmacokinetics was used to reveal the active components and mechanisms of EZD against NAFLD.The histopathological results showed that EZD attenuated the degrees of collagen deposition and steatosis in the livers of nonalcoholic steatofibrosis model mice.Furthermore,glycerophospholipid metabolism,arachidonic acid metabolism,glycerolipid metabolism and linoleic acid metabolism with phospholipase A2 group IVA(PLA2G4A)and cytochrome P450 as the core targets and 12,13-cis-epoxyoctadecenoic acid,12(S)-hydroxyeicosatetraenoic acid,leukotriene B4,prostaglandin E2,phosphatidylcholines(PCs)and triacylglycerols(TGs)as the main lipids were found to be involved in the treatment of NAFLD by EZD.Importantly,naringenin,artemetin,canadine,and bicuculline were identified as the active ingredients of EZD against NAFLD;in particular,naringenin reduces PC consumption by inhibiting the expression of PLA2G4A and thus promotes sufficient synthesis of very-low-density lipoprotein to transport excess TGs in the liver.This research provides valuable data and theoretical support for the application of EZD against NAFLD.

西宁方言的“着”和“者”

“着[t■■]”和“者[t■■]”是西宁方言中语法功能多样且使用频率很高的两个虚词,二者读音相似且用法上也有交叉。作为动态助词,“着”主要用于持续体,兼表完成体和先行体;而“者”则用于进行体和持续体。作为语气词,二者都出现在句尾,但“着”表祈使语气,而“者”则表肯定和确认。同时,“着”作为处所介词、方式标记和结构助词的用法都是“者”所不具备的。就“着”和“者”的来源来说,表附着义的动词“着”在魏晋时有了方位介词的用法,在唐代语法化为完成体标记,并进一步虚化为结构助词;六朝时“着”还可表持续体,唐代发展为进行体标记,受蒙古语副动词影响,元代“着”产生了方式标记的用法;祈使语气词“着”是由持续体标记演变而来,在此基础上发展为先行体标记。“者”的语气词用法当是由上古的指代词“者”虚化而来,进行体和持续体标记的“者”则是语言接触的产物,是用语气词“者”对译蒙古语动词后的附加成分产生。

汉语助词“着(zhe)”的种种译法

正在走着。/步。走着去学校。/学校。门开着。/開。

从翻译实践看“シテイル”和“着(zhe)”的不对应

"シテイル"作为持续体被列入日语的时体体系之中,自然让人联想到它和表示汉语持续体的符号"着"之间是否完全对应。鉴于《挪威的森林》日文版中含"シテイル"的句子十分丰富,作者选定该原著及其中译本(林少华版)作为展开分析的语料数据库,从对比语言学的角度来考察"シテイル"和"着"的对应情况,统计结果显示两者在绝大多数情况下并不对应,进而从中日两种语言在词汇、句法上的差别来分析不对应的根本原因。

大黄zhe虫胶囊联合力克敏治疗慢性乙肝36例

我院自2002年6月-2003年6月应用大黄zhe虫胶囊联合力克敏注射液治疗慢性乙型肝炎36例，取得良好效果，现报道如下：

现代汉语“着(·zhe)”的研究综述

＂着（·zhe）＂在现代汉语中的使用频率很高,但是对其语法意义,先哲时贤却众说纷纭,令人莫衷一是。例如表动作进行,表状态持续,表惯性,表情状等等。这种情况对于汉语信息输入和对外汉语教学都是不利的。有鉴于此,本文拟全面考察前人对＂着（·zhe）＂的语法分布和语法意义的研究,争取得出前人相关研究的成败,为今后关于＂着（·zhe）＂的研究提供一定的借鉴。

Plasma pharmacological study on Da Huang Zhe Chong capsule on platelet aggregation of normal person

Objective To develop a plasma pharmacological method evaluates the effect of Da Huang Zhe Chong capsule on platelet aggregation and its mechanism, which is a representative Traditional Chinese Medicine Patent Prescription Promoting blood circulation by removing blood stasis. Methods Platelets specimens from healthy volunteers made serum and plasma with medicine, while platelet PRP were separated, which were divided into 8groups,i.e. auto-serum, allo-serum, serum with Da Huang Zhe Chong capsule , serum with aspirin, auto-plasma, plasma with Da Huang Zhe Chong capsule, plasma with aspirin, every group added to serum and plasma to hatch. After ADP and adrenalin were added into the specimens and hatched, the effects of specimens on platelet aggregation were observed. Results After ADP adrenalin were added, all the serum groups did not present platelet aggregation,while all the plasma group presented platelet aggregation. P1, P5, Pmax, t and TM have no significant difference (P＞0. 05) between auto-plasma group and allo-plasma group induced by ADP and adrenalin. P1, P5, t and Pmax have significant differences (P＜0. 01) and TM decreased significantly (P＜0. 05) comparing plasma group with Da Huang Zhe Chong capsule and plasma group of aspirin to allo-plasma group. P1, t and Pmax have significant difference (P＜0.05), and P5 and TM are simulate comparing plasma group with Da Huang Zhe Chong capsule to plasma group of aspirin. P1, P5, t and Pmax have significant differences (P＜0. 01), P1, TM have also significant(P＜0. 05), comparing plasma group of Da Huang Zhe Chong capsule with plasma of aspirin to allo-plasma group induced by adrenalin. P1 ,P5 and Pmax have significant differences (P＜0.05), and t and Pmax are simulate comparing plasma group with Da Huang Zhe Chong capsule with plasma group of aspirin. Conclusion The serum pharmacological study is inappropriate to study platelet aggregation in vitro. The plasma pharmacological study is inappropriate to study platelet aggregation in vitro. The plasma pharmacological study could reflect the pharmacological effect produced in vivo. Da Huang Zhe Chong capsule has better anti artery thrombosis effect than aspirin, and it is an ideal medicine for anti artery thrombosis.

Developing International Economic and Technological Cooperation in Zhe Jiang

Zhejiang is a coastal province with advanced economy,solid foundation of industry and agriculture and all-comprehensive industry sectors,and with lignht industry andtextile as the mainstay Its popularized education,advancedscience and technology and ample supply of high-quality labourforce benefit itselt involving in foreign project contracting,labour export and overseas investment.Since 1979,Zhejianghas accepted more than ten foreign projects worth US$300million and supplied labour service to 40 countries。

Impact Assessment of Diesel Oil on the Zhe Oyster （Crassostrea plicatula） Using RAPD Analysis

The lethal and sublethal effects of oils on aquatic organisms have been widely investigated, but the potential molecular impacts of oils on aquatic organisms are remaining unclear now. In order to realize the effects of diesel oil on the Zhe oyster, the RAPD （random amplified polymorphic DNA） technique was used. RAPD is a useful assay procedure for the detection of genotoxin-induced DNA damage and mutations. In the present study, the Zhe oysters were exposed to diesel oil at different concentrations and for different exposure periods. The results showed that the DNA band change in RAPD profiles of oysters following diesel oil treatment included loss of normal DNA bands, the appearance of new DNA bands and variations in DNA intensity compared to oysters not exposed to diesel oil. The effects of changes to GTS （genome template stability） were time- and concentration-dependent, the GTS of 10 mg/L was 82.46%, 80.70% and 63.15% in the 8, 16 and 32 days, the GTS of 20 mg/L was 75.44%, 71.93% and 56.14% in the 8, 16 and 32 days, the GTS of 40 mg/L was 73.68%, 70.18% and 56.14% in the 8, 16 and 32 days, respectively. The DNA polymorphisms detected by RAPD analysis could be used as a useful biomarker assay for the detection of genotoxic effects in diesel oil pollution on the oysters, and may be useful for environmental contamination risk assessment.

大黄zhe虫丸外敷治疗耳鸣耳聋

我们用经方“大黄zhe虫丸”外敷治疗一例瘀血阻滞型耳鸣耳聋，效果满意介绍为下。

大黄Zhe虫丸合卵磷脂抗肝纤维化的临床研究

目的：观察中药大黄Zhe虫丸合用卵磷脂对慢性乙型肝炎肝纤维化的疗效。方法：选择慢性乙肝纤维化指标异常的患者90例，随机分成治疗组及对照组各45例，治疗组口服大黄Zhe虫丸合卵磷脂；对照组口服凯西莱，观察治疗后肝纤维化指标的变化。结果：治疗组HA、LN、PCⅢ、IV－C、ALT、AST有明显降低，而对照组各项指标下降不明显（P＜0．01、P＜0．05）。结论：大黄Zhe虫丸合卵磷脂的抗纤维化作用优于凯西莱。

Zhe9248—a new scented rice line cames out in Zhejiang

This novel rice line is developed recently’by a research team led by Associate Prof QIU Baiqin, Crop Breeding and Cultivation Institute, Zhejiang Academy of Agricultural Sciences. Zhe 9248 is of high resistance to blast, early mature and of higher yield. Being soft, smooth, and glossy, the cooked rice of "Zhe 9248" has excellent eating quality. The kernels of "Zhe 9248" are hardened to such a degree that they could be capa-

大黄蟅虫丸抗大鼠免疫性肝纤维化研究

目的：研究大黄虫丸防治肝纤维化的作用。方法：运用大鼠免疫性肝纤维化模型，观察大黄虫丸对肝组织病理学、肝羟脯氨酸（ＨＹＰ）含量的影响。结果：大黄虫丸预防和治疗试验肝纤维化率分别为７２．７％和７１．２％，而模型组和秋水仙碱组肝纤维化率为９２．３％～１００％，尤其是大黄虫丸治疗试验组肝ＨＹＰ含量下降显著，与模型组比较Ｐ＜０．０５。结论：大黄虫丸有一定的抗肝纤维化作用。

大黄虫丸对大鼠肝星状细胞表达TGF-β_1的影响

目的 :探讨大黄虫丸对大鼠肝星状细胞表达转化生长因子β1(TGF β1)的影响。方法 :用CCl4复合因素致大鼠肝纤维化模型 ,同时分别给予 3种剂量大黄虫丸治疗 ,免疫组化方法观察TGF β1、α 平滑肌肌动蛋白 (α SMA)的合成表达及纤维化程度分级。结果 :经给予大黄虫丸干预治疗常规剂量组大鼠TGF β1、α SMA表达与模型组比较差异无显著性 (P >0 0 5 ) ,但双倍剂量组和三倍剂量组TGF β1、α SMA在汇管区及不连续纤维间隔的表达明显减弱 (P <0 0 5 ,P <0 0 1)。结论 :大黄虫丸较大剂量能抑制肝星状细胞增殖和分泌TGF β1,减少胶原生成 ,从而减弱肝星状细胞的自分泌放大效应 ,这可能是大黄虫丸抗肝纤维化作用的主要机制之一。