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新型“三明治”结构DNA电化学传感器对急性早幼粒细胞白血病相关融合基因的检测 被引量:2
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作者 陈婧 陈敬华 +4 位作者 陈元仲 林启凰 罗红斌 李光文 林新华 《分析测试学报》 CAS CSCD 北大核心 2009年第12期1357-1361,共5页
基于急性早幼粒细胞白血病(APL)中PML/RARa融合基因的碱基序列,设计了新型的锁核酸(LNA)修饰寡核苷酸作为捕获探针和信号探针,研究出一种基于“三明治”传感模式的电化学生物传感器对PML/RARa融合相关基因进行检测。靶序列分别... 基于急性早幼粒细胞白血病(APL)中PML/RARa融合基因的碱基序列,设计了新型的锁核酸(LNA)修饰寡核苷酸作为捕获探针和信号探针,研究出一种基于“三明治”传感模式的电化学生物传感器对PML/RARa融合相关基因进行检测。靶序列分别与捕获探针和信号探针杂交后形成“三明治”结构。将修饰电极置于含有底物3,3’,5,5'-四甲基联苯胺(TMB)和过氧化氢的测定溶液中,用计时电流法检测靶序列。结果表明,该传感器可定量识别和检测溶液中人工合成的短链APLPML/RARa融合基因片段。经过条件优化,杂交前后电流值与靶标链浓度在1.0×10^-12 -2.5×10^-11mol/L范围内呈良好的线性关系,检出限为8.5×10^-13mol/L。该方法简单、特异性好,有望用于实际样品的检测。 展开更多
关键词 '三明治'结构 DNA电化学探针 PML/RARΑ融合基因 计时电流法 辣根过氧化物酶
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In-situ Micro-CT analysis of deformation behavior in sandwich-structured meta-stable beta Ti−35Nb alloy
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作者 Yu-jing LIU Zi-lin ZHANG +4 位作者 Jin-cheng WANG Xiang WU Xiao-chun LIU Wei-ying HUANG Lai-chang ZHANG 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS 2024年第8期2552-2562,共11页
Beta Ti−35Nb sandwich-structured composites with various reinforcing layers were designed and produced using additive manufacturing(AM)to achieve a balance between light weight and high strength.The impact of reinforc... Beta Ti−35Nb sandwich-structured composites with various reinforcing layers were designed and produced using additive manufacturing(AM)to achieve a balance between light weight and high strength.The impact of reinforcing layers on the compressive deformation behavior of porous composites was investigated through micro-computed tomography(Micro-CT)and finite element method(FEM)analyses.The results indicate that the addition of reinforcement layers to sandwich structures can significantly enhance the compressive yield strength and energy absorption capacity of porous metal structures;Micro-CT in-situ observation shows that the strain of the porous structure without the reinforcing layer is concentrated in the middle region,while the strain of the porous structure with the reinforcing layer is uniformly distributed;FEM analysis reveals that the reinforcing layers can alter stress distribution and reduce stress concentration,thereby promoting uniform deformation of the porous structure.The addition of reinforcing layer increases the compressive yield strength of sandwich-structured composite materials by 124%under the condition of limited reduction of porosity,and the yield strength increases from 4.6 to 10.3 MPa. 展开更多
关键词 beta titanium alloy sandwich-structured composite in-situ micro-computed tomography finite element modeling compressive behavior
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