树莓‘沙尼’组培技术

应用树莓（Rubus ideaus L.）的优良品种‘沙尼’（‘Shawnee’）进行组织培养技术的探索和研究,以期为树莓的大量繁育和推广提供技术支撑。结果表明：最佳外植体的选择为带芽茎段,最佳的取材时间为5月份,萌发率可达100%。最佳灭菌体系的流程为75%酒精浸泡30 s后无菌水冲洗3~4次,然后再以2%Na Cl O灭菌20 min后无菌水冲洗3次,污染率达到0%。‘沙尼’的最佳初代培养基为MS＋6-BA 1.0 mg·L-1＋IAA 0.5 mg·L-1;最佳继代培养基为MS＋6-BA 0.5 mg·L-1＋NAA 0.05 mg·L-1,增殖系数为3.49;最佳生根培养基为1/2MS＋IBA 1.0 mg·L-1,生根率为100%,植株可平均生出2 cm的健壮根系10条。

Autoantibodies and hepatitis C virus genotypes in chronic hepatitis C patients in Estonia

AIM: To determine the prevalence of several autoantibodies in chronic hepatitis C patients, and to find out whether the pattern of autoantibodies was associated with hepatitis C virus (HCV) genotypes. METHODS: Sera from 90 consecutive patients with chronic hepatitis C were investigated on the presence of anti-nuclear (ANA), anti-mitochondrial (AMA), anti-smooth muscle (SMA), anti-liver-kidney microsomal type 1 (LKMA1), anti-parietal cell (PCA), anti-thyroid microsomal (TMA), and anti-reticulin (ARA) autoantibodies. The autoantibodies were identified by indirect immunofluorescence. HCV genotypes were determined by a restriction fragment length polymorphism analysis of the amplified 5' noncoding genome region. RESULTS: Forty-six (51.1%) patients were positive for at least one autoantibody. Various antibodies were presented as follows: ANA in 13 (14.4%) patients, SMA in 39 (43.3%), TMA in 2 (2.2%), and ARA in 1 (1.1%) patients. In 9 cases, sera were positive for two autoantibodies (ANA and SMA). AMA, PCA and LKMAI were not detected in the observed sera. HCV genotypes were distributed as follows: 1b in 66 (73.3%) patients, 3a in 18 (20.0%), and 2a in 6 (6.7%) patients. CONCLUSION: A high prevalence of ANA and SMA can be found in chronic hepatitis C patients. Autoantibodies are present at low titre (1:10) in most of the cases. Distribution of the autoantibodies show no differences in the sex groups and between patients infected with different HCV genotypes.

Lactase non-persistence and milk consumption in Estonia

AIM： To define the frequency of the cfr-13910 variant associated with lactase persistence/non-persistence trait and to analyze the milk consumption of lactase non- persistent subjects in Estonia. METHODS： We genotyped 355 Estonians by polymerase chain reaction and direct sequencing, Milk consumption was analyzed by a questionnaire, specially developed to analyze milk consumption and abdominal complaints. RESULTS： The frequency of the genotype of the C/ C-13910 （lactase non-persistence） was found to be 24.8% in native Estonians. No other single nucleotide polymorphisms covering the region of 400 bp adjacent to the C/T-13910 variant were found. Lactase non- persistence subjects were found to consume less milk than lactase persistence subjects. CONCLUSION： The frequency of lactase non- persistence defined by the C/C-13910 genotype confirms the results of the previous studies based on indirect methods of determining hypolactasia, Milk consumption of lactase non-persistence subjects is consistent with previously reported figures of adult-type hypolactasia in Estonia, However, lactase non-persistence does not prevent the intake of milk in many adults.

Change of Management Values in Estonian Business Life in 2007-2009

The paper deals with the change of management values in Estonian business life in the year of the rapid growth （2007） as compared with the year of hard decline （2009）. The hypothesis is set that company managers do not have permanent values but they change accordingly to the change in economic conditions： in economical welfare situations business-managers more eagerly take larger responsibilities on ethical and philantrophical stages but in crisis only the lower stages （economical and legal ones） could be detained. In the paper, we firstly observe if values change in general and secondly we study if our pre-stated hypothesis holds. As the research method we use the method of critical incidents

Opportunities of manufacture under global economic crisis： The case of Estonia

Because of the smallness of Estonia, its main source of economic growth is export, i.e. successful sales in the world market. It is necessary to shift from branches, activities and value chains where it is difficult to increase value added, to more profitable and promising ones. The present economic situation provides for this an exceptionally good chance. All kinds of development, international relocation of production and reallocation of economic power in the world are gathering speed. It is necessary to rapidly find an opportunity in the ＂declining＂ market, a profitable niche. Manufacturing entrepreneurs and executive managers have for several years already been complaining of shortage of workforce that hinders them carrying out new business plans, increasing production and exports. Increased supply of workforce dismissed from construction is an opportunity for the forced development of the manufacturing export sector and sharp increase in exports.

Controlling and Corporate Social Responsibility： A Comparative Study of German and Estonian Controlling

This paper focuses on the functions of controlling in Estonia and Germany, The analysis based on the study carried out in Estonian enterprises in 2008. The study was conducted by the Estonian work group of the International Controller Association ICV （internationaler controller verein）. The methodology and controlling questionnaire were the same, which were used by the German work group of ICV in 2006. The mentioned questionnaire was also worked out in Germany by Otto Beisheim School of Management. The authors compared the results of both studies Cultural differences and their impact on the tasks of controllers were also scrutinized. The results of the research indicate that the tasks of Estonian controllers are strictly fixed with procedure documents, which leaves very lithe room lbr independence and initiative. Usually, they are not involved in the strategic planning and goal setting. The final conclusion is that the sustainability function of controlling is much more on the background compared to other functions of controlling, but in the future the role of this function is expected to increase

Management Accounting Problems and Perspectives in the Local Government of Estonia： An Analysis From the Viewpoint of Parsons＇ Paradigm

This case study focuses on the current and future needs of the management accounting within the Tallinn city government and their potential relationships with the sustainability reporting. This analysis is based on the study carried out within the city organizations in 2011. The questionnaire drawn up by the authors includes several questions about the current management accounting tools of the city and their development and support needs. The authors also add some questions related to the sustainability reporting, which is obligatory for the city as one of the members of the Aalborg Charter. The quantitative methodology was used. The authors＇ conclusion is that the level of the managerial accountability of the Estonian municipality is increasing and its sole economic focus is changing to a broader perspective. According to the findings of the study, the authors argue that both management accounting principles and sustainability principles are coming closer together. By using Talcott Parsons＇ adaptation, goal attainment, integration, and latency （AGIL） paradigm, the authors point out four critical components for implementing the sustainability reporting into a management accounting system. The results of this study highlight the role of values in the management accounting development, which is in harmony with the AGIL paradigm.

The Latvian Road to the Euro

The article presents the situation in Latvia ahead of the country＇s adoption of the euro by offering an analysis of the extent to which the country is prepared for the move and of general economic difficulties as well as indicating possible threats to its future development. The article talks about difficulties caused by the great unemployment connected with the specific economy of the country, political problems of the post-socialistic multinational country and steps done towards the fulfilling of all the convergence criteria. Its aim is to present the moments around the change of the currency which is done by the material＇s analysis because there are not enough statistical data for the mathematical analysis. It can be concluded that the adoption of the euro will probably be beneficial in the long run for Latvia＇s economy. The conclusions may be done by comparison with the situation of Estonia and its foreign trade with Poland which is shown in the article because there are a lot of similarities between the two Baltic countries.

Analysis of the Estonian Forest Conservation Area Network

Estonian Forestry Development Programme set in 2003 ambitious goal that area of strictly protected forests should increase at least to 10% from total forest area in Estonia before 2010 by expanding the area of old-growth under protection, improving the representativeness of conservation areas and establishment of large conservation sites. Inventory of forests was carried out on existing and possible new conservation areas within the Estonian Forest Conservation Area Network project （EFCAN） in 1999-2001. EFCAN project had remarkable results. The share of strictly protected forests has increased to 7.8% of total forest area by 2009 and the network of conservation areas is quite well covering all forest ecosystems in Estonia. Several selected areas are still not protected for different reasons. These areas should still be considered for protection as the areas may lose their conservation value. Several forest types （meso-eutrophic, eutrophic boreo-nemoral and eutrophic paludifying forests） should have larger area for conservation and large disturbance areas （wind damage, forest fire） should be also included into the network.

Estonian Taxation System and Innovation

The economic crisis of the last three years, which has hit the world, especially the Baltic States together with their ultraliberal way of economy, has forced to look for solutions to overcome the depression. A low level of taxation, the policy of＂thin state policy＂ and a small share of public sector have influenced the macroeconomics of Estonia since its re-independence. The indirect taxes, especially the consumption taxes, are dominating in Estonian taxation system. The increase of tax burden a little more than 2% in 2009 through the increase of value added tax （VAT） and excises, and the pruning of income taxation benefits did not enlarge state budget in the same amount. The pruning of budget did not just decrease the internal market of the state very rapidly, but also decrease the incomes of the future periods, due to a big dominant of consumption taxes. The economic depression, which began in 2008, has demonstrated a week orientation of Estonian economy, threaten its taxation system on innovation. The amount of investments has essentially decreased than the decrease of GDP and state budget.

Ideology and Poetics in the Estonian Translations of Nikolai Leskov

This paper brings up the issue of a systemic study of the translation of Russian classics into Estonian during the Soviet period. These translations are often considered to be only the result of the ＂Sovietization＂ of Estonian culture, but such approach is a simplification. Estonian translations of the Russian classics in the Soviet period, undoubtedly, depended on ideological conditions, but, it is argued here, were to a great extent determined by the mechanisms of cultural self-regulation and displayed continuity with translation strategies that had been established before the occupation, in the 1920s-1930s. In the independent Estonian Republic, a remarkable tradition of the translations of Russian classics existed thanks to the activity of A. H. Tammsaare, F. Tuglas, A. Oras, H. Talvik, B. Alver et al. Estonian translators devoted the particular attention to the text＇s audience--the Estonian readers. The paper explains why in the mid 19S0s Leskov, an ambivalent and diffficult-to-translate writer, was chosen by O. Samma and F. Kylli for the first presentation to the Estonian readers.

The Impact of the Economic Crisis on the Production Function of Estonian Banking

Econometric models have not been widely used for thorough analysis of the statistics published by banks. The paper presents the results of analyses of Estonian Banking, made by the authors during the last seven years. Earlier results have been published in a number of collections of research articles and have been reported on international conferences worldwide （in Mexico, South Africa, Egypt, Poland, Check Republic, Chile, China, and USA, etc.）. From econometric models, the Cobb-Douglas production function with income-earning assets, equity, liabilities and fixed assets as inputs was selected. The time period chosen is from the first quarter of 1995 to the fourth quarter of 2008 that is a total of 56 periods. The balance sheets and income statements published quarterly by the banks were used as the empirical base of analysis.

Methodology for Defining of Eligible Capacity for Wood Fuel Based Cogeneration Plants in Small Towns in Estonia

There is a high potential for small-scale and medium scale wood-fired cogeneration in Estonia. The purpose of this research is to define the eligible capacity for wood fuel based cogeneration plant operating on the base of district heating systems in small towns. Results were checked and approbated by economical and environmental factors. Two optimal sizing methods were used maximizing of amount of heat year-round while working at full installed capacity and maximizing of amount of heat year-round while working with partial loads. Results of defining optimal capacities for wood-fired cogeneration plants in small Estonian towns showed that method of maximizing of amount of heat year-round while working with partial loads is more adequate to real situation.

Estonia： The Prospects of Nuclear Energy

This paper details the prospects for a civil nuclear power station in Estonia. Due to its climate change commitments, Estonia needs a new source of electricity provision. There is little academic literature on Estonia, and this research builds upon previous work but is more detailed in its analysis with primary data included in the form of interviews with Estonian energy industry experts. All the conditions that have led to nuclear energy being considered as an option are assessed, along with why it represents the most strategic path for Estonia in terms of electricity provision. Through the interview analysis a framework is established and advanced for the successful initiation of a national nuclear power plant project. This research is therefore of high value for small EU countries who intend to consider nuclear energy as an option in their energy mix or have more advanced plans to develop a nuclear program.

Assessment on Development of Wind Energy in Offshore Regions of Estonia

Up to now legislation of economical usage of Estonian sea shelf is lacking, what breaks development of wind farms. Therefore it is expedient to look the potential of wind resources and prablems to use it. In this paper the potential resource of four characteristic offshore regions in Estonia are assessed. Two different approaches have been used to assess the dynamic quality of the wind power, both of which lead to the same result. The dynamic quality of the load of the grid is also assessed since any consumer will be affected by the total influence of both these dynamic processes. These processes do limit the usage of wind power due to peculiarities of the Estonian electrical grid.

Understanding the influence of microwave on the relative volatility used in the pyrolysis of Indonesia oil sands

In this paper, pyrolysis of Indonesian oil sands （lOS） was investigated by two different heating methods to develop a better understanding of the microwave-assisted pyrolysis. Thermogravimetric analysis was conducted to study the thermal decomposition behaviors of lOS, showing that 550 ℃ might be the pyrolysis final temperature. A explanation of the heat-mass transfer process was presented to demonstrate the influence of mi- crowave-assisted pyrolysis on the liquid product distribution. The heat-mass transfer model was also useful to explain the increase of liquid product yield and heavy component content at the same heating rate by two differ- ent heating methods. Experiments were carried out using a fixed bed reactor with and without the microwave irradiation. The results showed that liquid product yield was increased during microwave induced pyrolysis, while the formation of gas and solid residue was reduced in comparison with the conventional pyrolysis. Moreover, the liquid product characterization by elemental analysis and GC-MS indicated the significant effect on the liquid chemical composition by microwave irradiation. High polarity substances （ε 〉 10 at 25 ℃）, such as oxy- organics were increased, while relatively low polarity substances （ε 〈 2 at 25℃）, such as aliphatic hydrocarbons were decreased, suggesting that microwave enhanced the relative volatility of high polarity substances. The yield improvement and compositional variations in the liquid product promoted by the microwave-assisted pyrolysis deserve the further exploitation in the future,

Global influenza in cold phase of Pacific Decade Oscillation

Based on the study of the cold phase of the Pacific Decade Oscillation, pandemic influenza is related to climate. The relation of low temperature, Pacific Decade Oscillation, strongest earthquake, Influenza, hurricane and E1 Nino is researched in this study. In the cold period of Pacific Decade Oscillation, the strongest earthquake, hurricane with La Ni＇na, Pandemic Influenza with E1 Nino will occur stronger and stronger. From 1950 to 1976, the strongest dust-storm is connected with Pandemic Influenza one by one. So, dust-storm is one of factors to spread pandemic influenza viruses.

The Effect of TV Captions on the Comprehension of Non-Native Saudi Learners of English

This paper investigates the effectiveness of closed captioning in aiding Saudi students who are learning ESL （English as a second language）. Research was carried out in a qualitative manner, and participants were 12 Saudi students pursuing their studies at Indiana University of Pennsylvania, USA （IUP）. Participants in the study were asked to compose a narrative after viewing a 5-minute film segment, both with and without captioning. Their responses were then analyzed, and results indicated that while captions may aid one in comprehension, they also tend to limit one＇s interpretations, reaffirming the nature of written language as an authoritative source of information.

Populism and Political Party Institutionalisation in the Three Baltic States of Estonia, Latvia and Lithuania

The three Baltic states of Estonia, Latvia and Lithuania make for interesting comparative case studies in the rise of contemporary populism in Europe. All three countries share a similar modem history, democratic political system, level of economic development and are in the same western-oriented regional clubs （NATO, European Union, OSCE, European Council and, in the case of Estonia and Latvia, OECD, etc.）. However, they do differ in the extent to which populism has played a role in their national politics. Populism has played a marginal role in Estonian politics, but has long been central to the Latvian party system and influential in Lithuanian politics. This article tackles two key issues in the study of populism. First, it focuses on political party institutionalisation as the key variable in explaining the differing impact of populist political parties across the Baltic states. It examines and explains the higher turnover of political party creation and destruction in Latvia and, to a lesser extent, Lithuania and argues that institutions--and par- ticularly the laws that frame party organisations and participation in elections-- create political opportunities for populist parties. The second part of the paper considers the different types of populist parties to have emerged in the Baltic states in recent decades. While previous studies of populism in the Baltic states （such as Auers and Kasekamp in J Contemp Eur Stud 17（2）：241-254, 2009; Auers and Kasekamp, in： Wodak et al （eds） Right wing populism across/beyond Europe, Bloomsbury Academic Press, London 2013） have explicitly focused on Radical Right Populist parties, this article additionally identifies a number of parties in the region that are not explicitly radical right, but are certainly populist.