[Objective] This study aimed to investigate the browning of T. cuspidata cells in suspension culture and provide the guidance for the cell suspension culture of T. cuspidata. [Method] T. cuspidata callus was used as e...[Objective] This study aimed to investigate the browning of T. cuspidata cells in suspension culture and provide the guidance for the cell suspension culture of T. cuspidata. [Method] T. cuspidata callus was used as experimental materials, to explore the effect of different medium, N/P ratio, pH, shaking speed, illumination time and light intensity and other factors on browning of T. cuspidata cells in suspension culture. [Result] Non-browning callus was transferred to 2MB5 medium （pH 7.0） for illumination culture at 22℃ under light intensity of 1 500 lx with shaking speed of 90 r/min for 24 h. Results showed that the cell browning was significantly inhibited. [Conclusion] This study laid the foundation for cell suspension culture of T. cuspidata and had important significance to the large-scale industrial production of paclitaxel.展开更多
Algae of the order Laminariales represent main seaweed resource of Russia, Most important commercial species formerly belonged to Laminaria Lamoroux. However, taxonomic revision of this genus and resurrection of Sacch...Algae of the order Laminariales represent main seaweed resource of Russia, Most important commercial species formerly belonged to Laminaria Lamoroux. However, taxonomic revision of this genus and resurrection of Saccharina Stackhouse based on genetic studies resulted in transfer of most Laminaria species to Saccharina. At the Russian Pacific coasts Saccharina now includes 10 species, while only three species for sure represent Laminaria： L. longipes Bory de Saint-Vincent, L. solidungula J. Agardh, L. yezoensis Miyabe, their placement within Laminaria is supported by genetic data. All examined Far Eastern species of Saccharina are noticed to possess haptera in the form of rhizoids, whereas members of Laminaria have disk-like holdfasts （L. solidungula, L. yezoensis）, or creeping rhizomes （L. longipes）. Three unstudied genetically endemics from the Sea of Okhotsk： L. appressirhiza Petrov et Vozzhinskaya, L. inclinatorhiza Petrov et Vozzhinskaya, L. multiplicata Petrov et Suchovejeva, are still left within Laminaria, but they have rhizoids, so most probably belong to Saccharina. This morphological criterion is inapplicable to Atlantic Laminaria since the type species L. digitata （Hudson） Lamouroux has rhizoids.展开更多
基金Supported by National Natural Science Foundation of China (31070164)Young Scientists Fund of Dalian (2006J23JH031)~~
文摘[Objective] This study aimed to investigate the browning of T. cuspidata cells in suspension culture and provide the guidance for the cell suspension culture of T. cuspidata. [Method] T. cuspidata callus was used as experimental materials, to explore the effect of different medium, N/P ratio, pH, shaking speed, illumination time and light intensity and other factors on browning of T. cuspidata cells in suspension culture. [Result] Non-browning callus was transferred to 2MB5 medium （pH 7.0） for illumination culture at 22℃ under light intensity of 1 500 lx with shaking speed of 90 r/min for 24 h. Results showed that the cell browning was significantly inhibited. [Conclusion] This study laid the foundation for cell suspension culture of T. cuspidata and had important significance to the large-scale industrial production of paclitaxel.
文摘Algae of the order Laminariales represent main seaweed resource of Russia, Most important commercial species formerly belonged to Laminaria Lamoroux. However, taxonomic revision of this genus and resurrection of Saccharina Stackhouse based on genetic studies resulted in transfer of most Laminaria species to Saccharina. At the Russian Pacific coasts Saccharina now includes 10 species, while only three species for sure represent Laminaria： L. longipes Bory de Saint-Vincent, L. solidungula J. Agardh, L. yezoensis Miyabe, their placement within Laminaria is supported by genetic data. All examined Far Eastern species of Saccharina are noticed to possess haptera in the form of rhizoids, whereas members of Laminaria have disk-like holdfasts （L. solidungula, L. yezoensis）, or creeping rhizomes （L. longipes）. Three unstudied genetically endemics from the Sea of Okhotsk： L. appressirhiza Petrov et Vozzhinskaya, L. inclinatorhiza Petrov et Vozzhinskaya, L. multiplicata Petrov et Suchovejeva, are still left within Laminaria, but they have rhizoids, so most probably belong to Saccharina. This morphological criterion is inapplicable to Atlantic Laminaria since the type species L. digitata （Hudson） Lamouroux has rhizoids.
