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外植体取样时间对‘玉妃’和‘超红’桃初代培养的影响 被引量:1
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作者 王海荣 刘伟 +2 位作者 安淼 李桂祥 余贤美 《山东农业科学》 2014年第11期50-51,共2页
从2012年7月到2013年10月,以‘玉妃’和‘超红’桃当年生枝上带腋芽的茎段为外植体,研究了不同取样时间对初代培养的影响。结果表明,5月份取样‘玉妃’和‘超红’桃的污染率最低,分别为16%和10%;成活率最高,分别为84%和60%以上。5月份... 从2012年7月到2013年10月,以‘玉妃’和‘超红’桃当年生枝上带腋芽的茎段为外植体,研究了不同取样时间对初代培养的影响。结果表明,5月份取样‘玉妃’和‘超红’桃的污染率最低,分别为16%和10%;成活率最高,分别为84%和60%以上。5月份是最佳取样时间。 展开更多
关键词 '玉妃’桃 '超红’ 取样时间 初代培养
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温室栽培桃树新品种‘超红’
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作者 张安宁 王金政 《果农之友》 2004年第6期13-13,15,共2页
关键词 温室栽培 桃树 '超红’ 特征特性 产量表现 栽培技术
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‘超红’桃在金华地区引种表现及优质栽培技术
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作者 陈一帆 沈建生 林贤锐 《中国园艺文摘》 2010年第8期154-155,共2页
‘超红'桃在浙江省金华市的试种观察表明,该品种具有果面浓红,全果着色,外观独特,风味甘甜爽口,肉质硬脆,早熟、丰产、耐贮运和果实成熟后挂树时间久等特点,熟期为5月下旬~6月上旬,采摘期可长达20d左右,弥补了现有‘大观1号'... ‘超红'桃在浙江省金华市的试种观察表明,该品种具有果面浓红,全果着色,外观独特,风味甘甜爽口,肉质硬脆,早熟、丰产、耐贮运和果实成熟后挂树时间久等特点,熟期为5月下旬~6月上旬,采摘期可长达20d左右,弥补了现有‘大观1号'和‘金华大白桃'采收期短、果肉软化快、不耐贮运的缺点,综合性状表现良好。主要介绍其引种表现,并对其优质丰产栽培技术进行总结概括。 展开更多
关键词 '超红’ 栽培技术
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‘超早红’樱桃引种表现及栽培要点 被引量:1
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作者 徐建峰 《中国园艺文摘》 2010年第12期179-179,185,共2页
江苏太仓地区2006年引入‘超早红’樱桃试栽,其引种表现为:平均单果重4g,最大果重4.7g,含可溶性固形物19.6%;定植当年可形成花芽,翌年即可结果;花期3月上旬,果实4月中旬成熟,属于极早熟品种。
关键词 '红’樱桃 引种 栽培
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‘超早红’樱桃冬季温室育苗试验
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作者 任泽飞 许廷全 +3 位作者 李晓泉 孙爱华 贾丽 陈锦璞 《中国果菜》 2016年第9期66-69,共4页
本文介绍了我国樱桃新品种‘超早红’冬季温室育苗技术。10月下旬将当年生大青叶樱桃苗移栽到直径16cm的黑色塑料营养钵中并摆放在半地下式温室贮苗台上,11月下旬扣棚升温,12月中旬加盖防寒被保温。翌年1月上旬采集‘超早红’樱桃一年... 本文介绍了我国樱桃新品种‘超早红’冬季温室育苗技术。10月下旬将当年生大青叶樱桃苗移栽到直径16cm的黑色塑料营养钵中并摆放在半地下式温室贮苗台上,11月下旬扣棚升温,12月中旬加盖防寒被保温。翌年1月上旬采集‘超早红’樱桃一年生枝条,截取2个芽的枝段作为接穗,确保其中至少一个芽为叶芽,蜡封后采用切腹接的方法嫁接,嫁接成活率平均为97.14%,6月上旬平均苗高83.29cm,根颈部平均直径0.81cm,成品苗率92.38%。 展开更多
关键词 '红’樱桃 育苗 温室
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‘超红短枝’油桃
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作者 李培环 董晓颖 李书华 《果农之友》 2003年第9期43-43,共1页
关键词 '短枝’油桃 特征特性 产量表现 栽培技术
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油桃早熟新品种‘朝霞’的选育 被引量:2
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作者 高东升 肖伟 +4 位作者 武红玉 李玲 陈修德 李冬梅 刘立常 《中国果树》 2016年第2期60-62,I0002,共4页
‘朝霞’是油桃品种‘超红珠’早熟单株芽变。果实近圆形,平均单果重124.0 g,最大单果重195.2 g;果实浓红色,光滑无毛,果顶尖,缝合线明显;黏核,果肉白色,软溶质,风味甜,品质优良;可溶性固形物含量12.10%,维生素C含量69.80 mg/kg,耐贮运... ‘朝霞’是油桃品种‘超红珠’早熟单株芽变。果实近圆形,平均单果重124.0 g,最大单果重195.2 g;果实浓红色,光滑无毛,果顶尖,缝合线明显;黏核,果肉白色,软溶质,风味甜,品质优良;可溶性固形物含量12.10%,维生素C含量69.80 mg/kg,耐贮运;在山东省莒县,露地栽培果实5月下旬至6月上旬成熟,果实发育期49~52 d;适宜在山东省露地、设施促成栽培。2014年通过山东省林木品种审定委员会审定。 展开更多
关键词 油桃 品种 '朝霞’ 早熟 '珠’
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Physiology and Ultrastructure of Azolla imbricata as Affected by Hg 2+ and Cd 2+ Toxicity 被引量:27
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作者 施国新 徐勤松 +5 位作者 解凯彬 徐楠 张小兰 曾晓敏 周红卫 朱蕾 《Acta Botanica Sinica》 CSCD 2003年第4期437-444,共8页
The toxic effects of different gradient concentrations of Hg2+ and Cd2+ on chlorophyll content, chlorophyll a/b value, photosynthetic O-2 evolution, respiration rate, anti-oxidase system (superoxide dismulase (SOD), c... The toxic effects of different gradient concentrations of Hg2+ and Cd2+ on chlorophyll content, chlorophyll a/b value, photosynthetic O-2 evolution, respiration rate, anti-oxidase system (superoxide dismulase (SOD), catalase (CAT), peroxidase (POD)) and ultrastructure of the cells of Azolla imbricata (Roxb.) Nakai were studied. The results showed that with Hg2+ and Cd2+ increase, chlorophyll content and chlorophyll a/b value, photosynthetic O-2 evolution decreased drastically; respiration rate peaked at 2 mg/L pollutant and declined afterwards. The activities of SOD, CAT and POD increased first and decreased afterwards except the activity of POD, which decreased with the increasing of Cd2+ concentration. Ultrastructural observation showed that the extent of ultrastructural damage was much more serious with higher pollutant concentration and longer time of stress. This resulted in swelling of chloroplast, disruption and disappearance of chloroplast membrane and disintegration of chloroplasts; swelling of cristae of mitochondria, deformation and vacuolization of mitochondria; condensation of chromatin in nucleus, dispersion of nucleolus and disruption of nuclear membrane. The experimental results showed: (1) Hg2+ and Cd2+ pollution not only destroyed physiological activities, but also caused irreversible damage to its ultrastructure, thus leading the cells to death; (2) With increase in the stress of Hg2+ and Cd2+, ultrastructural damage was related to the changes of plant physiology; (3) The toxic symptoms of plant showed an evident correlation between dose and effect; (4) The toxicity of Cd2+ on A. imbricata is heavier than that of Hg2+ under the same treatment time and concentration. The lethal concentration of Hg2+ to A. imbricata ranged from 3.5 to 4 mg/L, and that of Cd2+ ranged from 3 to 3.5 mg/L. The damage of cell ultrastructure on Anabaena azollae Strasburger was observed. The results indicated that tolerance of Azolla imbricata for Hg2+ and Cd2+ was higher than that of A. imbricata. 展开更多
关键词 Azolla imbricata Hg2+ Cd2+ PHYSIOLOGY ULTRASTRUCTURE
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Ultrastructure Features of Sperm Cell in Amaryllis vittata
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作者 蔡雪 魏令波 《Developmental and Reproductive Biology》 1996年第1期49-54,T001-T002,共8页
Ultrastructure of sperm cells in pollen tube of Amaryllis vittata Ait. has been investigated in details by electron microscopy ,with particular emphasis on the organization and distribution of microtubules.The two new... Ultrastructure of sperm cells in pollen tube of Amaryllis vittata Ait. has been investigated in details by electron microscopy ,with particular emphasis on the organization and distribution of microtubules.The two newly formed sperm cells are arranged in tandem and sometimes in transverse at the right angle to the long axis of the pollen tube.Thevegetative nucleus is ahead and closely associated with the two sperm cells in all examined pollen tubes. The microtubules are distributed in the region between the common cell wall and the proximity of the sperm nucleus,they are singles and dispersed with mainly orientation of longitudinal and oblique,forming a loose bucket-like structure as a whole.In late stage of development,all of the microtubule array longitudinally and enclose the sperm nucleus.This configuration is very similar to the basket-like structure of microtubule in the generative cell. These results show that the organization and distribution of microtubules in the sperm cells are dynamic during cell development. 展开更多
关键词 Amaryllis vittata Sperm cell MICROTUBULE Ultrastructure.
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Ultrasonic-associated extraction of water soluble polysaccharides from defatted Korean pine kernel 被引量:4
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作者 陈小强 张莹 《Journal of Forestry Research》 SCIE CAS CSCD 2007年第2期133-135,共3页
Experiments of ultrasonic-associated extraction and hot water extraction of water soluble polysaccharides from defatted Korean pine (Pinus koraiensis) were conducted in Northeast Forestry University, Harbin, China. ... Experiments of ultrasonic-associated extraction and hot water extraction of water soluble polysaccharides from defatted Korean pine (Pinus koraiensis) were conducted in Northeast Forestry University, Harbin, China. The factors affecting extraction yield and content, such as extracting temperature, extracting time, the ratio of water to defatted kernel and concentration of ethanol were analyzed under specific condition. The optimal extracting parameters for ultrasound-associated extraction were determined as the ultrasonic temperature 70℃, the ratio of defatted kernel to water 1:20, the extracting time 40 min, and ethanol concentration 80%. Under such extraction conditions, the yield of water soluble polysaccharide was 3.65% and the average content of polysaccharide was 45.38% in the raw polysaccharides which gained in the experiment. Both extraction yield and content of polysaccharides extracted with ultrasound-associated extraction were higher than that with hot water extraction. The study demonstrates that ultrasound is a reliable and extremely effective tool for the fast extraction of water soluble polysaccharide of Korean pine kernel. 展开更多
关键词 ULTRASONIC Defatted Korean pine kernel POLYSACCHARIDES
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Direct Vibrational Energy Transfer in Monomeric Water Probed with Ultrafast Two Dimensional Infrared Spectroscopy
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作者 周德霞 魏千顺 +1 位作者 边红涛 郑俊荣 《Chinese Journal of Chemical Physics》 SCIE CAS CSCD 2017年第6期619-625,I0001,共8页
Vibrational relaxation dynamics of monomeric water molecule dissolved in d-chloroform solution were revisited using the two dimensional Infrared (2D IR) spectroscopy. The vibrational lifetime of OH bending in monome... Vibrational relaxation dynamics of monomeric water molecule dissolved in d-chloroform solution were revisited using the two dimensional Infrared (2D IR) spectroscopy. The vibrational lifetime of OH bending in monomeric water shows a bi-exponential decay. The fast compo- nent (T1=(1.2±0.1) ps) is caused by the rapid population equilibration between the vibrational modes of the monomeric water molecule. The slow component (T2=(26.4±0.2) ps) is mainly caused by the vibrational population decay of OH bending mode. The reorientation of the OH bending in monomeric water is determined with a time constant of t=(1.2±0.1) ps which is much faster than the rotational dynamics of water molecules in the bulk solution. Furthermore, we are able to reveal the direct vibrational energy transfer from OH stretching to OH bending in monomeric water dissolved in d-chloroform for the first time. The vibrational coupling and relative orientation of transition dipole moment between OH bending and stretching that effect their intra-molecular vibrational energy transfer rates are discussed in detail. 展开更多
关键词 Vibrational energy transfer Confined water Ultrafast Infrared spectroscopy Monomeric water
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Ultrafast Infrared Spectroscopic Study of Microscopic Structural Dynamics in pH Stimulus-Responsive Hydrogels 被引量:1
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作者 Jian Hong De-xia Zhou +2 位作者 Hong-xing Hao Min Zhao Hong-tao Bian 《Chinese Journal of Chemical Physics》 SCIE CAS CSCD 2020年第5期540-546,I0078,共8页
Hydrogels show versatile properties and are of great interest in the fields of bioelectronics and tissue engineering.Understanding the dynamics of the water molecules trapped in the three-dimensional polymeric network... Hydrogels show versatile properties and are of great interest in the fields of bioelectronics and tissue engineering.Understanding the dynamics of the water molecules trapped in the three-dimensional polymeric networks of the hydrogels is crucial to elucidate their mechanical and swelling properties at the molecular level.In this report,the poly(DMAEMA-co-AA)hydrogels were synthesized and characterized by the macroscopic swelling measurements under different pH conditions.Furthermore,the microscopic structural dynamics of pH stimulus-responsive hydrogels were studied using FTIR and ultrafast IR spectroscopies from the viewpoint of the SCN-anionic solute as the local vibrational reporter.Ultrafast IR spectroscopic measurements showed the time constants of the vibrational population decay of SCN-were increased from 14±1 ps to 20±1 ps when the pH of the hydrogels varied from2.0 to 12.0.Rotational anisotropy measurements further revealed that the rotation of SCNanionic probe was restricted by the three-dimensional network formed in the hydrogels and the rotation of SCN-anionic probe cannot decay to zero especially at the pH of 7.0.These results are expected to provide a molecular-level understanding of the microscopic structure of the cross-linked polymeric network in the pH stimulus-responsive hydrogels. 展开更多
关键词 Ultrafast IR spectroscopy HYDROGEL pH stimulus responsive Structural dynamics
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Ultrasound-associated extraction of seed oil of Korean pine 被引量:37
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作者 ZHANGYing WANGZhen-yu CHENXiao-qiang 《Journal of Forestry Research》 SCIE CAS CSCD 2005年第2期140-142,共3页
Experiment on ultrasound- associated extraction of seed oil of Korean pine(Pinus koraiensis) was conducted in Northeast Forestry University, Harbin, China. The factorsaffecting extraction yield, such as ultrasonic fre... Experiment on ultrasound- associated extraction of seed oil of Korean pine(Pinus koraiensis) was conducted in Northeast Forestry University, Harbin, China. The factorsaffecting extraction yield, such as ultrasonic frequency, extracting temperature, extracting timeand the ratio of material to liquid (ratio of Korean pine seed to absolute alcohol), were analyzedunder specific condition and the optimal extracting parameters were obtained as the ultrasonicfrequency 32 000 Hz, the extracting temperature 80 ℃, the extracting time 50 min, and the ratio ofmaterial to liquid 1: 30. The study demonstrates that ultrasound is a reliable and great efficiencytool for the fast extraction of Korean pine seed oil. 展开更多
关键词 ULTRASONIC EXTRACTION korean pine seed oil influencing factors
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Polarization Dependent Time-Resolved Infrared Spectroscopy and Its Applications
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作者 张文凯 《Chinese Journal of Chemical Physics》 SCIE CAS CSCD 2016年第1期1-9,I0001,共10页
Polarization dependent time-resolved infrared (TRIR) spectroscopy has proven to be a useful technique to study the structural dynamics in a photochemical process. The angular information of transient species is obta... Polarization dependent time-resolved infrared (TRIR) spectroscopy has proven to be a useful technique to study the structural dynamics in a photochemical process. The angular information of transient species is obtainable in this measurement, which makes it a valuable technique for the investigation of electron distribution, molecular structure, and conformational dynamics. In this review, we briefly introduce the principles and applications of polarization dependent TRIR spectroscopy. We mainly focused on the following topics: (i) an overview of TRIR spectroscopy, (ii) principles of TRIR spectroscopy and its advantages compared to the other ultrafast techniques, (iii) examples that use polarization dependent TRIR spectroscopy to probe a variety of cheinical and dynamical phenomena including protein conformational dynamics, excited state electron localization, and photoisomerization, (iv) the limitations and prospects of TRIR spectroscopy. 展开更多
关键词 Ultrafast spectroscopy Infrared spectroscopy POLARIZATION Time-resolved infrared spectroscopy
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Infrared Absorption and [CuO_5] Pyramid of High T_c Superconductor Bi-Sb-Sr-Ca-Cu-O System
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作者 宋桂兰 朱正芳 白培光 《Journal of Beijing Institute of Technology》 EI CAS 1996年第1期63-67,共5页
The infrared absorption spectra of different superconducting phase of high Tc super conductor Bi-Sb-Sr-Ca-Cu-O have been measured . The results show that only in the range of 400cm-1 -700cm-1,there is a group of peaks... The infrared absorption spectra of different superconducting phase of high Tc super conductor Bi-Sb-Sr-Ca-Cu-O have been measured . The results show that only in the range of 400cm-1 -700cm-1,there is a group of peaks which changes with different superconducting phases.According to group theory and infrared spectra of CuO, this group of peaks could be assigned to be the [CuO6]octahedron, the [CuO5] pyramid and the [CuO4]plane quadrilateral,but not CU-O plane or CU-O chain. Furthermore, the quasi-three dimensional Cu-O layers consisting of [CuO5] pyramids and proper coupling between them are essential factor for high Tc. It seems that the weaker compling of layers, the higher Tc 展开更多
关键词 infrared absorption high temperature superconductivity [CuO_5] pyramid structure
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Ultrastructure of Kupffer cells and hepatocytes in the Dubin-Johnson syndrome:A case report 被引量:1
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作者 Maria Elzbieta Sobaniec-Lotowska Dariusz Marek Lebensztein 《World Journal of Gastroenterology》 SCIE CAS CSCD 2006年第6期987-989,共3页
Ultrastructure of Kupffer cells and hepatocytes in liver bioptate was evaluated in a 17-year-old boy with Dubin -Johnson syndrome (D.1S). The liver tissue obtained by needle biopsy was fixed in glutaraldehyde and pa... Ultrastructure of Kupffer cells and hepatocytes in liver bioptate was evaluated in a 17-year-old boy with Dubin -Johnson syndrome (D.1S). The liver tissue obtained by needle biopsy was fixed in glutaraldehyde and paraformaldehyde and routinely processed for electron microscopic analysis. The ultrastructural examinations of liver bioptate revealed the accumulation of membranebound, electron-dense lysosomal granules within the cytoplasm of hepatocytes, characteristic of D.1S. They were located mainly in the vicinity of the biliary pole, and preferentially in the centrilobular region that corresponded to the pigment deposits seen under light microscope. The presence of the granules was accompanied by dilated elements of the granular endoplasmic reticulum and paracrystalline mitochondrial inclusions as well as dilation of the bile canaliculi. The changes in hepatocytes coexisted with marked stimulation and enhanced phagocytic activity of Kupffer cells. This was manifested in the accumulation of pigment deposits within their cytoplasm that corresponded to those observed in hepatocytes. Hyperactive pericentral Kupffer cells which are involved in the response to pigmentary material originating from disintegrated hepatocytes may play an essential role in the development of DJS. 展开更多
关键词 Liver biopsy Electron microscopic study Lysosomal granules Functional hyperbilirubinemia Dubin-Johnson syndrome
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Effects of Different Storage Methods on the Fruit Quality and Physiological Changes in Yihong Honey Peach 被引量:1
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作者 CHEN Wei-feng YANG Yu +1 位作者 LONG Li-ping DENG Wen 《Agricultural Science & Technology》 CAS 2022年第4期15-24,共10页
Taking Yihong,the main cultivar of mid-ripening honey peaches(Prunus persica)in Hunan,as material,this study explored the effects of different storage methods on the fruit quality and physiological changes in Yihong h... Taking Yihong,the main cultivar of mid-ripening honey peaches(Prunus persica)in Hunan,as material,this study explored the effects of different storage methods on the fruit quality and physiological changes in Yihong honey peach by the combination of polyethylene film packaging with low-temperature storage.The results showed that the weight loss rate of packaged storage at normal temperature(T1)was significantly lower than that of unpackaged storage at normal temperature(CK),but there was no significant difference in other indicators.In the middle of storage,the respiration rate and ethylene release of packaged storage at 0℃(T3)were extremely significantly lower or significantly lower than those of unpackaged storage at 0℃(T2),packaged storage at 0℃after cold adapting at 8℃for 5 d(T4),and packaged storage in a fluctuating temperature range of 0~3.5℃(T5).After storage,the structure of the middle lamella in the cell wall of T3 was compact and uniform,and the mitochondrial structure was complete,while the cell walls and mitochondria of T4 and T5 were severely deformed and disintegrated.The activities of antioxidant enzymes peroxidase(POD)and superoxide dismutase(SOD)of T3 was significantly higher than those of T4 and T5,and the content of malondialdehyde(MDA)and polyphenol oxidase(PPO)of T3 was also the lowest.Therefore,0°C packaged storage can effectively delay the senescence of Yihong honey peach and prolong its freshness period. 展开更多
关键词 Honey peach(Prunus persica L.Batsch) Yihong Packaging Storage methods Physiological index ULTRASTRUCTURE
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Is iron overload in alcohol-related cirrhosis mediated by hepcidin? 被引量:2
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作者 Tariq Iqbal Azzam Diab +4 位作者 Douglas G Ward Matthew J Brookes Chris Tselepis Jim Murray Elwyn Elias 《World Journal of Gastroenterology》 SCIE CAS CSCD 2009年第46期5864-5866,共3页
In this case report we describe the relationship between ferritin levels and hepcidin in a patient with alcohol-related spur cell anemia who underwent liver transplantation.We demonstrate a reciprocal relation-ship be... In this case report we describe the relationship between ferritin levels and hepcidin in a patient with alcohol-related spur cell anemia who underwent liver transplantation.We demonstrate a reciprocal relation-ship between serum or urinary hepcidin and serum ferritin,which indicates that inadequate hepcidin production by the diseased liver is associated with elevated serum ferritin.The ferritin level falls with increasing hepcidin production after transplantation.Neither inflammatory indices(IL6)nor erythropoietin appear to be related to hepcidin expression in this case.We suggest that inappropriately low hepcidin production by the cirrhotic liver may contribute substantially to elevated tissue iron stores in cirrhosis and speculate that hepcidin replacement in these patients may be of therapeutic benefi t in the future. 展开更多
关键词 ALCOHOL IRON ANAEMIA HEPCIDIN CIRRHOSIS
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Measurement and Correlation of Solubilities of C.I. Disperse Red 73,C.I. Disperse Blue 183 and Their Mixture in Supercritical Carbon Dioxide 被引量:7
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作者 郑金花 徐明仙 +1 位作者 鲁雪燕 林春绵 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2010年第4期648-653,共6页
The solubility of disperse dyes and their mixture in supercritical carbon dioxide is an important property in study and development of supercritical fluid dyeing technology.In this study,solubilities of C.I.Disperse R... The solubility of disperse dyes and their mixture in supercritical carbon dioxide is an important property in study and development of supercritical fluid dyeing technology.In this study,solubilities of C.I.Disperse Red 73,C.I.Disperse Blue 183 and their mixture in supercritical CO2 are measured at temperatures from 343.2 to 383.2 K and pressures from 12 to 28 MPa with a static recirculation method.Under the experimental conditions for the binary(C.I.Disperse Red 73+CO2 or C.I.Disperse Blue 183+CO2) and ternary(C.I.Disperse Red 73+C.I.Disperse Blue 183+CO2) systems,the solubilities increase with pressure.The solubility of C.I.Disperse Blue 183 decreases with the increase of temperature when the pressure is lower than 16 MPa,and the trend is opposite when the pressure is higher than 16 MPa.However,there is no crossover pressure for C.I.Disperse Red 73.The solubilities are also affected by molecular polarity of dyes.The co-solvent effect exhibited in the dissolving process of mixed dyes promotes their dissolution in supercritical CO2.The experimental data of solubilities of C.I.Disperse Red 73,C.I.Disperse Blue 183,and their mixture are correlated with the Chrastil model and Mendez-Santiago/Teja model. The former is more accurate. 展开更多
关键词 supercritical CO2 SOLUBILITY Disperse Red 73 Disperse Blue 183
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Therapeutic effects of flavonoids from Ceylon green tea on hypoxic human brain epithelial cells
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作者 黄山红 Ranil De Silva 《Journal of Chinese Pharmaceutical Sciences》 CAS 2008年第4期324-331,共8页
We have extracted and purified flavonoids as active ingredients from Ceylon green tea (Dilmah). In this project, an in vitro hypoxic model using human brain epithelial cells (HBEC) was studied with treatment of th... We have extracted and purified flavonoids as active ingredients from Ceylon green tea (Dilmah). In this project, an in vitro hypoxic model using human brain epithelial cells (HBEC) was studied with treatment of the tea extract before inducing hypoxia. We have tested the hypothesis that flavonoids extracted from Ceylon green tea act as potential therapeutic ingredient (s) to reduce oxidative stress in hypoxic cells through its antioxidant properties and its ability to reduce cerebral cellular death. The biochemical antioxidant tests show that the Ceylon green tea has 68% ± 2.8% inhibition property of scavenging of ABTS. The inhibition of pyrogallol red bleaching by HOCI from Ceylon tea was 79% ± 4.5%. After exposing to hypoxia, the cell viability was 29% ± 2.3% in the hypoxia control group but 41% ± 4.7% for flavonoids extract treated group. In LDH assay, flavonoids extract treated group had 75% ± 3.7% reducing of LDH release. The flavonoids extract treated groups significantly increased in antioxidant enzyme activity assays: the activity level of SOD [(1.5 ± 0.6) μmol/min/mg protein], CAT [(0.61 ± 0.06) μmol/min/mg protein], GPx [(2.6 ± 0.41) μmol/min/mg protein] and GST [(6.0 ± 2.4) μmol/min/mg protein] are significantly increased as compared with hypoxic control [(0.5 ± 0.52, 0.51 ± 0.04, 1.2 ± 0.35 and 3.1 ± 1.6) μmol/min/mg protein, respectively]. The study demonstrated a great clinical potential and opened a new avenue to prevent stroke by drinking Ceylon tea. 展开更多
关键词 Stroke HYPOXIA Flavonoids extract ABTS Pyrogallol red Lactate dehydrogenase Superoxide dismutase CATALASE
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