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‘金玉’猕猴桃组织培养研究
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作者 吕海燕 李大卫 +1 位作者 李黎 钟彩虹 《湖北民族学院学报(自然科学版)》 CAS 2019年第4期361-364,共4页
为建立‘金玉’猕猴桃离体快繁体系,以‘金玉’猕猴桃嫩枝顶芽、带腋芽茎段、叶片、叶柄为材料进行离体培养诱导,获得无菌苗.以获得的无菌苗叶片为外植体,探讨了不同植物生长调节剂种类及质量浓度组合对不定芽诱导形成的影响,并对组培... 为建立‘金玉’猕猴桃离体快繁体系,以‘金玉’猕猴桃嫩枝顶芽、带腋芽茎段、叶片、叶柄为材料进行离体培养诱导,获得无菌苗.以获得的无菌苗叶片为外植体,探讨了不同植物生长调节剂种类及质量浓度组合对不定芽诱导形成的影响,并对组培苗不定根诱导作出初步探究.结果表明,资源离体保存最佳外植体为茎顶端.无菌苗叶片不定芽再生最佳培养基为MS+TDZ(2.0 mg/L)+IAA(0.5 mg/L),不定芽平均再生率为74.68%.不定芽经过伸长生长,取2~3 cm高幼苗进行生根诱导,不定根再生率达85.1%,平均根数为4~5条.本研究成功对‘金玉’进行了离体保存,并初步建立其再生繁育体系,为今后新优猕猴桃品种培育奠定基础. 展开更多
关键词 '金玉’ 猕猴桃 叶片再生 生根培养
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猕猴桃新品种‘金玉’有机种植技术 被引量:2
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作者 程杰元 李思胖 +4 位作者 吴小琴 黄大明 吴细卯 张熔 何中华 《中国园艺文摘》 2017年第5期199-201,共3页
文章主要介绍猕猴桃新品种‘金玉’有机种植技术,包括增施有机肥、果实套袋、物理治虫、绿肥种植、人工除草等技术,以达到提高果实品质的应用效果。
关键词 猕猴桃 '金玉’ 有机认证 生产技术
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氮、磷、钾肥配施对桂花品种‘金玉台阁’开花性状及叶片中叶绿素和营养元素含量的影响 被引量:8
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作者 齐豫川 潘远智 +3 位作者 杨亚男 鲜小林 陈睿 刘柿良 《植物资源与环境学报》 CAS CSCD 北大核心 2017年第2期35-45,共11页
以桂花品种‘金玉台阁’(Osmanthus fragrans‘Jinyu Taige’)盆栽苗为供试材料,采用"3414"肥料效应试验设计方案,研究了不同单株施用量CO(NH_2)_2(0.0、1.0、2.0和3.0 g)、NH_4H_2PO_4(0.0、1.5、3.0和4.5 g)和KCl(0.0、0.5... 以桂花品种‘金玉台阁’(Osmanthus fragrans‘Jinyu Taige’)盆栽苗为供试材料,采用"3414"肥料效应试验设计方案,研究了不同单株施用量CO(NH_2)_2(0.0、1.0、2.0和3.0 g)、NH_4H_2PO_4(0.0、1.5、3.0和4.5 g)和KCl(0.0、0.5、1.0、1.5 g)对秋冬生长期(2014年10月至2015年2月)‘金玉台阁’开花性状及叶片中叶绿素和营养元素的影响进行了比较和分析,并筛选出适宜的氮、磷、钾肥施用量。结果表明:与CK[CO(NH_2)_2、NH_4H_2PO_4和KCl的单株施用量均为0.0 g]组相比,各氮、磷、钾肥配施处理组‘金玉台阁’的单株产花量、花瓣相对含水量、花径以及叶片中叶绿素、全氮、全磷和全钾的含量总体上显著升高。CO(NH_2)_2单株施用量对‘金玉台阁’单株产花量、叶片中叶绿素含量和全氮含量的影响最大,NH_4H_2PO_4单株施用量对这3个指标的影响次之;NH_4H_2PO_4单株施用量对‘金玉台阁’叶片中全磷含量的影响最大;KCl单株施用量对‘金玉台阁’花瓣相对含水量、花径及叶片中全钾含量的影响最大。在秋冬生长期内叶片中叶绿素含量呈先降低后升高的趋势,均在12月份降至最低值。通过建立‘金玉台阁’单株产花量(y)与CO(NH_2)2、NH_4H_2PO_4和KCl的单株施用量(分别为x_N、x_P和x_K)间的肥料效应模型,得到的三元二次肥料效应模型为y=1.202 7+0.756 1×x_N+0.263 2×x_P+0.459 0×x_K-0.276 1×x_N^2-0.120 1×x_P^2-0.500 7×x_K^2+0.190 1×x_N×xP-0.096 0×x_N×x_K+0.118 5×x_P×x_K。以单株产花量为目标,‘金玉台阁’的CO(NH_2)2、NH_4H_2PO_4和KCl的最佳单株施用量分别为2.58、3.56和0.86 g。 展开更多
关键词 桂花品种'金玉台阁’ 氮、磷、钾肥配施 “3414”肥料效应试验设计方案 单株产花量 营养元素 肥料效应模型
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切花菊新品种金玉蝶选育与栽培技术
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作者 孙文松 张晓波 《辽宁农业科学》 2014年第2期86-87,共2页
金玉蝶是以辽菊1号为母本、比华利14为父本,经人工杂交得到的黄色托桂型多头切花菊新品种。该品种生长势强,出花率高,抗锈病、蚜虫。适宜在辽宁地区保护地栽培。
关键词 切花菊 '金玉蝶’ 选育
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Study on Species and Behaviors of Flowervisiting Insects on Mussaenda pubescens
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作者 何应森 徐晓燕 李昕然 《Agricultural Science & Technology》 CAS 2016年第5期1200-1203,共4页
[Objective] This study aimed to understand the species and behaviors of flower-visiting insects on Mussaenda pubescens Ait.f.in the Mount Emei National Nature Reserve.[Method] The species,visiting frequency,visiting t... [Objective] This study aimed to understand the species and behaviors of flower-visiting insects on Mussaenda pubescens Ait.f.in the Mount Emei National Nature Reserve.[Method] The species,visiting frequency,visiting time and behaviors of flower-visiting insects on M.pubescens were studied by collection,identification and image capture.[Result] The flower-visiting insects on M.pubescens in the Mount Emei covered total 26 species with 21 species from Lepidoptera,2 species from Hymenoptera,and 3 species from Diptera.Thus,Lepidoptera were the main group of pollinators for c in the Mount Emei National Nature Reserve.The daily activity rhythms of Lepidoptera,Hymenoptera and Diptera showed different characteristics,and they were characterized by single-peak type and double-peak type.[Conclusion] This study will provide certain theoretical basis for studies on the diversity of flower-visiting insects on plants in the Mount Emei National Nature Reserve. 展开更多
关键词 M.pubescens Flower-visiting insects Flowering-visiting behavior Mount Emei
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“鼎铛玉石,金块珠砾”句法结构辨正
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作者 常志伟 《牡丹江师范学院学报(社会科学版)》 2013年第2期92-93,共2页
本文对"鼎铛玉石,金块珠砾"的句法结构作了较为合理的解释,认为"鼎铛玉石,金块珠砾"的句法结构是由四个表示比喻的判断句活用结构紧缩而成,属于判断句活用。
关键词 鼎铛玉石 金块珠砾 句法结构
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Process of aluminum dross recycling and life cycle assessment for Al-Si alloys and brown fused alumina 被引量:20
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作者 洪建平 王俊 +3 位作者 陈海 孙宝德 李家镜 陈冲 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2010年第11期2155-2161,共7页
In 2008,around 596 000 t of aluminum dross was generated from secondary aluminum industry in China;however,it was not sufficiently recycled yet.Approximately 95% of the Al dross was land filled without innocent treatm... In 2008,around 596 000 t of aluminum dross was generated from secondary aluminum industry in China;however,it was not sufficiently recycled yet.Approximately 95% of the Al dross was land filled without innocent treatment.The purpose of this work is to investigate Al dross recycling by environmentally efficient and friendly methods.Two methods of Al dross recycling which could utilize Al dross efficiently were presented.High-quality aluminum-silicon alloys and brown fused alumina(BFA) were produced successfully by recycling Al dross.Then,life cycle assessment(LCA) was performed to evaluate environmental impact of two methods of Al dross recycling process.The results show that the two methods are reasonable and the average recovery rate of Al dross is up to 98%.As the LCA results indicate,they have some advantages such as less natural resource consumption and pollutant emissions,which efficiently relieves the burden on the environment in electrolytic aluminum and secondary aluminum industry. 展开更多
关键词 aluminum dross life cycle assessment A1-Si alloys brown fused alumina
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Effects of Different Nitrogen Sources and Metal Ions on Biogas Generation by Corn Stalk Fermentation
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作者 齐辉阳 黄世臣 +1 位作者 王慧 李文浩 《Agricultural Science & Technology》 CAS 2016年第11期2525-2528,共4页
[Objective] The present study was conducted to explore the effects of dif- ferent nitrogen sources and metal ions on the biogas production process and biogas yield by fermentation of corn stalk powder using self-desig... [Objective] The present study was conducted to explore the effects of dif- ferent nitrogen sources and metal ions on the biogas production process and biogas yield by fermentation of corn stalk powder using self-designed anaerobic fermenta- tion system. [Method] The biogas produced during fermentation was collected by water displacement, and measured by ignition. [Result] Ammonium bicarbonate, urea and yeast extract powder all increased biogas yield. Among them, ammonium bi- carbonate was the best nitrogen source. Ammonium dihydrogen phosphate showed an inhibitory effect on biogas production. Biogas yield was significantly increased by adding NaCl, KCl, MgCl2 or CaCl2, among which, NaCl had the best effect. [Conclu- sion] It is not conducive to the growth and development of methanogenic flora in the production of biogas using corn stalks as the only material in fermentation. The biogas yield can be increased by adding exogenous nitrogen, which reduces the carbon to nitrogen (C:N) ratio of fermentation materials. The biogas yield can be significantly increased by adding appropriate types and amount of electrolytes, with appropriate C:N ratio. The methanogenic flora is more tolerant to NaCl and KCl than to MgCl2 and CaCl2. Moreover, NaCl can not only significantly increase the biogas production, but also shorten startup period of methanogenic flora, thereby shortening the gas producing cycle. 展开更多
关键词 FERMENTATION Corn stalk BIOGAS Nitrogen source Metal ion
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LITERATURE RESEARCH ON SCREENING OF THE NUCLEUS ACUPOINTS FOR TREATMENT OF INTELLECTUAL DISTURBANCES
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作者 陈永灿 杨楣良 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 1999年第2期83-88,共6页
Intelligence-benefiting acupuncturerefers to the acupuncture-moxibustiontreatment of intellectual disturbances byremoving obstructions in channels andcollaterals,regulating yin and yang,andeliminating pathogenic facto... Intelligence-benefiting acupuncturerefers to the acupuncture-moxibustiontreatment of intellectual disturbances byremoving obstructions in channels andcollaterals,regulating yin and yang,andeliminating pathogenic factors to strengthenthe body resistance,with the effects ofstrengthening the brain,benefiting intelligence, 展开更多
关键词 Acupuncture Points Acupuncture Therapy China DEMENTIA History 15th Century History 16th Century History Medieval Humans Intelligence Mental Retardation
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Effects of Heavy Metals on Ammonification, Nitrification and Denitrification in Maize Rhizosphere 被引量:5
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作者 CHENYINGXU YANGYE 《Pedosphere》 SCIE CAS CSCD 2001年第2期115-122,共8页
The ammonification, nitrification and denitrification in maize rhizosphere of alluvial soils were compared with those in the bulk soil after exposure to different kinds of heavy metals. The addition of cadmium at low ... The ammonification, nitrification and denitrification in maize rhizosphere of alluvial soils were compared with those in the bulk soil after exposure to different kinds of heavy metals. The addition of cadmium at low levels (2 mg kg-1 and 5 mg kg-1) could stimulate the ammonification and nitrification in the soils, while inhibition influences were found at high levels of Cd addition (10 mg kg-1 and 20 mg kg-1). The relationship between microbial activity and cadmium concentration varied with the kind of microorganisms. The nitrifying bacteria were more sensitive to cadmium pollution than the ammonifying bacteria. When Cd(II), Cu(II) and Cr(VI) were compared at the same addition concentration of 20 mg kg-1 soil, Cd(II) was the most effective inhibitor of ammonification and denitrification among the three investigated heavy metals, and Cr(VI) had the most strong inhibitory influence on the nitrifying bacteria. The microbial activities in rhizosphere were higher than those in the bulk soil for most of the treatments. Cr(VI) proved to be the most effective in enhancing the microbial activities in rhizosphere, and this could be caused by the positive reduction of Cr(VI) to Cr(III) in rhizosphere, and the relatively sufficient existence of organic matter which intensified the adsorption of the metal. It seemed that the rhizosphere had some mitigation effect on heavy metal toxicity. 展开更多
关键词 AMMONIFICATION DENITRIFICATION heavy metal pollution NITRIFICATION RHIZOSPHERE
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Knowledge, Attitude and Practices Used in the Control of Striga in Maize by Smallholder Farmers of Western Kenya
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作者 Godfrey Nakitare Nambafu Richard Ndemo Onwonga +3 位作者 George Njomo Karuku Emmanuel Safaris Ariga Bernard Vanlauwe Kristina Roing de Nowina 《Journal of Agricultural Science and Technology(B)》 2014年第3期237-248,共12页
The production of maize is constrained by parasitic weeds, particularly Striga. A study was carried out to document farmers' knowledge, attitude and practices on Striga control among smallholder farmers across three ... The production of maize is constrained by parasitic weeds, particularly Striga. A study was carried out to document farmers' knowledge, attitude and practices on Striga control among smallholder farmers across three districts: Kisumu West, Busia and Teso South of Western Kenya. A multistage sampling technique was used to select the locations and farmers to be interviewed. A semi structured, open and closed ended questionnaire was administered leading to field experiment. Besides village meetings (39.2%), farmers got informed on farming methods under Striga weed farms and its control technologies through neighbours (2.5%), workshops and trainings (5.0%), field schools (3.7%), media (7.5%) and extension agents (10.8%). The attitudes of farmers towards Striga control varied but frequently cited: long term viability of the Striga seed (12.5%), difficult to control sharing of farm tools (10.8%), expensive technologies (13.3%), lack of adequate information (18.3%), labour intensive (15.0%), large farms for use of push and pull technology (1.7%) and time consuming (12.5%). Framers used various Striga control practices but traditional methods (25%) were among the most used (25%). Concerted effort involving researchers, extension agents and private sector are, therefore, required for wide scale dissemination and adoption of the existing modem control technologies. 展开更多
关键词 ATTITUDE KNOWLEDGE PRACTICE striga control technologies.
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The Impact of Beauveria bassiana, Bacillus thuringiensis (Bt) Spray, Trichogramma pretiosum and Spinosad on the Lepidoptera: Crambidae, European Corn Borer (Ostrinia nubilalis)
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作者 R. D. Tembo D. M. Pavuk 《Journal of Agricultural Science and Technology(A)》 2011年第5期678-692,共15页
The research cxamined the effects of microbial and novel insecticidal control strategies and also Irichogramma pretiosum on the I,epidoptera: Crambidae, the European corn borer (ECB), (Ostrinia nubilalis). The tr... The research cxamined the effects of microbial and novel insecticidal control strategies and also Irichogramma pretiosum on the I,epidoptera: Crambidae, the European corn borer (ECB), (Ostrinia nubilalis). The treatments included ~ino^d, from bacteria the entomopathogenic fungus Beauveria bassiana, the egg parasitoid Trichogramma pretiosum, and foliar applied Bt spray, and t^vo combinations Bearveria bassiana plus Bt spray and Trichogramma pretiosum and spinosad. The infestations by the European corn borer larvae on stalks of corn were very" severe in all the control treatments both 2006 and 2007. The microbial treatments caused various levels of European corn borer mortality. In all the parameters there was a significant difference between the control and other treatments with a P 〈 0.00 l. In this research, treatment with spinosad emerged as the most effective biological agent in the control of the European corn borer. The treatments had no significant effects on the relative abundance and composition of non-target arthropods (P 〉 0.05). This research is relevant in boosting underutilized control strategies and increasing stakeholder adoption of integrated pest management practices and thereby' reducing the use of conventional insecticides especially for orgamc thrmers. This is very important especially in certain countries where the commumities are skeptical about consuming transgenic crops. 展开更多
关键词 European corn borer (Ostrinia nubilalis) Trichogramma pretiosum spmosad Beauveria bassiana Bt sprays corn
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“鼎铛玉石,金块珠砾”释义质疑 被引量:1
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作者 胡安顺 《广西民族大学学报(哲学社会科学版)》 CSSCI 北大核心 2011年第4期2-5,共4页
研究认为目前"视鼎如铛"一类的释义有误,有关其语法关系的几种说法亦均不确。"鼎铛""玉石""金块""珠砾"应分别指宝鼎、美玉、金砖和珍珠,其中"鼎铛"属并列关系,"玉... 研究认为目前"视鼎如铛"一类的释义有误,有关其语法关系的几种说法亦均不确。"鼎铛""玉石""金块""珠砾"应分别指宝鼎、美玉、金砖和珍珠,其中"鼎铛"属并列关系,"玉石、金块、珠砾"各属偏正关系。 展开更多
关键词 《阿房宫赋》 鼎铛玉石 金块珠砾 释义 语法
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Molecular characterization and efficacy evaluation of a transgenic corn event for insect resistance and glyphosate tolerance 被引量:7
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作者 Miao-miao LIU Xiao-jing ZHANG +2 位作者 Yan GAO Zhi-cheng SHEN Chao-yang LIN 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2018年第8期610-619,共10页
A transgenic maize event ZD12-6 expressing a Bacillus thuringiensis (Bt) fusion protein CrylAb/Cry2Aj and a modified 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) protein G10 was characterized and evaluated.... A transgenic maize event ZD12-6 expressing a Bacillus thuringiensis (Bt) fusion protein CrylAb/Cry2Aj and a modified 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) protein G10 was characterized and evaluated. Southern blot analysis indicated that ZD12-6 is a single copy integration event. The insert site was determined to be at chromosome 1 by border sequence analysis. Expression analyses of Bt fusion protein CrylAb/Cry2Aj and the EPSPS protein G10 suggested that they are both expressed stably in different generations. Insect bioassays demonstrated that the transgenic plants are highly resistant to Asian corn borer (Ostnnia furnacalis), cotton boll worm (Helicoverpa armigera), and armyworm (Mythimna separata). This study suggested that ZD12-6 has the potential to be developed into a commercial transgenic line. 展开更多
关键词 Transgenic maize Bacillus thuringiensis (Bt) Insect resistance Glyphosate tolerance
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Dual flow immunochromatographic assay for rapid and simultaneous quantitative detection of ochratoxin A and zearalenone in corn, wheat, and feed samples 被引量:11
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作者 Xian ZHANG Ke HE +6 位作者 Yun FANG Tong CAO Narayan PAUDYAL Xiao-feng ZHANG Hou-hui SONG Xiao-liang LI Wei-huan FANG 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2018年第11期871-883,共13页
A one-step dual flow immunochromatographic assay (DICGA), based on a competitive format, was de- veloped for simultaneous quantification of ochratoxin A (OTA) and zearalenone (ZEN) in corn, wheat, and feed sam- ... A one-step dual flow immunochromatographic assay (DICGA), based on a competitive format, was de- veloped for simultaneous quantification of ochratoxin A (OTA) and zearalenone (ZEN) in corn, wheat, and feed sam- ples. The limit of detection for OTA was 0.32 ng/ml with a detection range of 0.53-12.16 ng/ml, while for ZEN it was 0.58 ng/ml with a detection range of 1.06-39.72 ng/ml. The recovery rates in corn, wheat, and feed samples ranged from 77.3% to 106.3% with the coefficient of variation lower than 15%. Naturally contaminated corn, wheat, and feed samples were analyzed using both DICGA and liquid chromatography-tandem mass spectrometry (LC-MS/MS) and the correlation between the two methods was evaluated using a regression analysis. The DICGA method shows great potential for simple, rapid, sensitive, and cost-effective quantitative detection of OTA and ZEN in food safety control. 展开更多
关键词 Immunochromatographic assay Gold nanoparticles Ochratoxin A ZEARALENONE Quantification
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