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EMS诱变谷子‘长农35号’M_1代成熟期株型突变体的鉴定与分析 被引量:17
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作者 王军 杨慧卿 +1 位作者 袁峰 郭二虎 《中国农学通报》 CSCD 北大核心 2011年第18期84-89,共6页
为建立谷子突变体库,以便在谷子分子生物学研究中应用,以生产上主要推广种植的谷子品种‘长农35号’干种子为实验材料,采用0.8%和1.0%甲磺酸乙酯(EMS)进行诱变处理,试验共收获株型相关M1代突变体材料282份,其中,0.8%EMS诱变‘长农35号... 为建立谷子突变体库,以便在谷子分子生物学研究中应用,以生产上主要推广种植的谷子品种‘长农35号’干种子为实验材料,采用0.8%和1.0%甲磺酸乙酯(EMS)进行诱变处理,试验共收获株型相关M1代突变体材料282份,其中,0.8%EMS诱变‘长农35号’获得株型突变体材料100个单株,可分为10个组;1.0%EMS诱变‘长农35号’获得株型突变体材料182个单株,可分为17个组。M1代成熟期株型相关性状突变体分析结果表明:1.0%EMS处理‘长农35号’所获突变体,在株高、穗下节粗、穗下第一节间粗和茎节数4个性状与对照有显著差异,而0.8%处理与对照差异不显著。因此,对于‘长农35号’来讲,采用1.0%EMS进行诱变处理更有利于多类型大量突变单株的获得。 展开更多
关键词 谷子 '长农35号’ 甲基磺酸乙酯(EMS) 突变体 株型
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Identification and Analysis of Mature Plant Type Mutants of M_1 Generation Foxtail Millet Changnong35 Treated with EMS Mutagenesis 被引量:2
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作者 王军 袁峰 +5 位作者 杨慧卿 李瑜辉 范慧萍 王丽霞 张艾英 郭二虎 《Agricultural Science & Technology》 CAS 2011年第11期1628-1632,共5页
[Objective] The aim was to identify the mutants of millet Changnong35 induced by different concentrations of EMS, so as to construct a millet mutant library. [Method] Foxtail millet cultivar Changnong35 which is widel... [Objective] The aim was to identify the mutants of millet Changnong35 induced by different concentrations of EMS, so as to construct a millet mutant library. [Method] Foxtail millet cultivar Changnong35 which is widely used in agricultural production, was treated with 0.8% and 1.0% EMS; and then seven traits of mutants were investigated analyzed, to classify the mutants into different groups. [Result] 282 mutants in the M1 generation related to plant type were obtained, of which, 100 mutant plants treated with 0.8% EMS can be divided into 10 groups; 182 mutant plants obtained by using 1.0% EMS can be divided into 17 groups. The analysis results of the mature plant type traits of the M1 Generation showed that, plant height, diameter of stem under spike, diameter of the first internode under spike and internode number of the mutants treated with 1.0% EMS were significantly different from those of control, while those of mutants treated with 0.8% EMS did not show significant difference from those of control. [Conclusion] The inducing with 1.0% EMS was more conducive to obtain a large number and different types of mutants from Changnong35. 展开更多
关键词 Foxtail millet Changnong35 Ethyl Methane Sulphonate (EMS) MUTANTS Plant type
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