超声波处理花粉介导‘黄花’梨遗传转化体系的建立

【目的】建立超声波处理‘黄花’梨花粉介导插入外源基因的技术体系。【方法】以‘黄花’梨花粉为试材,检测不同蔗糖溶液中梨花粉破损率;通过中心组合设计方法,对超声波处理功率、处理时间、间隙时间、处理次数4个因子进行优化;利用含荧光蛋白基因的表达载体pCAMBIA 1304检测转化效率。【结果】梨花粉的适宜等渗溶液为15g.L-1蔗糖溶液。超声波处理的梨花粉受体最佳参数为：超声波处理功率160~170 W,处理时间6~7 s,间隙时间7 s,处理次数7次。采用加入含GFP（green fluorescent protein）基因的pCAMBIA 1304表达载体质粒检测优化的超声波处理组合,梨花粉外源基因的插入效率为3%。【结论】研究确定了超声波处理‘黄花’梨花粉遗传转化体系。

砂梨果皮转录组SNP位点发掘及其功能注释分析

从褐色砂梨品种‘黄花’(Pyrus pyrifolia Nakai‘Huanghua’)色泽形成期的果皮转录组数据开发SNP分子标记。检测了75764条unigene(总长度55676271bp)序列信息后,在25589条unigene(33.77%)中共发现SNP位点162048个,SNP发生频率为1/344bp,其中转换(transition)102342个,颠换(transversion)59706个。在所有变异类型中,A/G和C/T发生频率最高,分别达31.66%和31.50%。为进一步对得到的SNP所在基因功能进行分析,将包含SNP位点的25589条unigene序列注释到GenBank Nr蛋白数据库、Nt核苷酸数据库和Swiss-prot数据库中,并对其进行GO分类、COG分类注释和代谢通路注释(KEGG pathway),结合已有研究,筛选到1178个可能与果皮色泽形成相关的SNP标记。

Discussion on Development of Hainan Huanghuali(Dalbergia odorifera T.Chen)

On the morphological characteristics, growth environment and quality, rarity, and wood characteristics and value of Huanghuali, this paper discusses the development ways of Hainan Huanghuali It is suggested that Halnan Huanghuali could be well grown by guiding farmers to make full use of wasteland, slopes, scattered land, roadside and other resources; the landscaping greenbelt of the courtyard area and the residential area could be planned with nature as a main consideration for planting Huanghuali; and landscaping-type Huanghuali planting could make full use of local environment and surrounding local resources, highlighting the personalized landscaping greenbelt, forming good transition between residential area and urban space and creating a natural kindly living environment for the residents, so as to play the best ecological, social and economic benefits.