Objective: To observe the effect of inhibition oftelomerase activity by selenium dioxide (SeO2) on lungcarcinoma cell line GLC-82. Methods: TRAP-PCR-ELISA was used to study the changes of telomerase activity inhuman ...Objective: To observe the effect of inhibition oftelomerase activity by selenium dioxide (SeO2) on lungcarcinoma cell line GLC-82. Methods: TRAP-PCR-ELISA was used to study the changes of telomerase activity inhuman pulmonary adenocarcinoma cell line GLC-82 treated by SeO2 at the different concentrations (3, 10, 30 mmol/L) and for different times (24, 48, and 72 h). Results: SeO2inhibited the telomerase activity of GLC-82 at the different concentrations after treatment of 24, 48 and 72 h.Conclusion: SeO2 inhibits from telomerase activity ofhuman lung carcinoma line GLC-82. The effect of inhibition is dose-dependant and time-dependant.展开更多
Objective: This study was designed to determinewhether regulatory regions p250 of c-fos gene wereresponsive to SeO2 and to seek the possible mechanisms of regulation. Methods: HeLa cells were transfected withplasmids...Objective: This study was designed to determinewhether regulatory regions p250 of c-fos gene wereresponsive to SeO2 and to seek the possible mechanisms of regulation. Methods: HeLa cells were transfected withplasmids p250-tk CAT containing upstream regulatingregions of c-fos gene. Cells were treated by SeO2 for 20 min. CAT expression in transfected cells was observed by thinlayered chromatography. Results: In transfected HeLa cells CAT expression showed obvious increase after exposure to SeO2, especially in 10 mmol/L and 30 mmol/L group (P<0.05). Conclusion: Through affecting regulatory regions p250 of c-fos gene, SeO2 exerted biological effect on tumor cells. SeO2possibly had anti-tumor effects.展开更多
基金This work was supported by a grant from 9th five-year plan youth project of PLA (No.98Q010).
文摘Objective: To observe the effect of inhibition oftelomerase activity by selenium dioxide (SeO2) on lungcarcinoma cell line GLC-82. Methods: TRAP-PCR-ELISA was used to study the changes of telomerase activity inhuman pulmonary adenocarcinoma cell line GLC-82 treated by SeO2 at the different concentrations (3, 10, 30 mmol/L) and for different times (24, 48, and 72 h). Results: SeO2inhibited the telomerase activity of GLC-82 at the different concentrations after treatment of 24, 48 and 72 h.Conclusion: SeO2 inhibits from telomerase activity ofhuman lung carcinoma line GLC-82. The effect of inhibition is dose-dependant and time-dependant.
文摘Objective: This study was designed to determinewhether regulatory regions p250 of c-fos gene wereresponsive to SeO2 and to seek the possible mechanisms of regulation. Methods: HeLa cells were transfected withplasmids p250-tk CAT containing upstream regulatingregions of c-fos gene. Cells were treated by SeO2 for 20 min. CAT expression in transfected cells was observed by thinlayered chromatography. Results: In transfected HeLa cells CAT expression showed obvious increase after exposure to SeO2, especially in 10 mmol/L and 30 mmol/L group (P<0.05). Conclusion: Through affecting regulatory regions p250 of c-fos gene, SeO2 exerted biological effect on tumor cells. SeO2possibly had anti-tumor effects.
