喘可治注射液对COPD大鼠IL-12/IL-12R/STAT4信号通路的影响

目的观察喘可治注射液(CKZ)对慢性阻塞性肺疾病(COPD)大鼠肺组织IL-12/IL-12R/STAT4信号通路的影响。方法 50只SD大鼠随机分为正常组,模型组,CKZ高、中、低剂量(CKZ-H、CHZ-M、CKZ-L)组。除正常组外,各组复制COPD大鼠模型,并给予相应药物治疗,观察CKZ对COPD大鼠血清和肺泡灌洗液(BALF)中γ-干扰素(IFN-γ)、白细胞介素12(IL-12)、白细胞介素12受体(IL-12R)以及肺组织信号转导子和转录激活子4(STAT4)m RNA表达的影响。结果喘可治注射液各剂量组肺组织病理形态均有不同程度的改善,且降低COPD大鼠血清和BALF中IFN-γ、IL-12、IL-12R(除CKZ-L组BALF中IL-12R外)水平(P<0.05,P<0.01),可抑制大鼠肺组织STAT4 m RNA的表达(P<0.01,P<0.05)。结论喘可治注射液能一定程度改善COPD大鼠肺组织病理损害,其作用机制可能与其抑制IFN-γ、IL-12、IL-12R表达,干扰IL-12/IL-12R/STAT4信号通路对靶基因的激活,下调STAT4 m RNA表达,进而调节Th1/Th2分化失衡有关。

rhIL-12联合HBsAg刺激健康人外周血单核细胞对IL-12R的影响

目的确认重组人白细胞介素12(rhIL-12)联合乙型肝炎表面抗原(HBsAg)可增强健康人外周血单核细胞(PBMC)产生特异性细胞免疫应答,并分析其细胞来源,及对IL-12R的影响,探讨hIL-12联合HBsAg作为治疗型乙型肝炎疫苗的应用前景。方法 8名健康人PBMC分别与rhIL-12(1 ng/mL)、rHBsAg(500 ng/mL)和rhIL-12(1ng/mL)+rHBsAg(500 ng/mL)体外孵育,采用流式细胞术检测CD4+IFN-γ(γ-干扰素)+T细胞、CD8+IFN-γ+T细胞及CD56+IFN-γ+NK细胞百分比,并测定表达IL-12Rβ1、IL-12Rβ2的细胞百分比。结果 rhIL-12与rHBsAg联合组CD8+IFN-γ+T细胞及CD56+IFN-γ+NK细胞百分比均较对照组明显升高,且有非常显著差异(P<0.01),联合组CD8+IL-12Rβ1和CD56+IL-12Rβ1与单用rhIL-12或rHBsAg组比较均显著升高。结论提示rhIL-12联合rHBsAg诱生的IFN-γ主要来自CD56+NK细胞、CD8+T细胞,其次是CD4+T细胞;rhIL-12和rHBsAg单独均能上调健康人PBMCs表面IL-12R的表达,rhIL-12与rHBsAg联合具有增强作用。

基于RoyalTek REB-12R的GPS信号显示器设计

基于 RoyalTek REB-12R 接收机的 GPS 信号显示器,利用 MCU 处理 REB-12R 输出信号并在 LCD 显示。开机时 MCU 串口初始化使默认值格式符合 GPS,从 TXA 输出默认信号格式和输出信号格式。MCU 接收 GPS 的ASSIC 字符进入中断程序,然后判断数据起始位再送显示缓存。

A Convenient Method for Synthesis of Novel Cyclic Ethers (1R,2R,3R,5S,7S,9R,12R)-3-(t-Butyldimethylsilyl)oxy-7-methoxymethyl-oxy-2,10-dimethyl-12-oxatricyclo [7.2.1.0^(5,12)] dodecane

Novel cyclic esters (1R, 2R, 3R, 5S, 7S, 9R, 12R)-3-(t-butyldimethylsilyl)oxy-7- methoxymethyloxy-2, 10-dimethyl-12-oxatricyclo [7.2.1.05,12] dodecane were prepared when their precursor 1 was treated with SOCl2/pyridine. A plausible mechanism was hypothesized.

西洋参茎叶总皂苷氧化裂解产物中的新侧链环合型达玛烷型三萜

为了研究西洋参茎叶总皂苷氧化碱解产物的组成,对加拿大产西洋参茎叶总皂苷进行氧化碱解后,其产物通过硅胶柱色谱、Sephadex LH-20柱色谱、重结晶等方法进行分离纯化得到2个化合物。根据化合物的理化性质和光谱数据,鉴定其结构为:(12R,20S,24R)-20,24;12,24-diepoxy-24-deisopropyl-dammarane-3β-ol(1)和(20S,24R)-20,24-epoxydammarane-3β,12β,25-triol(2)。化合物1,2均为首次从西洋参茎叶总皂苷碱解产物中分离得到侧链环合的达玛烷型三萜,其中化合物1为新化合物。

SDN技术现状和未来前景分析

通过介绍SDN技术的起源、定义和流派,讨论了该技术在国内外的商用发展情况和产业链现状,分析了SDN技术的价值、未来发展趋势及大规模商用可能遇到的阻力,并提出了将其进行运营商全网应用的构想。

一起典型电压切换回路接线错误的原因分析

双母线接线方式中电压切换装置能够实现电压回路的自动切换,从而为保护、测量、计量提供正确的电压来源。文章结合现场运行中发生的一起电压切换回路接线错误,分析了电压切换装置的原理和重要性,有助于提高运维人员发现问题的能力和专业技能。

220kV线路双重化及操作机构箱配置不当的危害

针对220 k V线路保护装置或断路器改造中,进行保护双重化配置,以及操作继电器箱中防跃回路与断路器本体防跃回路共存时,引发的隐患问题进行介绍。同时阐述了双重化不合理配置将带来断路器拒动,进而起动母差失灵保护,造成母线所在机组停运、线路跳闸以及防跃回路共存将引起断路器在分断时卡涩进而引起拉弧,严重损坏断路器等一系列重大问题。

Microscopic shell-model description of irrotational-flow dynamics in^(102)Pd

The structure of the low-lying collective excitations in 102Pd is examined within the recently proposed microscopic shell-model version of the Bohr-Mottelson(BM)collective model.A good description of the excitation energies of the lowest ground,γ,andβbands,as well as the staggering function between the collective states of theγband and some other energy-dependent quantities,is obtained.The low-energy intraband and interband quadrupole dynamics is reasonably well described within the present proton-neutron sympletic based shell-model approach without the use of an effective charge and compared with the predictions of nuclear structure models.The obtained results of the present study shed light on the question of the existence of irrotational-flow type quadrupole dynamics,which lies on the ground of the original BM model of quantized vibrations and surface-wave rotations in atomic nuclei.

Shell-model representations of the microscopic version of the Bohr-Mottelson collective model

The structure of the irreducible collective spaces of the group Sp(12,R),which many-particle nuclear states are classified according to the chain Sp(12,R)⊃U(6)⊃SO(6)⊃SU_(pn)(3)⊗SO(2)⊃SO(3)of the proton-neutron symplectic model(PNSM),is considered in detail.This chain of the PNSM was recently shown to correspond to a microscopic shell-model version of the Bohr-Mottelson collective model.The construction of the relevant shell-model representations of the Sp(12,R)group along this chain is considered for three nuclei with varying collective properties and from different mass regions.It is shown that the SU_(pn)(3)basis states of the Sp(12,R)representations belonging to the SO(6)irreps with seniorityυ≥υ0,withυ0 denoting the maximal seniority SO(6)irrep contained in the Sp(12,R)bandhead,are always Pauli allowed,but organized in a different way into different SO(6)shells.This is in contrast to the case of filling the levels of the standard three-dimensional harmonic oscillator and using the plethysm operation.Although the SU_(pn)(3)multiplets withinυ<υ0 are not all Pauli forbidden,it is safe to discard them.The results obtained in the present work are important for the practical application of the microscopic version of the Bohr-Mottelson collective model.

Proton-neutron symplectic model description of ^(106)Cd

In this study,a microscopic shell-model description of the low-lying collective states in the weakly deformed nucleus ^(106)Cd within the recently proposed microscopic version of the Bohr-Mottelson model is provided.A good description of the excitation energies of the lowest ground,γ,andβquasibands is obtained without the adjustable kinetic energy term.Furthermore,γdegrees of freedom are shown to play a crucial role in the description of spectroscopy of this nucleus.A modified SU(3)SU(3)preserving high-order interaction is used to produce aγ-unstable type of odd-even staggering,observed experimentally between the states of the quasi-γband.The current approach enables the characterization of observed intraband and interband quadrupole collectivity.The findings of this study propose an alternative interpretation of the fundamental question regarding the nature of low-energy vibrations,as well as the emergence of deformation and collectivity in weakly deformed atomic nuclei.

On the microscopic shell-model version of the Bohr-Mottelson collective model

The recently proposed microscopic shell-model version of the Bohr-Mottelson(BM) collective model is considered in more detail in the coordinate representation.The latter possesses a clear and transparent physical meaning,which reveals several features of the new version of the collective model missed in the previous formulation.The relationship to the original BM model is considered,along with the relationships between the different limiting submodels of the microscopic version of the BM model,which closely resemble the relationships of the original Wilets-Jean and rotor models.The kinematically correct many-particle wave functions of the microscopic version of the BM model,conserving the experimentally observed integrals of motion,are shown to consist of collective irrotational-flow and intrinsic components-in accordance with the original BM unified model.The general BM Hamiltonian is obtained as a contraction limit of the microscopic many-particle nuclear Hamiltonian,or,alternatively,by restricting the latter to the scalar O(m) irreducible collective space.

红花水提取物的化学成分研究

目的对红花水提取物中的化学成分进行研究。方法通过大孔吸附树脂、Sephadex LH-20、反相HPLC等多种色谱进行分离纯化，根据理化性质和波谱数据进行鉴定。结果从红花水提物中分离得到了12个化合物，分别为（2E，8E）-12R—tetradeeadiene-4，6-diyne-1，12，14-triol（1），（8Z）-decaene-4，6-diyn-1—0-B-D—glucopyranoside（2），（8E）-decaene-4，6-diyn-1-0胡-D—glucopyranoside（3），（2S）-4’，5，6，7-四羟基二氢黄酮6-01B—D-吡喃葡萄糖苷（4），对羟基苯甲酸（5），异香草酸（6），dihydropha—seicacidmethylester．3-D移-D—glucoside（7），dihydrophaseicacidmethylester（8），对羟基苯甲醛（9），N，N＇-dicoumaroyl-putrescine（10），4-（4＇-羟基苯基）-丁-3-烯-2-酮（11），对羟基苯乙酮（12）。结论化合物1，3～4，6，8，10为首次从红花中分离得到，并首次报道化合物1的绝对构型及碳谱数据。

基于NMR指导的六神曲成分研究

目的研究六神曲的化学成分。方法运用各种色谱技术分离六神曲的化学成分,并利用理化性质和波谱技术鉴定其结构。结果从六神曲中分离得到11个成分,分别鉴定为5-二十五烷基间苯二酚(1)、5-十七烷基间苯二酚(2)、5-二十一烷基间苯二酚(3)、5-二十三烷基间苯二酚(4)、(9S,10E,12Z)-9-hydroxy-10,12-octadecadienoic acid(5)、trans-4-hydroxy-2-nonenoic acid(6)、9,10,11-trihydroxy-11(E)-octadecenoic acid(7)、(9S,12R,13S)-9,12,13-trihydroxy-10(E)-octadecenoic acid(8)、(9S,12S,13S)-9,12,13-trihydroxy-10(E)-octadecenoic acid(9)、(9S,12S,13S)-9,12,13-trihydroxy-10(E)-octadecenoic acid methyl ester(10)、9,12(Z)-octadecadienoic acid methyl ester(11)。结论化合物1~11均为首次从六神曲中分离得到。抗炎活性研究显示,六神曲氯仿提取物和化合物8均显示出良好的抗炎活性。

维药驱虫斑鸠菊HPLC-ELSD特征图谱和2个主要成分含量测定

目的:采用HPLC-ELSD法建立维药驱虫斑鸠菊药材的特征图谱,并同时测定2个主要成分的含量。方法:采用植化方法分离鉴定12R,13R-二羟基-9-十八碳烯酸和斑鸠菊酸作为对照品,采用Waters Xbridge C18色谱柱(250 mm×4.6 mm,5μm),0.05%三氟乙酸水溶液(A)-乙腈(B)为流动相,梯度洗脱(0~5 min,60%B;5~20 min,60%B→90%B;20~25 min,90%B→92.5%B),流速1.0 mL·min^-1;柱温30℃;ELSD漂移管60℃,载气为N2,流速1.6 L·min^-1。进样量10μL。结果:建立驱虫斑鸠菊特征图谱,标定5个特征峰,25 min内驱虫斑鸠菊的主要色谱峰能够达到完全分离,对15批药材中2个成分进行含量测定。12R,13R-二羟基-9-十八碳烯酸和斑鸠菊酸分别在0.150 8~3.016μg(r=0.999 3,n=6)和0.812 0~16.24μg(r=0.999 2,n=6)范围内线性关系良好,方法的平均回收率(n=6)分别为99.0%(RSD=1.9%)和98.5%(RSD=1.6%);15批驱虫斑鸠菊中上述2个成分含量分别为0.27~1.11%和2.36~6.34%。结论:所建立特征图谱专属性强,结合2个主要成分含量测定能够为驱虫斑鸠菊的质量控制提供科学依据。

Expansion of potential 1/r12 of atomic systems in hyper-spherical harmonics

The expansion coefficient C-\L\(D) Of Coulomb potential 1/r(12) of atomic system in hyper-spherical harmonics is derived and the explicit expression is given.