Many degenerative or pathological processes, such as aging, cancer and coronary heart disease, are related to reactive oxygen species (ROS) and radical-mediated reactions. We examined the effectiveness of aged garlic ...Many degenerative or pathological processes, such as aging, cancer and coronary heart disease, are related to reactive oxygen species (ROS) and radical-mediated reactions. We examined the effectiveness of aged garlic extract (AGE), a garlic preparation rich in water-soluble cysteinyl moieties, for protection of cells from ROS produced by 6-hydroxy-dopamine (6-OHDA) using human neuroblastoma SH-SY5Y cells. Concomitant treatment of cells with AGE (2 and 4 mg/ml) showed the dose-dependent protective effect on the cell death induced by 6-OHDA. In addition, the AGE treatment significantly suppressed the increase of ROS generation by 6-OHDA. Furthermore, the protective effect of AGE was accompanied by activation of the nuclear factor erythroid 2-related factor 2 (Nrf2)-antioxidant response element (ARE) pathway and the increase of mRNAs of heme oxygenase-1 and NAD(P)H quinone oxidoreductase 1. These two enzymes are important in the cellular antioxidant system. These results indicated that AGE protected cells from ROS damage by not only capturing ROS directly but also activating the cellular antioxidant system by stimulating antioxidant gene expression via the Nrf2-ARE pathway. The present study suggested that AGE may be useful for prevention and treatment of cell damage caused by ROS.展开更多
New zircon U-Pb dates obtained by laser ablation inductively coupled plasma mass spectrometry(LA-ICP-MS),whole-rock geochemical data and Sm-Nd and Rb-Sr isotopic data are presented for miarolitic alkaline granites,por...New zircon U-Pb dates obtained by laser ablation inductively coupled plasma mass spectrometry(LA-ICP-MS),whole-rock geochemical data and Sm-Nd and Rb-Sr isotopic data are presented for miarolitic alkaline granites,porphyritic syenite and rhyolites of the Nianzishan A-Type granitoid complex(NAGC)in the Great Xing'an Range-Songliao Basin in Northeast(NE)China.New crystallization ages of 112.95±0.93 and 114.1±1.71 Ma for granite and 118.6±0.51 Ma for porphyritic syenite were determined by high-precision LA-ICP-MS.The εNd(t)of the rocks range from +1.85 to+2.06,with Nd model ages(T(DM1))from 671 to 821 Ma,indicating that the NAGC originated from juvenile source rocks and exhibits geochemical characteristics of A1-and A-Type granite which formed in an extensional setting.We attribute the magmatism to regional extension and lithospheric thinning caused by the subduction of the western Pacific Plate about 120 to 100 Ma.展开更多
文摘Many degenerative or pathological processes, such as aging, cancer and coronary heart disease, are related to reactive oxygen species (ROS) and radical-mediated reactions. We examined the effectiveness of aged garlic extract (AGE), a garlic preparation rich in water-soluble cysteinyl moieties, for protection of cells from ROS produced by 6-hydroxy-dopamine (6-OHDA) using human neuroblastoma SH-SY5Y cells. Concomitant treatment of cells with AGE (2 and 4 mg/ml) showed the dose-dependent protective effect on the cell death induced by 6-OHDA. In addition, the AGE treatment significantly suppressed the increase of ROS generation by 6-OHDA. Furthermore, the protective effect of AGE was accompanied by activation of the nuclear factor erythroid 2-related factor 2 (Nrf2)-antioxidant response element (ARE) pathway and the increase of mRNAs of heme oxygenase-1 and NAD(P)H quinone oxidoreductase 1. These two enzymes are important in the cellular antioxidant system. These results indicated that AGE protected cells from ROS damage by not only capturing ROS directly but also activating the cellular antioxidant system by stimulating antioxidant gene expression via the Nrf2-ARE pathway. The present study suggested that AGE may be useful for prevention and treatment of cell damage caused by ROS.
基金funded by the China Geological Survey (Nos. DD20160123 (DD-16-049, D1522), DD20160346, 1212011121075, 212010911028, 12120114020901)the National Natural Science Foundation of China (No.NSFC40802020)
文摘New zircon U-Pb dates obtained by laser ablation inductively coupled plasma mass spectrometry(LA-ICP-MS),whole-rock geochemical data and Sm-Nd and Rb-Sr isotopic data are presented for miarolitic alkaline granites,porphyritic syenite and rhyolites of the Nianzishan A-Type granitoid complex(NAGC)in the Great Xing'an Range-Songliao Basin in Northeast(NE)China.New crystallization ages of 112.95±0.93 and 114.1±1.71 Ma for granite and 118.6±0.51 Ma for porphyritic syenite were determined by high-precision LA-ICP-MS.The εNd(t)of the rocks range from +1.85 to+2.06,with Nd model ages(T(DM1))from 671 to 821 Ma,indicating that the NAGC originated from juvenile source rocks and exhibits geochemical characteristics of A1-and A-Type granite which formed in an extensional setting.We attribute the magmatism to regional extension and lithospheric thinning caused by the subduction of the western Pacific Plate about 120 to 100 Ma.
