OBJECTIVE To explore the effect of photodynamic therapy with benzoporphyrin derivative monoacid ring A （BPD-MA） on the proliferation and apoptosis of human bladder cancer cells. METHODS Photosensitization of BPD-MA ...OBJECTIVE To explore the effect of photodynamic therapy with benzoporphyrin derivative monoacid ring A （BPD-MA） on the proliferation and apoptosis of human bladder cancer cells. METHODS Photosensitization of BPD-MA was activated with a red light laser （632.8 nm） delivered at 10 mw/cm^2 to give a total dose of 2.4 J/cm^2. Cellular proliferative activity was measured using the 3-（4,5.- dimethylethiazil-2-yl）-2,5-Diph3-eyl tetrazolium bromide （MFi-） assay and 3H-thymidine incorporation. Cell apoptosis was determined with flow cytometry analysis and the terminal deoxyuridine nicked-labeling （TUNEL） assay. RESULTS At 24 h post photodynamic treatment, photodynamic therapy significantly decreased cellular proliferative activity. The rate of apoptosis in BIU-87 cells 8 h after photodynamic treatment significantly increased up to 26.11± 2.59% as analyzed with flow cytometry. In situ labeling of DNA cleavage products with the terminal deoxyuridine nicked-labeling （TUNEL） assay reinforced these observations, BPD-MA-mediated photosensitization increased the number of TUNEL-positive cells compared to the controls. However, laser irradiation alone, BPD-MA alone and sham radiation did not affect cellular proliferative activity or apoptosis of the human bladder cancer BIU-87 cells. CONCLUSION Photodynamic therapy with BPD-MA significantly decreases cellular proliferative activity and enhances apoptosis. Therapy using this method might be a promising approach to treat patients with bladder cancer.展开更多
文摘OBJECTIVE To explore the effect of photodynamic therapy with benzoporphyrin derivative monoacid ring A （BPD-MA） on the proliferation and apoptosis of human bladder cancer cells. METHODS Photosensitization of BPD-MA was activated with a red light laser （632.8 nm） delivered at 10 mw/cm^2 to give a total dose of 2.4 J/cm^2. Cellular proliferative activity was measured using the 3-（4,5.- dimethylethiazil-2-yl）-2,5-Diph3-eyl tetrazolium bromide （MFi-） assay and 3H-thymidine incorporation. Cell apoptosis was determined with flow cytometry analysis and the terminal deoxyuridine nicked-labeling （TUNEL） assay. RESULTS At 24 h post photodynamic treatment, photodynamic therapy significantly decreased cellular proliferative activity. The rate of apoptosis in BIU-87 cells 8 h after photodynamic treatment significantly increased up to 26.11± 2.59% as analyzed with flow cytometry. In situ labeling of DNA cleavage products with the terminal deoxyuridine nicked-labeling （TUNEL） assay reinforced these observations, BPD-MA-mediated photosensitization increased the number of TUNEL-positive cells compared to the controls. However, laser irradiation alone, BPD-MA alone and sham radiation did not affect cellular proliferative activity or apoptosis of the human bladder cancer BIU-87 cells. CONCLUSION Photodynamic therapy with BPD-MA significantly decreases cellular proliferative activity and enhances apoptosis. Therapy using this method might be a promising approach to treat patients with bladder cancer.
