240 laying birds were procured and distributed randomly into four treatments and four replicate (15 birds each) which was fed one of the following experimental diets containing different levels of probiotics (Biomin I...240 laying birds were procured and distributed randomly into four treatments and four replicate (15 birds each) which was fed one of the following experimental diets containing different levels of probiotics (Biomin IMBO) for seven weeks. 1-Basel diet (control groups), 2-Basel diet + 250 g/t, 3-Basel diet +500 g/t, 4-Basel diet +750 g/t feed respectively. As results was revealed, feed efficiency were improved significantly throughout the production periods展开更多
Acidithiobacillus caldus is one of the dominant sulfur-oxidizing bacteria in bioleaching reactors. It plays the essential role in maintaining the high acidity and oxidation of reduced inorganic sulfur compounds during...Acidithiobacillus caldus is one of the dominant sulfur-oxidizing bacteria in bioleaching reactors. It plays the essential role in maintaining the high acidity and oxidation of reduced inorganic sulfur compounds during bioleaching process. In this report, the complete genome sequence of A. caldus SM-1 is presented. The genome is composed of one chromosome （2,932,225 bp） and four plasmids （pLAtcl, pLAtc2, pLAtc3, pLAtcm） and it is rich in repetitive sequences （accounting for 11% of the total genome）, which are often associated with transposable genetic elements. In particular, twelve copies of ISAtfe and thirty-seven copies of ISAtcl have been identified, suggesting that they are active transposons in the genome. A. caldus SM-1 encodes all enzymes for the central metabolism and the assimilation of carbon compounds, among which 29 proteins/enzymes were identifiable with proteomic tools. The SM-1 fixes CO2 via the classical Calvin-Bassham--Benson （CBB） cycle, and can operate complete Embden-Meyerhof pathway （EMP）, pentose phosphate pathway （PPP）, and gluconeogenesis. It has an incomplete tricarboxylic acid cycle （TCA）. Four putative transporters involved in carbohydrate uptake were identified. Taken together, the results suggested that SM-1 was able to assimilate carbohydrates and this was subsequently confirmed experimentally because addition of 1% glucose or sucrose in basic salt medium significantly increased the growth of SM-1. It was concluded that the complete genome of SM-1 provided fundamental data for further investigation of its physiology and genetics, in addition to the carbon metabolism revealed in this study.展开更多
In an era of electronics,recovering the precious metal such as gold from ever increasing piles of electronic-wastes and metal-ion infested soil has become one of the prime concerns for researchers worldwide.Biological...In an era of electronics,recovering the precious metal such as gold from ever increasing piles of electronic-wastes and metal-ion infested soil has become one of the prime concerns for researchers worldwide.Biological mining is an attractive,economical and nonhazardous to recover gold from the low-grade auriferous ore containing waste or soil.This review represents the recent major biological gold retrieval methods used to bio-mine gold.The biomining methods discussed in this review include,bioleaching,bio-oxidation,bio-precipitation,bio-flotation,bio-flocculation,bio-sorption,bio-reduction,bioelectrometallurgical technologies and bio accumulation.The mechanism of gold biorecovery by microbes is explained in detail to explore its intracellular mechanistic,which help it withstand high concentrations of gold without causing any fatal consequences.Major challenges and future opportunities associated with each method and how they will dictate the fate of gold bio-metallurgy from metal wastes or metal infested soil bioremediation in the coming future are also discussed.With the help of concurrent advancements in high-throughput technologies,the gold bio-exploratory methods will speed up our ways to ensure maximum gold retrieval out of such low-grade ores containing sources,while keeping the gold mining clean and more sustainable.展开更多
文摘240 laying birds were procured and distributed randomly into four treatments and four replicate (15 birds each) which was fed one of the following experimental diets containing different levels of probiotics (Biomin IMBO) for seven weeks. 1-Basel diet (control groups), 2-Basel diet + 250 g/t, 3-Basel diet +500 g/t, 4-Basel diet +750 g/t feed respectively. As results was revealed, feed efficiency were improved significantly throughout the production periods
基金supported by the European Commission under the Sixth Framework Programme for Research and Development in the frame of the research projects BioMinE(European project contract NMP1-CT-500329-1)Bioshale(European project contract NMP2-CT-2004 505710)The author is also grateful to the Royal Society(U.K.)for the provision of an Industrial Fellowship.
基金supported by the National Science Foundation of China(No.30870039)the National Basic Research Program of China(973 Program,No.2010CB630903)
文摘Acidithiobacillus caldus is one of the dominant sulfur-oxidizing bacteria in bioleaching reactors. It plays the essential role in maintaining the high acidity and oxidation of reduced inorganic sulfur compounds during bioleaching process. In this report, the complete genome sequence of A. caldus SM-1 is presented. The genome is composed of one chromosome （2,932,225 bp） and four plasmids （pLAtcl, pLAtc2, pLAtc3, pLAtcm） and it is rich in repetitive sequences （accounting for 11% of the total genome）, which are often associated with transposable genetic elements. In particular, twelve copies of ISAtfe and thirty-seven copies of ISAtcl have been identified, suggesting that they are active transposons in the genome. A. caldus SM-1 encodes all enzymes for the central metabolism and the assimilation of carbon compounds, among which 29 proteins/enzymes were identifiable with proteomic tools. The SM-1 fixes CO2 via the classical Calvin-Bassham--Benson （CBB） cycle, and can operate complete Embden-Meyerhof pathway （EMP）, pentose phosphate pathway （PPP）, and gluconeogenesis. It has an incomplete tricarboxylic acid cycle （TCA）. Four putative transporters involved in carbohydrate uptake were identified. Taken together, the results suggested that SM-1 was able to assimilate carbohydrates and this was subsequently confirmed experimentally because addition of 1% glucose or sucrose in basic salt medium significantly increased the growth of SM-1. It was concluded that the complete genome of SM-1 provided fundamental data for further investigation of its physiology and genetics, in addition to the carbon metabolism revealed in this study.
基金supported by the University Malaysia Pahang, Malaysiafinancial support provided by university under Research Grant No.RDU190332Ministry of Education of Malaysia under the research Grant of RDU 190121.
文摘In an era of electronics,recovering the precious metal such as gold from ever increasing piles of electronic-wastes and metal-ion infested soil has become one of the prime concerns for researchers worldwide.Biological mining is an attractive,economical and nonhazardous to recover gold from the low-grade auriferous ore containing waste or soil.This review represents the recent major biological gold retrieval methods used to bio-mine gold.The biomining methods discussed in this review include,bioleaching,bio-oxidation,bio-precipitation,bio-flotation,bio-flocculation,bio-sorption,bio-reduction,bioelectrometallurgical technologies and bio accumulation.The mechanism of gold biorecovery by microbes is explained in detail to explore its intracellular mechanistic,which help it withstand high concentrations of gold without causing any fatal consequences.Major challenges and future opportunities associated with each method and how they will dictate the fate of gold bio-metallurgy from metal wastes or metal infested soil bioremediation in the coming future are also discussed.With the help of concurrent advancements in high-throughput technologies,the gold bio-exploratory methods will speed up our ways to ensure maximum gold retrieval out of such low-grade ores containing sources,while keeping the gold mining clean and more sustainable.
