Ten cases of chronic fatigue syndrome (CFS) were treated for 4 weeks by acupuncture, Chinese herbs and diet precautions. Diagnosis of CFS was determined by conventional medicine criteria(CDC criteria for CFS), physica...Ten cases of chronic fatigue syndrome (CFS) were treated for 4 weeks by acupuncture, Chinese herbs and diet precautions. Diagnosis of CFS was determined by conventional medicine criteria(CDC criteria for CFS), physical examination, and routine laboratory tests. TCM diagnosis of CFS relied on Four Examination Methods and Eight Principal Patterns. They were diagnosed as Ganyu Qizhi (liver and qi stagnation) and Pixu Qiruo (spleen and qi deficiency). The treatment method is to disperse the liver and facilitate qi, rehabilitate the spleen and strengthen qi (Shugan Liqi, Jianpi Yiqi). The result of the treatment indicated that the total effective rate was 100% in terms of fatigue, difficulty concentrating, headache, sore throat, muscle pain, joint aches, feverishness, rapid pulse, insomnia, depression, irregular menses, abdominal cramps, and night sweats. The percentages of specific symptoms of CFS in the patients were comparable to the conventional medical record (Table 1).展开更多
To rapidly and economically obtain knowledge about adult stage Schistosoma japonicum (Chinese strain) expressed genes using expressed sequence tag (EST) Methods A directional cDNA library constructed from Schisto...To rapidly and economically obtain knowledge about adult stage Schistosoma japonicum (Chinese strain) expressed genes using expressed sequence tag (EST) Methods A directional cDNA library constructed from Schistosoma japonicum (Chinese strain ) adult stage RNA was used to generate expressed sequence tags(ESTs) These were compared against an EMBL parasites database and GENBANK database by BLASTn and BLASTx Results A total of 314 phage clones were randomly selected for generating expressed sequence tags(ESTs) From these clones, 132 EST quality sequence were obtained Among these EST quality sequences,113 ESTs were successfully submitted to the dbEST at GenBanK A total of 7 6% of these EST quality sequences were previously identified sequence of Schistosoma japonicum, while 4 5% were putatively identified sequences of Schistosoma japonicum A total of 23 5% of these EST quality sequences were putatively identified sequence of Schistosoma mansoni or other organisms 57 6% had no matches in the database and were classified as unknown sequences Most ESTs with the putative protein identified belonged to housekeeping proteins Information about several interesting genes was found Conclusion Partial cDNA sequencing to generate expressed sequence tags (ESTs) has the potential to rapidly and economically increase our knowledge about adult stage Schistosoma japonicum(Chinese strain) expressed genes展开更多
文摘Ten cases of chronic fatigue syndrome (CFS) were treated for 4 weeks by acupuncture, Chinese herbs and diet precautions. Diagnosis of CFS was determined by conventional medicine criteria(CDC criteria for CFS), physical examination, and routine laboratory tests. TCM diagnosis of CFS relied on Four Examination Methods and Eight Principal Patterns. They were diagnosed as Ganyu Qizhi (liver and qi stagnation) and Pixu Qiruo (spleen and qi deficiency). The treatment method is to disperse the liver and facilitate qi, rehabilitate the spleen and strengthen qi (Shugan Liqi, Jianpi Yiqi). The result of the treatment indicated that the total effective rate was 100% in terms of fatigue, difficulty concentrating, headache, sore throat, muscle pain, joint aches, feverishness, rapid pulse, insomnia, depression, irregular menses, abdominal cramps, and night sweats. The percentages of specific symptoms of CFS in the patients were comparable to the conventional medical record (Table 1).
基金ThisprojectwassupportedbyNationalNaturalScienceFoundationofChina (No 30 0 70 683 )
文摘To rapidly and economically obtain knowledge about adult stage Schistosoma japonicum (Chinese strain) expressed genes using expressed sequence tag (EST) Methods A directional cDNA library constructed from Schistosoma japonicum (Chinese strain ) adult stage RNA was used to generate expressed sequence tags(ESTs) These were compared against an EMBL parasites database and GENBANK database by BLASTn and BLASTx Results A total of 314 phage clones were randomly selected for generating expressed sequence tags(ESTs) From these clones, 132 EST quality sequence were obtained Among these EST quality sequences,113 ESTs were successfully submitted to the dbEST at GenBanK A total of 7 6% of these EST quality sequences were previously identified sequence of Schistosoma japonicum, while 4 5% were putatively identified sequences of Schistosoma japonicum A total of 23 5% of these EST quality sequences were putatively identified sequence of Schistosoma mansoni or other organisms 57 6% had no matches in the database and were classified as unknown sequences Most ESTs with the putative protein identified belonged to housekeeping proteins Information about several interesting genes was found Conclusion Partial cDNA sequencing to generate expressed sequence tags (ESTs) has the potential to rapidly and economically increase our knowledge about adult stage Schistosoma japonicum(Chinese strain) expressed genes
