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高通量微孔板DAMBO-P^H荧光检测一氧化氮 被引量:2
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作者 张晓玲 杨桥 马明 《分析科学学报》 CAS CSCD 北大核心 2009年第5期522-526,共5页
一氧化氮(NO)是生物体内的一种重要生物信号传导分子,广泛参与生物体内多种生理及病理过程。为建立快速高效、准确检测生物体释放NO的分析方法,本文选用高灵敏度、高选择性的NO特异性荧光探针8-(3,4-diaminophenyl)-2,6-bis(2-car-boxye... 一氧化氮(NO)是生物体内的一种重要生物信号传导分子,广泛参与生物体内多种生理及病理过程。为建立快速高效、准确检测生物体释放NO的分析方法,本文选用高灵敏度、高选择性的NO特异性荧光探针8-(3,4-diaminophenyl)-2,6-bis(2-car-boxyethyl)-4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene(DAM-BO-PH),激发波长和发射波长分别为520 nm和535 nm,以高通量微孔板(384孔)作为实验工具载体。该方法荧光强度与NO浓度在8.0×10-10~8.0×10-7mol.L-1范围内呈良好线性关系,R=0.9989,检出限为0.18 nmol.L-1,回收率为98%~102%。该方法应用于多种生物样品中释放NO的分析检测,结果令人满意。 展开更多
关键词 一氧化氮 dambo-PH 荧光检测 高通量微孔板
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Greenhouse Gas Emissions from Intermittently Flooded (Dambo) Rice under Different Tillage Practices in Chiota Smallholder Farming Area of Zimbabwe 被引量:2
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作者 George Nyamadzawo Menas Wuta +2 位作者 Ngonidzashe Chirinda Lizzie Mujuru Jeffrey Lynn Smith 《Atmospheric and Climate Sciences》 2013年第4期13-20,共8页
Agriculture is one of the biggest sources of greenhouse gases. Rice production has been identified as one of the major sources of greenhouse gases, especially methane. However, data on the contributions of rice toward... Agriculture is one of the biggest sources of greenhouse gases. Rice production has been identified as one of the major sources of greenhouse gases, especially methane. However, data on the contributions of rice towards greenhouse gas emissions in tropical Africa are limited. In Zimbabwe, as in most of Sub-Saharan Africa, there are very few studies that have explored greenhouse gas emissions from agricultural lands. This study reports the first dataset on greenhouse gas emissions from intermittently flooded rice paddies in Zimbabwe. The objective of this study was to quantify greenhouse gas emissions from dambo rice under different tillage treatments, which were conventional tillage, no tillage, tied ridges, tied fallows, and mulching. Average soil nitrous oxide emissions were 5.9, 0.2, 5.4, 5.2 and 7.8 μg·m-2·hr-1 for tied fallows, conventional tillage, tied ridges, mulching and no tillage respectively. Average methane emission was 0.35 mg·m-2·hr-1 and maximum as 1.62 mg·m-2·hr-1. Average methane emissions for the different tillage systems were 0.20, 0.18, 0.45, 0.52 and 0.38 mg·m-2·hr-1 for tied fallows, conventional tillage, tied ridges, mulching and no tillage respectively. Carbon dioxide emissions were 98.1, 56.0, 69.9, 94.8 and 95.5 mg·m-2·hr-1 for tied fallows, conventional tillage, tied ridges, mulching and no tillage respectively. The estimated emissions per 150 day cropping season were 1.4, 3.6 and 0.6 kg·ha-1 for methane, carbon dioxide and nitrous oxide respectively. We concluded that intermittently saturated dambo rice Paddys are a potential source of greenhouse gases which is important to global greenhouse gas budgets, thus, they deserve more careful study. 展开更多
关键词 GREENHOUSE Gases SMALLHOLDER FARMING Sector RICE Seasonal WETLAND (dambo)
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Influence of Grazing Intensity on Soil Properties and Shaping Herbaceous Plant Communities in Semi-Arid Dambo Wetlands of Zimbabwe 被引量:3
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作者 E. Dahwa C. P. Mudzengi +4 位作者 T. Hungwe M. D. Shoko X. Poshiwa S. Kativu C. Murungweni 《Journal of Environmental Protection》 2013年第10期1181-1188,共8页
Key issues of concern regarding the environmental impacts of livestock on grazing land are their effects on soil, water quality, and biodiversity. This study was carried out to determine how grazing intensity influenc... Key issues of concern regarding the environmental impacts of livestock on grazing land are their effects on soil, water quality, and biodiversity. This study was carried out to determine how grazing intensity influences soil physical and chemical properties and occurrence of herbaceous plant species in dambo wetlands. Three categories of grazing intensity were selected from communal, small scale commercial and large scale commercial land. Dambos from the large scale commercial land functioned as the control. Data analysis included ANOVA and multivariate tests from CANOCO. There were significantly negative changes to soil nutrient status in communal dambos though with a higher number of rare taxa. Sodium, phosphorous, pH and infiltration rate were significant determinants of plant species occurrence. Overgrazing is threatening the productivity, stability, and ecological functioning of dambo soils in communal Zimbabwe. These dambos also require special conservation and management priorities as they contain a large number of rare plant species. 展开更多
关键词 dambo WETLANDS GRAZING Soil NUTRIENTS
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The Effect of Catena Position on Greenhouse Gas Emissions from Dambo Located Termite (<i>Odontotermes transvaalensis</i>) Mounds from Central Zimbabwe
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作者 George Nyamadzawo Jephita Gotosa +4 位作者 Justice Muvengwi Menas Wuta Justice Nyamangara Philip Nyamugafata Jeff L. Smith 《Atmospheric and Climate Sciences》 2012年第4期501-509,共9页
Methane (CH4), carbon dioxide (CO2) and nitrous oxide (N2)O) are greenhouse gases (GHGs) which cause global warming. Natural sources of GHGs include wetlands and termites. Previous studies have quantified GHG emission... Methane (CH4), carbon dioxide (CO2) and nitrous oxide (N2)O) are greenhouse gases (GHGs) which cause global warming. Natural sources of GHGs include wetlands and termites. Previous studies have quantified GHG emissions from upland termites and no study has reported GHG emissions from seasonal wetlands (dambo) located termite mounds. The objective of this study was to evaluate the effect of dambo catena position on termite mound distribution and GHG emissions. It was hypothesized that mound density and GHG emissions from Odontotermes transvaalensis mounds, vary with catena position. The evaluated catena positions were margin, mid-slope, lower slope and bottom. Mound density was significantly lower in the bottom when compared to the other catena positions. The mean GHG fluxes were 88 μg m2 hr-1, 0.78 mg m2 hr-1 and 1361 mg m2 hr-1 for N2) O, CH4 and CO2 respectively. Fluxes varied with catena position and were 0.48, 0.72, 1.35 and 0.79 mg m-2 hr-1 for CH4 , and 1173.7, 1440.7, 1798.7 and 922.8 mg m-2 hr-1 for CO2 in the margin, mid-slope, lower slope and the bottom catena position respectively. For N2) O, there were no significant differences between catena positions. It was concluded that dambo located Odontotermes transvaalensis termite mounds are an important source of GHGs, and emissions varied with catena position for CO2 and CH4. 展开更多
关键词 Greenhouse Gas Emissions TERMITES MOUNDS Odontotermes transvaalensis dambos CATENA POSITION
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中国福建省亚热带山地4000年来植被变化的孢粉记录 被引量:9
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作者 邱宏烈 《亚热带资源与环境学报》 2006年第1X期11-23,共13页
根据采自福建省中部戴云山脉亚高山水系源头地带(大致25°40’N,118°11’E)泥炭沉积洼地的孢粉纪录,对该地区亚热带山地植被近4000年来的变化进行了研究.孢粉记录和其他沉积资料显示,3个取样点的泥炭沉积开始于4000年前... 根据采自福建省中部戴云山脉亚高山水系源头地带(大致25°40’N,118°11’E)泥炭沉积洼地的孢粉纪录,对该地区亚热带山地植被近4000年来的变化进行了研究.孢粉记录和其他沉积资料显示,3个取样点的泥炭沉积开始于4000年前,很可能是全新世后期气候转冷的结果.在距今4000~1200年前期间,戴云山脉上部(海拔1300~1600m)的植被为亚热带针阔叶混交类型,优势种属包括Cryptomeria(柳杉)、Castanopsis(栲)、Quercus(栎)和Tsuga(铁杉)等.距今1200年前左右,该区域的植被发生了一次大的变化,表现在上述优势属种的孢粉在样品总孢粉数的比例突然下降,而Pinus(松),Gramineae(禾本科)以及Dicranopteris(芒萁)等属种的比例则提高了.这次剧烈的植被变化在其中的一个泥炭钻孔中留下了一层粘土记录,它代表了当时流域内植被受破坏后出现的严重土壤侵蚀.从泥炭沉积提取的孢粉记录还可以看出,研究区内广泛分布的松林是一种次生植被,它是人类不断干扰的结果. 展开更多
关键词 戴云山脉 孢粉集合 泥炭沼泽 人为干扰 泥炭沉积洼地
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