[目的]研究苦参碱在温度诱导的EOPO/无机盐体系中的分配行为。[方法]利用紫外分光光度计对苦参碱进行定性定量测量。利用双水相分配技术研究不同的EOPO的分子量(SDP28、SDP30,SDP35)、不同种类的盐(KH2PO4、K2HPO4.3H2O、Na2SO4、NH4C l...[目的]研究苦参碱在温度诱导的EOPO/无机盐体系中的分配行为。[方法]利用紫外分光光度计对苦参碱进行定性定量测量。利用双水相分配技术研究不同的EOPO的分子量(SDP28、SDP30,SDP35)、不同种类的盐(KH2PO4、K2HPO4.3H2O、Na2SO4、NH4C l)对双水相分配的影响,并利用正交试验确定最佳的分离纯化的条件。[结果]苦参碱在EOPO 3种分子量和3种盐温度诱导双水相的分配特性研究表明,EOPO分子量为SDP28时,最大收率为77.59%,盐为K2HPO4时,最大收率为77.59%,分离效果最好。L9(33)正交试验研究表明,最佳的分离条件为:K2HPO4用量2 g;水量10 m l;SDP28用量15 m l时,最大收率为86.39%。[结论]盐的种类对双水相的分配行为影响最大。展开更多
The partition coefficients of baicalin were measured in ethylene oxide and propylene oxide (EOPO)/salt aqueous two-phase systems at 298.15K. It was found that most of baicalin partitioned into EOPO-rich phase. The par...The partition coefficients of baicalin were measured in ethylene oxide and propylene oxide (EOPO)/salt aqueous two-phase systems at 298.15K. It was found that most of baicalin partitioned into EOPO-rich phase. The partition coefficients of baicalin varied from 10 to 120. The effect of various factors, including tie-line length, salt composition, molecular weight of EOPO, and solution pH, on the partition behavior was investigated in EOPO/salt systems. Furthermore the partition coefficients of baicalin were correlated using the modified Diamond-Hsu model. Good agreement with experimental data is obtained. The average relative deviations are less than 5.0%.展开更多
A series of ethylene oxide (EO)-propylene oxide (PO) randomco-polymers (EOPO) were used to form aqueous two-phase systems (ATPS)with ammonium sulfate. Effects of EOPO's properties on the phaseseparation behaviors ...A series of ethylene oxide (EO)-propylene oxide (PO) randomco-polymers (EOPO) were used to form aqueous two-phase systems (ATPS)with ammonium sulfate. Effects of EOPO's properties on the phaseseparation behaviors and on the partition of cephalexin and7-aminodesacetoxicephalosporanic acid (7-ADCA) in ATPS wereinvestigated. Both the molar mass and molar ratio of EO to PO of EOPOcould greatly influence partition behaviors of cephalexin and 7-ADCAas well as the binodal curve of ATPS.展开更多
文摘[目的]研究苦参碱在温度诱导的EOPO/无机盐体系中的分配行为。[方法]利用紫外分光光度计对苦参碱进行定性定量测量。利用双水相分配技术研究不同的EOPO的分子量(SDP28、SDP30,SDP35)、不同种类的盐(KH2PO4、K2HPO4.3H2O、Na2SO4、NH4C l)对双水相分配的影响,并利用正交试验确定最佳的分离纯化的条件。[结果]苦参碱在EOPO 3种分子量和3种盐温度诱导双水相的分配特性研究表明,EOPO分子量为SDP28时,最大收率为77.59%,盐为K2HPO4时,最大收率为77.59%,分离效果最好。L9(33)正交试验研究表明,最佳的分离条件为:K2HPO4用量2 g;水量10 m l;SDP28用量15 m l时,最大收率为86.39%。[结论]盐的种类对双水相的分配行为影响最大。
基金Supported by the National Natural Science Foundation of China (No. 29736180).
文摘The partition coefficients of baicalin were measured in ethylene oxide and propylene oxide (EOPO)/salt aqueous two-phase systems at 298.15K. It was found that most of baicalin partitioned into EOPO-rich phase. The partition coefficients of baicalin varied from 10 to 120. The effect of various factors, including tie-line length, salt composition, molecular weight of EOPO, and solution pH, on the partition behavior was investigated in EOPO/salt systems. Furthermore the partition coefficients of baicalin were correlated using the modified Diamond-Hsu model. Good agreement with experimental data is obtained. The average relative deviations are less than 5.0%.
基金Supported by the National Natural Science Foundation of China (No. 29873014) and the Key Disciplinary Foundation of Shanghai.
文摘A series of ethylene oxide (EO)-propylene oxide (PO) randomco-polymers (EOPO) were used to form aqueous two-phase systems (ATPS)with ammonium sulfate. Effects of EOPO's properties on the phaseseparation behaviors and on the partition of cephalexin and7-aminodesacetoxicephalosporanic acid (7-ADCA) in ATPS wereinvestigated. Both the molar mass and molar ratio of EO to PO of EOPOcould greatly influence partition behaviors of cephalexin and 7-ADCAas well as the binodal curve of ATPS.
