青铜小单孢菌(Micromonospora chalcea, M. chalcea)FIM 02-523能够合成对乏氧肿瘤细胞、艰难梭菌等具有活性的环脂肽类化合物rakicidins。利用Illumina HiSeq高通量测序平台,本研究首次对M. chalcea FIM 02-523进行全基因组测序,得到...青铜小单孢菌(Micromonospora chalcea, M. chalcea)FIM 02-523能够合成对乏氧肿瘤细胞、艰难梭菌等具有活性的环脂肽类化合物rakicidins。利用Illumina HiSeq高通量测序平台,本研究首次对M. chalcea FIM 02-523进行全基因组测序,得到总长约6.74Mb的序列信息。分析表明基因组GC含量为72.89%,包含了6167个蛋白编码序列。利用AntiSMASH预测基因组中存在19个生物合成基因簇。结合PKS/NRPS生物合成特征和rakicidins化学结构特点,定位到了rakicidins的生物合成基因簇,并初步推测其生物合成途径。研究为M. chalcea FIM 02-523的功能基因组学研究和代谢调控提供了理论基础。展开更多
By using AP-FIM the varity of the ordered degree of Ni_3Al with L1_2 structure with B content was studied. The possibility of boron improving ductility was also discussed from bonding between Ni and Al atoms, and anti...By using AP-FIM the varity of the ordered degree of Ni_3Al with L1_2 structure with B content was studied. The possibility of boron improving ductility was also discussed from bonding between Ni and Al atoms, and antisite defects in Ni_3Al. The extent of ordering is reduced with an increase in boron content and the autisite defects are most obvious for 0.52at.K B-doped sample that has the best ductility. Some results were verified by X-ray diffraction non. The addition of boron not only influences electron environment at grain boundary but also in the interior of Ni_3Al gm ins, the latter is favorable to improve the ductility of Ni_3Al grains.展开更多
文摘青铜小单孢菌(Micromonospora chalcea, M. chalcea)FIM 02-523能够合成对乏氧肿瘤细胞、艰难梭菌等具有活性的环脂肽类化合物rakicidins。利用Illumina HiSeq高通量测序平台,本研究首次对M. chalcea FIM 02-523进行全基因组测序,得到总长约6.74Mb的序列信息。分析表明基因组GC含量为72.89%,包含了6167个蛋白编码序列。利用AntiSMASH预测基因组中存在19个生物合成基因簇。结合PKS/NRPS生物合成特征和rakicidins化学结构特点,定位到了rakicidins的生物合成基因簇,并初步推测其生物合成途径。研究为M. chalcea FIM 02-523的功能基因组学研究和代谢调控提供了理论基础。
基金This project was supported by the National Nature Science Foundation of China!(Grant No.59831020 and 59895156)
文摘By using AP-FIM the varity of the ordered degree of Ni_3Al with L1_2 structure with B content was studied. The possibility of boron improving ductility was also discussed from bonding between Ni and Al atoms, and antisite defects in Ni_3Al. The extent of ordering is reduced with an increase in boron content and the autisite defects are most obvious for 0.52at.K B-doped sample that has the best ductility. Some results were verified by X-ray diffraction non. The addition of boron not only influences electron environment at grain boundary but also in the interior of Ni_3Al gm ins, the latter is favorable to improve the ductility of Ni_3Al grains.
