Colorectal cancer(CRC)ranks third in the number of cancers mainly because of the inability to diagnose it at an early stage.The pathogenesis of CRC is complicated,which is the result of the complex interaction of mult...Colorectal cancer(CRC)ranks third in the number of cancers mainly because of the inability to diagnose it at an early stage.The pathogenesis of CRC is complicated,which is the result of the complex interaction of multiple genetic and environmental factors.Currently,one of the main treatments for CRC is chemotherapy.But the primary cause of CRC treatment failure is drug resistance.The expression of cyclin-dependent kinase 9(CDK9)was correlated with elevated autophagy levels in colon cancer,and high expression of CDK9 indicates a poor prognosis in CRC.The incidence of autophagy and the expressions of Beclin 1 and ATP binding cassette transporter G2 are different in left and right colon cancer,and autophagy may be involved in the occurrence of chemotherapy resistance.In this article,the roles of CDK9,ATP binding cassette transporter G2 and Beclin 1 in CRC were elucidated,emphasizing the linkages among them and providing potential therapeutic targets of CRC.展开更多
1.27μm波段的氧分子近红外气辉是火星大气最重要的气辉辐射之一,该气辉高光谱分辨辐射传输模型的建立对于研制火星探测载荷,反演火星大气的风场温度场与臭氧浓度,以及研究火星空间物理,有重要的科学价值与工程意义.在研究火星大气O_(2)...1.27μm波段的氧分子近红外气辉是火星大气最重要的气辉辐射之一,该气辉高光谱分辨辐射传输模型的建立对于研制火星探测载荷,反演火星大气的风场温度场与臭氧浓度,以及研究火星空间物理,有重要的科学价值与工程意义.在研究火星大气O_(2)(a^(1)Δ_(g))气辉光化学反应模型的基础上,提出了O_(2)(a^(1)Δ_(g))气辉体辐射率的计算方法,并建立了火星大气气辉辐射传输理论;通过与用于研究火星大气特征的光谱学探测仪(Spectroscopy Spectrograph for the Investigation of Characteristics of the Atmosphere of Mars,SPICAM)的实测数据进行对比,验证了所建立的火星O_(2)(a^(1)Δ_(g))气辉高光谱分辨辐射传输模型的准确性;针对火星与地球大气的O_(2)(a^(1)Δ_(g))气辉,在体辐射率、自吸收效应,以及临边辐射光谱特性三个方面进行了系统深入的比较,对比结果表明,火星大气由于密度低、氧气丰度小,其自吸收效应可以忽略不计,但其O_(2)(a^(1)Δ_(g))气辉辐射强度与地球大气相当,可以用于火星大气的风场温度场与臭氧浓度的探测与反演.展开更多
BACKGROUND A series of long non-coding RNAs(lncRNAs)have been reported to play a crucial role in cancer biology.Some previous studies report that lncRNA CDKN2B-AS1 is involved in some human malignancies.However,its ro...BACKGROUND A series of long non-coding RNAs(lncRNAs)have been reported to play a crucial role in cancer biology.Some previous studies report that lncRNA CDKN2B-AS1 is involved in some human malignancies.However,its role in hepatocellular carcinoma(HCC)has not been fully deciphered.AIM To decipher the role of CDKN2B-AS1 in the progression of HCC.METHODS CDKN2B-AS1 expression in HCC was detected by quantitative real-time polymerase chain reaction.The malignant phenotypes of Li-7 and SNU-182 cells were detected by the CCK-8 method,EdU method,and flow cytometry,respectively.RNA immunoprecipitation was executed to confirm the interaction between CDKN2B-AS1 and E2F transcription factor 1(E2F1).Luciferase reporter assay and chromatin immunoprecipitation were performed to verify the binding of E2F1 to the promoter of G protein subunit alpha Z(GNAZ).E2F1 and GNAZ were detected by western blot in HCC cells.RESULTS In HCC tissues,CDKN2B-AS1 was upregulated.Depletion of CDKN2B-AS1 inhibited the proliferation of HCC cells,and the depletion of CDKN2B-AS1 also induced cell cycle arrest and apoptosis.CDKN2B-AS1 could interact with E2F1.Depletion of CDKN2B-AS1 inhibited the binding of E2F1 to the GNAZ promoter region.Overexpression of E2F1 reversed the biological effects of depletion of CDKN2B-AS1 on the malignant behaviors of HCC cells.CONCLUSION CDKN2B-AS1 recruits E2F1 to facilitate GNAZ transcription to promote HCC progression.展开更多
基金Supported by the National Natural Science Foundation of China,No.82272996the Science and Technology Program of Guangzhou,No.202206010081.
文摘Colorectal cancer(CRC)ranks third in the number of cancers mainly because of the inability to diagnose it at an early stage.The pathogenesis of CRC is complicated,which is the result of the complex interaction of multiple genetic and environmental factors.Currently,one of the main treatments for CRC is chemotherapy.But the primary cause of CRC treatment failure is drug resistance.The expression of cyclin-dependent kinase 9(CDK9)was correlated with elevated autophagy levels in colon cancer,and high expression of CDK9 indicates a poor prognosis in CRC.The incidence of autophagy and the expressions of Beclin 1 and ATP binding cassette transporter G2 are different in left and right colon cancer,and autophagy may be involved in the occurrence of chemotherapy resistance.In this article,the roles of CDK9,ATP binding cassette transporter G2 and Beclin 1 in CRC were elucidated,emphasizing the linkages among them and providing potential therapeutic targets of CRC.
文摘1.27μm波段的氧分子近红外气辉是火星大气最重要的气辉辐射之一,该气辉高光谱分辨辐射传输模型的建立对于研制火星探测载荷,反演火星大气的风场温度场与臭氧浓度,以及研究火星空间物理,有重要的科学价值与工程意义.在研究火星大气O_(2)(a^(1)Δ_(g))气辉光化学反应模型的基础上,提出了O_(2)(a^(1)Δ_(g))气辉体辐射率的计算方法,并建立了火星大气气辉辐射传输理论;通过与用于研究火星大气特征的光谱学探测仪(Spectroscopy Spectrograph for the Investigation of Characteristics of the Atmosphere of Mars,SPICAM)的实测数据进行对比,验证了所建立的火星O_(2)(a^(1)Δ_(g))气辉高光谱分辨辐射传输模型的准确性;针对火星与地球大气的O_(2)(a^(1)Δ_(g))气辉,在体辐射率、自吸收效应,以及临边辐射光谱特性三个方面进行了系统深入的比较,对比结果表明,火星大气由于密度低、氧气丰度小,其自吸收效应可以忽略不计,但其O_(2)(a^(1)Δ_(g))气辉辐射强度与地球大气相当,可以用于火星大气的风场温度场与臭氧浓度的探测与反演.
文摘BACKGROUND A series of long non-coding RNAs(lncRNAs)have been reported to play a crucial role in cancer biology.Some previous studies report that lncRNA CDKN2B-AS1 is involved in some human malignancies.However,its role in hepatocellular carcinoma(HCC)has not been fully deciphered.AIM To decipher the role of CDKN2B-AS1 in the progression of HCC.METHODS CDKN2B-AS1 expression in HCC was detected by quantitative real-time polymerase chain reaction.The malignant phenotypes of Li-7 and SNU-182 cells were detected by the CCK-8 method,EdU method,and flow cytometry,respectively.RNA immunoprecipitation was executed to confirm the interaction between CDKN2B-AS1 and E2F transcription factor 1(E2F1).Luciferase reporter assay and chromatin immunoprecipitation were performed to verify the binding of E2F1 to the promoter of G protein subunit alpha Z(GNAZ).E2F1 and GNAZ were detected by western blot in HCC cells.RESULTS In HCC tissues,CDKN2B-AS1 was upregulated.Depletion of CDKN2B-AS1 inhibited the proliferation of HCC cells,and the depletion of CDKN2B-AS1 also induced cell cycle arrest and apoptosis.CDKN2B-AS1 could interact with E2F1.Depletion of CDKN2B-AS1 inhibited the binding of E2F1 to the GNAZ promoter region.Overexpression of E2F1 reversed the biological effects of depletion of CDKN2B-AS1 on the malignant behaviors of HCC cells.CONCLUSION CDKN2B-AS1 recruits E2F1 to facilitate GNAZ transcription to promote HCC progression.
