A reserved-phase HPLC method was developed for the determination of barbaloin in Aloe vera L. var. chinensis (Haw.) Berger and Aloe barbadensis Miller, and whether there was a close relationship between the contents o...A reserved-phase HPLC method was developed for the determination of barbaloin in Aloe vera L. var. chinensis (Haw.) Berger and Aloe barbadensis Miller, and whether there was a close relationship between the contents of barbaloin and their environments in which they were growing was decided. A Hypersil ODS column (4.6 mm×200 mm, 5 μm)was used with a mobile phase of methanol-water (40:60, containing 0.1% acetic acid), the flow rate being 1.0 mL·min -1, detection wavelength at 359 nm, and the column temperature being 30℃. The linear range of barbaloin was between 0.0726 and 0.726 μg with a correlation coefficient of 0.9998 and the regression equation being Y=1.9202×10 6X-1801.9. Barbaloin was stable in methanol in 48 h and the instrument precision was 1.2% while the method precision was 4.9%. The contents of barbaloin of 12 samples ranged from 6.160 to 319.1 μg·g -1. The method developed was fast and simple with good reproducibility. There was high correlation between the contents of barbaloin and their growing environments.展开更多
该文采用微波辅助法提取米邦塔仙人掌多糖,并与热回流提取方法进行比较。通过设计正交试验,得出优化的工艺条件:以水为提取剂,料液比1∶10(w/v),提取2次,每次提取3 m in,微波炉功率700 W,选用70%乙醇沉淀。微波提取粗多糖的得率和含量...该文采用微波辅助法提取米邦塔仙人掌多糖,并与热回流提取方法进行比较。通过设计正交试验,得出优化的工艺条件:以水为提取剂,料液比1∶10(w/v),提取2次,每次提取3 m in,微波炉功率700 W,选用70%乙醇沉淀。微波提取粗多糖的得率和含量分别为6.8%和8.5%,高于热回流提取粗多糖的得率4.7%和含量8.3%。微波提取不仅缩短了提取时间,降低提取剂用量,而且提高了仙人掌多糖得率。展开更多
目的分离、纯化具有降血糖作用的仙人掌多糖组分。方法经热水提取、乙醇沉淀、DEAE Sepharose fast flow离子交换色谱和Sephadex G系列凝胶滤过色谱纯化得到5种仙人掌多糖组分。醋酸纤维薄膜电泳检测多糖纯度,凝胶色谱测定相对分子质量...目的分离、纯化具有降血糖作用的仙人掌多糖组分。方法经热水提取、乙醇沉淀、DEAE Sepharose fast flow离子交换色谱和Sephadex G系列凝胶滤过色谱纯化得到5种仙人掌多糖组分。醋酸纤维薄膜电泳检测多糖纯度,凝胶色谱测定相对分子质量,高效液相色谱测定糖的组成。结果5种多糖都已达到电泳纯。它们的相对分子质量依次为2.0×103、1.0×106、4.0×103、9.2×105、5.0×103。ODP1由鼠李糖组成,ODP2可能由鼠李糖和葡萄糖组成,ODP4可能由鼠李糖和D-半乳糖组成。ODP3和ODP5分别由鼠李糖和一未知糖组分组成。结论仙人掌多糖的提取没有必要采用脱蛋白、脱色素步骤,本实验所提取的仙人掌多糖是均一的。展开更多
目的:比较采用充填器与HAWE牙体颈部成型片对楔状缺损牙进行光固化复合树脂充填成型治疗后充填体边缘微渗漏情况。方法:取新鲜离体双尖牙40颗,在颊侧颈部釉牙骨质界处制备标准V类(3mm×2mm×2mm)洞型,随机分成2组,A组采用不锈...目的:比较采用充填器与HAWE牙体颈部成型片对楔状缺损牙进行光固化复合树脂充填成型治疗后充填体边缘微渗漏情况。方法:取新鲜离体双尖牙40颗,在颊侧颈部釉牙骨质界处制备标准V类(3mm×2mm×2mm)洞型,随机分成2组,A组采用不锈钢两头扁充填器,B组采用颈部成型片行复合树脂充填治疗。样本经冷热循环后,在2%亚甲基蓝溶液中染色24h,将充填体正中颊舌向纵行剖开,立体显微镜下观察修复体边缘染色情况,采用Image-Pro plus 6.0图像分析软件测量微渗漏深度。结果:两种方法均有微渗漏现象发生,A组边缘微渗漏深度为(0.641±0.370)mm,B组边缘微渗漏深度为(0.395±0.462)mm。两种方法树脂充填后边缘微渗漏程度及深度差异均有统计学意义(P<0.05)。结论:虽然两种方法均不能消除充填体边缘微渗漏,但用颈部成型片的边缘封闭性明显优于传统充填器,而且操作简单,方便,节省时间,适合临床推广。展开更多
文摘A reserved-phase HPLC method was developed for the determination of barbaloin in Aloe vera L. var. chinensis (Haw.) Berger and Aloe barbadensis Miller, and whether there was a close relationship between the contents of barbaloin and their environments in which they were growing was decided. A Hypersil ODS column (4.6 mm×200 mm, 5 μm)was used with a mobile phase of methanol-water (40:60, containing 0.1% acetic acid), the flow rate being 1.0 mL·min -1, detection wavelength at 359 nm, and the column temperature being 30℃. The linear range of barbaloin was between 0.0726 and 0.726 μg with a correlation coefficient of 0.9998 and the regression equation being Y=1.9202×10 6X-1801.9. Barbaloin was stable in methanol in 48 h and the instrument precision was 1.2% while the method precision was 4.9%. The contents of barbaloin of 12 samples ranged from 6.160 to 319.1 μg·g -1. The method developed was fast and simple with good reproducibility. There was high correlation between the contents of barbaloin and their growing environments.
文摘该文采用微波辅助法提取米邦塔仙人掌多糖,并与热回流提取方法进行比较。通过设计正交试验,得出优化的工艺条件:以水为提取剂,料液比1∶10(w/v),提取2次,每次提取3 m in,微波炉功率700 W,选用70%乙醇沉淀。微波提取粗多糖的得率和含量分别为6.8%和8.5%,高于热回流提取粗多糖的得率4.7%和含量8.3%。微波提取不仅缩短了提取时间,降低提取剂用量,而且提高了仙人掌多糖得率。
文摘目的分离、纯化具有降血糖作用的仙人掌多糖组分。方法经热水提取、乙醇沉淀、DEAE Sepharose fast flow离子交换色谱和Sephadex G系列凝胶滤过色谱纯化得到5种仙人掌多糖组分。醋酸纤维薄膜电泳检测多糖纯度,凝胶色谱测定相对分子质量,高效液相色谱测定糖的组成。结果5种多糖都已达到电泳纯。它们的相对分子质量依次为2.0×103、1.0×106、4.0×103、9.2×105、5.0×103。ODP1由鼠李糖组成,ODP2可能由鼠李糖和葡萄糖组成,ODP4可能由鼠李糖和D-半乳糖组成。ODP3和ODP5分别由鼠李糖和一未知糖组分组成。结论仙人掌多糖的提取没有必要采用脱蛋白、脱色素步骤,本实验所提取的仙人掌多糖是均一的。
文摘目的:比较采用充填器与HAWE牙体颈部成型片对楔状缺损牙进行光固化复合树脂充填成型治疗后充填体边缘微渗漏情况。方法:取新鲜离体双尖牙40颗,在颊侧颈部釉牙骨质界处制备标准V类(3mm×2mm×2mm)洞型,随机分成2组,A组采用不锈钢两头扁充填器,B组采用颈部成型片行复合树脂充填治疗。样本经冷热循环后,在2%亚甲基蓝溶液中染色24h,将充填体正中颊舌向纵行剖开,立体显微镜下观察修复体边缘染色情况,采用Image-Pro plus 6.0图像分析软件测量微渗漏深度。结果:两种方法均有微渗漏现象发生,A组边缘微渗漏深度为(0.641±0.370)mm,B组边缘微渗漏深度为(0.395±0.462)mm。两种方法树脂充填后边缘微渗漏程度及深度差异均有统计学意义(P<0.05)。结论:虽然两种方法均不能消除充填体边缘微渗漏,但用颈部成型片的边缘封闭性明显优于传统充填器,而且操作简单,方便,节省时间,适合临床推广。
