该工作以富含大量胸腺嘧啶（Thymine，T）核酸单链为识别分子，SYBR Green I （SG）为荧光基团，建立了一种简单、灵敏的荧光增强法检测Hg2＋。由于T-Hg2＋-T键的形成，富T单链自我折叠或者两两配对形成双链DNA结构，当溶液中的SG嵌入DN...该工作以富含大量胸腺嘧啶（Thymine，T）核酸单链为识别分子，SYBR Green I （SG）为荧光基团，建立了一种简单、灵敏的荧光增强法检测Hg2＋。由于T-Hg2＋-T键的形成，富T单链自我折叠或者两两配对形成双链DNA结构，当溶液中的SG嵌入DNA双链中时，SG荧光强度显著增强。实验结果表明，SG荧光强度随着Hg2＋浓度的增加而增加。在最优实验条件下，SG的荧光强度与Hg2＋的浓度在4．000×10-7~2．000×10-6 mol/L范围内呈线性关系，检出限为3．900×10-8 mol/L。该方法在含5．0％湘江水实际样品中获得的回收率为98．72％~104．5％，因此该传感器可用于实际湘江水样品中Hg2＋的测量。展开更多
[Objective] This work was aimed to explore the mechanism of Hg2+ toxicity on plants.[Method]Activities of peroxidase(POD),catalase(CAT)and superoxide dismutase(SOD)were investigated in wheat(Triticum aestivum L.)seedl...[Objective] This work was aimed to explore the mechanism of Hg2+ toxicity on plants.[Method]Activities of peroxidase(POD),catalase(CAT)and superoxide dismutase(SOD)were investigated in wheat(Triticum aestivum L.)seedlings under Hg2+ stress at different concentrations.[Result]① There were no obvious effects on the growth of seedlings when the concentration of Hg2+ was lower than 0.10 mmol/L.However,toxic effects on the growth of seedling were observed when the concentration of Hg2+ was higher than 0.10 mmol/L.② Different tissues showed different resistant ability in response to Hg2+ stress.The leaves and roots of wheat seedlings were more insensitive to Hg2+ toxicity.③ CAT was more sensitive to Hg2+ stress compared to POD and SOD.[Conclusion]The toxic effect was related to the concentration of Hg2+(0.10 mmol/L).The higher concentration of Hg2+ could affect the expression of POD,CAT,and SOD isozymes in the leaves,roots of wheat seedlings and germinated seeds,which further affect the normal metabolism of membrane lipid and inhibit the growth of wheat seedlings at last.展开更多
在湿法烟气脱硫系统中由于还原性物质如亚硫酸根离子的存在使得被捕集的Hg2+又以Hg0的形态排出,导致湿法烟气脱硫(wet flue gas desulfurization,WFGD)系统的脱汞效率降低。该文利用鼓泡反应器,研究了SO32-浓度、SO32-/HSO32-比值、浆...在湿法烟气脱硫系统中由于还原性物质如亚硫酸根离子的存在使得被捕集的Hg2+又以Hg0的形态排出,导致湿法烟气脱硫(wet flue gas desulfurization,WFGD)系统的脱汞效率降低。该文利用鼓泡反应器,研究了SO32-浓度、SO32-/HSO32-比值、浆液pH值、反应温度、Cl-浓度对湿法烟气脱硫浆液中Hg2+还原和Hg0再释放特性的影响。实验结果表明,Hg0再释放速率随着浆液中S(IV)浓度的降低、pH值降低、SO32-/HSO32-比值的增大、反应温度的升高而增大。通过提高浆液pH值、增加S(IV)浓度、降低SO32-/HSO32-比值、降低反应温度可以抑制或者减缓湿法烟气脱硫浆液中Hg0再释放,为WFGD同时控制煤燃烧过程中的汞奠定应用基础。展开更多
文摘该工作以富含大量胸腺嘧啶（Thymine，T）核酸单链为识别分子，SYBR Green I （SG）为荧光基团，建立了一种简单、灵敏的荧光增强法检测Hg2＋。由于T-Hg2＋-T键的形成，富T单链自我折叠或者两两配对形成双链DNA结构，当溶液中的SG嵌入DNA双链中时，SG荧光强度显著增强。实验结果表明，SG荧光强度随着Hg2＋浓度的增加而增加。在最优实验条件下，SG的荧光强度与Hg2＋的浓度在4．000×10-7~2．000×10-6 mol/L范围内呈线性关系，检出限为3．900×10-8 mol/L。该方法在含5．0％湘江水实际样品中获得的回收率为98．72％~104．5％，因此该传感器可用于实际湘江水样品中Hg2＋的测量。
文摘[Objective] This work was aimed to explore the mechanism of Hg2+ toxicity on plants.[Method]Activities of peroxidase(POD),catalase(CAT)and superoxide dismutase(SOD)were investigated in wheat(Triticum aestivum L.)seedlings under Hg2+ stress at different concentrations.[Result]① There were no obvious effects on the growth of seedlings when the concentration of Hg2+ was lower than 0.10 mmol/L.However,toxic effects on the growth of seedling were observed when the concentration of Hg2+ was higher than 0.10 mmol/L.② Different tissues showed different resistant ability in response to Hg2+ stress.The leaves and roots of wheat seedlings were more insensitive to Hg2+ toxicity.③ CAT was more sensitive to Hg2+ stress compared to POD and SOD.[Conclusion]The toxic effect was related to the concentration of Hg2+(0.10 mmol/L).The higher concentration of Hg2+ could affect the expression of POD,CAT,and SOD isozymes in the leaves,roots of wheat seedlings and germinated seeds,which further affect the normal metabolism of membrane lipid and inhibit the growth of wheat seedlings at last.
文摘在湿法烟气脱硫系统中由于还原性物质如亚硫酸根离子的存在使得被捕集的Hg2+又以Hg0的形态排出,导致湿法烟气脱硫(wet flue gas desulfurization,WFGD)系统的脱汞效率降低。该文利用鼓泡反应器,研究了SO32-浓度、SO32-/HSO32-比值、浆液pH值、反应温度、Cl-浓度对湿法烟气脱硫浆液中Hg2+还原和Hg0再释放特性的影响。实验结果表明,Hg0再释放速率随着浆液中S(IV)浓度的降低、pH值降低、SO32-/HSO32-比值的增大、反应温度的升高而增大。通过提高浆液pH值、增加S(IV)浓度、降低SO32-/HSO32-比值、降低反应温度可以抑制或者减缓湿法烟气脱硫浆液中Hg0再释放,为WFGD同时控制煤燃烧过程中的汞奠定应用基础。
