Monitoring Passive Transfer of Immunity in Neonatal Calves by Measuring Levels of IgG in Blood Using Immunoassay Method and Refractometer Measures of Serum and Colostrum

IgG Check calf test has been used in this study to identify if a failure of passive transfer occurs in neonatal calves by measuring the level of IgG in blood. An adequate level of IgG measured in all calves at 48 hours, 7 days and 14 days after birth showed level of IgG 1000 mg/dl. This level indicated that they have good passive transfer of immunity. Also, in this study colostrum quality fed to calves was detected using Brix Refractometer. The colostrum is of high quality as it contains 50 mg/ml of IgG. This Brix refractometer can be used on the farm level to estimate colostrum IgG content and monitoring colostrum feeding practices. Colostrum with high quality IgG could provide calves with enough IgG to attain successful passive transfer of immunity. Brix and Obione refractometers provide simple, rapid method for estimating IgG concentration on calf serum, thus considered to be the most common method for determining passive transfer failure.

基于基因转录起始过程的四物汤抗乳腺癌活性成分群辨识

目的:辨识四物汤的抗乳腺癌活性成分。方法:利用基于基因转录起始过程的中药活性成分辨识方法(ITPI):采用全基因组芯片检测技术识别差异表达基因(DEGs),DEGs的转录因子(TFs)来源于GeneCards,在数据库STITCH中查找能与转录因子相互作用的化学成分,利用四物汤化学成分与转录因子相互作用的化学成分之间的二维结构相似性辨识四物汤的有效成分群。结果:辨识出了52个影响基因表达的活性成分、52个活性成分作用的20个差异基因,并提供了活性成分与转录因子、差异基因间的对应关系。结论:本研究为四物汤抗乳腺癌活性成分群辨识提供了有效方法,将为在四物汤活性成分的基础上研发成分明确、机制清晰的抗乳腺癌药物奠定基础。