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反场箍缩装置KTX真空室结构稳定性分析 被引量:4
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作者 史善爽 宋云涛 +4 位作者 杨庆喜 徐皓 刘万东 万树德 潘洪涛 《真空科学与技术学报》 EI CAS CSCD 北大核心 2014年第10期1087-1090,共4页
KTX为中国科学技术大学在建的反场箍缩实验装置,在同类装置之间,其参数介于美国MST和意大利的RFX之间。KTX真空室壁厚仅为6 mm,属于非标准环形薄壳结构,其结构的稳定性分析是确定结构设计是否合理的关键因素。本文利用有限元的方法,对KT... KTX为中国科学技术大学在建的反场箍缩实验装置,在同类装置之间,其参数介于美国MST和意大利的RFX之间。KTX真空室壁厚仅为6 mm,属于非标准环形薄壳结构,其结构的稳定性分析是确定结构设计是否合理的关键因素。本文利用有限元的方法,对KTX真空室结构进行了线性和非线性屈曲分析。得到了两种情形下应力和变形加载曲线。通过对计算结果的对比和分析,验证了目前真空室结构的稳定性符合要求,并具有一定的安全裕度。 展开更多
关键词 ktx 真空室 屈曲分析 有限元
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KTX反场箍缩装置纵场线圈结构设计与电磁分析 被引量:1
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作者 郑金星 宋云涛 +6 位作者 杨庆喜 徐薇薇 刘旭峰 刘万东 丁卫星 张建成 史善爽 《原子能科学技术》 EI CAS CSCD 北大核心 2013年第11期2166-2171,共6页
KTX反场箍缩装置的主要参数介于RFX装置与MST装置之间。反场箍缩的外加纵场需跟随等离子体电流的演化而变化,同时由于RFP中的磁面对于外部特别是等离子体边界处的径向磁场较敏感,所以需外部线圈的磁场更加精细,这对于线圈的磁场分布、... KTX反场箍缩装置的主要参数介于RFX装置与MST装置之间。反场箍缩的外加纵场需跟随等离子体电流的演化而变化,同时由于RFP中的磁面对于外部特别是等离子体边界处的径向磁场较敏感,所以需外部线圈的磁场更加精细,这对于线圈的磁场分布、误差场以及波纹度等的设计提出了更高的要求。根据KTX物理目标参数要求,提出矩形和楔形截面纵场磁体线圈设计方案,借助有限元软件和程序分析了其电磁场空间分布和结构受力大小。结果表明,6.4°楔形截面方案相比矩形截面方案在控制误差场方面更具有可行性。 展开更多
关键词 ktx 纵场磁体线圈 RFP 结构设计 电磁分析
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少棘蜈蚣钾通道毒素多肽KTX-Ssm175的表达、纯化与鉴定 被引量:1
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作者 尹世金 陈璇 +7 位作者 闻闫瀚 陆春兰 胡青兰 邹艳 张丰 王冠 黄鑫 黄谨 《中南民族大学学报(自然科学版)》 CAS 北大核心 2016年第4期43-47,共5页
采用Illumina II代转录组测序技术构建了湖北少棘蜈蚣毒腺转录组数据库,从中筛选到一个全新的毒素多肽编码基因KTX-Ssm175.经序列比对显示KTX-Ssm175多肽与κ-SLPTX-Ssm1a高度同源,提示KTX-Ssm175多肽为一种新的电压门控性钾通道阻断剂... 采用Illumina II代转录组测序技术构建了湖北少棘蜈蚣毒腺转录组数据库,从中筛选到一个全新的毒素多肽编码基因KTX-Ssm175.经序列比对显示KTX-Ssm175多肽与κ-SLPTX-Ssm1a高度同源,提示KTX-Ssm175多肽为一种新的电压门控性钾通道阻断剂.采用基因工程技术构建了p GEX-4T-1-KTX-Ssm175原核表达载体,通过GST融合蛋白表达法对KTX-Ssm175多肽在大肠杆菌Rosetta中进行诱导表达和分离纯化.MALDI-TOF-MS质谱测定显示纯化的KTX-Ssm175多肽分子量与理论值相吻合,说明成功实现了KTX-Ssm175多肽的基因工程制备,为深入研究其结构与功能提供了物质基础. 展开更多
关键词 少棘蜈蚣 转录组 ktx-Ssm175 钾通道阻断剂 基因工程
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形象差异与传统融合——韩国KTX高速列车造型及装饰设计 被引量:2
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作者 王玮 王喆 《设计艺术研究》 2014年第2期43-49,共7页
为了探索高速列车造型及装饰设计理论与实践,对韩国KTX高速列车造型及装饰设计特点进行研究。韩国在引进以法国TGV高速列车为代表的高精尖技术时,通过形象差异化战略,对KTX列车头型予以适当地改造,基于空气动力学进行优化设计,使之适应... 为了探索高速列车造型及装饰设计理论与实践,对韩国KTX高速列车造型及装饰设计特点进行研究。韩国在引进以法国TGV高速列车为代表的高精尖技术时,通过形象差异化战略,对KTX列车头型予以适当地改造,基于空气动力学进行优化设计,使之适应当地的具体情况。此外,韩国还以象思维为视角,创造了一种"有意味的形式"的子弹头列车。同时,韩国进一步借鉴"观物取象"的设计思想,以"象"作为设计灵感的源泉,通过车体内外装饰设计与韩国自身的传统文化、风土人情相结合,成功打上了本土烙印,并实现了异质文化共生,迅速建立起高速铁路技术输入国家特立独行的品牌形象,值得我们参考和借鉴。 展开更多
关键词 韩国ktx高速列车 形象差异化 传统融合 造型及装饰设计
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KTX反场箍缩装置主支撑结构设计与分析 被引量:2
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作者 张建成 宋云涛 +4 位作者 杨庆喜 赵文龙 郑金星 丁卫星 刘万东 《原子能科学技术》 EI CAS CSCD 北大核心 2013年第10期1862-1866,共5页
KTX反场箍缩实验装置主支撑由柔性支撑和支撑平台组成,主要用来支撑真空室、导体壳和纵场线圈的重量。鉴于主支撑结构的重要性,通过有限元分析和理论分析相结合的方法对主支撑进行了强度分析和稳定性分析,给出了主支撑应力分布和变形情... KTX反场箍缩实验装置主支撑由柔性支撑和支撑平台组成,主要用来支撑真空室、导体壳和纵场线圈的重量。鉴于主支撑结构的重要性,通过有限元分析和理论分析相结合的方法对主支撑进行了强度分析和稳定性分析,给出了主支撑应力分布和变形情况及不同结构参数下的临界载荷。分析结果验证了主支撑结构的可行性,并为主支撑的后续优化设计提供理论依据。 展开更多
关键词 ktx 柔性支撑 屈曲 有限元分析
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KTX真空室支撑焊接设计及有限元分析 被引量:1
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作者 汪震 杨庆喜 +3 位作者 宋云涛 徐皓 万树德 刘万东 《核聚变与等离子体物理》 CAS CSCD 北大核心 2015年第3期240-245,共6页
提出了一种KTX真空室支撑焊接方案,并利用有限元方法模拟计算了焊接温度场分布及焊接残余应力场分布。通过理论分析和有限元分析相结合的方法,对焊缝结构进行了强度分析。分析结果验证了焊接结构的可靠性,并为真空室支撑结构的焊接工艺... 提出了一种KTX真空室支撑焊接方案,并利用有限元方法模拟计算了焊接温度场分布及焊接残余应力场分布。通过理论分析和有限元分析相结合的方法,对焊缝结构进行了强度分析。分析结果验证了焊接结构的可靠性,并为真空室支撑结构的焊接工艺提供了理论依据。 展开更多
关键词 ktx 有限元分析 焊接温度场 残余应力 强度校核
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KTX反场箍缩装置装配基准网的创建与分析
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作者 付宸硕 刘万东 +4 位作者 杨庆喜 李弘 徐永兴 顾永奇 张平 《核聚变与等离子体物理》 CAS CSCD 北大核心 2016年第3期260-265,共6页
介绍了反场箍缩KTX装置主机主要构成部件、安装精度要求以及装配方案。通过建立一个独立于装置之外且可永久保存的装配基准网,以及对坐标系控制网的测量数据分析,确保各部件的安装定位和准确测量。
关键词 ktx 装配 基准网 测量系统
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KTX真空室支撑系统强度分析及优化设计
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作者 汪震 宋云涛 +5 位作者 杨庆喜 史善爽 徐皓 万树德 李宏 刘万东 《核聚变与等离子体物理》 CAS CSCD 北大核心 2014年第4期367-373,共7页
对反场箍缩核聚变实验装置KTX真空室支撑结构强度进行理论分析,利用有限元方法进行了分析计算,并且根据分析结果,对支撑柱、螺栓组和支撑环板进行了优化。理论计算和有限元分析的结果均证明了真空室支撑系统的可靠性,同时优化结果也为KT... 对反场箍缩核聚变实验装置KTX真空室支撑结构强度进行理论分析,利用有限元方法进行了分析计算,并且根据分析结果,对支撑柱、螺栓组和支撑环板进行了优化。理论计算和有限元分析的结果均证明了真空室支撑系统的可靠性,同时优化结果也为KTX真空室支撑系统的实施提供技术参考。 展开更多
关键词 ktx 真空室支撑系统 有限元分析 结构优化
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Verification and Optimization of Vacuum Vessel Supports Design in KTX 被引量:1
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作者 罗志仁 杨庆喜 +8 位作者 宋云涛 杜双松 徐皓 李宏 刘万东 王忠伟 张善文 胡欢 张建武 《Plasma Science and Technology》 SCIE EI CAS CSCD 2015年第3期241-246,共6页
The Vacuum Vessel (VV) system is an essential component of Keda Torus for eX- periment (KTX), and various scenarios might take place on it. The VV's supports should be adequately strong to stand against various l... The Vacuum Vessel (VV) system is an essential component of Keda Torus for eX- periment (KTX), and various scenarios might take place on it. The VV's supports should be adequately strong to stand against various loads on VV, which might happen in extreme scenarios. Therefore, the design of VV supports is verified in a single extreme scenario and is subsequently optimized in this report. The numerical simulation based on Finite Element theory is performed as the major method for analysis and optimization. The electromagnetic force in previous analyses serves as the load for the mechanical analyses of supports. During the optimization, the stresses of the VV supports decrease remarkably after introducing cotters. Finally, the optimum design has been worked out. It satisfies the requirements regarding the strength and convenience in assembly. 展开更多
关键词 ktx VV supports numerical simulation OPTIMIZATION
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Design of the poloidal field system for KTX 被引量:1
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作者 Wei YOU Hong LI +14 位作者 Wenzhe MAO Wei BAI Cui TU Bing LUO Zichao LI Yolbarsop ADIL Jintong HU Bingjia XIAO Qingxi YANG Jinlin XIE Tao LAN Adi LIU Weixing DING Chijin XIAO Wandong LIU 《Plasma Science and Technology》 SCIE EI CAS CSCD 2018年第11期126-134,共9页
The design of the poloidal field (PF) system includes the ohmic heating field system and the equilibrium (EQ) field system, and is the basis for the design of a magnetic confinement fusion device. A coupling betwe... The design of the poloidal field (PF) system includes the ohmic heating field system and the equilibrium (EQ) field system, and is the basis for the design of a magnetic confinement fusion device. A coupling between the poloidal and plasma currents, especially the eddy current in the stabilizing shell, yields design difficulties. The effects of the eddy current in the stabilizing shell on the poloidal magnetic field also cannot be ignored. A new PF system design is thus proposed. By using a low-μ material (μ = 0.001, ε = 1) instead of a conductive shell, an electromagnetic model is established that can provide a continuous eddy current distribution on the conductive shell. In this model, a 3D time-domain problem with shells translates into a 2D magnetostatic problem, and the accuracy of the calculation is improved. Based on these current distributions, we design the PF system and analyze how the EQ coils and conductive shell affect the plasma EQ when the plasma ramps up. To meet the mainframe design requirements and achieve an efficient power-supply design, the position and connection of the poloidal coils are optimized further. 展开更多
关键词 RFP ktx poloidal field system electromagnetic design
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The Design of the Plasma Control System in KTX 被引量:1
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作者 章勇 肖炳甲 +6 位作者 袁旗平 张睿瑞 杨飞 李实 李弘 刘阿娣 刘万东 《Plasma Science and Technology》 SCIE EI CAS CSCD 2015年第4期340-346,共7页
KTX(Keda Torus for eXperiment)is a new reversed field pinch device.The KTX plasma control system(PCS)can provide real-time,stable,flexible plasma control which is designed by ASIPP(Institute of Plasma Physics,Chi... KTX(Keda Torus for eXperiment)is a new reversed field pinch device.The KTX plasma control system(PCS)can provide real-time,stable,flexible plasma control which is designed by ASIPP(Institute of Plasma Physics,Chinese Academy of Sciences),based on the Linux cluster system and EPICS(Experimental Physics and Industrial Control System)framework,and developed from DIII-D(Doublet III-D)PCS.The control of the equilibrium field in KTX uses a PID(Proportional-Integral-Derivative)feedback controller.The control of the gas injection is an open loop control.The plasma control simulation system is one part of the plasma control system,which is used to test the plasma control algorithm if it is revised and updated.The KTX PCS has been successfully tested using HT-7(Hefei Torus 7)experiment data in simulation mode.In the next phase,an error field feedback control and KTX simulator will be added to the KTX PCS,and the KTX PCS will be applied in experiments in the future. 展开更多
关键词 ktx plasma control EPICS Linux cluster
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Analysis of an Extreme Scenario in the Vacuum Vessel in KTX
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作者 罗志仁 杨庆喜 +7 位作者 宋云涛 李宏 刘万东 杜双松 徐皓 王忠伟 姚尧 胡欢 《Plasma Science and Technology》 SCIE EI CAS CSCD 2015年第6期510-516,共7页
The Vacuum Vessel (VV) system is a vital component of Keda Torus for experiment (KTX). Various accidental scenarios might occur on the VV. In this report, an extreme scenario is assumed and studied: plasma accide... The Vacuum Vessel (VV) system is a vital component of Keda Torus for experiment (KTX). Various accidental scenarios might occur on the VV. In this report, an extreme scenario is assumed and studied: plasma accidental termination during the fiat-top stage. Numerical simulations based on finite element are performed as the major tool for aualyses. The detailed distributions of eddy and the reaction forces on VV are extracted, and the total eddy current and the maximum reaction force due to electromagnetic load are figured out. In addition, according to the results, the VV can be approximately regarded as a centrally symmetric structure, even though its ports distribution is asymmetric. 展开更多
关键词 ktx VV extreme scenario nulnerical sinmlation EDDY reaction force
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Design of a Wedge-Shaped Toroidal Field Winding for KTX Device
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作者 郑金星 宋云涛 +4 位作者 杨庆喜 刘万东 丁卫星 刘旭峰 杨雷 《Plasma Science and Technology》 SCIE EI CAS CSCD 2014年第9期878-884,共7页
The KTX device is a reversed field pinch (RFP) device currently under construction. Its maximum plasma current is designed as 1 MA with a discharge time longer than 100 ms. Its major radius is 1.4 m and its minor ra... The KTX device is a reversed field pinch (RFP) device currently under construction. Its maximum plasma current is designed as 1 MA with a discharge time longer than 100 ms. Its major radius is 1.4 m and its minor radius is 0.55 m. One of the most important problems in the magnet system design is how to reduce the TF magnetic field ripple and error field. A new wedge- shaped TF coil is put forward for the KTX device and its electromagnetic properties are compared with those of rectangular-shaped TF coils. The error field B,I/Bt of wedge-shaped TF coils with 6.4 degrees is about 6% as compared with 8% in the case of a rectangular-shaped TF coil. Besides, the wedge-shaped TF coils have a lower magnetic field ripple at the edge of the plasma region, which is smaller than 7.5% at R=1.83 m and 2% at R=l.07 m. This means that the tokamak operation mode may be feasible for this device when the plasma area becomes smaller, because the maximum ripple in the plasma area of the tokamak model is always required to be smaller than 0.4%. Detailed analysis of the results shows that the structure of the wedged-shape TF coil is reliable. It can serve as a reference for TF coil design of small aspect ratio RFPs or similar torus devices. 展开更多
关键词 ktx toroidal field winding wedge-shaped winding MAGNET
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Capability Assessment of the Equilibrium Field System in KTX
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作者 罗兵 罗正平 +10 位作者 肖炳甲 尤玮 谭名昇 郭勇 白伟 毛文哲 李弘 刘阿娣 兰涛 谢锦林 刘万东 《Plasma Science and Technology》 SCIE EI CAS CSCD 2016年第1期90-96,共7页
Radial equilibrium of the KTX plasma column is maintained by the vertical field which is produced by the equilibrium field coils.The equilibrium is also affected by the eddy current,which is generated by the coupling ... Radial equilibrium of the KTX plasma column is maintained by the vertical field which is produced by the equilibrium field coils.The equilibrium is also affected by the eddy current,which is generated by the coupling of copper shell,plasma and poloidal field coils.An equivalent circuit model is developed to analyze the dynamic performance of equilibrium field coils,without auxiliary power input to equilibrium field coils and passive conductors.Considering the coupling of poloidal field coils,copper shell and plasma,the evolution of spatial distribution of the eddy current density on the copper shell is estimated by finite element to analyze the effect of shell to balance.The simulation results show that the copper shell and equilibrium field coils can provide enough vertical field to balance 1 MA plasma current in phase 1 of a KTX discharge.Auxiliary power supply on the EQ coils is necessary to control the horizontal displacement of KTX due to the finite resistance effect of the shell. 展开更多
关键词 ktx plasma equilibrium circuit model eddy current
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轮胎型真空室在核聚变装置上的设计与分析研究
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作者 徐皓 杨庆喜 +4 位作者 宋云涛 史善爽 张平 李弘 刘万东 《真空科学与技术学报》 EI CAS CSCD 北大核心 2018年第2期94-101,共8页
在磁约束核聚变装置中,真空室是一个非常重要的关键部件,它为高温等离子体提供洁净运行环境。KTX装置是一种反场箍缩核聚变装置,它有别于托卡马克装置和仿星器核聚变装置。目前核聚变装置上使用的真空室由多个虾米扇段通过焊接成环形真... 在磁约束核聚变装置中,真空室是一个非常重要的关键部件,它为高温等离子体提供洁净运行环境。KTX装置是一种反场箍缩核聚变装置,它有别于托卡马克装置和仿星器核聚变装置。目前核聚变装置上使用的真空室由多个虾米扇段通过焊接成环形真空室,此类型真空室焊缝数量多,焊缝距离长,焊接变形控制难度大。根据装置要求,KTX磁约束核聚变装置真空室设计为轮胎型结构,大半径为1.4 m,截面半径为0.4 m,真空度为1.5×10-6Pa,且具有易于进入维护和更换真空室内部部件功能。本论文针对KTX装置真空室设计要求和技术参数,对轮胎型真空室开展设计,在设计过程中,基于真空室各种运行工况,对真空室开展了热、电磁和结构相关方面分析计算,根据计算结果对真空室进行优化和验证。本类型真空室设计和分析方法为未来磁约束核聚变装置真空室设计研制提供参考和借鉴。 展开更多
关键词 ktx核聚变装置 轮胎型真空室 有限元方法 屈曲分析 电磁分析
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隧道断层开挖引起的地下水系统变化分析(英文) 被引量:1
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作者 Jung-Wook Park Chung-In Lee 《重庆交通大学学报(自然科学版)》 CAS 北大核心 2011年第A02期1073-1079,共7页
在岩层中开挖隧道时以及开挖后,地下水会倒灌进隧道中。利用SEEP/W程序,对倒灌进隧道的地下水的水量及水位变化进行了二维有限元分析。研究对象是位于韩国Gyeongnam市Cheonseong山区的Wonhyo隧道,隧道模型范围内既有湿地,也有断裂带,选... 在岩层中开挖隧道时以及开挖后,地下水会倒灌进隧道中。利用SEEP/W程序,对倒灌进隧道的地下水的水量及水位变化进行了二维有限元分析。研究对象是位于韩国Gyeongnam市Cheonseong山区的Wonhyo隧道,隧道模型范围内既有湿地,也有断裂带,选取了隧道沿途的4个典型区域进行研究。通过DFN模型计算了岩层中的导水率的各向异性度,再将各项异性度数据输入连续性模型。为最大限度地减少水利特性所造成的不确定性,文中进行了参数分析。此外,为了反映某一介质在饱和和非饱和条件下对水的储存和运输能力,模型中还引入了体积含水量和水利传导性。 展开更多
关键词 环境影响评价 韩国高速列车 二维有限元分析 离散裂隙网络模型
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Construction of an Hα diagnostic system and its application to determine neutral hydrogen densities on the Keda Torus eXperiment
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作者 Junfeng Zhu Tao Lan +18 位作者 Ge Zhuang Tijian Deng Jie Wu Hangqi Xu Chen Chen Sen Zhang Jiaren Wu Yiming Zu Hong Li Jinlin Xie Ahdi Liu Zixi Liu Zhengwei Wu Hai Wang Xiaohui Wen Haiyang Zhou Chijin Xiao Weixing Ding Wandong Liu 《Chinese Physics B》 SCIE EI CAS CSCD 2019年第10期428-434,共7页
A 10-channel Hα diagnostic system has been designed with the rapid response rate of 300 kHz, spatial resolution of about 40 mm, and overlap between adjacent channels of about 3%, and it has been implemented successfu... A 10-channel Hα diagnostic system has been designed with the rapid response rate of 300 kHz, spatial resolution of about 40 mm, and overlap between adjacent channels of about 3%, and it has been implemented successfully on Keda Torus eXperiment(KTX), a newly constructed, reversed field pinch(RFP) experimental device at the University of Science and Technology of China(USTC). This diagnostic system is a very important tool for the initial KTX operations. It is compact,with an aperture slit replacing the traditional optical lens system. A flexural interference filter is designed to prevent the center wavelength from shifting too much as the increase of angle from vertical incidence. To eliminate the stray light,the interior of the system is covered with the black aluminum foil having a very high absorptivity. Using the Hαemission data, together with the profiles of electron temperature and density obtained from the Langmuir probe, the neutral density profiles have been calculated for KTX plasmas. The rapid response rate and good spatial resolution of this Hαdiagnostic system will be beneficial for many studies in RFP plasma physics. 展开更多
关键词 DIAGNOSTIC NEUTRAL density Keda TORUS eXperiment(ktx) device
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Overview of Kidney Transplantation: A Single Centre Report from China
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作者 Kiran Jang Kunwar Li Heng +1 位作者 Fuqing Zeng Zhendi Wang 《Open Journal of Organ Transplant Surgery》 2016年第2期7-11,共5页
Introduction: Worldwide, End Stage Renal Disease (ESRD) is one of the leading disease with prolong morbidity. Kidney transplantation offers the best solution for the problem. The shortage of donor kidney is even bigge... Introduction: Worldwide, End Stage Renal Disease (ESRD) is one of the leading disease with prolong morbidity. Kidney transplantation offers the best solution for the problem. The shortage of donor kidney is even bigger problem due to transplantation being one of the routine procedures. The use of deceased donor definitely increases the pool of donor with excellent immediate and long-term follow-up proven results. Aim: The aim is to analyze and summarize the outcome of Kidney transplantation. Methods & Materials: A total of 78 cases of Kidney Transplantation were selected for the study and categorized as: Group I—41 (living Donor), Group II—23 (DCD) & Group III—15 (DBD). Perspective study was done with clean data recorded & maintained pre-operatively, post-operatively and follow-up from Jan 2011 to Dec 2015 in our hospital. Post-operative graft status, complications and at least 1-year follow-up were area of main focus. Results: All patients underwent successful kidney transplantation. In Group I, the number of living donor kidney transplantation is 41 whereas in Group II (DCD) & III (DBD), the number of deceased donor transplantation is 23 and 15 respectively. The Normal functioning of graft (NGF) was 38 (87.8%), 16 (69.6%) & 11 (73.3%) in Group I, II & III respectively along with Poor Graft function (PGF) in Group I—4 (9.7%), II—5 (21.7%) & III—2 (13.3%) managed by continuing dialysis. Delayed graft function (DGF) was noted I-1 (2.4%), II-2 (8.6%) & III-1 (6.6%) in respective group, which returned to normal function post intervention. Therefore, 1<sup>st</sup> year graft survival was >93% [(Group I (97.6%), Group II (95.6%) & Group III (93.3%) respectively]. Manageable surgical complication were found in Group I—8 (19.5%), Group II—5 (21.7%) & Group III—2 (13.3%) like hematoma, hydronephrosis, leakage except one emboli related nephrectomy of transplanted kidney & one pneumonia led death in Group II. The overall survival was greater than 90% [(Group I (97.6%), Group II (91.3%) & Group III (93.3%) respectively] in all three groups after at least 1-year follow-up study, which was an excellent prognosis. Conclusion: Kidney Transplantation is safe, effective and the best method of treatment for ESRD. Significant improvement in quality of life is the hallmark merit over dialysis. Paired donation program should be encouraged in order to overcome shortage of kidney, which increases living donor pool. Outcome in living donor Kidney transplantation is always better than deceased donor transplantation. The prognosis of deceased donor transplantation (1 year Graft survival > 93% & 1 year patient survival > 90%) is also satisfactory with promising results. Therefore all the results were under acceptable standard limit. Thus, kidney transplantation (live or deceased donor) should be encouraged as primary modalities in the treatment of End Stage Renal Disease (ESRD). 展开更多
关键词 Kidney Transplantation (ktx) ESRD—End Stage Renal Disease Deceased Donor (DCD—Donor after Cardiac Death DBD—Donor after Brain Death)
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蝎毒素与小电导钙激活钾通道独特的相互作用(英文) 被引量:1
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作者 杨帆 陈宗运 吴英亮 《生理学报》 CAS CSCD 北大核心 2015年第3期255-260,共6页
分布在神经系统中的小电导Ca2+激活钾通道(small conductance Ca2+-activated potassium channels,SK通道)在学习、记忆及突触的可塑性过程中发挥了重要的作用。利用短链蝎毒素揭示了SK通道大部分药理学性质。不同于大部分电压门控钾通... 分布在神经系统中的小电导Ca2+激活钾通道(small conductance Ca2+-activated potassium channels,SK通道)在学习、记忆及突触的可塑性过程中发挥了重要的作用。利用短链蝎毒素揭示了SK通道大部分药理学性质。不同于大部分电压门控钾通道和大电导Ca2+激活钾通道,SK通道电流仅能被少量蝎毒素抑制。近来,研究揭示了SK通道选择性识别毒素的结构基础是SK通道细胞外孔区部位存在一种新颖的多肽筛选器。本综述总结了蝎毒素与SK通道独特的相互作用,这不仅对深入研究SK通道生理功能具有重要的作用,而且有助于促进SK通道相关疾病的药物开发。 展开更多
关键词 蛋白-蛋白相互作用 多肽筛选器 钾通道 α-ktx
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不同标签辅助的IBTX原核表达纯化及活性鉴定 被引量:1
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作者 赵志文 张峥 +2 位作者 刘浩文 黄智刚 吴颖 《现代生物医学进展》 CAS 2014年第7期1207-1211,共5页
目的:在原核体系中建立高效表达纯化IBTX(Iberiotoxin)的工艺,并比较不同标签对IBTX生物活性的影响。方法:利用引物搭桥的方法,经PCR扩增,获得IBTX编码基因,以此为模板分别构建了表达质粒PET32a(+)-IBTX、pGex-6p-1-IBTX、pDuet-MBP-IB... 目的:在原核体系中建立高效表达纯化IBTX(Iberiotoxin)的工艺,并比较不同标签对IBTX生物活性的影响。方法:利用引物搭桥的方法,经PCR扩增,获得IBTX编码基因,以此为模板分别构建了表达质粒PET32a(+)-IBTX、pGex-6p-1-IBTX、pDuet-MBP-IBTX,并将其转入E.coli(BL21)分别表达带有硫氧还原蛋白(TRX)、谷胱甘肽巯基转移酶(GST)、麦芽糖结合蛋白(MBP)标签的IBTX融合蛋白,在经过亲和层析、烟草蚀纹病毒(TEV)蛋白酶酶切、C18反向层析纯化后真空冻干得到IBTX干粉,以电生理实验检测其生物活性。结果:在三种标签帮助下通过原核体系表达出的IBTX都能够特异性的阻断大电导Ca2+激活的钾通道(BK)电流,其中MBP帮助折叠的m-IBTX活性最佳。结论:建立了IBTX在MBP标签帮助下的原核表达纯化工艺,为进一步研究蝎钾离子通道α家族神经毒素及其突变体的原核表达奠定了基础。 展开更多
关键词 钾离子通道毒素 IBTX 标签蛋白 BK
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