The crystal structure of (L-Arg)-B0 bovine insulin has been determined, using data to 0.21 nm and atomic parameters of 2Zn porcine insulin as a starting model, by the difference Fourier method, the restrained least sq...The crystal structure of (L-Arg)-B0 bovine insulin has been determined, using data to 0.21 nm and atomic parameters of 2Zn porcine insulin as a starting model, by the difference Fourier method, the restrained least square method and X-PLOR package, interspersed with careful review of the electron density, to a final R-factor of 0.182 and r.m.s. deviation of 0.002 2nm for the bond lengths and 4.3° for the bond angles. The electron densities of additional (L-Arg)-B0 residues to B-chain N-terminus of two monomers in each asymmetric unit are very dear. The crystallographic micro-environment of the N-terminus of the B-chain is different from that of rhombohedral 2-zinc insulin.展开更多
Using the effective Hamiltonian method, we analyze the B0-B0 mixing in the extension of the standard model （SM） where baryon number and lepton number are local gauge symmetries. The numerical results indicate the co...Using the effective Hamiltonian method, we analyze the B0-B0 mixing in the extension of the standard model （SM） where baryon number and lepton number are local gauge symmetries. The numerical results indicate the correction from the extra particles to the mass difference ArnB is significant. There is a 60% enhancement compared to the SM prediction for AraB at most, which agrees with the current experimental result.展开更多
基金Project supported by the Chinese Academy of Sciences and the National Natural Science Foundation of China.
文摘The crystal structure of (L-Arg)-B0 bovine insulin has been determined, using data to 0.21 nm and atomic parameters of 2Zn porcine insulin as a starting model, by the difference Fourier method, the restrained least square method and X-PLOR package, interspersed with careful review of the electron density, to a final R-factor of 0.182 and r.m.s. deviation of 0.002 2nm for the bond lengths and 4.3° for the bond angles. The electron densities of additional (L-Arg)-B0 residues to B-chain N-terminus of two monomers in each asymmetric unit are very dear. The crystallographic micro-environment of the N-terminus of the B-chain is different from that of rhombohedral 2-zinc insulin.
基金Supported by the National Natural Science Foundation of China(NNSFC)under Grant Nos.10975027,11275036,and 11047002
文摘Using the effective Hamiltonian method, we analyze the B0-B0 mixing in the extension of the standard model （SM） where baryon number and lepton number are local gauge symmetries. The numerical results indicate the correction from the extra particles to the mass difference ArnB is significant. There is a 60% enhancement compared to the SM prediction for AraB at most, which agrees with the current experimental result.
