Objective: To study the effects of total flavone of Abelmoschl Manihot L.Medic (TFA) on the function of platelets and to explore its mechanism. Methods: Rat models of artery-veins bypassing thrombus formation were use...Objective: To study the effects of total flavone of Abelmoschl Manihot L.Medic (TFA) on the function of platelets and to explore its mechanism. Methods: Rat models of artery-veins bypassing thrombus formation were used. The platelets of rabbits were collected. Platelet aggregation was induced by collagen and intracellular calcium ion concentration ([Ca 2+ ]i) was assayed by Fura-2 method. Results: TFA (25, 50, 100 mg/kg) significantly and dose-dependently reduced the weight of thrombus. TFA (0.025, 0.05, 0.1 mg/ml) possessed dose-dependant inhibitory effects on rabbits' platelet aggregation induced by collagen. TFA significantly reduced the resting and CaCl 2-induced increase of free intracellular calcium concentration ([Ca 2+ ]i) in rabbit platelet in vitro . Conclusion: TFA has an antiplatelet effect via the inhibition on the influx of Ca 2+ .展开更多
Flos Abelmoschus manihot has a long history of application.Recent studies show that total flavone of A.manihot(L.)Medic(TFA)has a wide range of pharmacological activity.This article is a review mainly on chemical comp...Flos Abelmoschus manihot has a long history of application.Recent studies show that total flavone of A.manihot(L.)Medic(TFA)has a wide range of pharmacological activity.This article is a review mainly on chemical composition,method of determination and pharmacologic action of TFA,and it raises research and development direction.展开更多
The study aimed to evaluate the endophytic fungi isolated from <em>Cocos nucifera</em> (L.) cotyledon for their possible antimicrobial activity. A total of twelve endophytic fungal species were successfull...The study aimed to evaluate the endophytic fungi isolated from <em>Cocos nucifera</em> (L.) cotyledon for their possible antimicrobial activity. A total of twelve endophytic fungal species were successfully isolated, from which five were selected and presumptively identified, including <em>Aspergillus niger</em>, <em>Raffaelea sp.</em>, <em>Aspergillus flavus</em>, <em>Cladosporium sp.</em> and <em>Pseudallescheria spp.</em> based on their macro and microscopic characters. The agar blocks of actively growing pure culture were assessed for antibacterial activity against five human pathogenic bacterial and fungal strains: <em>Escherichia coli</em> (BIOTECH 1634), <em>Bacillus subtilis</em> (BIOTECH 1697), <em>Staphylococcus aureus</em> (BIOTECH 1592), <em>Serratia marcescens</em> (BIOTECH 1748), and <em>Candida tropicalis</em> (BIOTECH 2085). Among the selected five endophytic used for antimicrobial activity screening, the <em>Raffaelea sp.</em> (CN-EF 2) has shown a high zone of inhibition of 11 mm against <em>Bacillus subtilis</em>, 18 mm against <em>Escherichia coli</em>, 15 mm against <em>Staphylococcus aureus</em>, and 11 mm against <em>Candida tropicalis</em>. Correspondingly, <em>Aspergillus niger</em> (CN-EF 1) has shown a zone of inhibition of 16 mm against <em>C. tropicalis</em>. The endophytic fungus <em>Raffaelea sp.</em> was effective against all test organisms except for <em>Serratia marcescens</em>, while <em>Aspergillus niger</em> was only effective for pathogenic fungi <em>C. albicans</em>. Hence, further in-depth studies of the isolated endophytic fungi are needed to determine the mechanisms behind the endophytes’ inhibitory effects.展开更多
基金Supported by the Natural Science Fund of Anhui Province (No 99044433) Supported by the Nature and Science of Education Office of Anhui Province (No 2003 KJ 224)
文摘Objective: To study the effects of total flavone of Abelmoschl Manihot L.Medic (TFA) on the function of platelets and to explore its mechanism. Methods: Rat models of artery-veins bypassing thrombus formation were used. The platelets of rabbits were collected. Platelet aggregation was induced by collagen and intracellular calcium ion concentration ([Ca 2+ ]i) was assayed by Fura-2 method. Results: TFA (25, 50, 100 mg/kg) significantly and dose-dependently reduced the weight of thrombus. TFA (0.025, 0.05, 0.1 mg/ml) possessed dose-dependant inhibitory effects on rabbits' platelet aggregation induced by collagen. TFA significantly reduced the resting and CaCl 2-induced increase of free intracellular calcium concentration ([Ca 2+ ]i) in rabbit platelet in vitro . Conclusion: TFA has an antiplatelet effect via the inhibition on the influx of Ca 2+ .
基金Jiangsu Natural Science Foundation Project(BK2008490).
文摘Flos Abelmoschus manihot has a long history of application.Recent studies show that total flavone of A.manihot(L.)Medic(TFA)has a wide range of pharmacological activity.This article is a review mainly on chemical composition,method of determination and pharmacologic action of TFA,and it raises research and development direction.
文摘The study aimed to evaluate the endophytic fungi isolated from <em>Cocos nucifera</em> (L.) cotyledon for their possible antimicrobial activity. A total of twelve endophytic fungal species were successfully isolated, from which five were selected and presumptively identified, including <em>Aspergillus niger</em>, <em>Raffaelea sp.</em>, <em>Aspergillus flavus</em>, <em>Cladosporium sp.</em> and <em>Pseudallescheria spp.</em> based on their macro and microscopic characters. The agar blocks of actively growing pure culture were assessed for antibacterial activity against five human pathogenic bacterial and fungal strains: <em>Escherichia coli</em> (BIOTECH 1634), <em>Bacillus subtilis</em> (BIOTECH 1697), <em>Staphylococcus aureus</em> (BIOTECH 1592), <em>Serratia marcescens</em> (BIOTECH 1748), and <em>Candida tropicalis</em> (BIOTECH 2085). Among the selected five endophytic used for antimicrobial activity screening, the <em>Raffaelea sp.</em> (CN-EF 2) has shown a high zone of inhibition of 11 mm against <em>Bacillus subtilis</em>, 18 mm against <em>Escherichia coli</em>, 15 mm against <em>Staphylococcus aureus</em>, and 11 mm against <em>Candida tropicalis</em>. Correspondingly, <em>Aspergillus niger</em> (CN-EF 1) has shown a zone of inhibition of 16 mm against <em>C. tropicalis</em>. The endophytic fungus <em>Raffaelea sp.</em> was effective against all test organisms except for <em>Serratia marcescens</em>, while <em>Aspergillus niger</em> was only effective for pathogenic fungi <em>C. albicans</em>. Hence, further in-depth studies of the isolated endophytic fungi are needed to determine the mechanisms behind the endophytes’ inhibitory effects.
基金The National Natural Science Foundation of China under Grant No.61502163(国家自然科学基金)the Research Program of the Education Department of Hunan Province under Grant No.2015C0589(湖南省教育厅科研项目)the Fundamental Research Funds for the Central Universities of Centr
