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胞内劳森菌LsaA蛋白的原核表达及间接ELISA抗体检测方法的建立 被引量:7
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作者 吴艳阳 杨东东 +4 位作者 高冬生 李永涛 常洪涛 王川庆 赵军 《畜牧兽医学报》 CAS CSCD 北大核心 2018年第4期786-793,共8页
为建立胞内劳森菌特异性抗体检测方法,将密码子优化合成的胞内劳森菌表面蛋白LsaA的编码基因克隆于pET32a(+)载体,在大肠杆菌BL21(DE3)中诱导表达。用纯化的重组LsaA蛋白作为包被抗原,通过一系列条件优化,建立了检测胞内劳森菌抗体的间... 为建立胞内劳森菌特异性抗体检测方法,将密码子优化合成的胞内劳森菌表面蛋白LsaA的编码基因克隆于pET32a(+)载体,在大肠杆菌BL21(DE3)中诱导表达。用纯化的重组LsaA蛋白作为包被抗原,通过一系列条件优化,建立了检测胞内劳森菌抗体的间接ELISA方法。结果显示:该方法可以特异地检测抗胞内劳森菌抗体,与大肠杆菌、猪霍乱沙门菌、猪流行性腹泻病毒和猪传染性胃肠炎病毒等常见猪腹泻病原的抗血清均无交叉反应。来自PCR检测粪便呈胞内劳森菌阳性猪的血清,用所建立ELISA检测均为胞内劳森菌抗体阳性。该方法具有较好的敏感性和重复性,阳性血清经过1∶800稀释检测结果仍为阳性,批内和批间重复试验的变异系数分别为0.352%~2.752%和0.877%~3.000%。应用建立的ELISA方法对河南省部分地区猪场胞内劳森菌的感染情况进行了血清流行病学调查,结果显示被检地区猪场均存在不同程度的胞内劳森菌抗体阳性,阳性率在13.3%~57.1%,平均阳性率为30.6%。结果表明,本研究所建立的ELISA方法可以用于胞内劳森菌抗体的检测,为胞内劳森菌感染的监测和流行病学调查提供了方法。 展开更多
关键词 猪增生性肠炎 胞内劳森菌 lsaa蛋白 间接ELISA
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Radio Propagation and Wireless Coverage of LSAA-Based 5G Millimeter-Wave Mobile Communication Systems 被引量:12
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作者 Haiming Wang Peize Zhang +1 位作者 Jing Li Xiaohu You 《China Communications》 SCIE CSCD 2019年第5期1-18,共18页
Millimeter-wave(mm Wave) communications will be used in fifth-generation(5G) mobile communication systems, but they experience severe path loss and have high sensitivity to physical objects, leading to smaller cell ra... Millimeter-wave(mm Wave) communications will be used in fifth-generation(5G) mobile communication systems, but they experience severe path loss and have high sensitivity to physical objects, leading to smaller cell radii and complicated network architectures. A coverage extension scheme using large-scale antenna arrays(LSAAs) has been suggested and theoretically proven to be cost-efficient in combination with ultradense small cell networks. To analyze and optimize the LSAA-based network deployments, a comprehensive survey of recent advances in statistical mmWave channel modeling is first presented in terms of channel parameter estimation, large-scale path loss models, and small-scale cluster models. Next, the measurement and modeling results at two 5G candidate mmWave bands(e.g., 28 GHz and 39 GHz) are reviewed and compared in several outdoor scenarios of interest, where the propagation characteristics make crucial contributions to wireless network designs. Finally, the coverage behaviors of systems employing a large number of antenna arrays are discussed, as well as some implications on future mmWave cellular network designs. 展开更多
关键词 FIFTH generation (5G) channel modeling large-scale antenna array(lsaa) MILLIMETER wave(mmWave) COMMUNICATIONS RADIO propagation measurements wireless COVERAGE
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Synthesis and application of lignin-based copolymer LSAA on controlling non-point source pollution resulted from surface runoff 被引量:8
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作者 LIU Chen WU Guangxia MU Huanzhen 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2008年第7期820-826,共7页
In this article,alkali lignin separated from paper pulp waste was grafted into a novel copolymer LSAA (a copolymer of lignin,starch, acrylamide,and acrylic acid).Its practical application effect and environmental safe... In this article,alkali lignin separated from paper pulp waste was grafted into a novel copolymer LSAA (a copolymer of lignin,starch, acrylamide,and acrylic acid).Its practical application effect and environmental safety were studied.The results of field simulation experiment indicated that the application of LSAA significantly affected the output of the runoff and pollutants.The runoff quantity was decreased by 16.67%-47.00%and the loads of total suspended solids (TSS),chemical oxygen demand (COD),total nit... 展开更多
关键词 application COPOLYMER a copolymer of lignin STARCH acrylamide and acrylic acid lsaa non-point source pollution (NPS) surface runoff
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Situs Inversus Totalis with Left-Sided Appendicitis: A Case Report
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作者 Mohsen Kamel Arid 《Open Journal of Clinical Diagnostics》 2020年第4期104-114,共11页
<b>Introduction:</b> Left-sided acute appendicitis (LSAA) develops in association with two types of congenital anomalies: situs inversus totalis (SIT) and midgut malrotation (MM). A Left sided appendicitis... <b>Introduction:</b> Left-sided acute appendicitis (LSAA) develops in association with two types of congenital anomalies: situs inversus totalis (SIT) and midgut malrotation (MM). A Left sided appendicitis is an ambiguous and difficult diagnosis to make. <b>Aim: </b>To present a proven case of left-sided acute appendicitis (LSAA) associated with situs inversus totalis (SIT). <b>Case</b> <b>Report:</b> A case of Left appendicitis was evaluated in a 28-year-old Asian male, who presented to our hospital in Feb. 2016, with lower abdominal pain more on left side and suspected diverticulitis or acute appendicitis with unusual appendix location. The patient doesn’t recall any history of abdominal surgery or about situs inversus totalis, abdominal and pelvic ultrasound was done, left iliac fossa appendicitis was diagnosed, Erect chest X-ray including upper abdomen revealed dextrocardia and stomach air on right side (situs inversus totalis), the patient underwent diagnostic Laproscop and Endoscopic resection of the appendix, with no incidents, and then discharged without complications, follow visits went unremarkable. <b>Conclusion: </b>The diagnosis of left lower quadrant pain is based on well-established clinical symptoms, physical examination and physician’s experience. 展开更多
关键词 Left Lower Quadrant Pain Left-Sided Appendicitis (lsaa) Midgut Malrotation (MM) Situs Inversus Totalis (SIT)
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