This paper aimed to study the change in MDA content and antioxidant enzyme activity of roots in wintering period under single sowing of alfalfa and mixed sowing of alfalfa and Bromus inermis Leyss.By the single sowing...This paper aimed to study the change in MDA content and antioxidant enzyme activity of roots in wintering period under single sowing of alfalfa and mixed sowing of alfalfa and Bromus inermis Leyss.By the single sowing of 4 alfalfa varieties with different cold resistance and mixed sowing of alfalfa varieties and Bromus inermis Leyss,the changes in MDA content,SOD,POD and CAT activity of alfalfa roots throughout the wintering period were measured.Results indicated that after single sowing of alfalfa and mixed sowing of alfalfa and Bromus inermis Leyss,the MDA content of roots showed an up-down-up trend with temperature; the CAT activity increased with the decreasing temperature but decreased when the temperature rose in the spring of the following year; the SOD and POD activity showed an increasing trend with sharp decline in temperature,and decreased when the temperature continued to decline,but increased with alfalfa reviving in the following year.The enzymic activity of the same variety under mixed sowing was higher than under single sowing.The membership function was used for comprehensive evaluation of cold resistance,and the cold resistance under different treatments was in the order of Wega7F + Bromus inermis Leyss >Wega7F > Xunlu + Bromus inermis Leyss > Xunlu > Aohan + Bromus inermis Leyss > Aohan > WL319HQ + Bromus inermis Leyss > WL319HQ.This was of great significance to the study on cold resistant alfalfa breeding and cultivation in cold areas of northern China.展开更多
以野生灵芝[Ganoderma lucidum(Leyss. ex Fr.)Karst.](D)、自然单核灵芝(Mo)、双核灵芝(Mu)的菌丝体为材料,利用高通量测序技术筛选差异表达基因,并进行GO分析和KEGG富集分析。通过差异基因分析,筛选出568个差异表达基因。GO分析结果表...以野生灵芝[Ganoderma lucidum(Leyss. ex Fr.)Karst.](D)、自然单核灵芝(Mo)、双核灵芝(Mu)的菌丝体为材料,利用高通量测序技术筛选差异表达基因,并进行GO分析和KEGG富集分析。通过差异基因分析,筛选出568个差异表达基因。GO分析结果表明,野生灵芝(D)与自然单核灵芝(Mo)菌丝体的差异表达基因主要富集在转录调控及DNA模板化、tRNA氨酰化、组蛋白甲基化等过程;野生灵芝(D)与双核灵芝(Mu)菌丝体的差异表达基因主要富集在染色质沉默、L-丝氨酸代谢、硫化合物代谢等过程;自然单核灵芝(Mo)与双核灵芝(Mu)菌丝体的差异表达基因主要富集在染色质沉默、DNA模板转录、谷氨酸分解等过程。KEGG富集结果表明,自然单核灵芝(Mo)与双核灵芝(Mu)菌丝体在代谢通路上有明显的差异,差异基因主要被注释到过氧化物酶、维生素代谢等通路。野生灵芝(D)与自然单核灵芝(Mo)菌丝体、野生灵芝(D)与双核灵芝(Mu)菌丝体的差异基因并没有KEGG富集的结果,说明其代谢通路没有明显差异。筛选出3个品系中表达量差异均显著的基因,共12个,并对基因的主要功能进行预测。12个基因中有5个基因可以匹配到GO库。GO注释结果表明,这5个基因主要被注释到氧化还原酶活性、金属离子结合、膜的调控与膜组成等方面。展开更多
基金Supported by Special Postdoctoral Funding in Heilongjiang Province(LBHTZ06020)
文摘This paper aimed to study the change in MDA content and antioxidant enzyme activity of roots in wintering period under single sowing of alfalfa and mixed sowing of alfalfa and Bromus inermis Leyss.By the single sowing of 4 alfalfa varieties with different cold resistance and mixed sowing of alfalfa varieties and Bromus inermis Leyss,the changes in MDA content,SOD,POD and CAT activity of alfalfa roots throughout the wintering period were measured.Results indicated that after single sowing of alfalfa and mixed sowing of alfalfa and Bromus inermis Leyss,the MDA content of roots showed an up-down-up trend with temperature; the CAT activity increased with the decreasing temperature but decreased when the temperature rose in the spring of the following year; the SOD and POD activity showed an increasing trend with sharp decline in temperature,and decreased when the temperature continued to decline,but increased with alfalfa reviving in the following year.The enzymic activity of the same variety under mixed sowing was higher than under single sowing.The membership function was used for comprehensive evaluation of cold resistance,and the cold resistance under different treatments was in the order of Wega7F + Bromus inermis Leyss >Wega7F > Xunlu + Bromus inermis Leyss > Xunlu > Aohan + Bromus inermis Leyss > Aohan > WL319HQ + Bromus inermis Leyss > WL319HQ.This was of great significance to the study on cold resistant alfalfa breeding and cultivation in cold areas of northern China.
文摘以野生灵芝[Ganoderma lucidum(Leyss. ex Fr.)Karst.](D)、自然单核灵芝(Mo)、双核灵芝(Mu)的菌丝体为材料,利用高通量测序技术筛选差异表达基因,并进行GO分析和KEGG富集分析。通过差异基因分析,筛选出568个差异表达基因。GO分析结果表明,野生灵芝(D)与自然单核灵芝(Mo)菌丝体的差异表达基因主要富集在转录调控及DNA模板化、tRNA氨酰化、组蛋白甲基化等过程;野生灵芝(D)与双核灵芝(Mu)菌丝体的差异表达基因主要富集在染色质沉默、L-丝氨酸代谢、硫化合物代谢等过程;自然单核灵芝(Mo)与双核灵芝(Mu)菌丝体的差异表达基因主要富集在染色质沉默、DNA模板转录、谷氨酸分解等过程。KEGG富集结果表明,自然单核灵芝(Mo)与双核灵芝(Mu)菌丝体在代谢通路上有明显的差异,差异基因主要被注释到过氧化物酶、维生素代谢等通路。野生灵芝(D)与自然单核灵芝(Mo)菌丝体、野生灵芝(D)与双核灵芝(Mu)菌丝体的差异基因并没有KEGG富集的结果,说明其代谢通路没有明显差异。筛选出3个品系中表达量差异均显著的基因,共12个,并对基因的主要功能进行预测。12个基因中有5个基因可以匹配到GO库。GO注释结果表明,这5个基因主要被注释到氧化还原酶活性、金属离子结合、膜的调控与膜组成等方面。
