In this study, 32 Luffa germplasm resources were collected from various regions in Zhejiang Province as experimental materials, to investigate 22 agronomic traits including fruit bearing habit, leaf margin, fruit ribb...In this study, 32 Luffa germplasm resources were collected from various regions in Zhejiang Province as experimental materials, to investigate 22 agronomic traits including fruit bearing habit, leaf margin, fruit ribbing and percentage of nodes with female flowers to total node. Based on the obtained experimental data, principal component analysis and cluster analysis were carried out using DPS software. The results showed that 22 agronomic traits could be integrated into 5 principal components, with the cumulative contributive percentage of 81. 308%. According to the correlations between the first five principal components and traits, 14 traits with great influences were screened. On the basis of principal component analysis, cluster analysis of 32 Luffa germplasm resources was conducted, which divided Luffa cylindrica and Luffa acutangula into two categories and six subcategories by Euclidean genetic distances. This study provided scientific basis for the collection, preservation, identification, creation and utilization of Luffa germplasm and parent selection in cross breeding of Luffa.展开更多
Luffa aegyptiaca fruit has been assayed for the presence of lignin peroxidase activity using veratryl alcohol as the substrate. The fruit juice contained activity of 3.14 U/ml which was much higher than 0.075 U/ml rep...Luffa aegyptiaca fruit has been assayed for the presence of lignin peroxidase activity using veratryl alcohol as the substrate. The fruit juice contained activity of 3.14 U/ml which was much higher than 0.075 U/ml reported in the culture filtrate of Phanarochaete chrysosporium ATCC-24725. The K<sub>m</sub> value of the lignin peroxidase using veratryl alcohol as the variable substrate in 50mM phosphate buffer pH 2.5 at 25°C was found to be 50 μM respectively. The pH and temperature optima of the lignin peroxidase were 2.4 and 22°C, respectively. The present article reports viable method to explore rich sources of lignin peroxidase from plants which can be used as a mediator in oxidative organic transformations within green chemistry domain ensuring ecofriendly synthesis of bioorganic molecules of pharmaceutical value.展开更多
Luffaculin 1 purified from the seeds of Luffa acutangula belongs to the type I ribosome-inactivating proteins （RIPs）. It has been crystallized by the vapor-diffusion method using polyethylene glycol as a precipitant...Luffaculin 1 purified from the seeds of Luffa acutangula belongs to the type I ribosome-inactivating proteins （RIPs）. It has been crystallized by the vapor-diffusion method using polyethylene glycol as a precipitant. The crystal is of space group P1 with a = 39.135, b = 46.813, c = 83.571 A, α = 891068,β = 80.009 and y = 72.143°, and has two molecules per asymmetric unit. X-ray data have been collected to be 1.4 A using a synchrotron source.展开更多
The effect of chemical modification, reinforcement structure and fiber weight ratio on the flexural proprieties of Luffa-polyester composites was studied. A unsaturated polyester matrix reinforced with a mat of Luffa ...The effect of chemical modification, reinforcement structure and fiber weight ratio on the flexural proprieties of Luffa-polyester composites was studied. A unsaturated polyester matrix reinforced with a mat of Luffa external wall fibers (ComLEMat), a short Luffa external wall fibers(ComLEBC) and a short Luffa core fi-bers (ComLCBC) was fabricated under various conditions of fibers treatments (combined process, acetylat-ing and cyanoethylating) and fiber weight ratio. It resorts that acetylating and cyanoethylating enhance the flexural strength and the flexural modulus. The fiber weight ratio influenced the flexural properties of com-posites. Indeed, a maximum value of strength and strain is observed over a 10% fiber weight ratio. The uses of various reinforcement structures were investigated. The enhancement of elongation at break and the strain values of the composite reinforced by natural mat was proved.展开更多
基金Supported by"San Nong Liu Fang"Science and Technology Cooperation Project of Zhejiang Province(ZNJF[2011]No.85)Major Project of Science and Technology of Zhejiang Province(2009C2006-1-8)
文摘In this study, 32 Luffa germplasm resources were collected from various regions in Zhejiang Province as experimental materials, to investigate 22 agronomic traits including fruit bearing habit, leaf margin, fruit ribbing and percentage of nodes with female flowers to total node. Based on the obtained experimental data, principal component analysis and cluster analysis were carried out using DPS software. The results showed that 22 agronomic traits could be integrated into 5 principal components, with the cumulative contributive percentage of 81. 308%. According to the correlations between the first five principal components and traits, 14 traits with great influences were screened. On the basis of principal component analysis, cluster analysis of 32 Luffa germplasm resources was conducted, which divided Luffa cylindrica and Luffa acutangula into two categories and six subcategories by Euclidean genetic distances. This study provided scientific basis for the collection, preservation, identification, creation and utilization of Luffa germplasm and parent selection in cross breeding of Luffa.
文摘Luffa aegyptiaca fruit has been assayed for the presence of lignin peroxidase activity using veratryl alcohol as the substrate. The fruit juice contained activity of 3.14 U/ml which was much higher than 0.075 U/ml reported in the culture filtrate of Phanarochaete chrysosporium ATCC-24725. The K<sub>m</sub> value of the lignin peroxidase using veratryl alcohol as the variable substrate in 50mM phosphate buffer pH 2.5 at 25°C was found to be 50 μM respectively. The pH and temperature optima of the lignin peroxidase were 2.4 and 22°C, respectively. The present article reports viable method to explore rich sources of lignin peroxidase from plants which can be used as a mediator in oxidative organic transformations within green chemistry domain ensuring ecofriendly synthesis of bioorganic molecules of pharmaceutical value.
基金This work was supported by the National Natural Science Foundation of China (No. 39970872) and Natural Science Foundation of Fujian Province (C97052)
文摘Luffaculin 1 purified from the seeds of Luffa acutangula belongs to the type I ribosome-inactivating proteins （RIPs）. It has been crystallized by the vapor-diffusion method using polyethylene glycol as a precipitant. The crystal is of space group P1 with a = 39.135, b = 46.813, c = 83.571 A, α = 891068,β = 80.009 and y = 72.143°, and has two molecules per asymmetric unit. X-ray data have been collected to be 1.4 A using a synchrotron source.
文摘The effect of chemical modification, reinforcement structure and fiber weight ratio on the flexural proprieties of Luffa-polyester composites was studied. A unsaturated polyester matrix reinforced with a mat of Luffa external wall fibers (ComLEMat), a short Luffa external wall fibers(ComLEBC) and a short Luffa core fi-bers (ComLCBC) was fabricated under various conditions of fibers treatments (combined process, acetylat-ing and cyanoethylating) and fiber weight ratio. It resorts that acetylating and cyanoethylating enhance the flexural strength and the flexural modulus. The fiber weight ratio influenced the flexural properties of com-posites. Indeed, a maximum value of strength and strain is observed over a 10% fiber weight ratio. The uses of various reinforcement structures were investigated. The enhancement of elongation at break and the strain values of the composite reinforced by natural mat was proved.
