The full-length genomic DNA of MCAT (Malonyl-CoA:acyl carrier protein transacylase) in Brassica napus was cloned. BnMCAT shares very high identity with AtMCAT in gene sequence and gene structure. A multiple alignment ...The full-length genomic DNA of MCAT (Malonyl-CoA:acyl carrier protein transacylase) in Brassica napus was cloned. BnMCAT shares very high identity with AtMCAT in gene sequence and gene structure. A multiple alignment of the protein sequence showed that BnMCAT shares high identity with other MCATs from E. coli and plants. BnMCAT was expressed in all tissues, such as roots, stems, leaves, flowers, and seeds, and no significant differences in the expression level were found in different embryo stages after pollination. According to an in vitro relative activity analysis, purified recombinant BnMCAT expressed in E. coli had transacylase activity. Although the relative activities of BnMCAT in crude extracts isolated from different staged embryos were similar and showed little variation, a higher relative activity was found in a crude extract isolated from embryos in comparison to leaves. Different relative activities of BnMCAT in crude extracts isolated from cultivars with different oil content were also found, suggesting that the activity of BnMCAT might be a decisive factor for a high oil content. Together, these results showed that BnMCAT is an important enzyme in the FAS system and indicate that BnMCAT might be a new target enzyme for future crop improvement through genetic engineering.展开更多
Identifying source information after river chemical spill occurrences is critical for emergency responses.However,the inverse uncertainty characteristics of this kind of pollution source inversion problem have not yet...Identifying source information after river chemical spill occurrences is critical for emergency responses.However,the inverse uncertainty characteristics of this kind of pollution source inversion problem have not yet been clearly elucidated.To fill this gap,stochastic analysis approaches,including a regional sensitivity analysis method,identifiability plot and perturbation methods,were employed to conduct an empirical investigation on generic inverse uncertainty characteristics under a well-accepted uncertainty analysis framework.Case studies based on field tracer experiments and synthetic numerical tracer experiments revealed several new rules.For example,the release load can be most easily inverted,and the source location is responsible for the largest uncertainty among the source parameters.The diffusion and convection processes are more sensitive than the dilution and pollutant attenuation processes to the optimization of objective functions in terms of structural uncertainty.The differences among the different objective functions are smaller for instantaneous release than for continuous release cases.Small monitoring errors affect the inversion results only slightly,which can be ignored in practice.Interestingly,the estimated values of the release location and time negatively deviate from the real values,and the extent is positively correlated with the relative size of the mixing zone to the objective river reach.These new findings improve decision making in emergency responses to sudden water pollution and guide the monitoring network design.展开更多
文摘The full-length genomic DNA of MCAT (Malonyl-CoA:acyl carrier protein transacylase) in Brassica napus was cloned. BnMCAT shares very high identity with AtMCAT in gene sequence and gene structure. A multiple alignment of the protein sequence showed that BnMCAT shares high identity with other MCATs from E. coli and plants. BnMCAT was expressed in all tissues, such as roots, stems, leaves, flowers, and seeds, and no significant differences in the expression level were found in different embryo stages after pollination. According to an in vitro relative activity analysis, purified recombinant BnMCAT expressed in E. coli had transacylase activity. Although the relative activities of BnMCAT in crude extracts isolated from different staged embryos were similar and showed little variation, a higher relative activity was found in a crude extract isolated from embryos in comparison to leaves. Different relative activities of BnMCAT in crude extracts isolated from cultivars with different oil content were also found, suggesting that the activity of BnMCAT might be a decisive factor for a high oil content. Together, these results showed that BnMCAT is an important enzyme in the FAS system and indicate that BnMCAT might be a new target enzyme for future crop improvement through genetic engineering.
基金funded by the China Postdoctoral Science Foundation(Grant No.2014M551249)the National Natural Science Foundation of China(Grant No.51509061)support was provided by the Southern University of Science and Technology(Grant No.G01296001).
文摘Identifying source information after river chemical spill occurrences is critical for emergency responses.However,the inverse uncertainty characteristics of this kind of pollution source inversion problem have not yet been clearly elucidated.To fill this gap,stochastic analysis approaches,including a regional sensitivity analysis method,identifiability plot and perturbation methods,were employed to conduct an empirical investigation on generic inverse uncertainty characteristics under a well-accepted uncertainty analysis framework.Case studies based on field tracer experiments and synthetic numerical tracer experiments revealed several new rules.For example,the release load can be most easily inverted,and the source location is responsible for the largest uncertainty among the source parameters.The diffusion and convection processes are more sensitive than the dilution and pollutant attenuation processes to the optimization of objective functions in terms of structural uncertainty.The differences among the different objective functions are smaller for instantaneous release than for continuous release cases.Small monitoring errors affect the inversion results only slightly,which can be ignored in practice.Interestingly,the estimated values of the release location and time negatively deviate from the real values,and the extent is positively correlated with the relative size of the mixing zone to the objective river reach.These new findings improve decision making in emergency responses to sudden water pollution and guide the monitoring network design.
