目的探讨脾虚证大鼠 IMC-胃肠运动-Mot 含量间的关系,为脾虚证本质提供客观依据.方法用传感器和双极银丝电极同步、动态地观察7 d 和14 d 脾虚证(pixu)大鼠胃和十二指肠消化间期综合肌电(IMC)电活动的及其机械运动,以及用放射免疫分析法...目的探讨脾虚证大鼠 IMC-胃肠运动-Mot 含量间的关系,为脾虚证本质提供客观依据.方法用传感器和双极银丝电极同步、动态地观察7 d 和14 d 脾虚证(pixu)大鼠胃和十二指肠消化间期综合肌电(IMC)电活动的及其机械运动,以及用放射免疫分析法(RIA)分析下丘脑、胃窦、十二指肠组织及血浆中的胃动素(Mot)的含量.结果与对照组比较,脾虚早期十二指肠Ⅰ相时程缩短(1.9±0.7 vs 4.2±0.3 min),Ⅱ相时程延长(6.5±0.4 vs 4.3±0.7min),脾虚晚期十二指肠Ⅱ相时程缩短(1.1±0.3 min).脾虚早期十二指肠收缩振幅(103.5±9.7 vs 78.9±6.3 mV/min)和收缩频率(21.4±1.9 vs 17.3±0.9次/min)均明显高于正常大鼠,而脾虚晚期表现为十二指肠收缩振幅(106.9±10.3mV/min)明显高于正常大鼠,收缩频率(11.7±1.2次/min)则明显减低.无论脾虚早期或晚期胃 IMC Ⅰ相时程延长(8.2±0.9和6.2±1.1 vs 3.5±0.8 min,运动功能均减弱(9.2±1.8和5.2±0.2 vs 13.4±1.3次/min,75.2±10.1和42.4±9.1vs 110.2±9.8mV/min).脾虚早期下丘脑、胃窦、十二指肠、空肠组织及血浆中 Mot 的含量升高(76.4±8.0,37.2±7.3.51.2±1.9,63.2±2.2和99.8±6.5 vs 53.2±9.3,29.5±7.6,35.6±3.0,46.6±7.0和82.7±9.3 ng/L),而脾虚晚期却下降(32.5±5.5,21.6±6.2,27.9±1.7,32.3±5.1和64.5±5.4 ng/L,P<0.05～P<0.01).结论脾虚程度不同,其各部位的 Mot 含量,十二指肠 IMC 的Ⅰ,Ⅱ相持续时间及机械运动不同;脾虚时 IMC 十二指肠运动-Mot 含量间存在正相关关系(r=0.9937),而 IMC 一胃运动-Mot 含量间为负相关关系(r=-0.9986).此体现了脾虚证在发展过程中由轻到重、由初期到久病的不同阶段,为认识脾虚证提供了定性定量客观依据.展开更多
Magneto-optical traps (MOTs) composed of magnetic fields and light fields have been widely utilized to cool andconfine microscopic particles. Practical technology applications require miniaturized MOTs. The advancemen...Magneto-optical traps (MOTs) composed of magnetic fields and light fields have been widely utilized to cool andconfine microscopic particles. Practical technology applications require miniaturized MOTs. The advancement of planaroptics has promoted the development of compact MOTs. In this article, we review the development of compact MOTs basedon planar optics. First, we introduce the standardMOTs. We then introduce the gratingMOTs with micron structures, whichhave been used to build cold atomic clocks, cold atomic interferometers, and ultra-cold sources. Further, we introducethe integrated MOTs based on nano-scale metasurfaces. These new compact MOTs greatly reduce volume and powerconsumption, and provide new opportunities for fundamental research and practical applications.展开更多
Mitochondrial organelle transplantation (MOT) is an innovative strategy for the treatment of mitochondrial dysfunction such as cardiac ischemic reperfusion injuries, Parkinson’s diseases, brain and spinal cord injuri...Mitochondrial organelle transplantation (MOT) is an innovative strategy for the treatment of mitochondrial dysfunction such as cardiac ischemic reperfusion injuries, Parkinson’s diseases, brain and spinal cord injuries, and amyotrophic lateral sclerosis (ALS). However, one of the major challenges for widespread usage is a methodology for preservation of isolated mitochondria. Extracellular vesicles (EVs) are phospholipid bilayer-enclosed vesicles released from cells. EVs carry a cargo of proteins, nucleic acids, lipids, metabolites, and even organelles such as mitochondria. Purpose: To test if EVs enhance the stability of isolated mitochondria. Methods: We mixed isolated mitochondria of fibroblasts with EVs of mesenchymal stromal cells (imEVs) (9:1 in volume) and stored the mixture at 2°C - 6°C for different time periods. We measured morphology, mitochondrial membrane potential (MMP) and mitochondrial ATP content at 0, 2, 5 days. Key findings: After 2 days of storage, the mito-chondria without imEVs lost approximate 70% MMP (RFU: 1822 ± 68), compared to the fresh mitochondria (RFU: 5458 ± 52) (p 0.05). In agreement with MMP, mitochondria without imEVs lost significant mitochondrial ATP content (p 0.05), after 2 days of cold storage, compared to fresh mitochondria. Microscopy showed that imEVs promoted aggregation of isolated mitochondria. Summary: The preliminary data showed that imEVs enhanced the stability of isolated mitochondria in cold storage.展开更多
文摘目的探讨脾虚证大鼠 IMC-胃肠运动-Mot 含量间的关系,为脾虚证本质提供客观依据.方法用传感器和双极银丝电极同步、动态地观察7 d 和14 d 脾虚证(pixu)大鼠胃和十二指肠消化间期综合肌电(IMC)电活动的及其机械运动,以及用放射免疫分析法(RIA)分析下丘脑、胃窦、十二指肠组织及血浆中的胃动素(Mot)的含量.结果与对照组比较,脾虚早期十二指肠Ⅰ相时程缩短(1.9±0.7 vs 4.2±0.3 min),Ⅱ相时程延长(6.5±0.4 vs 4.3±0.7min),脾虚晚期十二指肠Ⅱ相时程缩短(1.1±0.3 min).脾虚早期十二指肠收缩振幅(103.5±9.7 vs 78.9±6.3 mV/min)和收缩频率(21.4±1.9 vs 17.3±0.9次/min)均明显高于正常大鼠,而脾虚晚期表现为十二指肠收缩振幅(106.9±10.3mV/min)明显高于正常大鼠,收缩频率(11.7±1.2次/min)则明显减低.无论脾虚早期或晚期胃 IMC Ⅰ相时程延长(8.2±0.9和6.2±1.1 vs 3.5±0.8 min,运动功能均减弱(9.2±1.8和5.2±0.2 vs 13.4±1.3次/min,75.2±10.1和42.4±9.1vs 110.2±9.8mV/min).脾虚早期下丘脑、胃窦、十二指肠、空肠组织及血浆中 Mot 的含量升高(76.4±8.0,37.2±7.3.51.2±1.9,63.2±2.2和99.8±6.5 vs 53.2±9.3,29.5±7.6,35.6±3.0,46.6±7.0和82.7±9.3 ng/L),而脾虚晚期却下降(32.5±5.5,21.6±6.2,27.9±1.7,32.3±5.1和64.5±5.4 ng/L,P<0.05～P<0.01).结论脾虚程度不同,其各部位的 Mot 含量,十二指肠 IMC 的Ⅰ,Ⅱ相持续时间及机械运动不同;脾虚时 IMC 十二指肠运动-Mot 含量间存在正相关关系(r=0.9937),而 IMC 一胃运动-Mot 含量间为负相关关系(r=-0.9986).此体现了脾虚证在发展过程中由轻到重、由初期到久病的不同阶段,为认识脾虚证提供了定性定量客观依据.
基金the National Key Research and Development Program of China(Grant No.2022YFA1404104)the National Natural Science Foundation of China(Grant Nos.12025509 and 12104521)Fundamental Research Project of Shenzhen(Grant No.JCYJ20230808105009018).
文摘Magneto-optical traps (MOTs) composed of magnetic fields and light fields have been widely utilized to cool andconfine microscopic particles. Practical technology applications require miniaturized MOTs. The advancement of planaroptics has promoted the development of compact MOTs. In this article, we review the development of compact MOTs basedon planar optics. First, we introduce the standardMOTs. We then introduce the gratingMOTs with micron structures, whichhave been used to build cold atomic clocks, cold atomic interferometers, and ultra-cold sources. Further, we introducethe integrated MOTs based on nano-scale metasurfaces. These new compact MOTs greatly reduce volume and powerconsumption, and provide new opportunities for fundamental research and practical applications.
文摘Mitochondrial organelle transplantation (MOT) is an innovative strategy for the treatment of mitochondrial dysfunction such as cardiac ischemic reperfusion injuries, Parkinson’s diseases, brain and spinal cord injuries, and amyotrophic lateral sclerosis (ALS). However, one of the major challenges for widespread usage is a methodology for preservation of isolated mitochondria. Extracellular vesicles (EVs) are phospholipid bilayer-enclosed vesicles released from cells. EVs carry a cargo of proteins, nucleic acids, lipids, metabolites, and even organelles such as mitochondria. Purpose: To test if EVs enhance the stability of isolated mitochondria. Methods: We mixed isolated mitochondria of fibroblasts with EVs of mesenchymal stromal cells (imEVs) (9:1 in volume) and stored the mixture at 2°C - 6°C for different time periods. We measured morphology, mitochondrial membrane potential (MMP) and mitochondrial ATP content at 0, 2, 5 days. Key findings: After 2 days of storage, the mito-chondria without imEVs lost approximate 70% MMP (RFU: 1822 ± 68), compared to the fresh mitochondria (RFU: 5458 ± 52) (p 0.05). In agreement with MMP, mitochondria without imEVs lost significant mitochondrial ATP content (p 0.05), after 2 days of cold storage, compared to fresh mitochondria. Microscopy showed that imEVs promoted aggregation of isolated mitochondria. Summary: The preliminary data showed that imEVs enhanced the stability of isolated mitochondria in cold storage.
