Ambient-Condition Strategy for Production of Hollow Ga_(2)O_(3)@rGO Crystalline Nanostructures Toward Efficient Lithium Storage

Crystallineγ-Ga_(2)O_(3)@rGO core-shell nanostructures are synthesized in gram scale,which are accomplished by a facile sonochemical strategy under ambient condition.They are composed of uniformγ-Ga_(2)O_(3)nanospheres encapsulated by reduced graphene oxide(rGO)nanolayers,and their formation is mainly attributed to the existed opposite zeta potential between the Ga_(2)O_(3)and rGO.The as-constructed lithium-ion batteries(LIBs)based on as-fabricatedγ-Ga_(2)O_(3)@rGO nanostructures deliver an initial discharge capacity of 1000 mAh g^(-1)at 100 mA g^(-1)and reversible capacity of 600 mAh g^(-1)under 500 mA g^(-1)after 1000 cycles,respectively,which are remarkably higher than those of pristineγ-Ga_(2)O_(3)with a much reduced lifetime of 100 cycles and much lower capacity.Ex situ XRD and XPS analyses demonstrate that the reversible LIBs storage is dominant by a conversion reaction and alloying mechanism,where the discharged product of liquid metal Ga exhibits self-healing ability,thus preventing the destroy of electrodes.Additionally,the rGO shell could act robustly as conductive network of the electrode for significantly improved conductivity,endowing the efficient Li storage behaviors.This work might provide some insight on mass production of advanced electrode materials under mild condition for energy storage and conversion applications.

Conditioned medium from human dental pulp stem cells treats spinal cord injury by inhibiting microglial pyroptosis

Human dental pulp stem cell transplantation has been shown to be an effective therapeutic strategy for spinal cord injury.However,whether the human dental pulp stem cell secretome can contribute to functional recovery after spinal cord injury remains unclear.In the present study,we established a rat model of spinal cord injury based on impact injury from a dropped weight and then intraperitoneally injected the rats with conditioned medium from human dental pulp stem cells.We found that the conditioned medium effectively promoted the recovery of sensory and motor functions in rats with spinal cord injury,decreased expression of the microglial pyroptosis markers NLRP3,GSDMD,caspase-1,and interleukin-1β,promoted axonal and myelin regeneration,and inhibited the formation of glial scars.In addition,in a lipopolysaccharide-induced BV2 microglia model,conditioned medium from human dental pulp stem cells protected cells from pyroptosis by inhibiting the NLRP3/caspase-1/interleukin-1βpathway.These results indicate that conditioned medium from human dental pulp stem cells can reduce microglial pyroptosis by inhibiting the NLRP3/caspase-1/interleukin-1βpathway,thereby promoting the recovery of neurological function after spinal cord injury.Therefore,conditioned medium from human dental pulp stem cells may become an alternative therapy for spinal cord injury.

Enhancing fly ash utilization in backfill materials treated with CO_(2)carbonation under ambient conditions

The environmental concerns resulting from coal-fired power generation that produces large amounts of CO_(2)and fly ash are of great interest.To mitigate,this study aims to develop a novel carbonated CO_(2)-fly ash-based backfill(CFBF)material under ambient conditions.The performance of CFBF was investigated for different fly ash-cement ratios and compared with non-CO_(2)reacted samples.The fresh CFBF slurry conformed to the Herschel-Bulkley model with shear thinning characteristics.After carbonation,the yield stress of the fresh slurry increased significantly by lowering fly ash ratio due to gel formation.The setting times were accelerated,resulting in approximately 40.6%of increased early strength.The final strength decreased when incorporating a lower fly ash ratio(50%and 60%),which was related to the existing heterogeneous pores caused by rapid fluid loss.The strength increased with fly ash content above 70%because additional C-S(A)-H and silica gels were characterized to precipitate on the grain surface,so the binding between particles increased.The C-S(A)-H gel was developed through the pozzolanic reaction,where CaCO_(3)was the prerequisite calcium source obtained in the CO_(2)-fly ash reaction.Furthermore,the maximum CO_(2)uptake efficiency was 1.39 mg-CO_(2)/g-CFBF.The CFBF material is feasible to co-dispose CO_(2)and fly ash in the mine goaf as negative carbon backfill materials,and simultaneously mitigates the strata movement and water lost in post-subsurface mining.

A Novel Computationally Efficient Approach to Identify Visually Interpretable Medical Conditions from 2D Skeletal Data

Timely identification and treatment of medical conditions could facilitate faster recovery and better health.Existing systems address this issue using custom-built sensors,which are invasive and difficult to generalize.A low-complexity scalable process is proposed to detect and identify medical conditions from 2D skeletal movements on video feed data.Minimal set of features relevant to distinguish medical conditions:AMF,PVF and GDF are derived from skeletal data on sampled frames across the entire action.The AMF(angular motion features)are derived to capture the angular motion of limbs during a specific action.The relative position of joints is represented by PVF(positional variation features).GDF(global displacement features)identifies the direction of overall skeletal movement.The discriminative capability of these features is illustrated by their variance across time for different actions.The classification of medical conditions is approached in two stages.In the first stage,a low-complexity binary LSTM classifier is trained to distinguish visual medical conditions from general human actions.As part of stage 2,a multi-class LSTM classifier is trained to identify the exact medical condition from a given set of visually interpretable medical conditions.The proposed features are extracted from the 2D skeletal data of NTU RGB+D and then used to train the binary and multi-class LSTM classifiers.The binary and multi-class classifiers observed average F1 scores of 77%and 73%,respectively,while the overall system produced an average F1 score of 69%and a weighted average F1 score of 80%.The multi-class classifier is found to utilize 10 to 100 times fewer parameters than existing 2D CNN-based models while producing similar levels of accuracy.

Study on Identification and Fermentation Condition of Strain ZY-19-2 with Inhibitory Effect against Tobacco Black Shank (Phytophtora parasitica var. nicotianae Tucker)

[Objective] The paper was to identify stain ZY-19-2 with inhibitory effect against tobacco black shank （Phytophtora parasitica var.nicotianae Tucker）, and study the fermentation condition of the strain. [Method]A strain ZY-19-2 with strong inhibitory effect against P. parasitica were isolated and screened from tobacco rhizosphere soil samples, and identified according to its morphological characteristics. The chitinase production activity of the strain under different culture conditions was also studied. [Result] For stain ZY-19-2 Paecilomyces lilacinus, the optimal fermentation conditions were as follows： 1.2% colloidal chitin as carbon source, 1% peptone as nitrogen source, 0.1% Tween 80 as surfactant, initial pH of fermentation broth at 6.0, the fermentation time of 60 h, inoculum amount at 1%, shaker speed at 120 r/min. The highest enzyme activity reached 0.216 U/ml. [Conclusion]The optimization of fermentation condition of strain ZY-19-2 lay foundation for large-scale production of cheap and efficient chitinase and chitin oligosaccharides, as well as application of the strain for control of tobacco black shank.

HBP、NT-proBNP及β_(2)-MG与脓毒血症病情严重程度的相关性及预后评估价值

目的探讨肝素结合蛋白(HBP)、氨基末端脑钠肽前体(NT-proBNP)及β_(2)-微球蛋白(β_(2)-MG)与脓毒血症病情严重程度的相关性,并分析其对患者预后的评估价值。方法选取2021年9月至2023年6月在上海市第六人民医院金山分院诊治的脓毒血症患者69例作为研究对象,对其临床资料进行回顾性分析。按病情严重程度将患者分为一般脓毒血症组(23例)、严重脓毒血症组(23例)和脓毒血症休克组(23例),另选取同期在上海市第六人民医院金山分院住院的非脓毒血症患者23例为对照组。根据28 d病死率将脓毒血症患者分为生存组和死亡组。检测并比较各组患者血清HBP、NT-proBNP、β_(2)-MG水平及急性生理学与慢性健康状况评分系统Ⅱ(APACHEⅡ)评分,采用Pearson相关分析血清HBP、NT-proBNP、β_(2)-MG水平与APACHEⅡ评分的相关性,并利用受试者工作特征(ROC)曲线分析其对脓毒血症患者死亡的预测价值。结果一般脓毒血症组、严重脓毒血症组、脓毒血症休克组血清HBP、NT-proBNP、β_(2)-MG水平及APACHEⅡ评分均高于对照组(P<0.05);HBP、NT-proBNP、β_(2)-MG水平及APACHEⅡ评分表现为一般脓毒血症组<严重脓毒血症组<脓毒血症休克组,任意两组间比较,差异均有统计学意义(P<0.05)。死亡组血清HBP、NT-proBNP、β_(2)-MG水平及APACHEⅡ评分均高于生存组(P<0.05)。Pearson相关分析结果显示,脓毒血症患者血清HBP、NT-proBNP、β_(2)-MG水平与APACHEⅡ评分呈正相关(r=0.645、0.776、0.593,P<0.05)。ROC曲线分析结果显示,HBP、NT-proBNP、β_(2)-MG联合检测预测脓毒血症患者死亡的AUC为0.870(95%CI:0.730~1.000),明显高于HBP、NT-proBNP、β_(2)-MG单独检测的AUC[0.768(95%CI:0.598~0.937)、0.792(95%CI:0.653~0.932)、0.814(95%CI:0.645~0.984)],差异均有统计学意义(Z=2.705,P=0.007;Z=2.496,P=0.013;Z=2.126,P=0.033)。结论检测血清HBP、NT-proBNP、β_(2)-MG水平有助于判断脓毒血症患者病情严重程度及评估患者预后,3项指标联合检测可预测患者死亡风险。

基于广泛靶向代谢组学解析不同树龄及栽培条件的野酿2号腺枝毛葡萄类黄酮差异

【目的】基于广泛靶向代谢组学分析不同树龄及栽培条件的野酿2号腺枝毛葡萄类黄酮差异代谢物情况,为提升葡萄及葡萄酒品质提供理论参考依据。【方法】以不同树龄及栽培条件(地域和架式)的野酿2号腺枝毛葡萄为试材,测定粒重、pH、可溶性固形物、可滴定酸含量及亮度值(L^(*))、红—绿色度值(a^(*))、黄—蓝色度值(b^(*)),并利用超高效液相色谱—串联质谱仪对其成熟期葡萄果实进行广泛靶向代谢组学分析。【结果】不同树龄及栽培条件的野酿2号腺枝毛葡萄理化指标之间存在不同程度的差异,栽培地域对葡萄果实理化指标影响较大,栽种于相思湖葡萄示范基地的果实成熟度更好,但果粒较小、果皮颜色较浅;栽培架式及树龄对葡萄果实的粒重影响较小,对糖酸含量影响较大;同一地域采用篱架栽培的葡萄果实较棚架栽培成熟度更好,且树龄越大,果实成熟度越好。从不同树龄及栽培条件的成熟期葡萄果实中共鉴定出11类789种代谢物,其中黄酮类最多,为187种。12个样品中共存在271个差异代谢物,共聚为7个类群,黄酮类化合物在各亚类中占比最高,特别是第Ⅱ类群,占比高达59%,其次是第Ⅰ类群,占比为49%。利用差异代谢物能明确区分来自不同地域、架式和树龄的葡萄果实样品,其中地域样品间的差异最大,且黄酮类化合物是造成差异的最主要化合物。明阳双季葡萄示范基地栽培较相思湖葡萄示范基地栽培更利于杨梅素、槲皮素、表儿茶素及甲基花翠素等类黄酮化合物的积累,5年树龄较8年树龄更有利于促进山柰酚、儿茶素和花青素的积累,而棚架栽培较篱架栽培更利于黄烷-3-醇、花青素双糖苷和花葵素双糖苷的积累。可溶性固形物含量与黄酮醇中的槲皮素和杨梅素含量呈显著负相关,L^(*)和a^(*)均与杨梅素呈显著负相关。【结论】不同树龄及栽培条件的野酿2号腺枝毛葡萄果实类黄酮代谢物含量存在差异,其中地域影响最明显。

高糖培养条件下lncRNA CCAT2调控心肌细胞损伤和凋亡的机制研究

目的 探讨长链非编码RNA(lncRNA)结肠癌相关转录本2(CCAT2)在高糖培养条件下心肌细胞中的表达变化及通过Wnt/β-catenin信号通路调控心肌细胞损伤和凋亡的机制。方法 体外培养人心肌细胞AC16,将细胞分为4组,对照组、高糖组、高糖+干扰RNA阴性对照组、高糖+干扰RNA组(干扰RNA的靶标为lncRNA CCAT2)。采用CCK8法及流式细胞术检测细胞增殖和凋亡率;实时荧光定量PCR(qPCR)检测lncRNA CCAT2、β-catenin、转录因子7类似物2(TCF7L2)、Caspase-3、c-Myc、细胞周期蛋白(Cyclin)D1基因的表达;Western blot检测β-catenin、TCF7L2、CyclinD1蛋白表达;检测细胞培养液中超氧化物歧化酶(SOD)、乳酸脱氢酶(LDH)和天门冬氨酸氨基转移酶(AST)活性。结果 高糖培养条件下的AC16心肌细胞lncRNA CCAT2的基因表达显著高于对照组(P<0.05),同时β-catenin、TCF7L2、Caspase-3、c-Myc、CyclinD1基因表达显著上调(P<0.05),细胞增殖受到抑制(P<0.05),凋亡率增加(P<0.05),细胞培养液中LDH和AST活性显著升高(P<0.05),细胞中SOD活性显著降低(P<0.05)。高糖培养条件下,RNA干扰lncRNA CCAT2表达后,细胞增殖增加(P<0.05),细胞凋亡率显著下降(P<0.05),β-catenin、TCF7L2、Caspase-3、c-Myc、CyclinD1基因的表达下调(P<0.05),β-catenin(细胞核)、TCF7L2、CyclinD1蛋白表达显著下调(P<0.05),β-catenin(细胞质)蛋白表达显著上调(P<0.05)。结论 高糖条件下,lncRNA CCAT2可通过Wnt/β-catenin信号通路调控心肌细胞损伤和凋亡。

致密砂岩气藏稳产后期动用效果评价与动用潜力分析:以子洲气田山2气藏为例

以子洲气田山2气藏为例,利用钻井及生产动态资料,通过对地质储量、动储量、累产气、累产水和动静储量比的计算与分析,采用综合评判指数法和类比法,详细评价了山2致密砂岩气藏动用效果和动用潜力。结果表明:山2气藏平面动用严重不均衡。7个井区中,主砂体带中部的榆30井区动用效果最好,属Ⅰ类动用区;主砂体带南部的洲3和北部榆81井区动用效果中等,为Ⅱ类动用区;主砂体周边的榆69、榆48、麒麟沟和榆29井区动用效果差,属Ⅲ类动用区。初步预测山2气藏整体动用潜力为198.1×10^(8) m^(3)。其中,Ⅲ类区的动用潜力为174.7×10^(8) m^(3),占88.2%,是山2气藏后续挖潜重点。

回热器对CO_(2)驻车空调系统制热性能影响的研究

为了提升驻车空调的制热性能,搭建了一套带回热器的跨临界CO_(2)驻车空调系统,在焓差室中实验研究了有无回热器时气冷器出口温度与排气压力对系统的性能影响。结果表明:在排气压力分别为8.5、9.0、9.5 MPa时,气冷器出口温度从20℃升高至35℃时,制热COP_(h)降低幅度分别为29.7%、19.9%、16.4%;系统使用回热器后最优排气压力下降,下降幅度分别为4.4%、6.1%,对应的最大COP_(h)增加幅度分别为3.2%、3.7%。通过曲面拟合得到了系统使用回热器时提升制热性能的排气压力与气冷器出口温度关联式,该式对回热器在跨临界CO_(2)驻车空调系统制热模式下的实际应用有一定指导意义。

蝉花中N^(6)-(2-羟乙基)-腺苷提取工艺优化及结构鉴定

在单因素实验基础上,采用响应面法考察液料比、水浴温度、水浴时间对提取物中N6-(2-羟乙基)-腺苷[N^(6)-(2-hydroxyethyl) adenosine,HEA]含量的影响,优化出HEA的最佳提取工艺:以超纯水为溶剂、液料比118∶1、超声时间25 min、水浴温度24℃、水浴时间3.8 h,获得提取物中HEA含量为(0.836±0.030) mg·g^(-1)。采用大孔树脂、酸性氧化铝层析柱和半制备高效液相色谱仪(semi preparative high performance liquid chromatography,SP-HPLC)分离纯化提取液中HEA,采用核磁共振及电喷雾电离质谱(electrospray ionization mass spectrometry,ESI-MS)和核磁共振氢谱(proton nuclear magnetic resonance spectroscopy,^(1)H NMR)鉴定分离出的物质为HEA,经高效液相色谱(high performance liquid chromatography,HPLC)分析其纯度为99.32%。

UNIQUE COMMON FIXED POINT OF A FAMILY OF SELF-MAPS WITH SAME TYPE CONTRACTIVE CONDITION IN 2-METRIC SPACE

In this paper, we prove that a family of self-maps {Ti,j}i,j∈N in 2-metric space has a unique common fixed point if （i） {Ti,j}i,j∈N satisfies the same type contractive condition for each j ∈ N; （ii） Tm,μ .Tn,v = Tn,v.Tm.μ for all m,n,μ,v ∈ N with μ≠v. Our main result generalizes and improves many known unique common fixed point theorems in 2-metric spaces.

Uniqueness of Common Fixed Points for a Family of Mappings with <i>Φ</i>-Contractive Condition in 2-Metric Spaces

In this paper, we will introduce a class of 5-dimensional functions Φ and prove that a family of self-mappings {Ti,j} iεN in 2-metric space have an unique common fixed point if 1) {Ti,j} iεN satisfies Φj-contractive condition, where ΦjεΦ, for each jεN;2) Tm,μ n,v for all m,n,μ,vεN with μ ≠ v . Our main result generalizes and unifies many known unique common fixed point theorems in 2-metric spaces.

Local structural evolutions of CuO/ZnO/Al2O3 catalyst for methanol synthesis under operando conditions studied by in situ quick X-ray absorption spectroscopy

In situ quick X-ray absorption spectroscopy(QXAFS) at the Cu and Zn K-edge under operando conditions has been used to unravel the Cu/Zn interaction and identify possible active site of CuO/ZnO/Al_2O_3 catalyst for methanol synthesis. In this work, the catalyst, whose activity increases with the reaction temperature and pressure, was studied at calcined, reduced, and reacted conditions. TEM and EDX images for the calcined and reduced catalysts showed that copper was distributed uniformly at both conditions. TPR profile revealed two reduction peaks at 165 and 195 °C for copper species in the calcined catalyst. QXAFS results demonstrated that the calcined form consisted mainly of a mixed Cu O and Zn O, and it was progressively transformed into Cu metal particles and dispersed Zn O species as the reduction treatment. It was demonstrated that activation of the catalyst precursor occurred via a Cu^+intermediate, and the active catalyst predominantly consisted of metallic Cu and Zn O evenunder higher pressures. Structure of the active catalyst did not change with the temperature or pressure, indicating that the role of the Zn was mainly to improve Cu dispersion.This indicates the potential of QXAFS method in studying the structure evolutions of catalysts in methanol synthesis.

Influence of extrusion conditions on microstructure and mechanical properties of Mg-2Gd-0.3Zr magnesium alloy

In general,different extrusion conditions will affect the microstructure of magnesium alloys and further determine the mechanical properties.The effects of extrusion parameters and heat treatment processes such as extrusion speed,pre-forging,annealing time,extrusion ratio and cooling rate on the microstructure,texture evolution and tensile properties of Mg-2Gd-0.3Zr alloys were investigated in this study.Compared with the as-cast alloy,the extrusion process significantly refines the grains and exhibit the rare earth texture.With the increase of extrusion speed and annealing time,the growth of recrystallized grains is accelerated,leading to the increase of elongation.Large pre-forging deformation achieves to refine the grains by promoting recrystallization nucleation,resulting in increased strength of Mg-2Gd-0.3Zr alloy.Decreasing the extrusion ratio or increasing the cooling rate will introduce coarse un-DRXed grains,which transformed the texture into the basal texture.In particular,the effect of rapid cooling on refining the recrystallized grains is also obvious.Different extrusion conditions influence the mechanical properties of the Mg-2Gd-0.3Zr alloy through the grain size,proportion of non-recrystallization region and texture type.

聚乙烯亚胺和Mg2+荧光增敏快速检测四环素类抗生素

四环素类抗生素(TCs)是一类广谱抗菌药物,被广泛应用于治疗人畜疾病且效果显著,但过度使用TCs造成其在动物性食品中的残留问题会对自然环境和人体健康造成严重的危害。因此,对食品环境中TCs的残留进行快速检测是十分必要的。迄今为止,对TCs在酸性溶液中荧光检测相关的研究报道较少。在弱酸性条件下,TCs属于弱荧光物质,但与富有氨基的阳离子表面活性剂聚乙烯亚胺(PEI)结合时,在370 nm的激发下,在525 nm处产生较强的绿色荧光;当TCs与PEI和二价金属镁离子(Mg^(2+))共存时,TCs的荧光强度被进一步增强。本研究利用PEI和Mg^(2+)在弱酸性条件下对TCs的协同荧光增敏效应,构建了一种快速、简单、高灵敏检测残留TCs的荧光检测法。实验结果表明:在最优检测条件下,该体系对TCs的定量线性检测范围为5 nmol/L~2μmol/L,其中,美他环素(MTC)、四环素(TC)、土霉素(OTC)的相关系数R^(2)分别为0.939,0.997和0.999,检出限(LOD)分别为3.6,2.7和4.8 nmol/L,均远低于中国和欧盟的限量标准。本方法对MTC、TC、OTC表现出很高的特异性,与常见的其他抗生素均不产生干扰。在实际的牛奶检测中,该体系对TCs的加标回收率为91.64%~104.05%,相对标准偏差为1.20%~10.62%。本方法可适用于酸性体系中残留TCs的分析,为水体和食品中残留TCs检测提供了一种新的依据和思路。

Porous LaFeO3 nanofiber with oxygen vacancies as an efficient electrocatalyst for N2 conversion to NH3 under ambient conditions

Electrocatalytic N2 reduction to NH3 under ambient conditions is an eco-friendly and sustainable alternative to the traditional Haber-Bosch process. However, inhibited by the high activation barrier of N2, this process needs efficient electrocatalysts to adsorb and activate the N2, enabling the N2 reduction reaction(NRR). Herein, we report that porous LaFeO3 nanofiber with oxygen vacancies acts as an efficient NRR electrocatalyst with abundant active sites to enhance the adsorption and activation of N2. When tested in 0.1 M HCl, such electrocatalyst achieves a high Faradaic efficiency of 8.77% and a large NH3 yield rate of 18.59 μg h–1 mgcat–1.at-0.55 V versus reversible hydrogen electrode. This catalyst also shows high long-term electrochemical stability and excellent selectivity for NH3 formation. Density functional theory calculations reveal that, by introducing oxygen vacancy on LaFeO3, the subsurface metallic ions are exposed with newly localized electronic states near the Fermi level, which facilitates the adsorption and activation of N2 molecules as well as the subsequent hydrogenation reactions.

Stabilization of Discrete-time 2-D T-S Fuzzy Systems Based on New Relaxed Conditions

This paper is concerned with the problem of stabilization of the Roesser type discrete-time nonlinear 2-D system that plays an important role in many practical applications. First, a discrete-time 2-D T-S fuzzy model is proposed to represent the underlying nonlinear 2-D system. Second, new quadratic stabilization conditions are proposed by applying relaxed quadratic stabilization technique for 2-D case. Third, for sake of further reducing conservatism, new non-quadratic stabilization conditions are also proposed by applying a new parameter-dependent Lyapunov function, matrix transformation technique, and relaxed technique for the underlying discrete-time 2-D T-S fuzzy system. Finally, a numerical example is provided to illustrate the effectiveness of the proposed results.

Fine particle aggregating and flotation behavior induced by high intensity conditioning of a CO_2 saturation slurry

The influence on fine particle aggregation and flotation behavior induced by high intensity conditioning(HIC) from saturated of the slurry with CO2 saturation was investigated.Bubble size measurements were conducted.The effect of dissolved gas,xanthate addition and agitation speed on fine sphalerite particle aggregation-and flotation-behavior were studied.The results show that during HIC in air or CO2 saturated water xanthate acts as a frother.The dissolved gas content in the pulp and HIC play a synergistic role in promoting fine particle aggregation and hence flotation;a significantly enhanced aggregation of fine sphalerite particles in a CO2 saturated slurry by HIC is observed.The aggregate size increased when the agitation speed was increased from 700 r/min to 1500 r/min.Increasing the HIC speed to 1500 r/min caused a positive impact on flotation kinetics.Further increasing the speed to 2000 r/min resulted in an adverse effect on flotation kinetics.

Effect of glycine site/NMDA receptor antagonist MRZ2/576 on the conditioned place preference and locomotor activity induced by morphine in mice

Objective: To study the effect of glycine site/NMDA (N-methyl-D-aspartate) receptor antagonist MRZ2/576 on the conditioned place preference (CPP) and locomotor activity induced by morphine in mice. Methods: Different doses (1.25, 2.5 and 5 mg/kg, i.p.) of MRZ2/576 were used to evaluate the effect of MRZ2/576 on the acquisition and expression of CPP induced by morphine (5 mg/kg) in mice. In addition, we examined the locomotor activity of mice in conditioning and testing phase of CPP paradigm. Results: MRZ2/576 alone could not establish place preference, but a 5 mg/kg dose of MRZ2/576 could block both acquisition and expression of morphine-induced CPP. In testing phase of CPP, there was no statistical difference for locomotor activity between the groups; injection of MRZ2/576 showed a dose-dependent decrease of locomotor activity on both control and morphine-treated mice, especially 5 mg/kg of MRZ2/576 significantly suppressed the locomotor activity of mice. Conclusion: Based on the present results, we assume that MRZ2/576 can antagonize the rewarding effect of morphine, suggesting that this glycine site/NMDA receptor antagonist could be used to treat addictions due to its light side effect profile.