Polyhydroxyalkanoates(PHAs)are diversiform biopolyesters with a similar structure and different side chain groups,synthesized by a variety of microorganism.Due to their excellent biodegradability and biocompatibility,...Polyhydroxyalkanoates(PHAs)are diversiform biopolyesters with a similar structure and different side chain groups,synthesized by a variety of microorganism.Due to their excellent biodegradability and biocompatibility,PHAs have been used for many applications,including medical implants,antibacterial agents and bioengineering.Nano-architecture is an emerging area for the use of PHAs.This review summarizes the current status and challenges of PHAs-based particles on the micro-and nano-scale,including their production,degradation,biological safety,and surface functionalization.We also focus on the applications of PHA particles in drug delivery systems,environment protection,tissue engi-neering,vaccine engineering,food science,biotechnology and cosmetics.Finally,the future development trends of PHAs-based particles are prospected.展开更多
Bacterial strains isolated from an oil field at Dagang in Tianjin were found to produce novel polyesters termed polyhydroxyalkanoates (PHAs). Monomers with medium side chain length in the polymers produced by two bact...Bacterial strains isolated from an oil field at Dagang in Tianjin were found to produce novel polyesters termed polyhydroxyalkanoates (PHAs). Monomers with medium side chain length in the polymers produced by two bacterial strains No 0806 and No 1317 grown on glucose were observed using GC(gas chromatography), IR (infrared spectroscopy), and GC MS (gas chromatography mass spectroscopy) analysis. The structures of the PHAs reported in this study are different from the common polyhydroxybutyrate (PHB) often stored by bacteria.展开更多
Aeromonas hydrophila 4AK4 was grown on mixed substrates of soybean oil and lauric acid for the production of polyhydroxyalkanoate copolymer consisting of 3 hydroxybutyrate (3HB) and 3 hydroxyhexanoate (3HHx). A m...Aeromonas hydrophila 4AK4 was grown on mixed substrates of soybean oil and lauric acid for the production of polyhydroxyalkanoate copolymer consisting of 3 hydroxybutyrate (3HB) and 3 hydroxyhexanoate (3HHx). A maximal poly(3 hydroxybutyrate co 3 hydroxyhexanoate) (PHBHHx) content of 49.13% in dry cells was obtained in a shake flask culture. PHBHHx of 6.26 g/L was produced in a fermentation experiment over 48 h on a sole carbon source containing 100 g/L soybean oil, while 12.40 g/L PHBHHx was produced on a mixed carbon source containing 80 g/L soybean and 20 g/L lauric acid over the same period of time, resulting in a polyhydroxyalkanoate (PHA) productivity of 0.25 g/(L·h). The results show that mixed carbon sources are suitable for industrialized production of PHBHHx from A. hydrophila 4AK4, as the mixed carbon sources also overcome the foaming problem that occurs when lauric acid is employed as a sole carbon source in PHBHHx production.展开更多
基金supported by Grants from Key Science and Technology Plan Projects in Zigong (grant No.2022ZCNKY07)National Natural Science Foundation of China (grant No.31900950)Jiangxi Provincial Natural Science Foundation (grant No.20212BAB214048).
文摘Polyhydroxyalkanoates(PHAs)are diversiform biopolyesters with a similar structure and different side chain groups,synthesized by a variety of microorganism.Due to their excellent biodegradability and biocompatibility,PHAs have been used for many applications,including medical implants,antibacterial agents and bioengineering.Nano-architecture is an emerging area for the use of PHAs.This review summarizes the current status and challenges of PHAs-based particles on the micro-and nano-scale,including their production,degradation,biological safety,and surface functionalization.We also focus on the applications of PHA particles in drug delivery systems,environment protection,tissue engi-neering,vaccine engineering,food science,biotechnology and cosmetics.Finally,the future development trends of PHAs-based particles are prospected.
文摘Bacterial strains isolated from an oil field at Dagang in Tianjin were found to produce novel polyesters termed polyhydroxyalkanoates (PHAs). Monomers with medium side chain length in the polymers produced by two bacterial strains No 0806 and No 1317 grown on glucose were observed using GC(gas chromatography), IR (infrared spectroscopy), and GC MS (gas chromatography mass spectroscopy) analysis. The structures of the PHAs reported in this study are different from the common polyhydroxybutyrate (PHB) often stored by bacteria.
基金Supported by"985"Foundation of Tsinghua University
文摘Aeromonas hydrophila 4AK4 was grown on mixed substrates of soybean oil and lauric acid for the production of polyhydroxyalkanoate copolymer consisting of 3 hydroxybutyrate (3HB) and 3 hydroxyhexanoate (3HHx). A maximal poly(3 hydroxybutyrate co 3 hydroxyhexanoate) (PHBHHx) content of 49.13% in dry cells was obtained in a shake flask culture. PHBHHx of 6.26 g/L was produced in a fermentation experiment over 48 h on a sole carbon source containing 100 g/L soybean oil, while 12.40 g/L PHBHHx was produced on a mixed carbon source containing 80 g/L soybean and 20 g/L lauric acid over the same period of time, resulting in a polyhydroxyalkanoate (PHA) productivity of 0.25 g/(L·h). The results show that mixed carbon sources are suitable for industrialized production of PHBHHx from A. hydrophila 4AK4, as the mixed carbon sources also overcome the foaming problem that occurs when lauric acid is employed as a sole carbon source in PHBHHx production.
