Objective: To investigate the effect of Compound Danshen Dripping Pills (CDDP) on oxidative stress after ischemia/reperfusion (I/R) injury in the rat retina. Methods: Adult male SD rats were randomly divided into 3 gr...Objective: To investigate the effect of Compound Danshen Dripping Pills (CDDP) on oxidative stress after ischemia/reperfusion (I/R) injury in the rat retina. Methods: Adult male SD rats were randomly divided into 3 groups: sham (group A), I/R (group B), and I/R plus CDDP (group C). Retinal ischemia/reperfusion injury (RIRI) was introduced by increasing the intraocular pressure (IOP) to 110 mmHg for 60 min via cannulation into the anterior chamber. Right after the insult, CDDP was administered intragastrically (450 mg/kg/d) for 7 days. The levels of malondialdehyde (MDA), the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) in the retinal tissues were determined on d1 and d7 after the ischemic insult. Results: Following ischemia, the MDA levels in group B and group C were significantly higher than those in group A (p < 0.01). CDDP significantly lowered MDA levels in group C when compared with group B (p < 0.01). The activities of SOD, GSH-Px and CAT were higher in group A than in group B and group C (p < 0.01). CDDP could increase the activities of SOD, GSH-Px and CAT remarkably in group C when compared with group B (p < 0.01). Conclusion: CDDP can protect the retina from I/R injury through reducing oxidative stress, and thus may be a promising method for the treatment of ischemic retinal disorders.展开更多
肾脏缺血时,坏死和凋亡的肾小管上皮及血管内皮细胞会释放出高迁移率族蛋白(high mobility group box 1,HMGB1)。HMGB1可能是诱导肾脏缺血再灌注损伤(renal ischemia and reperfusion injury,RIRI)早期的内源性危险信号。本研究主要阐...肾脏缺血时,坏死和凋亡的肾小管上皮及血管内皮细胞会释放出高迁移率族蛋白(high mobility group box 1,HMGB1)。HMGB1可能是诱导肾脏缺血再灌注损伤(renal ischemia and reperfusion injury,RIRI)早期的内源性危险信号。本研究主要阐述了在RIRI早期胞外HMGB1的来源以及与RIRI的关系。HMGB1可促使天然调节型T细胞(nTreg)丧失炎症抑制作用甚至可能翻转为促炎性细胞,该分子同时诱导巨噬细胞分泌大量炎性因子,并作用于活化的巨噬细胞使其自分泌HMGB1,二者共同作用造成肾脏缺血再灌注早期炎症损伤。如在损伤早期阻断HMGB1的多重作用,不仅可以消除巨噬细胞的炎症反应,而且可能恢复nTreg的炎症抑制作用从而减轻炎症反应,可能会对肾脏起到更好的保护作用。展开更多
文摘Objective: To investigate the effect of Compound Danshen Dripping Pills (CDDP) on oxidative stress after ischemia/reperfusion (I/R) injury in the rat retina. Methods: Adult male SD rats were randomly divided into 3 groups: sham (group A), I/R (group B), and I/R plus CDDP (group C). Retinal ischemia/reperfusion injury (RIRI) was introduced by increasing the intraocular pressure (IOP) to 110 mmHg for 60 min via cannulation into the anterior chamber. Right after the insult, CDDP was administered intragastrically (450 mg/kg/d) for 7 days. The levels of malondialdehyde (MDA), the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) in the retinal tissues were determined on d1 and d7 after the ischemic insult. Results: Following ischemia, the MDA levels in group B and group C were significantly higher than those in group A (p < 0.01). CDDP significantly lowered MDA levels in group C when compared with group B (p < 0.01). The activities of SOD, GSH-Px and CAT were higher in group A than in group B and group C (p < 0.01). CDDP could increase the activities of SOD, GSH-Px and CAT remarkably in group C when compared with group B (p < 0.01). Conclusion: CDDP can protect the retina from I/R injury through reducing oxidative stress, and thus may be a promising method for the treatment of ischemic retinal disorders.
文摘肾脏缺血时,坏死和凋亡的肾小管上皮及血管内皮细胞会释放出高迁移率族蛋白(high mobility group box 1,HMGB1)。HMGB1可能是诱导肾脏缺血再灌注损伤(renal ischemia and reperfusion injury,RIRI)早期的内源性危险信号。本研究主要阐述了在RIRI早期胞外HMGB1的来源以及与RIRI的关系。HMGB1可促使天然调节型T细胞(nTreg)丧失炎症抑制作用甚至可能翻转为促炎性细胞,该分子同时诱导巨噬细胞分泌大量炎性因子,并作用于活化的巨噬细胞使其自分泌HMGB1,二者共同作用造成肾脏缺血再灌注早期炎症损伤。如在损伤早期阻断HMGB1的多重作用,不仅可以消除巨噬细胞的炎症反应,而且可能恢复nTreg的炎症抑制作用从而减轻炎症反应,可能会对肾脏起到更好的保护作用。
