目的检测生殖器形成抑制基因-1（SMG-1）在肾癌组织中的表达情况，研究SMG-1的表达与肾癌临床病理参数的关系及对预后的临床意义。方法应用免疫组织化学方法检测SMG-1在58例肾癌组织及32例癌旁组织的表达情况。用SPSS18.0软件包...目的检测生殖器形成抑制基因-1（SMG-1）在肾癌组织中的表达情况，研究SMG-1的表达与肾癌临床病理参数的关系及对预后的临床意义。方法应用免疫组织化学方法检测SMG-1在58例肾癌组织及32例癌旁组织的表达情况。用SPSS18.0软件包进行统计处理，采用χ2检验SMG-1表达与临床病理因素的关系，Kaplan-Meier法及Cox回归模型用于肾癌的生存分析。结果SMG-1在肾癌组织阳性表达低于癌旁组织（20/58 vs. 23/32,P=0.001）。按照TNM分期及Fuhrman分级，在Ⅰ＋Ⅱ期肾癌患者中SMG-1表达高于在Ⅲ＋Ⅳ期（17/39 vs. 3/19, P=0.036），在G1＋G2肾癌患者中SMG-1表达高于G3＋G4患者（18/41 vs. 2/17,P=0.01）。SMG-1高表达的患者总生存期长于SMG-1低表达的患者（P=0.018）。SMG-1高表达是肾癌预后良好的独立因素／[风险比（HR）=0.289, 95% CI=0.126~0.662,P=0.003]。结论SMG-1异常表达可能参与肾癌的发生和进展。SMG-1的高表达可作为预测肾癌良好预后的独立指标。展开更多
目的探讨组织生殖器形成抑制基因-1(suppressor with morphogenetic effect on genitalia-1,SMG-1)mRNA和SOX4mRNA检测在乳腺癌监测中的应用价值。方法回顾性分析2008年5月～2015年5月于咸阳市第一人民医院就诊的45例乳腺浸润性导管癌(...目的探讨组织生殖器形成抑制基因-1(suppressor with morphogenetic effect on genitalia-1,SMG-1)mRNA和SOX4mRNA检测在乳腺癌监测中的应用价值。方法回顾性分析2008年5月～2015年5月于咸阳市第一人民医院就诊的45例乳腺浸润性导管癌(IDC组)患者和32例乳腺非典型导管增生(ADH组)患者的临床资料,同时选择30例癌旁正常乳腺组织作为对照组,应用实时逆转录-聚合酶链反应(RT-PCR)技术检测组织SMG-1mRNA和SOX4mRNA的表达,比较分析两标志物的表达与乳腺癌的关系。结果组织SOX4mRNA在对照组、ADH组和IDC组中的异常率和表达水平分别为20.0%,53.1%,77.7%和0.21±0.07,0.57±0.13,0.89±0.18;组织SMG-1mRNA在三组中的异常率和表达水平分别为83.3%,60.0%,26.6%和0.92±0.21,0.62±0.12,0.25±0.09。与对照组比较,组织SOX4mRNA在ADH组与IDC组中的异常率和表达水平均显著增高,而SMG-1mRNA则显著降低;与ADH组比较,组织SOX4mRNA在IDC组中的异常率和表达水平显著增高,而SMG-1mRNA则显著降低;以上比较的差异均有统计学意义(F_(表达水平)=326.23～353.97,χ■=4.51～24.24,P<0.01或P<0.05)。血清CA153在对照组、ADH组和IDC组中的异常率和水平分别为3.3%,25.0%,44.4%和13.2±11.3,50.3±36.2,132.0±77.9μg/ml;血清CEA在三组中的异常率和表达水平分别为3.3%,12.5%,20.0%和2.3±1.6,19.3±7.6,47.3±31.9 ng/ml。与ADH组和对照组比较,血清CA153和CEA水平在IDC组中显著增高,其差异有统计学意义(χ~2=5.37～5.47,均P<0.01)。在45例乳腺癌患者中,组织SMG-1mRNA和SOX4mRNA表达具有负相关性(r=-0.832,P<0.01)。SMG-1mRNA表达与血清CA153和CEA水平有负相关性(r=-0.767,-0.623,P<0.01),而SOX4mRNA则有正相关性(r=0.779,0.605,P<0.01)。组织SOX4mRNA的表达与乳腺癌的肿瘤大小和淋巴结转移具有显著相关性(χ~2=5.37～5.47,均P<0.05)。SMG-1mRNA表达与乳腺癌的肿瘤大小、TNM分期、淋巴结转移及组织学分级均有相关性(均χ~2=4.60～6.03,P<0.05)。结论检测组织SMG-1mRNA和SOX4mRNA表达可应用于乳腺癌的早期发现,降低乳腺癌的漏检率。展开更多
Background:According to the World Health Organization,about 350 million people worldwide are suffering from depression.It's reported that depression has been linked to several circadian rhythm perturbations,sugges...Background:According to the World Health Organization,about 350 million people worldwide are suffering from depression.It's reported that depression has been linked to several circadian rhythm perturbations,suggesting a disruption of the circadian clock system in affective disorders.The present study investigates the possible molecular mechanism of Shimian granules(SMG)in treating depression via restoring disrupted circadian rhythms.Method:Firstly,network pharmacology approach was used to identify the compounds and potential targets of SMG in TCMIP and BATMAN-TCM database.Secondly,the differential expression genes were obtained by gene expression profiling in GEO database(GSE56931,GSE98793).Further,protein-protein interactions(PPI)network was used to screen out core targets by STRING v11.Moreover,functional enrichment was carried out in DAVID database.Conclusively,the"herbs-compounds-targets-pathways"network was established to explore the mechanism of SMG in the treatment of depression.Result:It was found out that 65 compounds,18 targets and three pathways contributed to SMG in treating depression by regulating disrupted circadian rhythms,which might relate to core targets TNF,IL10,VDR in cAMP and calcium signaling pathway.Conclusion:Network pharmacology combined with gene expression profiling exhibited a powerful means to investigate the possible mechanism of formula,which contributes to theoretical basis for further study of SMG in the treatment of depression.展开更多
文摘目的检测生殖器形成抑制基因-1（SMG-1）在肾癌组织中的表达情况，研究SMG-1的表达与肾癌临床病理参数的关系及对预后的临床意义。方法应用免疫组织化学方法检测SMG-1在58例肾癌组织及32例癌旁组织的表达情况。用SPSS18.0软件包进行统计处理，采用χ2检验SMG-1表达与临床病理因素的关系，Kaplan-Meier法及Cox回归模型用于肾癌的生存分析。结果SMG-1在肾癌组织阳性表达低于癌旁组织（20/58 vs. 23/32,P=0.001）。按照TNM分期及Fuhrman分级，在Ⅰ＋Ⅱ期肾癌患者中SMG-1表达高于在Ⅲ＋Ⅳ期（17/39 vs. 3/19, P=0.036），在G1＋G2肾癌患者中SMG-1表达高于G3＋G4患者（18/41 vs. 2/17,P=0.01）。SMG-1高表达的患者总生存期长于SMG-1低表达的患者（P=0.018）。SMG-1高表达是肾癌预后良好的独立因素／[风险比（HR）=0.289, 95% CI=0.126~0.662,P=0.003]。结论SMG-1异常表达可能参与肾癌的发生和进展。SMG-1的高表达可作为预测肾癌良好预后的独立指标。
文摘目的探讨组织生殖器形成抑制基因-1(suppressor with morphogenetic effect on genitalia-1,SMG-1)mRNA和SOX4mRNA检测在乳腺癌监测中的应用价值。方法回顾性分析2008年5月～2015年5月于咸阳市第一人民医院就诊的45例乳腺浸润性导管癌(IDC组)患者和32例乳腺非典型导管增生(ADH组)患者的临床资料,同时选择30例癌旁正常乳腺组织作为对照组,应用实时逆转录-聚合酶链反应(RT-PCR)技术检测组织SMG-1mRNA和SOX4mRNA的表达,比较分析两标志物的表达与乳腺癌的关系。结果组织SOX4mRNA在对照组、ADH组和IDC组中的异常率和表达水平分别为20.0%,53.1%,77.7%和0.21±0.07,0.57±0.13,0.89±0.18;组织SMG-1mRNA在三组中的异常率和表达水平分别为83.3%,60.0%,26.6%和0.92±0.21,0.62±0.12,0.25±0.09。与对照组比较,组织SOX4mRNA在ADH组与IDC组中的异常率和表达水平均显著增高,而SMG-1mRNA则显著降低;与ADH组比较,组织SOX4mRNA在IDC组中的异常率和表达水平显著增高,而SMG-1mRNA则显著降低;以上比较的差异均有统计学意义(F_(表达水平)=326.23～353.97,χ■=4.51～24.24,P<0.01或P<0.05)。血清CA153在对照组、ADH组和IDC组中的异常率和水平分别为3.3%,25.0%,44.4%和13.2±11.3,50.3±36.2,132.0±77.9μg/ml;血清CEA在三组中的异常率和表达水平分别为3.3%,12.5%,20.0%和2.3±1.6,19.3±7.6,47.3±31.9 ng/ml。与ADH组和对照组比较,血清CA153和CEA水平在IDC组中显著增高,其差异有统计学意义(χ~2=5.37～5.47,均P<0.01)。在45例乳腺癌患者中,组织SMG-1mRNA和SOX4mRNA表达具有负相关性(r=-0.832,P<0.01)。SMG-1mRNA表达与血清CA153和CEA水平有负相关性(r=-0.767,-0.623,P<0.01),而SOX4mRNA则有正相关性(r=0.779,0.605,P<0.01)。组织SOX4mRNA的表达与乳腺癌的肿瘤大小和淋巴结转移具有显著相关性(χ~2=5.37～5.47,均P<0.05)。SMG-1mRNA表达与乳腺癌的肿瘤大小、TNM分期、淋巴结转移及组织学分级均有相关性(均χ~2=4.60～6.03,P<0.05)。结论检测组织SMG-1mRNA和SOX4mRNA表达可应用于乳腺癌的早期发现,降低乳腺癌的漏检率。
基金This research has been financially supported by the Special Support Scheme for Shaanxi Province,and the Subject Innovation Team of Shaanxi University of Chinese Medicine(#2019-YS01)Shaanxi province administration of traditional Chinese medicine(#2021-ZZ-JC018).
文摘Background:According to the World Health Organization,about 350 million people worldwide are suffering from depression.It's reported that depression has been linked to several circadian rhythm perturbations,suggesting a disruption of the circadian clock system in affective disorders.The present study investigates the possible molecular mechanism of Shimian granules(SMG)in treating depression via restoring disrupted circadian rhythms.Method:Firstly,network pharmacology approach was used to identify the compounds and potential targets of SMG in TCMIP and BATMAN-TCM database.Secondly,the differential expression genes were obtained by gene expression profiling in GEO database(GSE56931,GSE98793).Further,protein-protein interactions(PPI)network was used to screen out core targets by STRING v11.Moreover,functional enrichment was carried out in DAVID database.Conclusively,the"herbs-compounds-targets-pathways"network was established to explore the mechanism of SMG in the treatment of depression.Result:It was found out that 65 compounds,18 targets and three pathways contributed to SMG in treating depression by regulating disrupted circadian rhythms,which might relate to core targets TNF,IL10,VDR in cAMP and calcium signaling pathway.Conclusion:Network pharmacology combined with gene expression profiling exhibited a powerful means to investigate the possible mechanism of formula,which contributes to theoretical basis for further study of SMG in the treatment of depression.
