The effects of AAV-TGF beta_1 and AAV-TGF beta_3 on promoting synthesis ofglycosaminoglycan and collagen type II of dedifferentiated rabbit lumbar disc NP cells were studiedin this work.The rabbit lumbar disc NP cells...The effects of AAV-TGF beta_1 and AAV-TGF beta_3 on promoting synthesis ofglycosaminoglycan and collagen type II of dedifferentiated rabbit lumbar disc NP cells were studiedin this work.The rabbit lumbar disc NP cells were isolated and cultured.The earlier and laterdedifferentiated NP cells were established by subculture.The AAV transfection efficiency todedifferentiated NP cells was analyzed with AAV-EGFP in vitro.After dedifferentiated NP cells weretransfected by AAV-TGFp,or AAV-TGF beta_3,their biological effects on promoting synthesis ofglycosaminoglycan or collagen type II were detected and compared by the methods of 35S incorporationor immunoblotting.The experimental results showed that AAV could transfect efficiently the earlierdedifferentiated NP cells,but its transfection rate was shown to be at a low level to the laterdedifferentiated NP cells.Both AAV-TGF beta_1,and AAV-TGF beta_3 could promote the earlierdedifferentiated NP cells to synthesize glycosaminoglycan and collagen type II,and the effect ofAAV-TGFp,was better than that of AAV-TGF beta_3.For the later dedifferentiated NP cells,the AAV-TGFbeta_3 could promote their synthesis,but AAV-TGFp,could slightly inhibit theirsynthesis.Therefore,AAV-TGFp,and AAV-TGF beta_3 could be used for the earlier dedifferentiated NPcells,and the TGF beta_3 could be used as the objective gene for the later dedifferentiated NPcells.展开更多
基金Supported by the National Natural Science Foundation of China (Grant No. 30271318)
文摘The effects of AAV-TGF beta_1 and AAV-TGF beta_3 on promoting synthesis ofglycosaminoglycan and collagen type II of dedifferentiated rabbit lumbar disc NP cells were studiedin this work.The rabbit lumbar disc NP cells were isolated and cultured.The earlier and laterdedifferentiated NP cells were established by subculture.The AAV transfection efficiency todedifferentiated NP cells was analyzed with AAV-EGFP in vitro.After dedifferentiated NP cells weretransfected by AAV-TGFp,or AAV-TGF beta_3,their biological effects on promoting synthesis ofglycosaminoglycan or collagen type II were detected and compared by the methods of 35S incorporationor immunoblotting.The experimental results showed that AAV could transfect efficiently the earlierdedifferentiated NP cells,but its transfection rate was shown to be at a low level to the laterdedifferentiated NP cells.Both AAV-TGF beta_1,and AAV-TGF beta_3 could promote the earlierdedifferentiated NP cells to synthesize glycosaminoglycan and collagen type II,and the effect ofAAV-TGFp,was better than that of AAV-TGF beta_3.For the later dedifferentiated NP cells,the AAV-TGFbeta_3 could promote their synthesis,but AAV-TGFp,could slightly inhibit theirsynthesis.Therefore,AAV-TGFp,and AAV-TGF beta_3 could be used for the earlier dedifferentiated NPcells,and the TGF beta_3 could be used as the objective gene for the later dedifferentiated NPcells.
