The interaction between calf thymus DNA （ctDNA） and Pd （Ⅱ） meso-tetralds- （4-N-methyl pyridiniumyl） porphyrin （Pd-TMPyP） was investigated by steady solid-substrate room temperature phosphorescence （SS-RTP）....The interaction between calf thymus DNA （ctDNA） and Pd （Ⅱ） meso-tetralds- （4-N-methyl pyridiniumyl） porphyrin （Pd-TMPyP） was investigated by steady solid-substrate room temperature phosphorescence （SS-RTP）. The SS-RTP intensity and lifetime of Pd-TMPyP enhanced with the increasing ctDNA. The anion quenching experiment of Pd-TMPyP indicated that Pd-TMPyP intercalated into ctDNA, which was also approved by UV-Vis spectra. Keywords： Pd-TMPyP, ctDNA, SS-RTP, intercalation展开更多
The effect of various metal ions on the DNA mediated energy transfer between simultaneously bound drugs was investigated using spectroscopic methods.It was found that addition of divalent metal ions(Mg^(2+),Ca^(2+),Mn...The effect of various metal ions on the DNA mediated energy transfer between simultaneously bound drugs was investigated using spectroscopic methods.It was found that addition of divalent metal ions(Mg^(2+),Ca^(2+),Mn^(2+),Co^(2+)and Ni^(2+))resulted in further decrease of the ethidium fluorescence intensity,while a small increase was observed in the TMPyP emission band,implying that the energy of excited ethidium was transferred to TMPyP.This DNA-mediated quenching efficiency between ethidium and TMPyP was significantly enhanced by the presence of all metal ions.Among the divalent metal ions,alkali earth metal ions and Mn^(2+)displayed higher quenching efficiencies than other transition metal ions.The distances required to permit the energy transfer between the two drugs in DNA were calculated as 68,66,62,48and 38in the presence of100μmol·L^(-1 )of Mg^(2+),Ca^(2+),Mn^(2+),Co^(2+)and Ni ^(2+)ion,respectively.The disturbed binding conformation of TMPyP in DNA by metal ions presumably accounts for the difference.展开更多
The study of the electrochemical behavior of water soluble porphyrins and metalloporphyrins is interesting and important in the fields of chemistry and biology. For instance, some metalloporphyrins are the catalysts o...The study of the electrochemical behavior of water soluble porphyrins and metalloporphyrins is interesting and important in the fields of chemistry and biology. For instance, some metalloporphyrins are the catalysts of redox reactions in chlorophyll. A previous paper reported the electrochemical properties of Zn-TPPS. This note discusses the voltammetric behavior of TMPyP and Zn-TMPyP.展开更多
基金the National Natural Science Foundation of China （No.20275022）.
文摘The interaction between calf thymus DNA （ctDNA） and Pd （Ⅱ） meso-tetralds- （4-N-methyl pyridiniumyl） porphyrin （Pd-TMPyP） was investigated by steady solid-substrate room temperature phosphorescence （SS-RTP）. The SS-RTP intensity and lifetime of Pd-TMPyP enhanced with the increasing ctDNA. The anion quenching experiment of Pd-TMPyP indicated that Pd-TMPyP intercalated into ctDNA, which was also approved by UV-Vis spectra. Keywords： Pd-TMPyP, ctDNA, SS-RTP, intercalation
基金the National Research Foundation of Korea(20011-0013534,2009-0076930) the National Natural Science Foundation of China(21265022)
文摘The effect of various metal ions on the DNA mediated energy transfer between simultaneously bound drugs was investigated using spectroscopic methods.It was found that addition of divalent metal ions(Mg^(2+),Ca^(2+),Mn^(2+),Co^(2+)and Ni^(2+))resulted in further decrease of the ethidium fluorescence intensity,while a small increase was observed in the TMPyP emission band,implying that the energy of excited ethidium was transferred to TMPyP.This DNA-mediated quenching efficiency between ethidium and TMPyP was significantly enhanced by the presence of all metal ions.Among the divalent metal ions,alkali earth metal ions and Mn^(2+)displayed higher quenching efficiencies than other transition metal ions.The distances required to permit the energy transfer between the two drugs in DNA were calculated as 68,66,62,48and 38in the presence of100μmol·L^(-1 )of Mg^(2+),Ca^(2+),Mn^(2+),Co^(2+)and Ni ^(2+)ion,respectively.The disturbed binding conformation of TMPyP in DNA by metal ions presumably accounts for the difference.
基金Project supported by the National Natural Science Foundation of China.
文摘The study of the electrochemical behavior of water soluble porphyrins and metalloporphyrins is interesting and important in the fields of chemistry and biology. For instance, some metalloporphyrins are the catalysts of redox reactions in chlorophyll. A previous paper reported the electrochemical properties of Zn-TPPS. This note discusses the voltammetric behavior of TMPyP and Zn-TMPyP.
