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亚洲黑熊四川亚种Ursus thibetanus mupinensis线粒体细胞色素C氧化酶亚基Ⅲ基因的克隆与初步分析 被引量:5
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作者 杜玉杰 黎云祥 +2 位作者 吴夏 陈瑜 侯万儒 《四川大学学报(自然科学版)》 CAS CSCD 北大核心 2007年第6期1363-1368,共6页
根据已报道的部分哺乳动物线粒体细胞色素C氧化酶(Cytochrome C Oxidase)亚基Ⅲ基因(mtCOXⅢ)的相关信息设计引物,运用PCR技术,首次从亚洲黑熊四川亚种(以下简称四川黑熊,Ursus thibetanus mupinensis)的肌肉组织总DNA中成功克隆了mtCO... 根据已报道的部分哺乳动物线粒体细胞色素C氧化酶(Cytochrome C Oxidase)亚基Ⅲ基因(mtCOXⅢ)的相关信息设计引物,运用PCR技术,首次从亚洲黑熊四川亚种(以下简称四川黑熊,Ursus thibetanus mupinensis)的肌肉组织总DNA中成功克隆了mtCOXⅢ基因的编码序列,并对其进行了初步分析.结果表明:四川黑熊mtCOXⅢ基因序列全长784 bp,ORF是783 bp,编码261个氨基酸的蛋白质,该蛋白的等电点为6.47,分子量为29840.6 Da.四川黑熊的mtCOXⅢ与已报道的部分哺乳动物的mtCOXⅢ基因具有很高的同源性.进一步分析发现,四川黑熊线粒体细胞色素C氧化酶亚基Ⅲ功能位点的位置、种类和数量均与已报道的部分哺乳动物具有很高的相似性. 展开更多
关键词 亚洲黑熊四川亚种(ursus thibetanus mupinensis) 线粒体 细胞色素C氧化酶亚基Ⅲ(mtCOXⅢ) 克隆 序列分析
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Blood Typing of Asiatic Black Bear (Ursus thibetanus)
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作者 Li Qin Chen Yucun +3 位作者 Zhao Fengyong Zhang Jiamin Zhang Shuhui Zhu Ziyan 《Animal Husbandry and Feed Science》 CAS 2017年第5期322-325,共4页
[Objective]The paper was to analyze whether Asiatic black bear( Ursus thibetanus) had different blood group systems. [Method]Whole blood samples were collected from 40 Asiatic black bears in Fujian Province,China. Tub... [Objective]The paper was to analyze whether Asiatic black bear( Ursus thibetanus) had different blood group systems. [Method]Whole blood samples were collected from 40 Asiatic black bears in Fujian Province,China. Tube method was used to detect antibodies in plasma,and antibody isotype was determined with 2-mercaptoethanol. Plasma was further analyzed by mass spectrometry. [Result] The plasma from four black bears had antibodies,possibly Ig M isoform,which could agglutinate RBCs from 30 bears. Blood samples from 10 bears were tested by human blood typing reagents. The results showed that four black bears had blood type like human type O,while six bears had like human type B. Mass spectrometry results demonstrated that plasma protein had extensive homology to serum albumin-like isoform 1 found in giant panda( Ailuropoda melanoleuca). [Conclusion]Asiatic black bear might have at least one blood group system with two blood types. If the sick bear needs blood transfusion,a cross-matching test is necessary. Moreover,giant panda might receive blood from Asiatic black bear in case of emergency. 展开更多
关键词 Asiatic BLACK BEAR (ursus thibetanus) BLOOD group system Cross MATCHING test TRANSFUSION medicine
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四川黑熊线粒体12S和16S rRNA基因的克隆及序列分析 被引量:6
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作者 吴夏 陈瑜 +2 位作者 彭正松 杨军 侯万儒 《四川师范大学学报(自然科学版)》 CAS CSCD 北大核心 2007年第3期402-405,共4页
利用哺乳动物线粒体基因的保守序列设计引物,采用PCR方法,首次从亚洲黑熊四川亚种(Ursusthibetanus mupinensis)的肌肉组织总DNA中扩增出了线粒体12S rRNA和16S rRNA基因并进行了序列测定及分析.结果表明,四川黑熊12S rRNA基因长965 bp;... 利用哺乳动物线粒体基因的保守序列设计引物,采用PCR方法,首次从亚洲黑熊四川亚种(Ursusthibetanus mupinensis)的肌肉组织总DNA中扩增出了线粒体12S rRNA和16S rRNA基因并进行了序列测定及分析.结果表明,四川黑熊12S rRNA基因长965 bp;16S rRNA基因长1 580 bp.通过进一步的序列分析表明,四川黑熊的12S rRNA和16S rRNA基因有较高的进化速率,与美洲黑熊、棕熊、北极熊、眼镜熊及大熊猫的相应基因相比较有较大差异,其中与美洲黑熊的同源性相对较高. 展开更多
关键词 亚洲黑熊四川亚种(ursus thibetanus mupinensis) 线粒体 12S RRNA 16S RRNA 序列分析
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四川黑熊RPS16亚基基因的克隆与序列分析 被引量:3
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作者 王婷 侯怡铃 +4 位作者 丁祥 孙冰 苏秀兰 李俊 侯万儒 《西华师范大学学报(自然科学版)》 2012年第4期350-355,共6页
根据已报道的部分哺乳动物RPS16基因的相关信息设计引物,运用RT-PCR技术,从四川黑熊的肌肉组织总RNA中对核糖体蛋白亚基RPS16基因的表达序列进行克隆、测序和分析.结果表明:四川黑熊RPS16基因的表达序列全长为481bp,开放阅读框(ORF)为44... 根据已报道的部分哺乳动物RPS16基因的相关信息设计引物,运用RT-PCR技术,从四川黑熊的肌肉组织总RNA中对核糖体蛋白亚基RPS16基因的表达序列进行克隆、测序和分析.结果表明:四川黑熊RPS16基因的表达序列全长为481bp,开放阅读框(ORF)为441bp,编码146个氨基酸,相对分子质量为16.4KDa,pI为10.81.拓扑预测显示含有6个类型的功能位点:1个依赖cAMP和cGMP的蛋白激酶磷酸化位点,3个蛋白激酶C磷酸化位点,1个酪蛋白激酶II磷酸化位点,2个N-糖基化位点,1个乙酰化位点及1个核糖体蛋白S9信号位点.进一步分析发现,四川黑熊RPS16基因与已报道的大熊猫、牛、野猪、人、小家鼠和褐家鼠等6个哺乳动物物种的表达序列及其编码的氨基酸序列有很高的相似性,说明RPS16基因具有高度的遗传稳定性和功能一致性. 展开更多
关键词 四川黑熊(ursus thibetanus mupinensis) RT—PCR RPS16 克隆 序列分析
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SEPARATING BROWN AND BLACK BEARS IN NORTHEAST CHINA USING TOOTH MEASUREMENTS
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作者 姜兆文 郑红 +1 位作者 王玉玺 张淑云 《Journal of Northeast Forestry University》 SCIE CAS CSCD 1995年第2期58-60,共3页
An expeditious method for ascertaining species between black bear (Selenarctos thibetanus G. cuiver) and brown bear (Ursus arctos Linnaeus) was developed using tooth measurements from previously identified specimens. ... An expeditious method for ascertaining species between black bear (Selenarctos thibetanus G. cuiver) and brown bear (Ursus arctos Linnaeus) was developed using tooth measurements from previously identified specimens. The measurement and analysis on 18 tooth measurement indexes (i.e. T1. Length of Pm1-Pm4, T2. L. of M1-M3, T3. M1L., T4. M1 width, T5. M2L., T6. M2W., T7.M3L., T8. M3W., T9. L. of C1 alveolus, T10. W. of C1 alveolus, T11. L. of C1-M2, T12. L. of Pm4 M2,T13.M1L., T14. M1W., T15. M2L., T16. M2W., T17.L. of C1 alveolus, T18. W. of C1 alveolus) of 59 skulls (black bears 25, brown bears 34) indicates that there are significant or the most significant difference between two species in every indexes, however T1, T9, T10, T17 and T18 are not suitable for species discriminator because of their high percent of overlap. The efficient species discriminators and standards (mm) are as follows T2(63.0), T3(22.0), T4(10.2), T5(23.8), T6(13.6), T7(18.0), T8(13.5), T11(110.0),T12(66.0), T13(20. 1), T14(15.8), T15(33.0) and T16(17.3). Those who are less than or equal to standard are classified to black bear, the others are brown bear. The method is very convenient and useful with high reliability. Their lowest accuracy percent are 95.0%, most of them over 97%. Different indexes will be chosen refer to the degree of specimen destroyed. 展开更多
关键词 BLACK BEAR (Selenarctos thibetanus) BROWN BEAR (ursus arctos) TOOTH measurement SEPARATING
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Resident Perceptions of Black Bear Management in South Central Kentucky
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作者 Hung-Ling Stella Liu Michael J. Bradley +1 位作者 I-Chun Nicky Wu James N. Maples 《Natural Resources》 2019年第7期271-283,共13页
The growth and sprawl of the American black bear (Ursus americanus) population have been observed and monitored in the past two decades, especially in south-central Kentucky. To better manage human-black bear interact... The growth and sprawl of the American black bear (Ursus americanus) population have been observed and monitored in the past two decades, especially in south-central Kentucky. To better manage human-black bear interaction and develop informed policy and best practices, the current study sought to 1) understand south-central Kentucky residents’ current knowledge of and previous experience with black bears, 2) investigate residents’ attitudes toward black bears and regulated hunting in wildlife management;and 3) identify residents’ level of acceptance of various black bear related management actions in south-central Kentucky. From February to April 2017, residents of London and Stearns districts (southeastern regions) of the Daniel Boone National Forest, a black bear habitat, were invited to participate in this study. The results from 139 completed surveys showed that residents lacked sufficient knowledge about black bear populations in the region and received minimal information regarding black bear management efforts. In comparison to education efforts and relocation, regulated black bear hunting could be a cost-effective option to promote responsible use of wildlife resources and black bear population control. This study provided lessons and recommendations for black bear management in south-central Kentucky and may be of utilization for any other wildlife conservation areas. 展开更多
关键词 Black Bear (ursus AMERICANUS ) WILDLIFE MANAGEMENT National Forest WILDLIFE Tourism Interpretation Environmental Education KENTUCKY HUNTING WILDLIFE RESOURCES Natural RESOURCES
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气候变化对喜马拉雅中部甘达基河流域濒危物种的影响预测
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作者 Raju RAI 张镱锂 +4 位作者 刘林山 Paras Bikram SINGH Basanta PAUDEL Bipin Kumar ACHARYA Narendra Raj KHANAL 《Journal of Resources and Ecology》 CSCD 2022年第2期173-185,共13页
甘达基河流域位于喜马拉雅山脉中部,是众多濒危野生物种的重要栖息地。然而气候变化使该流域的生态环境变得愈发脆弱。本研究利用最大熵物种分布模型(Max Ent)评估气候变化对喜马拉雅黑熊(Ursus thibetanus laniger)和印度花豹(Panthera... 甘达基河流域位于喜马拉雅山脉中部,是众多濒危野生物种的重要栖息地。然而气候变化使该流域的生态环境变得愈发脆弱。本研究利用最大熵物种分布模型(Max Ent)评估气候变化对喜马拉雅黑熊(Ursus thibetanus laniger)和印度花豹(Panthera pardus fusca)等濒危物种空间分布变化的潜在影响。研究基于物种出没地点、生物气候和地形(海拔、坡度和坡向)等数据拟合模拟并预测物种在目前与未来的潜在分布情况。研究结果显示,目前喜马拉雅黑熊的高度适宜区面积约为1642 km^(2),占流域面积的5.01%,预计至2050年,其高度适宜区面积在甘达基河流域内将会增加约51 km^(2);印度花豹的高度适宜区面积约为3999 km^(2),占流域面积的12.19%,预计至2050年可能会增加到4806 km^(2)。喜马拉雅黑熊的栖息地面积可能会在研究区域的东部(伯塞里、塔托潘尼和班塞以北)增加,而在东部(颂当、切坎帕)、西部(布尔提邦和波邦)和北部(桑波切、玛南、切坎帕)减少;印度花豹的栖息地面积则将在研究区域东南部(班塞)、西部部分地区(赫里乔尔和桑迪哈克北部)和西北部(桑波切)增加,而在研究区域的东部、南部和其他西部地区减少。研究同时指出,海拔、Bio 15(季节性降水变化)和Bio 16(最湿润季度降水量)等环境因素对喜马拉雅黑熊的栖息地变化影响较大,而Bio 13(最湿润月降水量)和Bio 15(季节性降水变化)对印度花豹的栖息地变化影响较大。总之,这两个物种的栖息地在不同海拔下均会受到气候变化的影响,需要加强对该区域内濒危物种的保护力度。 展开更多
关键词 气候变化 物种栖息地变化 喜马拉雅黑熊(ursus thibetanus laniger) 印度花豹(Panthera pardus fusca) 甘达基河流域
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