Kaempferol(KA),as one of the flavonoids,has extensive pharmacological properties.However,the poor solubility of KA severely limits its clinical application.In our study,the kaempferol phospholipid complex(KA-PC)has be...Kaempferol(KA),as one of the flavonoids,has extensive pharmacological properties.However,the poor solubility of KA severely limits its clinical application.In our study,the kaempferol phospholipid complex(KA-PC)has been prepared by solvent evaporation for the enhancement of the bioavailability of KA.KA-PC was verified by scanning electron microscope characterization methods.Drug loading,solubility and long-term stability were measured.The characterization results showed that KA-PC was formed through the intermolecular interaction between KA and phospholipids.The solubility of KA-PC in water was 189 times higher than that of KA,and the solubility in n-octanol was also significantly improved.Besides,pharmacodynamic studies showed that KA-PC can significantly reduce the level of serum uric acid in mice without causing renal injury.This study expanded the clinical application of KA by preparing KA-PC.展开更多
[Objectives]The study aims to discuss the effects of addition of arginine and glutamic acid or soybean phospholipid,vitamin E and yeast selenium in diet on the slaughter performance and meat quality of long(white)...[Objectives]The study aims to discuss the effects of addition of arginine and glutamic acid or soybean phospholipid,vitamin E and yeast selenium in diet on the slaughter performance and meat quality of long(white)×large(York)binary hybrid pigs.[Methods]27 long×large castrated hybrid boars with the body weight of(54.4±0.15)kg were randomly divided into 3 groups,with 3 replicates per group and 3 pigs per replicate.Group A was the control group,in which the pigs were fed basal diet;in group B,0.8%arginine and 0.60%glutamate were added to the basal diet;in group C,75 g of soybean phospholipid,20 g of vitamin E and 8 g of yeast selenium were added to every 100 kg of the basal diet.The trial period was 60 d.After the experiment was ended,one test pig with similar body weight was selected from each replicate for slaughter and meat determination.[Results]The average weight gain and eye muscle area of the pigs in group B were significantly higher than those in group C(P<0.05),and also showed an increasing trend compared with group A,but there was no statistically significant difference(P>0.05);there was no significant difference between group B or C and group A in the average weight gain and eye muscle area(P>0.05).There was no significant difference in other slaughter performance between the three groups(P>0.05).Besides,there was also no significant difference in the content of various amino acids,total amino acids and total umami amino acids between the three groups(P>0.05).The inosine content in the longissimus dorsi muscle and muscle cooking loss of binary hybrid pigs in group C were significantly better than those in group B(P<0.05),and also had a tendency to be better than those in group A,but there was no significant difference(P>0.05);there was no significant difference between group B or C and group A in the inosine content and muscle cooking loss of the pigs(P>0.05).In addition,there was no significant difference in other meat traits and chemical composition of the longissimus dorsi muscle between group B or C and group A(P>0.05).[Conclusions]The addition of arginine and glutamic acid or soybean phospholipid,vitamin E and yeast selenium in diet had no significant effect on the growth rate,slaughter performance and meat traits of long×large binary hybrid pigs.展开更多
背景:近年来,有研究发现部分中药单体通过激活细胞自噬可缓解皮瓣氧化应激与细胞凋亡,促进皮瓣血管再生,防治皮瓣坏死。目的:综述中药单体调控细胞自噬防治皮瓣坏死的研究进展。方法:分别以“中药、细胞自噬、皮瓣”与“Traditional Chi...背景:近年来,有研究发现部分中药单体通过激活细胞自噬可缓解皮瓣氧化应激与细胞凋亡,促进皮瓣血管再生,防治皮瓣坏死。目的:综述中药单体调控细胞自噬防治皮瓣坏死的研究进展。方法:分别以“中药、细胞自噬、皮瓣”与“Traditional Chinese Medicine (TCM),Autophagy,Skin flaps”为中英文检索词,由第一作者检索2010年1月至2022年10月发表在中国知网与PubMed数据库的相关文献,文献初筛共检索到相关文献196篇,以纳入和排除标准进行筛选,通过阅读文献标题与摘要进行质量评估,最终归纳整理55篇文献进行综述。结果与结论:(1)细胞自噬的调控是由AMPK/mTOR和PI3K/AKT等信号通路介导的,激活细胞自噬可缓解皮瓣氧化应激与细胞凋亡,促进皮瓣血管再生,防治皮瓣坏死。(2)中药单体中的萜类化合物(桦木酸、穿心莲内酯、三七三萜和梓醇)、酚类化合物(白藜芦醇、姜黄素和天麻素)、酚酸类化合物(丹酚酸B)及类固醇类化合物(拟人参皂甙F11)可通过调控相关信号通路激活细胞自噬来缓解皮瓣氧化应激与细胞凋亡,促进皮瓣血管再生,促皮瓣存活。(3)文章通过总结中药单体调控细胞自噬防治皮瓣坏死的研究进展,为临床上中药防治皮瓣坏死、促进皮瓣愈合提供参考与理论依据。展开更多
基金funded by Young and Middle Aged Teachers’Career Development Support Project of Shenyang Pharmaceutical University(ZQN2019005).
文摘Kaempferol(KA),as one of the flavonoids,has extensive pharmacological properties.However,the poor solubility of KA severely limits its clinical application.In our study,the kaempferol phospholipid complex(KA-PC)has been prepared by solvent evaporation for the enhancement of the bioavailability of KA.KA-PC was verified by scanning electron microscope characterization methods.Drug loading,solubility and long-term stability were measured.The characterization results showed that KA-PC was formed through the intermolecular interaction between KA and phospholipids.The solubility of KA-PC in water was 189 times higher than that of KA,and the solubility in n-octanol was also significantly improved.Besides,pharmacodynamic studies showed that KA-PC can significantly reduce the level of serum uric acid in mice without causing renal injury.This study expanded the clinical application of KA by preparing KA-PC.
基金Supported by Self-funded Project of Agricultural Science and Technology of Guangxi(Z2022114).
文摘[Objectives]The study aims to discuss the effects of addition of arginine and glutamic acid or soybean phospholipid,vitamin E and yeast selenium in diet on the slaughter performance and meat quality of long(white)×large(York)binary hybrid pigs.[Methods]27 long×large castrated hybrid boars with the body weight of(54.4±0.15)kg were randomly divided into 3 groups,with 3 replicates per group and 3 pigs per replicate.Group A was the control group,in which the pigs were fed basal diet;in group B,0.8%arginine and 0.60%glutamate were added to the basal diet;in group C,75 g of soybean phospholipid,20 g of vitamin E and 8 g of yeast selenium were added to every 100 kg of the basal diet.The trial period was 60 d.After the experiment was ended,one test pig with similar body weight was selected from each replicate for slaughter and meat determination.[Results]The average weight gain and eye muscle area of the pigs in group B were significantly higher than those in group C(P<0.05),and also showed an increasing trend compared with group A,but there was no statistically significant difference(P>0.05);there was no significant difference between group B or C and group A in the average weight gain and eye muscle area(P>0.05).There was no significant difference in other slaughter performance between the three groups(P>0.05).Besides,there was also no significant difference in the content of various amino acids,total amino acids and total umami amino acids between the three groups(P>0.05).The inosine content in the longissimus dorsi muscle and muscle cooking loss of binary hybrid pigs in group C were significantly better than those in group B(P<0.05),and also had a tendency to be better than those in group A,but there was no significant difference(P>0.05);there was no significant difference between group B or C and group A in the inosine content and muscle cooking loss of the pigs(P>0.05).In addition,there was no significant difference in other meat traits and chemical composition of the longissimus dorsi muscle between group B or C and group A(P>0.05).[Conclusions]The addition of arginine and glutamic acid or soybean phospholipid,vitamin E and yeast selenium in diet had no significant effect on the growth rate,slaughter performance and meat traits of long×large binary hybrid pigs.
文摘背景:近年来,有研究发现部分中药单体通过激活细胞自噬可缓解皮瓣氧化应激与细胞凋亡,促进皮瓣血管再生,防治皮瓣坏死。目的:综述中药单体调控细胞自噬防治皮瓣坏死的研究进展。方法:分别以“中药、细胞自噬、皮瓣”与“Traditional Chinese Medicine (TCM),Autophagy,Skin flaps”为中英文检索词,由第一作者检索2010年1月至2022年10月发表在中国知网与PubMed数据库的相关文献,文献初筛共检索到相关文献196篇,以纳入和排除标准进行筛选,通过阅读文献标题与摘要进行质量评估,最终归纳整理55篇文献进行综述。结果与结论:(1)细胞自噬的调控是由AMPK/mTOR和PI3K/AKT等信号通路介导的,激活细胞自噬可缓解皮瓣氧化应激与细胞凋亡,促进皮瓣血管再生,防治皮瓣坏死。(2)中药单体中的萜类化合物(桦木酸、穿心莲内酯、三七三萜和梓醇)、酚类化合物(白藜芦醇、姜黄素和天麻素)、酚酸类化合物(丹酚酸B)及类固醇类化合物(拟人参皂甙F11)可通过调控相关信号通路激活细胞自噬来缓解皮瓣氧化应激与细胞凋亡,促进皮瓣血管再生,促皮瓣存活。(3)文章通过总结中药单体调控细胞自噬防治皮瓣坏死的研究进展,为临床上中药防治皮瓣坏死、促进皮瓣愈合提供参考与理论依据。
