目的研究重组人促红细胞生成素(reCombinant human erythropoietin,r-huEPO)对颅脑创伤患者血清神经元特异性烯醇化酶(neuron-specificenolase,NSE)表达的影响,评价其神经保护作用。方法纳入住院治疗的颅脑创伤患者32例,随机分配到对照...目的研究重组人促红细胞生成素(reCombinant human erythropoietin,r-huEPO)对颅脑创伤患者血清神经元特异性烯醇化酶(neuron-specificenolase,NSE)表达的影响,评价其神经保护作用。方法纳入住院治疗的颅脑创伤患者32例,随机分配到对照组与治疗组,治疗组于规定时间皮下注射r-huEPO。对照组不予r-huEPO处理,其他治疗两组相同。比较治疗后两组患者GCS评分、颅内压及脑水肿变化情况;两组均在研究时间静脉采血,利用双抗体夹心酶联免疫(ELISA)法分析各组NSE变化。结果从临床征象比较,治疗组患者较对照组恢复较好;两组患者血清NSE均随时间变化表达不同,治疗组血清NSE含量低于对照组,差异有统计学意义(P<0.05)。结论NSE是较为敏感特异的神经元和神经胶质细胞损伤的指标,利用r-huEPO进行红细胞动员,可通过各种机制抑制颅脑创伤后的继发病理损害及其过度炎症反应,对神经系统损伤具有保护作用。展开更多
目的:研究重组人促红细胞生成素(recombinant human erythropoietin,r-huEPO)对颅脑创伤患者血清S-100β蛋白和IL-10的影响,评价其临床保护作用。方法:31例患者随机分为对照组与治疗组,其中治疗组于规定时间皮下注射r-huEPO,对照组不予r...目的:研究重组人促红细胞生成素(recombinant human erythropoietin,r-huEPO)对颅脑创伤患者血清S-100β蛋白和IL-10的影响,评价其临床保护作用。方法:31例患者随机分为对照组与治疗组,其中治疗组于规定时间皮下注射r-huEPO,对照组不予r-huEPO处理,其他治疗两组相同。两组均在伤后第1、4、7、10、14天静脉采血,利用ELISA法测定S-100β蛋白和IL-10含量,并与患者临床征象作比较。结果:治疗组患者颅内压及脑水肿较对照组减轻,且GCS评分升高;血清S-100β蛋白含量低于对照组,IL-10含量高于对照组,差异均有统计学意义(P<0.05)。结论:利用r-huEPO进行红细胞动员,可通过各种机制抑制颅脑创伤后继发的病理损害及其过度炎症反应,对颅脑创伤具有神经保护作用。展开更多
Using electron spin resonance (ESR) spin labeling technique, we have studied the conformation of sulfhydryl groups(-SH) binding sites in membrane proteins and membrane fluidity of red blood cells (RBCs) from two group...Using electron spin resonance (ESR) spin labeling technique, we have studied the conformation of sulfhydryl groups(-SH) binding sites in membrane proteins and membrane fluidity of red blood cells (RBCs) from two groups of patients with anemia of chronic renal failure (ACRF). One of the groups is composed of patients who were untreated with recombinant human erythropoietin(r-HuEPO), and the other is composed of patients who were treated with r-HuEPO. The results indicated: 1) the conformation of SH group binding site in RBC membrane proteins from former group was different from those of health people. 2)the fluidity in the region near the surface of RBC membrane from former group was lower than those of healthy people. 3) However, the above biophysical properties of RBC membrane from later group were normal. We concluded that RBC membrane in patients with ACRF was abnormal, and the treatment of r-HuEPO may promote the production of normal RBCs, thus ameliorate the biophysical properties of RBCs from the patients with ACRF.展开更多
Objective: To explore the regulative effect on Survivin of r-HuEPO after traumatic brain injury (TBI) in rats, and understand the neuroprotection mechanisms of r-HuEPO. Methods: Seventy-eight adult Wistar rats were ra...Objective: To explore the regulative effect on Survivin of r-HuEPO after traumatic brain injury (TBI) in rats, and understand the neuroprotection mechanisms of r-HuEPO. Methods: Seventy-eight adult Wistar rats were randomly divided into sham operation group (n = 6), TBI group (n = 36) and r-HuEPO group (n = 36). The experimental TBI model was created by Feeney’s method. Samples were obtained after injury for measuring apoptosis of cells by Epics XL Flow Cytometer. Immunochemical method was performed for inspection of expressions of Survivin and NF-κB proteins. Results: Compared to the sham group, the number of apoptotic cells and Survivin, NF-κB immunopositive cells was significantly increased in the injured brain after TBI (P < 0.01). R-HuEPO significantly increased the expression of survivin and NF-κB, but decreased the apoptotic rates. Conclusion: Increased expression of NF-κB by r-HuEPO may play important role in regulating Survivin level, inhibiting neuronal apoptosis in cortex and exerting protective function to neurons.展开更多
文摘目的研究重组人促红细胞生成素(reCombinant human erythropoietin,r-huEPO)对颅脑创伤患者血清神经元特异性烯醇化酶(neuron-specificenolase,NSE)表达的影响,评价其神经保护作用。方法纳入住院治疗的颅脑创伤患者32例,随机分配到对照组与治疗组,治疗组于规定时间皮下注射r-huEPO。对照组不予r-huEPO处理,其他治疗两组相同。比较治疗后两组患者GCS评分、颅内压及脑水肿变化情况;两组均在研究时间静脉采血,利用双抗体夹心酶联免疫(ELISA)法分析各组NSE变化。结果从临床征象比较,治疗组患者较对照组恢复较好;两组患者血清NSE均随时间变化表达不同,治疗组血清NSE含量低于对照组,差异有统计学意义(P<0.05)。结论NSE是较为敏感特异的神经元和神经胶质细胞损伤的指标,利用r-huEPO进行红细胞动员,可通过各种机制抑制颅脑创伤后的继发病理损害及其过度炎症反应,对神经系统损伤具有保护作用。
文摘目的:研究重组人促红细胞生成素(recombinant human erythropoietin,r-huEPO)对颅脑创伤患者血清S-100β蛋白和IL-10的影响,评价其临床保护作用。方法:31例患者随机分为对照组与治疗组,其中治疗组于规定时间皮下注射r-huEPO,对照组不予r-huEPO处理,其他治疗两组相同。两组均在伤后第1、4、7、10、14天静脉采血,利用ELISA法测定S-100β蛋白和IL-10含量,并与患者临床征象作比较。结果:治疗组患者颅内压及脑水肿较对照组减轻,且GCS评分升高;血清S-100β蛋白含量低于对照组,IL-10含量高于对照组,差异均有统计学意义(P<0.05)。结论:利用r-huEPO进行红细胞动员,可通过各种机制抑制颅脑创伤后继发的病理损害及其过度炎症反应,对颅脑创伤具有神经保护作用。
文摘Using electron spin resonance (ESR) spin labeling technique, we have studied the conformation of sulfhydryl groups(-SH) binding sites in membrane proteins and membrane fluidity of red blood cells (RBCs) from two groups of patients with anemia of chronic renal failure (ACRF). One of the groups is composed of patients who were untreated with recombinant human erythropoietin(r-HuEPO), and the other is composed of patients who were treated with r-HuEPO. The results indicated: 1) the conformation of SH group binding site in RBC membrane proteins from former group was different from those of health people. 2)the fluidity in the region near the surface of RBC membrane from former group was lower than those of healthy people. 3) However, the above biophysical properties of RBC membrane from later group were normal. We concluded that RBC membrane in patients with ACRF was abnormal, and the treatment of r-HuEPO may promote the production of normal RBCs, thus ameliorate the biophysical properties of RBCs from the patients with ACRF.
文摘Objective: To explore the regulative effect on Survivin of r-HuEPO after traumatic brain injury (TBI) in rats, and understand the neuroprotection mechanisms of r-HuEPO. Methods: Seventy-eight adult Wistar rats were randomly divided into sham operation group (n = 6), TBI group (n = 36) and r-HuEPO group (n = 36). The experimental TBI model was created by Feeney’s method. Samples were obtained after injury for measuring apoptosis of cells by Epics XL Flow Cytometer. Immunochemical method was performed for inspection of expressions of Survivin and NF-κB proteins. Results: Compared to the sham group, the number of apoptotic cells and Survivin, NF-κB immunopositive cells was significantly increased in the injured brain after TBI (P < 0.01). R-HuEPO significantly increased the expression of survivin and NF-κB, but decreased the apoptotic rates. Conclusion: Increased expression of NF-κB by r-HuEPO may play important role in regulating Survivin level, inhibiting neuronal apoptosis in cortex and exerting protective function to neurons.
