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表面活性剂辅助的甲硫氨酸和色氨酸叶面喷施对芥蓝生长及菜薹芥子油苷组分和含量的影响 被引量:2
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作者 腊贵晓 孔海民 +1 位作者 方萍 杨铁钢 《中国生态农业学报》 CAS CSCD 北大核心 2014年第4期439-446,共8页
采用盆栽试验,研究了脂肪族芥子油苷和吲哚族芥子油苷的合成前体甲硫氨酸和色氨酸单独及配合表面活性剂叶面喷施对芥蓝(Brassica alboglabra L.)生长及菜薹中芥子油苷组分和含量的影响。结果表明,各处理对芥蓝生长指标及芥子油苷组分无... 采用盆栽试验,研究了脂肪族芥子油苷和吲哚族芥子油苷的合成前体甲硫氨酸和色氨酸单独及配合表面活性剂叶面喷施对芥蓝(Brassica alboglabra L.)生长及菜薹中芥子油苷组分和含量的影响。结果表明,各处理对芥蓝生长指标及芥子油苷组分无显著影响,在芥蓝菜薹中均检测到7种脂肪族芥子油苷和4种吲哚族芥子油苷。与喷施清水对照相比,甲硫氨酸、色氨酸单独叶面喷施及表面活性剂辅助的色氨酸叶面喷施,对芥蓝菜薹总芥子油苷含量、总脂肪族芥子油苷含量和总吲哚族芥子油苷含量均无显著影响。表面活性剂辅助下甲硫氨酸叶面喷施处理中菜薹总芥子油苷和总脂肪族芥子油苷含量比清水对照处理分别提高28.5%和31.9%,其中在11种芥子油苷中脂肪族芥子油苷3-丁烯基芥子油苷含量提高幅度最大,达到44.3%,同时表面活性剂辅助下甲硫氨酸叶面喷施显著提高了芥蓝菜薹全硫含量并降低了氮硫比(N/S)。本试验结果表明,有效供给甲硫氨酸能明显促进脂肪族芥子油苷的合成和积累,而辅之以表面活性剂能增进叶面对甲硫氨酸的吸收效果,其影响机理还有待进一步研究。 展开更多
关键词 芥蓝(Brassica alboglabraL )芥子油 甲硫氨酸色氨酸表面活性剂 氮硫比(N S)
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Effects of Drought Stress Simulated by PEG on Glucosinolates Content in Arabidopsis thaliana 被引量:5
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作者 孙晶 杨今朝 于涛 《Agricultural Science & Technology》 CAS 2010年第3期20-24,共5页
[Objective] The paper aimed to study effects of drought stress simulated by PEG on glucosinolates content in Arabidopsis thaliana.[Method] Drought stress was simulated by PEG-6000,ecological seeds of Arabidopsis thali... [Objective] The paper aimed to study effects of drought stress simulated by PEG on glucosinolates content in Arabidopsis thaliana.[Method] Drought stress was simulated by PEG-6000,ecological seeds of Arabidopsis thaliana were cultivated by the control group and drought treatment group respectively,Physical signs of Arabidopsis thaliana and contents of glucosinolates were determined after 0,4,5,6,7 d treatment.[Result] The results showed that leaf water content of rosette leaves was obviously decreased,leaf relative conductivity (characterized by membrane permeability) and the concentration of MDA increased,the extent of damage increased with the increased time.Content of total glucosinolate,aliphatic glucosinolate and indole glucosinolate increased got their maximum after 5 days treatment,and rapidly decreased after 6 and 7 days of treatment,even much lower than the control group.Each kind of glucosinolate changed with difference from each other.4MSOB which made the most proportion of the total glucosinolate changed consistently with the total glucosinolate and difference significant.As a whole,aliphatic glucosinolates were more sensitive to drought than indole glucosinolate.The proportion of some kind glucosinolate,like 4MSOB varied had correlation with the content change.[Conclusion] Drought stress had an effects on the contents of total glucosinolate,aliphatic glucosinolate and indole glucosinolate,which made the glucosinolate participated in defense response of plant to the outside of drought stress,but long-term drought stress in leaves was not conducive to the accumulation of glucosinolates. 展开更多
关键词 Arabidopsis thaliana GLUCOSINOLATE DROUGHT
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Viability of Stigmas of Pistils in Winter Rape: Peculiarity of Samples with Different Origins
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作者 Natalia Anatoliivna Glukhova 《Journal of Environmental Science and Engineering(B)》 2013年第6期383-387,共5页
The researches have been carried out in 2011-2012, in the experimental fields of Yuryev Plant Production Institute Kharkiv, Ukraine. The forms of "00" type (a low content of erucic acid and glucosinolates) and sel... The researches have been carried out in 2011-2012, in the experimental fields of Yuryev Plant Production Institute Kharkiv, Ukraine. The forms of "00" type (a low content of erucic acid and glucosinolates) and self-pollinated lines--110M-10, 127M-10, 132M-10 were studied; the forms of"0+" type--selfed lines 124-10, 221-10, 305-10; the forms of"+0" type--cultivars Victor, Janus, Emerald; forms of "++" type--cultivars Fyedorovskiy, Marens, Uspikh, as well as, self-incompatible forms 5C, 15C-10, 32C-10 and the original sterile form of mutant origin were used. Viability in stigmas of the pistil lasted 3-7 days, in the forms of "+0", "++" type, 7-15 days in the cultivars and forms of "0+", "00" type, 14-21 days in the self-incompatible and sterile forms. A fast loss of sensitivity to pollen by pistil's stigma was observed in the forms of"+0" and "++" types. On the contrary, in self-incompatible and sterile forms was observed a gradual increase of sensitivity of pistil's stigma to pollination and then its gradual fall. A maximum of sensitivity of a pistil's stigma to pollen in "00" and "0+" forms was on the 3rd-6th day after castration. 展开更多
关键词 Winter rape erucic acid GLUCOSINOLATES pistil viability ability to seed set.
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板蓝根正丁醇部位抗病毒活性组分及相关化学成分研究 被引量:10
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作者 何立巍 杨婧妍 侯宪邦 《中草药》 CAS CSCD 北大核心 2017年第14期2843-2849,共7页
目的研究板蓝根Isatidis Radix正丁醇部位的抗病毒活性组分及相关成分。方法利用溶剂法及色谱法提取分离板蓝根正丁醇部位各化学组分,MTT法体外筛选各组分抗单纯疱疹病毒1(HSV-1)活性,利用UPLC-Q-TOF-MS检测正丁醇部位及各组分的化学成... 目的研究板蓝根Isatidis Radix正丁醇部位的抗病毒活性组分及相关成分。方法利用溶剂法及色谱法提取分离板蓝根正丁醇部位各化学组分,MTT法体外筛选各组分抗单纯疱疹病毒1(HSV-1)活性,利用UPLC-Q-TOF-MS检测正丁醇部位及各组分的化学成分,运用Markview软件及Excel软件进行各组分差异性成分分析,根据统计结果得到抗病毒相关的化学成分。结果按照化学成分的相似性,利用色谱法将板蓝根正丁醇部位分为20个组分,其中10个组分具有明显抗病毒活性。UPLC-Q-TOF-MS检测到正丁醇部位中的44个化学成分,并明确了它们的化学结构,在各化学组分的分析检测及差异性分析中,发现了生物碱类、有机酸、糖和氨基酸衍生物共15个化学成分与抗病毒活性相关;尤其是1-甲氧基-3-甲醛、3-(3′,5′-二甲氧基-4′-羟基)-2-吲哚酮、1-甲氧基-3-吲哚酮、3-(甲基硫代)芥子油苷、氨基葡萄糖-6-磷酸酯在活性组分中量较高。结论板蓝根正丁醇部位以吲哚类生物碱、有机酸、核苷及氨基酸类成分为主,其中的单吲哚类、硫苷类及糖和氨基酸衍生物与抗病毒活性密切相关。 展开更多
关键词 板蓝根 抗病毒 UPLC-Q-TOF-MS 1-甲氧基-3-甲醛 3-(3′ 5′-二甲氧基-4′-羟基)-2-吲哚酮 3-(甲基硫代)芥子油
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Effects of glucose and gibberellic acid on glucosinolate content and antioxidant properties of Chinese kale sprouts 被引量:3
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作者 Hui-ying MIAO Meng-yu WANG +3 位作者 Jia-qi CHANG Han TAO Bo SUN Qiao-mei WANG 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2017年第12期1093-1100,共8页
Glucosinolates, anthocyanins, total phenols, and vitamin C, as well as antioxidant capacity, were investigated in Chinese kale sprouts treated with both glucose and gibberellic acid(GA_3). The combination of 3%(0.0... Glucosinolates, anthocyanins, total phenols, and vitamin C, as well as antioxidant capacity, were investigated in Chinese kale sprouts treated with both glucose and gibberellic acid(GA_3). The combination of 3%(0.03 g/ml) glucose and 5 μmol/L GA_3 treatment was effective in increasing glucosinolate content while glucose or GA_3 treatment alone did not influence significantly almost all individual glucosinolates or total glucosinolates. The total phenolic content and antioxidant activity of Chinese kale sprouts were enhanced by combined treatment with glucose and GA_3, which could be useful in improving the main health-promoting compounds and antioxidant activity in Chinese kale sprouts. 展开更多
关键词 GLUCOSINOLATE ANTIOXIDANT GLUCOSE Gibberellic acid Chinese kale sprouts
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