Soluble receptor for advanced glycation end products(sRAGE)acts as a decoy sequestering of RAGE ligands,thus preventing the activation of the ligand-RAGE axis linking human diseases.However,the molecular mechanisms un...Soluble receptor for advanced glycation end products(sRAGE)acts as a decoy sequestering of RAGE ligands,thus preventing the activation of the ligand-RAGE axis linking human diseases.However,the molecular mechanisms underlying sRAGE remain unclear.In this study,THP-1 monocytes were cultured in normal glucose(NG,5.5 mmol/L)and high glucose(HG,15 mmol/L)to investigate the effects of diabetesrelevant glucose concentrations on sRAGE and interleukin-1β(IL-1β)secretion.The modulatory effects of epigallocatechin gallate(EGCG)in response to HG challenge were also evaluated.HG enhanced intracellular reactive oxygen species(ROS)generation and RAGE expression.The secretion of sRAGE,including esRAGE and cRAGE,was reduced under HG conditions,together with the downregulation of a disintegrin and metallopeptidase 10(ADAM10)and nuclear factor erythroid 2-related factor 2(Nrf2)nuclear translocation.Mechanistically,the HG effects were counteracted by siRAGE and exacerbated by siNrf2.Chromatin immunoprecipitation results showed that Nrf2 binding to the ADAM10 promoter and HG interfered with this binding.Our data reinforce the notion that RAGE and Nrf2 might be sRAGE-regulating factors.Under HG conditions,the treatment of EGCG reduced ROS generation and RAGE activation.EGCG-stimulated cRAGE release was likely caused by the upregulation of the Nrf2-ADAM10 pathway.EGCG inhibited HG-mediated NLRP3 inflammasome activation at least partly by stimulating sRAGE,thereby reducing IL-1βrelease.展开更多
为探究表没食子儿茶素没食子酸酯-牛骨蛋白(Epigallocatechin-3-gallate-Bovine bone protein,EGCG-BBP)对乳化肉制品蛋白结构及贮藏氧化稳定性的影响,本文研究不同EGCG-BBP添加量对生肉糜中肌原纤维蛋白(Myofibrillar protein,MP)的理...为探究表没食子儿茶素没食子酸酯-牛骨蛋白(Epigallocatechin-3-gallate-Bovine bone protein,EGCG-BBP)对乳化肉制品蛋白结构及贮藏氧化稳定性的影响,本文研究不同EGCG-BBP添加量对生肉糜中肌原纤维蛋白(Myofibrillar protein,MP)的理化性质、结构特性以及对肉丸氧化特性的影响。结果表明:当EGCG-BBP添加量为0.8%时,肉糜中MP的巯基含量最高,达4.06 nmol/mg蛋白,且羰基含量及表面疏水性最低,能够有效提升乳化肉制品的抗氧化能力。由红外光谱分析表明,与未添加EGCG-BBP组相比,添加共价物肉糜中MP的酰胺A带峰值所对应的波数明显增大,说明MP的二级结构会随之发生改变;荧光光谱显示,随贮藏时间延长,对照组中MP的最强荧光波长发生显著红移,但随EGCG-BBP浓度的增加,红移程度显著降低,表明添加EGCGBBP能够改变MP的三级结构。此外,乳化肉丸贮藏过程中的氧化指标分析表明,添加0.8%EGCG-BBP能显著降低肉丸的过氧化值(PV)和硫代巴比妥酸值(TBARs),从而提高其氧化稳定性。综上所述,EGCG-BBP能够显著改变MP的二、三级结构,且具有良好的抗氧化性能,在提升乳化肉制品品质方面具有很大的应用潜力,为肉品抗氧化型乳化剂的应用提供新的选择。展开更多
（-）-Epigallocatechin gallate （EGCG）, a major polyphenolic constituent of green tea, can inhibit activity of specific receptor tyrosine kinases （RTKs） and related downstream signal transduction pathways, resultin...（-）-Epigallocatechin gallate （EGCG）, a major polyphenolic constituent of green tea, can inhibit activity of specific receptor tyrosine kinases （RTKs） and related downstream signal transduction pathways, resulting in the control of unwanted cell proliferation. The epidermal growth factor receptor （EGFR） signaling pathway is one of the most important pathways that regulates growth, survival,proliferation and differentiation in mammalian cells. This review addresses the effects of EGCG on some protein factors involved in the EGFR signaling pathway in a direct or indirect manner. Based on our understanding of the interaction between EGCG and these factors, and based on their structures, EGCG could be used as a lead compound for designing and synthesizing novel drugs with significant biological activity.展开更多
The fresh leaves of China green tea, Camellia sinensis, were collected from Fuyang, Zhejiang Province, China, in April. The tea polyphenols was extracted by four different methods （homogenized with distilled water at...The fresh leaves of China green tea, Camellia sinensis, were collected from Fuyang, Zhejiang Province, China, in April. The tea polyphenols was extracted by four different methods （homogenized with distilled water at room temperature, homogenized with 0.3% citric acid （w/v） at room temperature, 5- min boiling and homogenized with distilled water at room temperature, homogenized with 85℃ distilled water）, and after preserving at room temperature, the change of the Epigallocatechin gallate （EGCG） contents of the extracts was investigated. Results indicated that the EGCG content of homogenate extracted with 85℃ distilled water was the highest before the extract was preserved, followed by that of the extract homogenized with 0.3% citric acid at room temperature. During preservation, EGCG content changed obviously. The EGCG contents of homogenates extracted with distilled water at room temperature and 85℃ distilled water declined quickly and separately reduced to 21.52% and 54.6% of their initial contents after preservation for 12 h. The EGCG contents extracted by 0.3% citric acid （w/v） solvent at room temperature and 5- min boiling/homogenized with distilled water at room temperature declined relatively slowly ,and separately reduced to 76.9% and 85.16% of their initial contents after preservation for 12 h. It was also found that the citric acid can prevent the degradation of EGCG and the extract solution color is light green展开更多
目的:评价表没食子儿茶素没食子酸酯(EGCG)及其甲基化修饰物(EGCG-3Me)改性粘接剂对根管牙本质粘接界面稳定性的作用。方法:将质量浓度为400μg/ml的EGCG及EGCG-3Me添加到全酸蚀粘接剂Single Bond 2(SB2)中,制备改性粘接剂E-SB2及E3-SB2...目的:评价表没食子儿茶素没食子酸酯(EGCG)及其甲基化修饰物(EGCG-3Me)改性粘接剂对根管牙本质粘接界面稳定性的作用。方法:将质量浓度为400μg/ml的EGCG及EGCG-3Me添加到全酸蚀粘接剂Single Bond 2(SB2)中,制备改性粘接剂E-SB2及E3-SB2,SB2为对照组。激光共聚焦显微镜和分光光度法检测改性粘接剂抗粪肠球菌的性能。微拉曼光谱仪检测粘接剂双键转化率。制备纤维桩粘接试件,用于即刻和老化后的微推出实验。结果:改性粘接剂可以抑制粪肠球菌生物膜形成,且EGCG-3Me作用更显著。改性粘接剂与SB2的双键转化率和即刻微推出粘接强度差异无显著性(P>0.05)。老化后改性粘接剂的微推出粘接强度显著高于SB2(P<0.05)。结论:EGCG和EGCG-3Me改性的粘接剂均可抑制粪肠球菌增殖并提高树脂-根管牙本质粘接界面稳定性,EGCG-3Me抗菌性能较佳。展开更多
Surface properties are considered to be important factors in addressing proper functionalities.In this paper,a multifunctional mussel-inspired coating was prepared via the direct copolymerization of epigallocatechin g...Surface properties are considered to be important factors in addressing proper functionalities.In this paper,a multifunctional mussel-inspired coating was prepared via the direct copolymerization of epigallocatechin gallate(EGCG)and arginine.The coating formation was confirmed by X-ray photoelectron spectroscopy and Fourier transform infrared spectra.The EGCG/arginine coating contained diverse functional groups like amines,phenols and carboxyls,whose densities were also tunable.Such mussel-inspired coating could also be applied as an ad-layer for its secondary reactivity,demonstrated by quartz crystal microbalance technique.Moreover,the tunable surface density of phenols showed potential ability in modulating endothelial cell and smooth muscle cell viability.The coatings rich in phenols presented excellent free radical scavenging property.Current results strongly indicated the potential of EGCG/arginine coatings to be applied as an ad-layer for vascular materials.展开更多
文摘Soluble receptor for advanced glycation end products(sRAGE)acts as a decoy sequestering of RAGE ligands,thus preventing the activation of the ligand-RAGE axis linking human diseases.However,the molecular mechanisms underlying sRAGE remain unclear.In this study,THP-1 monocytes were cultured in normal glucose(NG,5.5 mmol/L)and high glucose(HG,15 mmol/L)to investigate the effects of diabetesrelevant glucose concentrations on sRAGE and interleukin-1β(IL-1β)secretion.The modulatory effects of epigallocatechin gallate(EGCG)in response to HG challenge were also evaluated.HG enhanced intracellular reactive oxygen species(ROS)generation and RAGE expression.The secretion of sRAGE,including esRAGE and cRAGE,was reduced under HG conditions,together with the downregulation of a disintegrin and metallopeptidase 10(ADAM10)and nuclear factor erythroid 2-related factor 2(Nrf2)nuclear translocation.Mechanistically,the HG effects were counteracted by siRAGE and exacerbated by siNrf2.Chromatin immunoprecipitation results showed that Nrf2 binding to the ADAM10 promoter and HG interfered with this binding.Our data reinforce the notion that RAGE and Nrf2 might be sRAGE-regulating factors.Under HG conditions,the treatment of EGCG reduced ROS generation and RAGE activation.EGCG-stimulated cRAGE release was likely caused by the upregulation of the Nrf2-ADAM10 pathway.EGCG inhibited HG-mediated NLRP3 inflammasome activation at least partly by stimulating sRAGE,thereby reducing IL-1βrelease.
文摘为探究表没食子儿茶素没食子酸酯-牛骨蛋白(Epigallocatechin-3-gallate-Bovine bone protein,EGCG-BBP)对乳化肉制品蛋白结构及贮藏氧化稳定性的影响,本文研究不同EGCG-BBP添加量对生肉糜中肌原纤维蛋白(Myofibrillar protein,MP)的理化性质、结构特性以及对肉丸氧化特性的影响。结果表明:当EGCG-BBP添加量为0.8%时,肉糜中MP的巯基含量最高,达4.06 nmol/mg蛋白,且羰基含量及表面疏水性最低,能够有效提升乳化肉制品的抗氧化能力。由红外光谱分析表明,与未添加EGCG-BBP组相比,添加共价物肉糜中MP的酰胺A带峰值所对应的波数明显增大,说明MP的二级结构会随之发生改变;荧光光谱显示,随贮藏时间延长,对照组中MP的最强荧光波长发生显著红移,但随EGCG-BBP浓度的增加,红移程度显著降低,表明添加EGCGBBP能够改变MP的三级结构。此外,乳化肉丸贮藏过程中的氧化指标分析表明,添加0.8%EGCG-BBP能显著降低肉丸的过氧化值(PV)和硫代巴比妥酸值(TBARs),从而提高其氧化稳定性。综上所述,EGCG-BBP能够显著改变MP的二、三级结构,且具有良好的抗氧化性能,在提升乳化肉制品品质方面具有很大的应用潜力,为肉品抗氧化型乳化剂的应用提供新的选择。
文摘（-）-Epigallocatechin gallate （EGCG）, a major polyphenolic constituent of green tea, can inhibit activity of specific receptor tyrosine kinases （RTKs） and related downstream signal transduction pathways, resulting in the control of unwanted cell proliferation. The epidermal growth factor receptor （EGFR） signaling pathway is one of the most important pathways that regulates growth, survival,proliferation and differentiation in mammalian cells. This review addresses the effects of EGCG on some protein factors involved in the EGFR signaling pathway in a direct or indirect manner. Based on our understanding of the interaction between EGCG and these factors, and based on their structures, EGCG could be used as a lead compound for designing and synthesizing novel drugs with significant biological activity.
文摘The fresh leaves of China green tea, Camellia sinensis, were collected from Fuyang, Zhejiang Province, China, in April. The tea polyphenols was extracted by four different methods （homogenized with distilled water at room temperature, homogenized with 0.3% citric acid （w/v） at room temperature, 5- min boiling and homogenized with distilled water at room temperature, homogenized with 85℃ distilled water）, and after preserving at room temperature, the change of the Epigallocatechin gallate （EGCG） contents of the extracts was investigated. Results indicated that the EGCG content of homogenate extracted with 85℃ distilled water was the highest before the extract was preserved, followed by that of the extract homogenized with 0.3% citric acid at room temperature. During preservation, EGCG content changed obviously. The EGCG contents of homogenates extracted with distilled water at room temperature and 85℃ distilled water declined quickly and separately reduced to 21.52% and 54.6% of their initial contents after preservation for 12 h. The EGCG contents extracted by 0.3% citric acid （w/v） solvent at room temperature and 5- min boiling/homogenized with distilled water at room temperature declined relatively slowly ,and separately reduced to 76.9% and 85.16% of their initial contents after preservation for 12 h. It was also found that the citric acid can prevent the degradation of EGCG and the extract solution color is light green
文摘目的:评价表没食子儿茶素没食子酸酯(EGCG)及其甲基化修饰物(EGCG-3Me)改性粘接剂对根管牙本质粘接界面稳定性的作用。方法:将质量浓度为400μg/ml的EGCG及EGCG-3Me添加到全酸蚀粘接剂Single Bond 2(SB2)中,制备改性粘接剂E-SB2及E3-SB2,SB2为对照组。激光共聚焦显微镜和分光光度法检测改性粘接剂抗粪肠球菌的性能。微拉曼光谱仪检测粘接剂双键转化率。制备纤维桩粘接试件,用于即刻和老化后的微推出实验。结果:改性粘接剂可以抑制粪肠球菌生物膜形成,且EGCG-3Me作用更显著。改性粘接剂与SB2的双键转化率和即刻微推出粘接强度差异无显著性(P>0.05)。老化后改性粘接剂的微推出粘接强度显著高于SB2(P<0.05)。结论:EGCG和EGCG-3Me改性的粘接剂均可抑制粪肠球菌增殖并提高树脂-根管牙本质粘接界面稳定性,EGCG-3Me抗菌性能较佳。
基金The author would like to thank Dr Manfred.F.Maitz for his selfless assistance of this job and appreciate Mr Chongxi Jiang and Mrs Ru Shen for their help and contributions on sample characterization and analysis.Specially thank Miss Si Zhong and Mr Xin Wang for their help of cell compatibility evaluation.This work was supported by Natural Science Foundation of China(Grant 51173149,81330031 and 31270020)Sichuan Province Science and Technology Support Program(No.2014SZ0128)the 111 Project.The Program of Introducing Talents of Discipline to Universities(B16033).
文摘Surface properties are considered to be important factors in addressing proper functionalities.In this paper,a multifunctional mussel-inspired coating was prepared via the direct copolymerization of epigallocatechin gallate(EGCG)and arginine.The coating formation was confirmed by X-ray photoelectron spectroscopy and Fourier transform infrared spectra.The EGCG/arginine coating contained diverse functional groups like amines,phenols and carboxyls,whose densities were also tunable.Such mussel-inspired coating could also be applied as an ad-layer for its secondary reactivity,demonstrated by quartz crystal microbalance technique.Moreover,the tunable surface density of phenols showed potential ability in modulating endothelial cell and smooth muscle cell viability.The coatings rich in phenols presented excellent free radical scavenging property.Current results strongly indicated the potential of EGCG/arginine coatings to be applied as an ad-layer for vascular materials.
