Sensitivity of diagnosis of spontaneous bacterial peritonitis is higher with the automated cell count method

BACKGROUND Spontaneous bacterial peritonitis(SBP)is one of the most important complications of patients with liver cirrhosis entailing high morbidity and mortality.Making an accurate early diagnosis of this infection is key in the outcome of these patients.The current definition of SBP is based on studies performed more than 40 years ago using a manual technique to count the number of polymorphs in ascitic fluid(AF).There is a lack of data comparing the traditional cell count method with a current automated cell counter.Moreover,current international guidelines do not mention the type of cell count method to be employed and around half of the centers still rely on the traditional manual method.AIM To compare the accuracy of polymorph count on AF to diagnose SBP between the traditional manual cell count method and a modern automated cell counter against SBP cases fulfilling gold standard criteria:Positive AF culture and signs/symptoms of peritonitis.METHODS Retrospective analysis including two cohorts:Cross-sectional(cohort 1)and case-control(cohort 2),of patients with decompensated cirrhosis and ascites.Both cell count methods were conducted simultaneously.Positive SBP cases had a pathogenic bacteria isolated on AF and signs/symptoms of peritonitis.RESULTS A total of 137 cases with 5 positive-SBP,and 85 cases with 33 positive-SBP were included in cohort 1 and 2,respectively.Positive-SBP cases had worse liver function in both cohorts.The automated method showed higher sensitivity than the manual cell count:80%vs 52%,P=0.02,in cohort 2.Both methods showed very good specificity(>95%).The best cutoff using the automated cell counter was polymorph≥0.2 cells×10^(9)/L(equivalent to 200 cells/mm^(3))in AF as it has the higher sensitivity keeping a good specificity.CONCLUSION The automated cell count method should be preferred over the manual method to diagnose SBP because of its higher sensitivity.SBP definition,using the automated method,as polymorph cell count≥0.2 cells×10^(9)/L in AF would need to be considered in patients admitted with decompensated cirrhosis.

Modeling One Dimensional Two-Cell Model with Tumor Interaction Using Krylov Subspace Methods

A brain tumor occurs when abnormal cells grow, sometimes very rapidly, into an abnormal mass of tissue. The tumor can infect normal tissue, so there is an interaction between healthy and infected cell. The aim of this paper is to propose some efficient and accurate numerical methods for the computational solution of one-dimensional continuous basic models for the growth and control of brain tumors. After computing the analytical solution, we construct approximations of the solution to the problem using a standard second order finite difference method for space discretization and the Crank-Nicolson method for time discretization. Then, we investigate the convergence behavior of Conjugate gradient and generalized minimum residual as Krylov subspace methods to solve the tridiagonal toeplitz matrix system derived.

PREDICTION OF TEXTILE FABRIC REINFORCED COMPOSITE PROPERTIES BASED ON NODE INTERPOLATION CELL METHOD

Node interpolation cell method（NICM）is a micromechanics method employing the virtual displacement principle and the representative volume element（RVE）scheme to obtain the relationship between the global and the local strain.Mechanical properties of 2-D textile fabric reinforced ceramic matrix composites are predicted by NICM.Microstructures of 2-D woven and braided fabric reinforced composite are modeled by two kinds of RVE scheme.NICM is used to predict the macroscopic mechanical properties.The fill and warp yarns are simulated with cubic B-spline and their undulating forms are approximated by sinusoid.The effect of porosity on the fiber and matrix are considered as a reduction of elastic module.The connection of microstructure parameters and fiber volume fraction is modeled to investigate the reflection on the mechanical properties.The results predicted by NICM are compared with that by the finite element method（FEM）.The comparison shows that NICM is a valid and feasible method for predicting the mechanics properties of 2-D woven and braided fabric reinforced ceramic matrix composites.

Finite cell method compared to h-version finite element method for elasto-plastic problems

The finite cell method （FCM） combines the high-order finite element method （FEM） with the fictitious domain approach for the purpose of simple meshing. In the present study, the FCM is used to the Prandtl-Reuss flow theory of plasticity, and the results are compared with the h-version finite element method （h-FEM）. The numerical results show that the FCM is more efficient compared to the h-FEM for elasto-plastic problems, although the mesh does not conform to the boundary. It is also demonstrated that the FCM performs well for elasto-plastic loading and unloading.

Shape optimization of axisymmetric solids with the finite cell method using a fixed grid

In this work, a design procedure extending the B-spline based finite cell method into shape optimization is developed for axisymmetric solids involving the centrifugal force effect. We first replace the traditional conforming mesh in the finite element method with structured cells that are fixed during the whole design process with a view to avoid the sophisticated re-meshing and eventual mesh distortion.Then, B-spline shape functions are further implemented to yield a high-order continuity field along the cell boundary in stress analysis. By means of the implicit description of the shape boundary, stress sensitivity is analytically derived with respect to shape design variables. Finally, we illustrate the efficiency and accuracy of the proposed protocol by several numerical test cases as well as a whole design procedure carried out on an aeronautic turbine disk.

Research method of psoriasis based on HaCaT cells

Psoriasis is a chronic,refractory,inflammatory skin disease that occurs in young adults.The traditional animal model cannot simulate the skin characteristics of patients with psoriasis effectively,so it is difficult to be used for in-depth study of psoriasis mechanism.Immortalized human epidermal cells(HaCaT) is a non-tumor,immortalized human epidermal cell which is widely used in the study of dermatosis.HaCaT cells are the best choice for the study of psoriasis mechanism because their immu.nological characteristics and reproductive ability are coincide with the pathological features of psoriasis.This article reviews the specific methods such as establishment of cell method,cytokine and chemo.kine analysisin the pathogenesis study of psoriasis based on HaCaT cells,hoping to provide some thoughts for drug′s pharmacological activity research.

ONE-CELL-STATE METHOD FOR DETERMINATION OF ELECTRONIC STRUCTURE OF INTERMETALLIC COMPOUNDS

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Establishment and validation of a method for cell irradiation in 96-well and 6-well plates using a linear accelerator

To establish and validate a method for cell irradiation in 96-well and 6-well plates using a linear accelerator, three irradiation methods(G0 B0 F40,G0 B1.5 F40, and G180 B1.5 F40) were designed to irradiate cell culture plasticware simulated with RW3 slab phantom and polystyrene. The difference between the actual physical measured dose and the preset dose was compared among the three methods under the preparatory conditions of 2, 4, 6, 8, and 10 Gy. MDA-MB-231 cells were analyzed by using a cell proliferation assay and a clonogenic assay to verify the difference between the three cell irradiation methods on cell radiosensitivity. For each preset dose, the difference between the actual measured dose and the preset dose was the lowest for Method G0 B1.5 F40, the second lowest for Method G180 B1.5 F40, and the maximum for Method GOB0 F40. The ranges of the differences were-0.28 to 0.02%,-2.17 to-1.80%, and-4.92 to-4.55%, and 0.31 to-0.12%,-3.42 to-2.86%, and-7.31 to-6.92%,respectively, for 96-well and 6-well plates. The cell culture experiments proved that Method G0 B1.5 F40 was an accurate, effective, simple, and practical irradiation method. The most accurate and effective cell irradiation method should always be used, as it will reduce dose differences and instability factors and provide improved accuracy and comparability for laboratories researching cellular radiosensitivity.

Stochastic response analysis of noisy system with non-negative real-power restoring force by generalized cell mapping method

The stochastic response of a noisy system with non-negative restoring force is investigated. The generalized cell mapping （GCM） method compute the transient and stationary probability density functions （PDFs） real-power is used to Combined with the global properties of the noise-free system, the evolutionary process of the tran- sient PDFs is revealed. The results show that stochastic P-bifurcation occurs when the system parameter varies in the response analysis and the stationary PDF evolves from bimodal to unimodal along the unstable manifold during the bifurcation.

A porous cell method for the simulation of fluid-solid interactions

There are many ways of describing a solid,porous or fluid region of the computational domain when solving the Navier-Stokes equations(NSE)for flow motions.Amongst these the porous cell method is one of the most flexible approaches.In this method,a parameter is defined as a ratio of the volume open to water and air in a calculation cell to its cell volume.In the calculation,the same numerical procedure is applied to every cell and no explicit boundary conditions are needed at solid boundaries.The method is used to simulate flow through porous media,around solid bodies and over a moving seabed.The results compare well with experimental data and other numerical results.In our future work the porous cell method will be applied to more complex fluid-solid interaction situations.

Preparation of Se-based solar cell using spin-coating method in ambient condition

A new hybrid organic-inorganic structure of FTO/TiO2/Se/HTL/Au based selenium solar cell has been fabricated through a low-cost spin-coating process in air. In this process, selenium is completely dissolved in hydrazine, to fk）rm a homogeneous precursor solution. After spin-coating the precursor solution on the TiO2 substrates, following by sintering at 200 ℃ for 5rain, a uniform selenium film with crystalline grains is formed. The selenium based solar cell exhibits an efficiency of 1.23% under AM1.5 illumination （100 mW.cm-2）, short-circuit current density of 8 mA.cm 2, open-circuit voltage of 0.55 V, and fill factor of 0.37. Moreover, the device shows a stable ability with almost the same performance alter 60 days.

Direct Numerical Simulation of a Jet Issuing from Rectangular Nozzle by the Vortex in Cell Method

Direct numerical simulation of a jet issuing from a nozzle having a rectangular cross-section is conducted. The vortex in cell (VIC) method, of which computational accuracy was heightened by the authors in a prior study, is used for the DNS. The aspect ratio of the nozzle cross-section is 15, and the Reynolds number based on the shorter side length of the nozzle exit is 6700. The turbulence statics, such as the mean velocity and the turbulence intensity, are favorably compared with the experimentally measured results. The behavior of the large-scale eddies as well as the development of the turbulent flow is also confirmed to agree with the measurement. These indicate that the authors’ VIC method is successfully employed for the DNS of rectangular jet.

Spherical silicon solar cell with reflector cup fabricated by decompression dropping method

A spherical Si solar cell with a reflector cup was successfully fabricated by a dropping method at decompression state. In the dropping method, melted Si droplets were instilled at decompression state (0.5×105 Pa) to reduce crystal growth rate, dominating crystal quality such as dislocation density in crystal grains. Spherical Si solar cells were fabricated using the spherical Si crystals with a diameter of 1 mm and then mounted on a reflector cup. The current-voltage measurement of the solar cell shows an energy conversion efficiency of 11.1% (short-circuit current density (Jsc): 24.7 mA·cm-2, open-circuit voltage: 601 mV, fill factor: 74.6%). Minority carrier diffusion length determined by surface photovoltage method was 98 μm. This value can be enhanced by the improvement of crystal quality of spherical Si crystals. These results demonstrate that spherical Si crystals fabricated by the dropping method has a great potential for substrate material of high-efficiency and low-cost solar cells.

Study of a Quantitative Fluorescence Method for Interleukin 2 Receptor of Mononuclear Cell in Normal Human Peripheral Blood

A quantitative method using fluorescence spectro-photometer to detect theinterleukin 2 reocptor （IL 2R） on the surface of mononuclear cells in normal humanperipheral blood is described. For expression of IL 2R, the optimum conoentration of PHA andCon A were 100 μg/ml and 10 μg/ml respectively. The PHA induced effect on mononuclear cellswas better than Con A induced one. The peak value of IL 2R cxpression was at the 24th h afterstimulation The optimum dilution of the first antibody （anti-Tac） was 1: 50, while the dilution ofthe second antibody（fluorescein conjugated rabbit anti-mousc IgG） was 1: 32 Their optimumincubation period was 20 and 10 min repectively. The concentration of fluorcscein-conjugatedantibody per 1×106 mononuclear cells from the same normal human peripheral blood was5.22±0.45×10-10 mol, and the coefficient of variation was 8. 6%. The concentration offluorescein-conjugated antibody per 1×106PBMC from five healthy individuals was 4. 8±0. 97×10-10 mol Therefore this assay system is stable and quite reproducible.

A NEW METHOD OF ISOLATING KUPFFER CELLS FROM BIOPSY TISSUE OF RAT LIVER

The isolation of a high yield and purity of Kupffer cells has been reported in detail.1 This paper reports into the research about isolation Kupffer cells from biopsy tissue of liver. This method includes 5 important steps: (1) take fresh liver tissue, and mince with scissors. (2) spin at low speed to wash off red blood cells. (3) digest in collagenase for suitable time. (4) isolate Kupffer cells on a percoll density gradient. (5) cell charaterization was observed by N.S.E stain and peroxidatic activity with lumino-meter measurement and phagocytosis with latex beads.2.3

Synthesis of the CuInSe_2 thin film for solar cells using the electrodeposition technique and Taguchi method

The Taguchi method was used to obtain the optimum electrodeposition parameters for the synthesis of the CuInSe2 thin film for solar cells. The parameters consist of annealing temperature, current density, CuCl2 concentration, FeCl3 concentration, H2SeO3 concentration, TEA amount, pH value, and deposition time. The experiments were carried out according to an L18（2^13^7） table An X-ray diffractometer （XRD） and a scanning electron microscope （SEM） were respectively used to analyze the phases and observe the microstructure and the grain size of the CuInSe2 film before and after annealing treatment. The results showed that the CuInSe2 phase was deposited with a preferred plane （112） parallel to the substrate surface. The optimum parameters are as follows： current density, 7 mA/cm^2; CuCl2 concentration, 10 mM; FeCl3 concentration, 50 mM; H2SeO3 concentration, 15 mM; TEA amount, 0 mL; pH value, 1.65; deposition time, 10 min; and annealing temperature, 500℃.

In vitro cultivation of rat bone marrow mesenchymal stem cells and establishment of pEGFP/Ang-1 transfection method

Objective:To obtain the bone marrow mesenchymal stem cells(BMSCs).complete phenotypic identification and successfully transfecl rat BMSCs by recombinant plasmid pF.GFP/Ang-1.Methods:BMSCs were isolated from bone marrow using density gradient centrifugation method and adherence screening method,and purified.Then the recombinant plasmid pEGFP/Ang-1was used to transfect BMSCs and the positive clones were obtained by the screen of C418 and observed under light microscopy inversely.Green fluorescent exhibited by protein was enhanced to measure the change time of the expression amount of Ang-1.Results:BMSCs cell lines were obtained successfully by adherence screening method and density gradient ccntrifugation.Ang-1 recombinant plasmid was transfected smoothly into rat BMSCs,which can express Ang-1 for 3 d and decreased after 7 d.Conclusions:Adherence screening method und density gradient ceiilrifugation can be effective methods lo obtain BMSCs with high purity and rapid proliferation.Besides,the expression of transfected recombinant plasmid pEGFP/Ang-1 in rat BMSCs is satisfactory.

基于直觉模糊n-cell数的多属性决策方法及应用

针对权重已知的直觉模糊多属性决策问题,提出了一种基于直觉模糊n-cell数的决策方法。定义了直觉模糊n-cell数的偏序和两种基于均值和离散度的弱序。研究了所提出序的理论性质,并进行比较分析。最后,将相关排序方法应用于一类人才选拔的问题之中,在解决问题的同时验证了方法的有效性。