AIM:To determine the expression and significance of filamin A(FLNa)in colorectal adenocarcinoma tissue.METHODS:The expression of FLNa in 46 colorectal cancer tissues and normal tissues was detected by immunohistochemi...AIM:To determine the expression and significance of filamin A(FLNa)in colorectal adenocarcinoma tissue.METHODS:The expression of FLNa in 46 colorectal cancer tissues and normal tissues was detected by immunohistochemistry,reverse transcription polymerase chain reaction(RT-PCR)and Western blotting,and its relationship with clinical parameters and prognosis was analyzed.RESULTS:The positive expression of FLNa in cancer tissues was lower than that in normal mucosa,and the difference was statistically significant.The expression of FLNa correlated with liver metastasis,lymph node metastasis and rectal invasion depth,regardless of sex,age,tumor location,tumor size,gross shape and histological type of colorectal carcinoma.Multivariate analysis showed that FLNa was an independent risk factor for postoperative survival of patients with colorectal adenocarcinoma.Moreover,survival analysis showed that the expression level of FLNa was closely related with survival of patients with colorectal adenocarcinoma.The results of RT-PCR and Western blotting were consistent with those of immunohistochemistry.CONCLUSION:FLNa showed low expression in colorectal adenocarcinoma,high correlation with the incidence and development of colorectal cancer,and was considered an indicator of prognosis.展开更多
目的:研究鼻及鼻窦黏膜黑色素瘤(sinus and nasal mucosal melanoma,SNMM)Ⅲ-Ⅳa期[2010年美国AJCC分期(第7次修订版)]的临床及组织病理学特点、手术治疗及辅助治疗对预后的影响因素。方法:本文对2006年至2017年间收治的满足Ⅲ-Ⅳa期鼻...目的:研究鼻及鼻窦黏膜黑色素瘤(sinus and nasal mucosal melanoma,SNMM)Ⅲ-Ⅳa期[2010年美国AJCC分期(第7次修订版)]的临床及组织病理学特点、手术治疗及辅助治疗对预后的影响因素。方法:本文对2006年至2017年间收治的满足Ⅲ-Ⅳa期鼻及鼻窦黏膜黑色素瘤患者临床资料进行回顾性研究。结果:最终共纳入16例满足条件的鼻及鼻窦黏膜黑色素瘤患者,其中7例男性,9例女性,平均年龄为63.5(35~81)岁,随访时间为23.5(7~76)个月。患者1年生存率为81.25%,3年生存率为18.75%,5年生存率为12.50%。接受术后辅助治疗患者11例,其中,术后辅助放疗5例,术后辅助生物治疗6例。结论:鼻及鼻窦黏膜黑色素瘤恶性度高,预后差,术后复发和转移发生率较高,彻底清除肿瘤病灶的手术联合术后辅助治疗的综合治疗为首选治疗方法。展开更多
AIM: To investigate member 3a of Wingless-type MMTV integration site family (Wnt3a) expression in cancerous and surrounding tissues and the relationship between clinicopathologic features of hepatocellular carcinoma (...AIM: To investigate member 3a of Wingless-type MMTV integration site family (Wnt3a) expression in cancerous and surrounding tissues and the relationship between clinicopathologic features of hepatocellular carcinoma (HCC) and Wnt3a expression.METHODS: Wnt3a expression and cellular distribution and clinicopathologic characteristics in cancerous tissue and matched surrounding tissues were analyzed in 80 HCC patients from January 2006 to August 2008 by tissue microarrays and immunohistochemistry. The overall and disease-free survival rates were estimated using the Kaplan-Meier method and compared with the log-rank test. The prognostic analysis was carried out with univariate and multivariate Cox regressions models.RESULTS: The incidence of oncogenic Wnt3a expression in the cancerous group was up to 96.25% (77 of 80), which was significantly higher (χ<sup>2</sup> = 48.818, P < 0.001) than that in the surrounding group (46.25%, 37 of 80). Brown Wnt3a staining gradually increased with clinical staging that showed very strong staining in advanced HCC. The clinicopathologic features of high Wnt3a expression in HCC were related to poorly-differentiated grade (χ<sup>2</sup> = 20.211, P < 0.001), liver cirrhosis (χ<sup>2</sup> = 8.467, P < 0.004), hepatitis B virus (HBV) infection (χ<sup>2</sup> = 12.957, P < 0.001), higher tumor-node-metastasis stage (χ<sup>2</sup> = 22.960, P < 0.001), and 5-year survival rate (χ<sup>2</sup> = 15.469, P < 0.001).CONCLUSION: Oncogenic Wnt3a expression associated with HBV infection and cirrhotic liver might be an independent prognostic factor for HCC.展开更多
基金Supported by Hebei Province Health Department of China,No.14277710D and No.GL2012050
文摘AIM:To determine the expression and significance of filamin A(FLNa)in colorectal adenocarcinoma tissue.METHODS:The expression of FLNa in 46 colorectal cancer tissues and normal tissues was detected by immunohistochemistry,reverse transcription polymerase chain reaction(RT-PCR)and Western blotting,and its relationship with clinical parameters and prognosis was analyzed.RESULTS:The positive expression of FLNa in cancer tissues was lower than that in normal mucosa,and the difference was statistically significant.The expression of FLNa correlated with liver metastasis,lymph node metastasis and rectal invasion depth,regardless of sex,age,tumor location,tumor size,gross shape and histological type of colorectal carcinoma.Multivariate analysis showed that FLNa was an independent risk factor for postoperative survival of patients with colorectal adenocarcinoma.Moreover,survival analysis showed that the expression level of FLNa was closely related with survival of patients with colorectal adenocarcinoma.The results of RT-PCR and Western blotting were consistent with those of immunohistochemistry.CONCLUSION:FLNa showed low expression in colorectal adenocarcinoma,high correlation with the incidence and development of colorectal cancer,and was considered an indicator of prognosis.
文摘目的:研究鼻及鼻窦黏膜黑色素瘤(sinus and nasal mucosal melanoma,SNMM)Ⅲ-Ⅳa期[2010年美国AJCC分期(第7次修订版)]的临床及组织病理学特点、手术治疗及辅助治疗对预后的影响因素。方法:本文对2006年至2017年间收治的满足Ⅲ-Ⅳa期鼻及鼻窦黏膜黑色素瘤患者临床资料进行回顾性研究。结果:最终共纳入16例满足条件的鼻及鼻窦黏膜黑色素瘤患者,其中7例男性,9例女性,平均年龄为63.5(35~81)岁,随访时间为23.5(7~76)个月。患者1年生存率为81.25%,3年生存率为18.75%,5年生存率为12.50%。接受术后辅助治疗患者11例,其中,术后辅助放疗5例,术后辅助生物治疗6例。结论:鼻及鼻窦黏膜黑色素瘤恶性度高,预后差,术后复发和转移发生率较高,彻底清除肿瘤病灶的手术联合术后辅助治疗的综合治疗为首选治疗方法。
基金Supported by the International S&T Cooperation Program,No.2013DFA32150 of China
文摘AIM: To investigate member 3a of Wingless-type MMTV integration site family (Wnt3a) expression in cancerous and surrounding tissues and the relationship between clinicopathologic features of hepatocellular carcinoma (HCC) and Wnt3a expression.METHODS: Wnt3a expression and cellular distribution and clinicopathologic characteristics in cancerous tissue and matched surrounding tissues were analyzed in 80 HCC patients from January 2006 to August 2008 by tissue microarrays and immunohistochemistry. The overall and disease-free survival rates were estimated using the Kaplan-Meier method and compared with the log-rank test. The prognostic analysis was carried out with univariate and multivariate Cox regressions models.RESULTS: The incidence of oncogenic Wnt3a expression in the cancerous group was up to 96.25% (77 of 80), which was significantly higher (χ<sup>2</sup> = 48.818, P < 0.001) than that in the surrounding group (46.25%, 37 of 80). Brown Wnt3a staining gradually increased with clinical staging that showed very strong staining in advanced HCC. The clinicopathologic features of high Wnt3a expression in HCC were related to poorly-differentiated grade (χ<sup>2</sup> = 20.211, P < 0.001), liver cirrhosis (χ<sup>2</sup> = 8.467, P < 0.004), hepatitis B virus (HBV) infection (χ<sup>2</sup> = 12.957, P < 0.001), higher tumor-node-metastasis stage (χ<sup>2</sup> = 22.960, P < 0.001), and 5-year survival rate (χ<sup>2</sup> = 15.469, P < 0.001).CONCLUSION: Oncogenic Wnt3a expression associated with HBV infection and cirrhotic liver might be an independent prognostic factor for HCC.
