Study on Biological Characters of Asparagus macowanii Baker.

Asparagus macowanfi Baker, is a climbing herbaceous foliage species in genus Asparagus of Liliaceae, This paper summarized its multiple uses, morphologi- cal characteristics, biological habit, reproduction methods, management after cultiva- tion, prevention and control of pests and disease, as well as harvest and grading, with the objective to provide references for the exploitation and utilization of As- paragus macowanii Baker.

AN OBSERVATION STUDY ON BIOLOGICAL CHARACTERS AND MATING BEHAVIOR OF DAHURIAN CASEBEARER(COLEOPHORA DAHURICA FLKV.)ADULTS

Through rearing tests and observations under laboratory conditions, biological characters and mating behavior of Dahurian casebearer (Coleophora dahurica Flkv.) adults were studied in this paper. Results discovered that the time of emergense of male adults was 24h to 36h earlier than that of female adults. The peak of sex pheromone secretion of the female adults appeared of 24h to 42h after the emergence. The reaction of male adults on the sex pheromone was most sensitive during 30h to 48h after emerged, so the earlier emergence of the male adults was favorable to mating. The phenomenon of repeated mating did not exist in female adults, but the male adults could mate for several times, with maximum of 4 times. There was a definite behavioral reaction during the mating period of male and female adults, and the certain period of feeding and mating times could affect the adults longervity to a certain degree.

Study on biological characters of SGC7901 gastric cancer cell-dendritic cell fusion vaccines

AIM： To detect the biological characters of the SGC7901 gastric cancer cell-dendritic cell fusion vaccines.METHODS： The suspending living SGC7901 gastric cancer cells and dendritic cells were induced to be fusioned by polyethylene glycol. Pure fusion cells were obtained by selective culture with the HAT/HT culture systems. The fusion cells were counted at different time points of culture and their growth curves were drawn to reflect their proliferative activities. The fusion cells were also cultured in culture medium to investigate whether they could grow into cell clones. MTT method was used to test the stimulating abilities of the fusion cells on T lymphocytes＇ proliferations. Moreover, the fusion cells were planted into nude mice to observe whether they could grow into new planted tumors in this kind of immunodeficiency animals.RESULTS： The fusion cells had weaker proliferative activity and clone abilities than their parental cells. When they were cultured, the counts of cells did not increase remarkably, nor could they grow into cell clones in culture medium. The fusion cells could not grow into new planted tumors after planted into nude mice. The stimulating abilities of the fusion cells on T lymphocytes＇ proliferations were remarkably increased than their parental dendritic cells. CONCLUSION： The SGC7901 gastric cancer cell-dendritic cell fusion vaccines have much weaker proliferative abilities than their parental cells, but they keep strong abilities to irritate the T lymphocytes and have no abilities to grow into new planted tumors in immunodeficiency animals. These are the biological basis for their antitumor biotherapies.

Comparison of biological characteristics of marrow mesenchymal stem cells in hepatitis B patients and normal adults

AIM: To establish a culture system of marrow mesenchymal stem cells (MSCs)from hepatitis B patients and normal adults and to compare their biological characteristics. METHODS: MSCs were isolated from bone marrow in 34 male hepatitis B patients and 15 male normal adults and cultivated in vitro. Their biological characteristics including surface markers, shapes and appearances, growth curves, first passage time and passage generations were compared. RESULTS: Cultivation achievement ratio of hepatitis B patients was lower than that of normal adults, no statistical significance (82.35% vs 100%, P > 0.05). Compared with MSCs of normal adults, MSCs of hepatitis B patients presented a statistical lower growth curve, longer first passage time (13.0 ± 1.6 d vs 11.4 ± 1.5 d, P < 0.05), fewer passaging generation numbers (10.5 ± 1.4 generations vs 12.3 ± 1.7 generations, P < 0.05), though both shared same appearances, shapes and surface markers. MSCs in hepatitis B patients would expand, spread out and age more easily and there were more refractive particles in the cytoplasm. CONCLUSION: MSCs from hepatitis B patients can be cultured in vitro. Although their appearance, shape and surface marker are similar to those of MSCs from normal adults, there are differences in their biological characteristics.

Observation of Biological Character of Drosophila melanogaster Meigen on Cherry and Screening of Its Insecticides

Indoor rearing observation and efficacy test of insecticides were conducted in 2010 to understand the biochemical characteristics of Drosophila rnelano-gaster and high efficient insecticides against the insect pest. The results showed that D. melanogaster had preference to eight kinds of fruits with proliferation. Fe-male adults of D. melanogaster laid eggs under epidermis of cherry fruits and newly hatched larvae caused damage on fruits. When the temperatures were （ 18 ±2） ℃ and （ 23 ± 2 ） ℃, the egg stage was 2.4 - 3.0 and 1.2 - 1.8 d ; the larval stage was 11.2 - 12.2 and 5.6 - 6.5 d; the papal stage was 9.5 - 10.6 and 5.0 - 6.2 d ; one generation was 23.1 - 25.4 and 12.8 - 14.2 d, respectively. Four insecticides （ including 1% emamectin benzoate EC 3 000 times dilution, 90% crys- tal trichlorfon 1 000 times dilution, 40% phoxim EC 1 500 times dilution and 25% buprofezin WP 2 000 times dilution） had higher control effects against D. mela-nogaster, with mortalities of 85.00% - 100.00%, 96.67% - 100.00% and 100.00% after insecticide application for 1, 3 and 6 h, respectively.

Review on studies of Eupatorium adenophorum--an important invasive species in China

Eupatorium adenophorum Spreng. was introduced in Yunnan Province of China around 1940. Since then it has been spreading rapidly, particularly in the southern and southwestern parts of China and caused serious economic loss. The biological research and inte-grated control on E. adenophorum were carried out from 1980's in Yunnan Province. Together with other 15 invasive external species, the weed has been listed in the White Paper by The State Environmental Protection Administration of China. This paper briefly reviews the studies on natural distribution, biological character, ecological character, chemical component, hazard, potential application and the control of E. adenophorum. The research direction for this invasive external species in future was also discussed.

Biological character of human adipose-derived adult stem cells and influence of donor age on cell replication in culture

To investigate the biological character of human adipose-derived adult stem cells (hADAS cells) when cultured in vitro and the relationship between hADAS cell’s replication activity and the donor’s age factor, and to assess the stem cells as a new source for tissue engineering. hADAS cells are isolated from human adipose tissue of different age groups (from adolescents to olds: <20 years old, 21―40 years old, 41―60 years old and >61 years old groups). The protein markers (CD29, CD34, CD44, CD45, CD49d, HLA-DR, CD106) of hADAS cells were detected by flow cytometry (FCM) to identify the stem cell, and the cell cycle was examined for P20 hADAS cells to evaluate the safety of the subculture in vitro. The generative activity of hADAS cells in different age groups was also examined by MTT method. The formula “ log2T D = t logN t ? logN 0” was used to get the time doubling (TD) of the cells. The results showed that the cells kept heredity stabilization by chromosome analysis for at least 20 passages. The TD of these cells increased progressively by ageing, and the TD of the <20 years old group was lower than that of the >61 years old group (statistical analysis of variance (ANOVA), P=0.002, P<0.05). These find- ings suggested that a higher level of hADAS cells replication activity was found in the younger dona- tors, and they represent novel and valuable seed cells for studies of tissue engineering.

The Temporal-Spatial Distribution of Light Intensity in Maize and Soybean Intercropping Systems

Intercropping can improve field microclimates, decrease the incidence of crop diseases, and increase crop yields, but the reasons for this remain unknown. Solar radiation is the most important environmental influence. To understand the mechanisms of intercropping we established an experiment consisting of three cropping patterns： a monocropping control {treatment A） and two intercropping treatments （B： two rows of maize and two rows of soybean intercropping; C： two rows of maize and four rows of soybean intercropping）. Results show that compared to monocropping, intercropping increased the amount of light penetrating to inferior leaves in maize plants. Light intensity reaching maize plants at the heading stage in intercropping increased over two-fold at 30 cm above ground and 10-fold at 70 cm above ground, compared with monocropping. At the flowering to maturity stage, light intensity at 110, 160 and 210 cm above ground among maize plants was greatly increased in intercropping compared with monocropping, by some five-fold, two-fold and 12%, respectively. Moreover, light intensity declined more slowly at the measured heights in the intercropping system compared with monocropping. From the 7-18th leaf, light intensity per leaf increased two-fold in intercropping compared with monocropping. Daily light duration increased more than a mean of 5 h per day per leaf in intercropping compared with monocropping. The biological characters of maize including thousand kernel weight, yield per plant and area of ear leaves were all greater in intercropping than monocropping. These results suggest that, for maize, intercropping improves light density and duration significantly and this may contribute to biomass and yield increases.