Elicitation on Artemisinin Biosynthesis in Artemisia annua Hairy Roots by the Oligosaccharide Extract from the Endophytic Colletotrichum sp. B501

The oligosaccharide elicitor from the mycelial wall of an endophytic Colletotrichum sp. B501 promoted the production of artemisinin in Artemisia annua L. hairy root culture. When hairy roots of 22-day-old cultures (later growth phase) were exposed to the elicitor (20 mg/L) for 4 d, the maximum content of artemisinin reached 1.15 mg/g, a 64.29% increment over the control. The electron X-ray microanalysis disclosed the rapid accumulation of Ca 2+ in the elicited cortical cells of hairy root. The electronic microscope observation revealed the high electron density area in vacuole of elicited cells. During the first day of elicitation the peroxidase activity of hairy roots was improved sharply. Some cellular morphological changes including cell shrinkage, condensation of cytoplasm and nuclear fragmentation, coincident with the appearance of DNA ladders, were observed after the third day of elicitation. It was suggested that the oligosaccharide elicitor triggered the programmed cell death, which may provide the substance or chemical signal for artemisinin biosynthesis.

Economical Requirements Elicitation Techniques During COVID-19: A Systematic Literature Review

Requirements elicitation is a fundamental phase of software development in which an analyst discovers the needs of different stakeholders and transforms them into requirements.This phase is cost-and time-intensive,and a project may fail if there are excessive costs and schedule overruns.COVID-19 has affected the software industry by reducing interactions between developers and customers.Such a lack of interaction is a key reason for the failure of software projects.Projects can also fail when customers do not know precisely what they want.Furthermore,selecting the unsuitable elicitation technique can also cause project failure.The present study,therefore,aimed to identify which requirements elicitation technique is the most cost-effective for large-scale projects when time to market is a critical issue or when the customer is not available.To that end,we conducted a systematic literature review on requirements elicitation techniques.Most primary studies identified introspection as the best technique,followed by survey and brainstorming.This finding suggests that introspection should be the first choice of elicitation technique,especially when the customer is not available or the project has strict time and cost constraints.Moreover,introspection should also be used as the starting point in the elicitation process of a large-scale project,and all known requirements should be elicited using this technique.

Evaluating Biotic Elicitation with Phenylalanine and/or Yeast for Rosemary (<i>Rosmarinus officinals</i>L.) Sustainable Improvement under Traditional and Organic Agriculture

Rosemary (Rosmrinus officinals) is one of the most important medicinal plants was cultivated for two subsequent seasons field experiment trial (March 2018, 2019) designed as factorial split-plot design with three replicates. The main factor 4 biotic elicitors: control (E1), phenylalanine (E2), yeast (E3) and (E4), E2 + F3 Whereas, the sub-main factor, four fertilizers: (F1) NPK, (F2) 1/2NPK + PGPB and humic acid + PGPB (F3), and moringa dry leaves extract. + PGPB (F4). Statistical analysis for collected data revealed significant promotion for growth traits leading to significant increment biomass yield, secondary metabolites production and quality. Total phenolics, total flavonoids and essential oil its terpenes contents in which solitary, E2, E3 acted positive significant impact while E4exhiboted significant positive impact over E1 whereas, F1-4 achieved significant increment in which, biofertilser f4 > f3 > f2 over NPK biofertilser, while paired E1-4 with F1-4 performed E4E4 exceeded E3F3 exceeded E3F2 that exceeded E1F1. Therefore, multi-repeating elicitation with E2, 3, 4 coupled with Biofertilizers F2, 4 could be considered as eco-friendly innovative reliable practical application for sustainable improvement and sustainable use that exceeded significantly over traditional agriculture NPK alone or 1/2 NPK-PGPB for R. officinals.

An Investigation of Confucian Heuristic Teaching Elicitation Teaching Theory Theory and Socrates

Confucianism （especially its teaching theory） is the core of Chinese traditional culture with Confucius as its representative. It has played an indispensable role in the development of Chinese culture. It is the spiritual pillar of Chinese sustainable development. There are no others that can compare with its teaching method and art. The famous ＂heuristic teaching theory＂ is one of its greatest inventions. But many people think that it is Socrates invention. In fact, Socrates ＂elicitation teaching theory＂ was invented far later than that of Confucius. This paper aims at investigating of these two theories.

Probability Elicitation in Influence Diagram Modeling by Using Interval Probability

In decision modeling with influence diagrams, the most challenging task is probability elicitation from domain experts. It is usually very difficult for experts to directly assign precise probabilities to chance nodes. In this paper, we propose an approach to elicit probability effectively by using the concept of interval probability (IP). During the elicitation process, a group of experts assign intervals to probabilities instead of assigning exact values. Then the intervals are combined and converted into the point valued probabilities. The detailed steps of the elicitation process are given and illustrated by constructing the influence diagram for employee recruitment decision for a China’s IT Company. The proposed approach provides a convenient and comfortable way for experts to assess probabilities. It is useful in influence diagrams modeling as well as in other subjective probability elicitation situations.

A Discussion of Usefulness and Pitfall of Elicitation and Introspection

Nowadays, many scholars would like to use evidence derived from elicitation and introspection in their research. Both elicitation and introspection are common methods of data collection. The essay discusses the usefulness and pitfall of elicitation and introspection. It finds out that both elicitation and introspection have their own advantages and disadvantages.

Local Shear Stresses Elicit Different Mechanical Response and Gene Expression from Complex Stresses

It is well established that living cells and tissues respond to mechanical forces such as flow-related shear stresses in blood or interstitial space and complex tractional stresses at cell-matrix contacts and cell-cell contacts.However,how different modes of forces impact mechanical and biological responses is elusive.Here we describe a strategy of using the three-dimensional magnetic twisting cytometry(3D MTC)technology to apply forces in any desired directions to the same living cell.We reveal that for a fixed stress amplitude and frequency,a live cell exhibits mechanical anisotropy and responds to a local shear stress differently from responding to a local complex stress by stretching chromatin and upregulating gene transcription to different levels,extending our previous finding on force-induced direct gene activation.This finding highlights the importance of force modes in impacting cellular mechanical and biological responses in living cells and tissues and may have implications in tissue patterning and embryonic development.

Eliciting Requirements from Stakeholders’ Responses Using Natural Language Processing

Most software systems have different stakeholders with a variety of concerns.The process of collecting requirements from a large number of stakeholders is vital but challenging.We propose an efficient,automatic approach to collecting requirements from different stakeholders’responses to a specific question.We use natural language processing techniques to get the stakeholder response that represents most other stakeholders’responses.This study improves existing practices in three ways:Firstly,it reduces the human effort needed to collect the requirements;secondly,it reduces the time required to carry out this task with a large number of stakeholders;thirdly,it underlines the importance of using of data mining techniques in various software engineering steps.Our approach uses tokenization,stop word removal,and word lemmatization to create a list of frequently accruing words.It then creates a similarity matrix to calculate the score value for each response and selects the answer with the highest score.Our experiments show that using this approach significantly reduces the time and effort needed to collect requirements and does so with a sufficient degree of accuracy.

Hermeneutical Elicitation of Requirements: A Technical Perspective to Improve the Conception of the Software Requirements

In order to develop quality software that meets the originals needs of its users, it is necessary to perform the Requirements Engineering, so that the software context to be developed is identified, examined and specified properly. However, there is a problem that is increasingly in debate: the difficulty in understanding and establishing the purpose of the software to be developed, as pointed out by important researches in the area, such as the Chaos Report, which indicates that only 29% of software projects are successful, and the Software Engineering Institute, which points out software requirements as a critical factor for the success of software engineering and that deficiencies in this dimension are the main causes of software project failures. This article presents a proposal to address this problem through the use of the Hermeneutical Elicitation of Requirements, which is the conceptual adequacy of some hermeneutical methods in a technical approach that assists the requirements engineer to conceive better of the software requirements. In this way, the software engineer will be better able to develop the software to better meet the needs of its end users and sponsors.

Exogenous Pre-Harvest Treatment with Methyl Jasmonate and Chitosan Elicits Lycopene Biosynthesis in Tomato Plants

In this study, exogenous pre-harvest treatment of tomato plants with MeJA （Methyl Jasmonate） and chitosan was used to elicit lycopene biosynthesis and stimulate plant growth. Lycopene is a bright red carotene and carotenoid pigment found in tomatoes, carrots and other red fruits and vegetables. It is a powerful antioxidant that protects cells from damage and blocks cancerous cell growth. Many studies suggest eating lycopene-rich foods may be directly linked to reducing risk of several cancers, heart disease and stroke, hence there is a need to enhance its levels in tomatoes. Tomato seeds were soaked in MeJA and chitosan solutions of varying concentrations and then planted. The resulting plants were irrigated with the solutions used to soak the seeds, accordingly. The heights of the plants were recorded on a tri-weekly basis and HPLC （High Performance Liquid Chromatography） was used to analyze the effects of the MeJA and chitosan solutions on lycopene yields in the tomato fruits. Analysis of chromatograms obtained from tomato samples in the MeJA and chitosan-treated groups showed enhanced levels of lycopene. Plant growth was not significantly affected by treatment ofMeJA. Chitosan, however, noticeably increased plant growth over 7 weeks.

Discovery of New Stilbene Antioxidants of the Bio-Elicited Peanut Sprout Powder (BPSP) and Longevity Extension of Mice Fed with BPSP-Supplemented Diets

Biosynthesis of peanut stilbenes, including resveratrol as the secondary metabolites, could be enhanced by subjecting the kernels to germination and wound-stress. Investigations of the bio-elicited peanut sprout powder (BPSP) addressed on characterization of the comprising stilbenes and effectiveness in longevity extension deserves intensive research. In this study, peanut kernels were subjected to germination and wound-stress in preparation of BPSP. The methanol extracts of BPSP were medium pressure liquid chromatographic (MPLC) fractionated and semi-preparative HPLC recovered and followed by instrumental identification and biological activity determinations of the isolated stilbenes. In longevity experiments, 16 female 11-mon-old BALB/c mice and both genders of 12-mon-old ICR mice were daily fed with BPSP supplemented diets at doses of 0, 0.1 and 0.5 g BPSP/kg bw for 750 and 762 days, respectively. Based on chemical characterization, enriched quantity of stilbenes in the BPSP up to ca. 1% (w/w) was detected. Two new stilbene compounds, namely, 4, 5’-dihydroxy-6’’-hydroxymethyl, 6’’-methylpyrano [2’’, 3’’: 3’, 4’] stilbene and 3, 4, 5’-trihydroxy-6’’, 6’’-dimethylpyrano [2’’, 3’’: 3’, 4’]stilbene along with 5 known stilbenes were isolated. The 7 stilbenes exhibited potent antioxidative and antiglycative activities and varied with structure-activity nature. Based on the resultant survival curves and average lifespans of both mouse models, basal diets supplemented with BPSP are effective to extend mouse longevity by a dose dependent manner. It is of merit to demonstrate that peanut kernels as a potent producer could be bio-elicited to biosynthesize a broad spectrum of bioactive stilbenes to prepare BPSP which is effective to extend mouse longevity as science-evidenced by the two long-term animal experiments.

Zhejiang Manufacturing Industry＇s Growing Up and Elicitation

Zhejiang Province firmly seized the opportunity of worldwide economy integration and manufacture industry strategically transfer to renew the ideas, execute the measures and forge ahead innovation so that it practically .developed the superiority of private enterprise system, government service, industry zone, enterprise competition, commodity brand, and culture and ideas and soon formed six manufacture development modes： grass root economy mode, block economy mode, demi-wolf economy mode, scaled economy mode, marketing economy mode and extroverted economy mode, which help Zhejiang province be a big manufacture province and economic prosperous province in China.

Creating Space for Children’s Voices: Utility of the Collage Life Story Elicitation Technique

A challenge for many School-Based Family Counseling(SBFC)practitioners,child psychotherapists and researchers are finding ways to give voice to children and eliciting trustworthy and detailed narratives that could serve as resource for understanding the needs of young clients in the context of all their interpersonal networks.Children are often reticent when asked to self-disclose and tell their stories during consultation.The purpose of the present study was to examine the utility of five sequential steps constituting the Collage Life Story Elicitation Technique(CLET)for scaffolding storytelling among children in middle childhood(aged 9-12 years).Using the CLET for data collection and conducting an interpretive analysis,the researchers explored the performance of 38 middle-childhood children living in three different settings.Findings suggest that the five sequential steps of the CLET adequately and satisfactorily combine to stimulate and elicit rich data and help children to construct their narratives and represent the challenges they face in everyday living.We discuss the application of CLET in SBFC practice as tool when screening and intervention planning for children’s perspectives pertaining to a range of topics regarding each of the four quadrants as proposed in the SBFC metamodel.

The Use of an Elicited Imitation Test to Measure Global Oral Proficiency of L2 Chinese at the Postsecondary Classroom Level

With a growing number of foreign language studies on proficiency outcomes,it is imperative to address the challenge of measuring students’proficiency development in a language program where standardized proficiency testing is not readily available.This article reports administering a Chinese elicited imitation test(EIT)by an instructor to track students’global oral proficiency development in a small language program in a mid-size U.S.public university.The test results from the EIT of second language(L2)Chinese suggest that this tool can provide the instructor with valuable insights into students’oral proficiency.This study also discusses the potential practical value of using this EIT in a language program with limited resources for standardized proficiency assessment.The hope is that this study will encourage language educators who are not already doing so to start using empirical evidence from a valid and reliable proficiency measurement tool to reflect on,improve,and guide their instructional practices.

Lanthanum elicitation on hypocrellin A production in mycelium cultures of Shiraia bambusicola is mediated by ROS generation

Rare earth elements(REEs)have been widely applied as efficient elicitors to improve production of biologically important secondary metabolites in plants.However,their effects on microbial secondary metabolites were seldom reported.In this study,lanthanum(LaCl3)was used as an elicitor in mycelium cultures of Shiraia bambusicola to stimulate the production of hypocrellin A(HA),a promising photosensitizer for anticancer photodynamic therapy.La^3+at 1.0 g/L not only increases HA content by 2.50-fold in mycelia,but stimulates HA exudation to the medium with the highest production(225.05 mg/L)on day 6.Further analysis shows that La^3+can induce the intracellular ROS(reactive oxygen species)accumulation with 2.33-fold higher contents of H2O2 in mycelium.HA production induced by La^3+treatment can be suppressed by ROS scavengers.Further analysis reveals that the elicitation of La^3+on HA accumulation is mediated by ROS generation to enhance expression of HA biosynthetical genes.The expression of ATP-binding cassette transporter gene(SbABC)for HA exudation is also regulated partially via ROS generated by NADPH oxidase.These results indicate that the enhanced HA production by La^3+might be mediated by ROS signaling pathway.Our results provide a basis for understanding REE elicitation on fungal metabolites and a practical biotechnological process for enhanced production of HA.

The RIP3-RIP1-NF-κB signaling axis is dispensable for necroptotic cells to elicit cross-priming of CD8^(+) T cells

Apoptosis and necroptosis are two types of programmed cell death with distinct morphological features.Necroptosis has been assumed to be more inflammatory than apoptosis,but a recent paper1 concluded that necroptotic cells cannot elicit cross-priming of CD8^(+)T cells and that NF-κB activation in necroptotic cells is required for maximal CD8^(+)T-cell cross-priming.

ON DIFFERENT TYPES OF OUTPUT AND THE ELICITATION OF OPTIMAL OUTPUT

By differentiating four different types of output, the paperargues that their roles are also different. And learners of differ-ent language proficiency levels should be guided to produce dif-ferent types of output. Thus the paper introduces a new concept,namely "optimal output". Blindly making the learner produceoutput which is far beyond his current English level will only doharm to his interlanguage development, making it fossilize at anearly stage.

闽南方言外来词视角下泉州华侨文化游客感知元素研究

在实施《“十四五”旅游业发展规划》和建设“海丝文化”的背景下,海外华侨与祖国往来日益频繁和密切,华侨寻根旅游已成为国内旅游业尤其是侨乡文化旅游关注的热点。文章基于旅游凝视理论,将“感知元素”引入华侨文化研究领域,选取著名侨乡泉州的华侨文化为感知对象,以闽南旅游归侨为受试样本,通过深度访谈和文本分析,运用ZMET隐喻抽取技术和词频分析法提取14个起始构念、14个连接构念和11个终结构念,识别其共识逻辑链,构建华侨游客对华侨文化感知元素的共识地图,并据此探究闽南华侨文化元素宣传和宜游化的新举措。

ChatModeler:基于大语言模型的人机协作迭代式需求获取和建模方法

需求获取和建模是需求工程中的关键步骤,影响后续系统设计与实现.传统的需求获取和建模方法通常由需求提供者、需求分析师等多类干系人共同协作、反复迭代完成,需要耗费大量的人力.如何减轻需求提供者与需求分析师的负担、提高获取和建模的效率有着重要意义.现有工作中有的使用知识库来提供更多知识,以辅助获取或者建模,有的利用自然语言处理等技术对获取或者建模过程进行自动化,但是它们并没有减轻需求提供者的负担.利用大语言模型(large language models,LLMs)的生成能力,提供了一种人机协作的迭代式需求获取和建模框架ChatModeler.具体来说,根据真实世界中需求团队的分工及协作关系,将部分需求提供者、需求分析师等角色的工作由大语言模型承担,而需求提供者只需要进行确认.为大语言模型扮演的各种角色进行了提示词设计,该提示词会随需求的元模型而变化.ChatModeler在7个需求案例上与3种需求模型的自动建模方法进行了14组对比实验,证明了ChatModeler在降低需求提供者的负担和生成高质量需求模型2个方面上的优越性.

基于“4Y到位”的照片启发访谈随访对卒中后认知障碍病人照顾者决策疲劳的影响

目的:探讨基于“4Y到位”的照片启发式访谈随访对卒中后认知障碍病人照顾者决策疲劳的影响。方法:2022年1月—11月选择桂林市社区居住的67名卒中后认知障碍病人照顾者为研究对象,随机分为对照组34人和干预组33人,对照组实施常规的微信随访,干预组基于“4Y到位”照片启发技术的随访,即计划到位(yes plan)、责任到位(yes duty)、检查到位(yes check)、激励到位(yes drive),干预3个月后,以决策疲劳量表评价干预效果。结果:干预3个月后,两组照顾者决策疲劳得分均低于干预前(P<0.05),且干预组照顾者决策疲劳得分[(17.79±1.95)分]低于对照组[(20.65±2.77)分],差异有统计学意义(P<0.001)。结论:基于“4Y到位”的照片启发访谈随访能够有效降低卒中后认知障碍病人照顾者的决策疲劳。